Objective: To observe the clinical effects of pediatric tuina plus Chinese medicine for exogenous fever in children. Methods: A total of 150 children withexogenous fever were randomly divided based on the random digital table into a control group (75 cases) and a treatment group (75 cases). The control group was treated with oral Xiao'er Chaigui Tuire Keli (<1 year old, 0.5 bag/time; 1-3 years old, 1 bag/time; 4-6 years old, 1.5 bags/time), 4 times/day. The treatment group was treated with pediatric tuina plus the intervention of the control group. The amount and usage of Chinese medicine were the same as those of the control group; tuina was conducted 1 time/day. The clinical effects and adverse reactions were observed after 3 d of treatment in both groups. The recurrence was observed within 7 d after the end of treatment. Results: The total effective rate was 92.0％ in the treatment group and 81.3％ in the control group. The difference between the two groups was statistically significant (P<0.05). There were no obvious adverse reactions in the two groups after treatment. The recurrence rate was 1.5％ in the treatment group and 13.1％ in the control group. The difference in the recurrence rate between the two groups was statistically significant (P<0.05). Conclusion: Pediatric tuina plus Chinese medicine is effective in treating children with exogenous fever.

OBJECTIVETo Study the effect of anti-aging and its mechanism of total saponins of Wu-He Dipsacus asper on skin of mice-aging model.

METHODSForty-eight mice were randomly divided into blank control group, model group, low-Dipsacus group, medium-Dipsacus group, high-Dipsacus group and positive control group( n = 8) . The mouse model of skin aging was established by nape subcutaneous injection of 5% D-galactose (0.025 mL/(g · d)), the mouse of low-Dipsacus group, medium-Dipsacus group, high-Dipsacus group were administered with total saponins of Wu-He Dipsacus asper (50 ml/(kg · d), 100 mL/(kg · d), 200 mL/(kg · d)), the mice of the positive control group were administered with vitamin E(50 mg/(kg · d)) for 42 d. The content of hydroxyproline (HYP) and lipofuscin (LF) were measured in skin of each group mice, the activity of catalase (CAT) glutathione peroxidase ( GSH-Px) superoxide dismutase (SOD) and the content of malondi- aldehyde (MDA) were determined in serum and skin of each group mice.

RESULTSCompared with blank control group, the content of HYP decreased significantly and the content of LF increased significantly in skin, the activities of CAT, GSH-Px and SOD decreased significantly and the content of MDA increased significantly in serum and skin of model group; Compared with model group, the content of HYP increased significantly and the content of LF decreased significantly in skin, the activities of CAT, GSH-Px and SOD enhanced significantly and the con- tent of MDA decreased significantly in serum and skin of low-Dipsacus group, medium-Dipsacus group, high-Dipsacus group and positive control group; Compared with low-Dipsacus group, the content of HYP increased significantly and the content of LF decreased significantly in skin, the activities of CAT, GSH-Px and SOD enhanced significantly and the content of MDA decreased significantly in serum and skin of high-Dipsacus group and positive control group; The activity of SOD in serum and skin had a significant positive correlation with the content of HYP, and a significant negative correlation with LF in skin.

CONCLUSIONTotal saponins of Wu-He Dipsacus asper have obvious effect of anti-agng on skin of mouse-aging model , its mechanism is closely related to oxidative damage.

Animals ; Dipsacaceae ; chemistry ; Disease Models, Animal ; Mice ; Oxidative Stress ; Saponins ; pharmacology ; Skin Aging ; drug effects

China ; Curriculum ; Education, Dental ; Oral Medicine ; education

Adult ; Esophagus ; surgery ; Female ; Foreign Bodies ; surgery ; Humans ; Male ; Middle Aged ; Neck ; surgery

The ability to induce hypersensity on leaves of tomato and the stability of double-plasmid of an harpin-producing, nitrogen-fixing engineered strain E4 were tested. Hypelsensitivity-inducing experiment indicated that the time and density of hypersensitivity-induction of E4 was similar to those of DH5, the positive control of pCPP430. Although E4 took the same time to induce hypersensitivity as 308R, another positive control of pCPP430, it induced weaker hypersen- sitivity on tobacco leaves. On tomato leaves, there was no difference in time and density of hypersensitivity between E4 and 308R (pCPP430). Results revealed that the two plasmids, pCPP430 and pMC73A, were unstable in host bacteria, with the losing rate of 100% at the 48th generation. The emergence probability of bacteria with either pCPP430 or pMC73A was almost the same.

Dermatitis, Occupational ; etiology ; Humans ; Trichloroethylene

Objective To develop a new type of electrochemical immunosensor for the detection of ochratoxin A (OTA ) . Methods Double layers of self‐assembly immunosensor for the detection of OTA were constructed based on the composite single‐walled carbon nanotubes(SWNTs)/chitosan(CS) membrane immobilized on glassy carbon electrode(GC) .Scanning electron mi‐croscopy(SEM) ,square wave voltammetry and cyclic voltammetry were used to analyze the characterization of the sensor ,then its specificity for detection was studied .Results SWNTs/CS composit membrane could increase the sensitivity of OTA detection sig‐nificantly ,and effectively distinguish the different types of mycotoxins .Conclusion The electrochemical immunosensor developed in the study is easy to operate and could detect OTA rapidly with good specificity and low detection limit .

Objective To analyze whether there are any gender differences in clinical features of acute type 1 diabetes mellitus.Methods Clinical data of 193 patients with acute type 1 diabetes mellitus were collected in our hospital from 2002 to 2007,and subjected to statistical analysis by SPSS.Results The ratio of male to female in all the subjects was 1.6:1.There was no significant gender difference in such clinical aspects as age,fasting blood glucose level,HbAlc concentration,positive percentage of serum autoantibodies against pancreatic islet or morbidity of diabetic neuropathy.In contrast to the males,the onset age was significantly younger,the diabetic duration longer,fasting serum C peptide level lower and the incidence of ketotic acidosis higher in female patients.The patients with onset age less than 14 years (puberty) did not show any significant gender differences in the above-mentioned clinical aspects.Among those with onset age more than 14 years,the females had significantly longer duration and lower fasting serum C peptide level than males.The male patients with onset age more than 14 years had significantly higher fasting serum C peptide level than those less than 14,whereas it was the same for total positive percentages of pancreatic islet autoantibodies and morbidity of diabetic neuropathy in females.Conclusion Gender differences may exist in some clinical features of type 1 diabetic patients with acute onset after puberty, which may partially result from the effects of sex hormones.

AIM: To build an allergic rhinitis model to evaluate the effect of budesonide on the eosinophils and histamine in nasal mucosa in allergic rhinitis guinea pig.METHODS: 33 guinea pigs were randomly divided into three groups: natural controlled group,allergic rhinitis(AR) group and budesonide treatment group.Allergic rhinitis model in guinea pigs were built by using toluene-2,4-disocyanate(TDI) nasal immunization and challenge.The indexes of clinical symptoms,pathomorphological diagnosis and content of histamine in mucosa were used to evaluate the potency of budesonide when used to treat allergic rhinitis.RESULTS: The expression of eosinophils and the content of histamine in nasal mucosa in AR group both were significantly higher than that of in natural controlled group(respectively,P(0.05)).CONCLUSION: Budesonide could effectively reduce the expression of eosinophils and content of histamine in nasal mucosa,but it is invalid in releasing the symptoms of AR in guinea pig.The reason of this phenomenon may be concerned with the method of model building.

Objective: To distinguish malignant from osteoporotic acute vertebral collapses at the single location. Methods Fifteen osteoporotic and sixteen malignant vertebral collapses were studied in thirty - one patients with T1 WI, gadolinium enhanced T1 WI and T2 WI MRI. All the patients had the follow - up periods of 3 - 6 months or pathologic results from biopsy. Results Seven findings were suggestive of osteoporotic: 1. The compression of vertebral body was severe. 2. The lesion extended to the whole vertebral body was uncommon. 3. The location of the lesion was closed to the end plate of the vertebral body and the anterior and /or posterior of the vertebra was straight or concave. The posterior bone fragment can be seen. 4. Pedicles were normal. 5. No epidural soft tissue mass. 6. The vertebral vein was normal. 7. The bandilike or flakelike enhancement under the end plate. Another seven findings were suggestive of malignancy: 1. The compression of vertebral body was mild. 2. The lesion extended to the whole vertebral body was common. 3. The anterior and /or posterior of the vertebra cortex was convex. 4. Pedicles were involoved. 5:Epidural soft tissue mass was usually seen. 6. The vertebral vein was involved and disappeared. 7. The lesion was usually nodulus enhancement. Conclusion To distinguish malignant from osteoporotic acute vertebral collapses,the MRI findings of the morphology and the shape of the lesion postcontrast are useful in the differentiation of solitary acute vertebral collapses.