1.Effect of insulin injection and protamine biosynthetic human insulin injection on basal insulin level in pregnant women with diabetes mellitus
Xuejiao YING ; Rui LIU ; Qian ZHAO
Chinese Journal of Biochemical Pharmaceutics 2016;36(12):97-98,102
Objective To explore the effect of insulin injection and protamine biosynthetic human insulin injection on basal insulin level in pregnant women with diabetes mellitus.Methods Retrospective analysis of 89 cases of pregnant women with diabetes mellitus from January 2013 to May 2016 in department of obstetrics and gynecology,tianjin red bridge hospital,the patients were divided into group A (n=38 cases) and group B (n=51 cases),the group A treatment with insulin injection,the group B treatment with protamine biosynthetic human insulin injection,compare the two groups of patients before and after treatment of three meals a day rate of blood glucose compliance, treatment compliance and satisfaction.Results Before treatment, there was no significant difference in the blood glucose compliance rate between the two groups before and after treatment;After treatment,the blood glucose compliance rate of two groups was significantly higher than before treatment ( P<0.05 ) , there was no significant difference in the compliance rate of fasting blood glucose between two groups,the compliance rate of blood glucose before dinner in group A was 81.58%,significantly higher than that in group B 60.78%(P<0.05).Conclusion Both insulin injection and protamine biosynthetic human insulin injection can maintain the basic insulin levels of pregnant women with diabetes mellitus,insulin injection can better control the blood glucose levels before dinner,with higher compliance and satisfaction.The compliance rate and satisfaction rate of pregnant women in group A were 97.37% and 97.37%,which were significantly higher than those in group B 82.35% and 80.39%(P<0.05).
2.Expression of tight junction protein occludin in the brain microvascular endothelial cells of rats with acute necrotizing pancreatitis
Ying LIU ; Jun LIU ; Wei QIAN ; Xiaohua HOU
Chinese Journal of Pancreatology 2008;8(5):326-328
Objective To explore the changes of expression of tight junction protein occludin in the brain microvascular endothelial cells of rats with acute necrotizing pancreatitis (ANP), and to study the relationship between the occludin expression and degree of pancreatic pathologic changes. Methods 80 rats were randomly divided into sham-operation (SO) group and ANP 3 h, 6 h, 12 h, and 24 h groups. Retrograde pancreatic duct injection of 5% choleate sodium in rats was used to establish ANP models. Pancreatic pathologic changes was evaluated. Immunohistochemistry, Western blot and RT-PCR were used to determine the expressions of the tight junction protein occludin in the brain microvascular endothelial cells. Results Occludin protein was linearly expressed along brain vascular vessels. The expression of occludin protein at 3h was 0. 35±0.09 in brain tissues from rats with ANP and 0. 49±0.08 in SO group, the expression of occludin mRNA were decreased from 1.50±0.30 to 1.01±0.18 (P < 0.05), the lowest expression rate occurred at 6 h, and the values in the two groups were 0. 26±0.07 and 0. 93±0. 19, respectively, the expressions of occludin protein and mRNA of ANP group at 12 h, 24 h were notably increased when compared with those at 6 h (P < 0. 05), but they were still lower than those in SO group (P < 0.05). The occludin protein and mRNA expressions were negatively related with the degree of pancreatic pathologic changes (Pearson correlation coefficient: -0.48 and -0.536, P < 0.05). Conclusions The expression of tight junction protein occludin decreased at both protein and mRNA level in brain microvascular endothelial cells of ANP rats. Furthermore, the expression of occludin was negatively correlated with pathological change of pancreas.
3.Subacute stent thrombosis after drug-eluting stent implantation for treatment of bare metal stent associated very late stent thrombosis.
Ming LIU ; Xue-bo LIU ; Ju-ying QIAN
Chinese Journal of Cardiology 2008;36(2):175-176
Coronary Restenosis
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etiology
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Humans
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Male
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Middle Aged
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Myocardial Infarction
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therapy
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Stents
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Thrombosis
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etiology
4.Effects of Zuogui pill, Yougui pill and relative compositions on differentiation towards germ cells of mouse embryonic stem cell 1B10.
Zou-ying YAO ; Qian WAN ; Hua LU ; Xia LIU
China Journal of Chinese Materia Medica 2015;40(3):495-500
OBJECTIVETo investigate the effects of Zuogui pill, Yougui pill and the relative compositions on the differentiation towards germ cells of stem cells.
METHODThe rat drug sera for Zuogui pill, Yougui pill and the common composition of Zuogui pill and Yougui pill were prepared respectively as the experimental drugs; the mouse embryonic stem cell 1B10 (MESC-1B10) was used as the representative of stem cells; the above rat drug sera were used to intervene MESC-1B10 and the process was traced by microscopy imaging; after 72 h of the intervention, the RNAs were extracted from the different intervened MESC-1B10, cDNAs were synthesized immediately and finally the Real-time quantitative PCR (qPCR) was used to measure the expression patterns of the 10 reproductive-differentiation-related genes for each intervention.
RESULTThe rat drug serum of Zuogui pill (ZGW-RS) significantly up-regulated Oct-4 and SCP3 and significantly down-regulated GDF-9 and Stra8; the rat drug serum of Yougui pill (ZGW-RS) significantly up-regulated Oct-4, GDF-9, Mvh and SCP3 and significantly down-regulated Stra8, Itga6 and Itgb1; the rat drug sera for the common composition of Zuogui pill and Yougui pill (ZGWYGW-RS) significantly up-regulated Oct-4, SCP3 and ZP3 and significantly down-regulated GDF-9, Stra8, Itga6 and Itgb1.
CONCLUSIONZGW-RS can initiate the change towards meiosis, but can not start the reproductive differentiation of MESC-1B10; YGW-RS can initiate the change towards meiosis, and can also start the reproductive differentiation of MESC-1B10 towards female germ cells; ZGWYGW-RS can initiate the change towards meiosis, and can lightly start the reproductive differentiation of MESC-1B10 towards female germ cells but the inductive effect is smaller than YGW-RS. The experimental results, on one hand, strengthen the knowledge about the influence of the relative compositions of Zuogui pill and Yougui pill on the reproductive differentiation of stem cells, on the other hand, help to explain the mechanism of the treatment of the infertility by Zuogui pill and Yougui pill.
Animals ; Cell Differentiation ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Embryonic Stem Cells ; cytology ; drug effects ; Female ; Germ Cells ; cytology ; drug effects ; Infertility ; drug therapy ; Male ; Mice ; NIH 3T3 Cells ; Rats ; Rats, Sprague-Dawley
5.Improving teaching quality of oral and maxillofacial surgery with diversified discussion teaching
Zhiguo LIU ; Qian DONG ; Ying CHEN ; Haikuo TANG
Chinese Journal of Medical Education Research 2016;15(11):1154-1157
To improve the students'! comprehensive quality and cultivate their innovative medical talents, we applied different forms of discussion teaching in different stages of the students. In the course of theory, the form of short discussion was adopted to improve the students'! interest and concentration. In preclinical practice stage, the method of problem centered discussion was used to consolidate the profes-sional knowledge of the students. Discussion in the clinical practice was based on the actual problem of diagnosis and treatment, in order to cultivate the students'!clinical thinking. Through the diversified practice of discussion teaching with positive teaching evaluation, students'!interest in oral and maxillofacial surgery and the quality of teaching were improved.
6.Clinical Observation of S-1 Capsules Combined with Recombinant Human Endostatin in the Treatment of Middle and Advanced Primary Liver Carcinoma
Jin SU ; Kezhi SHI ; Yang LIU ; Ying QIAN ; Xinhua XU
China Pharmacy 2017;28(11):1496-1499
OBJECTIVE:To observe therapeutic efficacy and safety of S-1 capsules combined with recombinant human end-ostatin in the treatment of middle and advanced primary liver carcinoma. METHODS:Totally 94 patients with middle and advanced primary liver carcinoma in the First College of Clinical Medical Science of China Three Gorges university during Feb. 2012-Dec. 2014 were divided into combination group(48 cases)and control group(46 cases)according to random number table. Both groups were given S-1 capsules 40-60 mg orally within 30 min after breakfast and supper. Combination group additionally received Recom-binant human endostatin injection 150 mg added into 0.9%Sodium chloride injection 210 mL with portable micro pump for continu-ous pump of 120 h. A course involved 14 d treatment and 7 d interval. Short-term objective therapeutic efficacy,clinical benefit re-sponse (CBR) and ADR were evaluated after 2 courses. Disease progression time and average survival period were compared be-tween 2 groups. RESULTS:Objective response rate,disease control rate,disease progression time and average survival period of combination group were 14.6%,66.7%,(5.5 ± 1.3) months,(10.7 ± 3.8) months;those of control group were 8.7%,45.6%, (4.8±1.2)months,(8.9±3.3)months,with statistical significance between 2 groups(P<0.05). CBR rate of combination group (79.2%)was significantly higher than control group(52.2%),with statistical significance(P<0.05). There was no statistical sig-nificance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:S-1 combined with recombinant human end-ostatin show good therapeutic efficacy and tolerance for patients with middle and advanced primary liver carcinoma,and do not in-crease the incidence of ADR.
7.Detection and Handling of Carry-over of Automatic Biochemistry Analyzer
Ling QIU ; Xinqi CHENG ; Qian LIU ; Ying SHEN
Journal of Medical Research 2006;0(06):-
Objective To investigate the detection and treatment of carry-over of automatic biochemistry analyzer. Methods We detected the sample carry-over by determining one high level quality control serum and three zero levels serum continuously in five days. We used mixed patients serum to detect the carry-over come from reagent probe, muddler and cuvette by arranging test sequence of different item, combination and the times of rerun. We also used different methods such as immersion, cleaning probe, extended priming to handle the reagent carry-over.Results The sample carry-over was -0.37%~0.48% on new analyzer and 0.51~1.17% on old analyzer, respectively. The carry-over of CHO, TG, HDL on reagent probe and muddler of TBA was 86.9%~208.7% and 86.0%~256.5% on old analyzer, respectively. There was no reagent carry-over among other items on old analyzer. There was no carry-over on reagent probe on new analyzer, the carry-over of different items on cuvette of TBA was -0.9%~1.7%. After immersion, cleaning and extended priming, the carry-over of CHD, TG, HDL on TBA reagent probe fell to -1.1%~2.7% and -0.07%~0.09%, respectively.Conclusions The methods which have been mentioned above can detect the sample and reagent carry-over. The carry-over can be reduced by immersion, cleaning, extended priming and testing in different unit.
8.Advances in Application of Blue Laser Imaging in Diagnosis of Early Upper Gastrointestinal Cancer
Ying ZHUO ; Haifeng KANG ; Junbo QIAN ; Jianwei QIU ; Hongbin LIU
Chinese Journal of Gastroenterology 2017;22(6):377-380
Blue laser imaging (BLI) is a new endoscopic system equipped with the laser beam emitting two different wavelengths.It produces bright and high resolution images for observation of microvascular and microsurface patterns of esophageal and gastric mucosa, helping the diagnosis of early upper gastrointestinal cancer.Compared with the existed endoscopic techniques, BLI shows its unique advantages.In this article, advances in application of BLI in diagnosis of early upper gastrointestinal cancer were reviewed.
9.Clinical and Pathological Analysis of 401 Cases by Mucosa Biopsy
ying-zi, TANG ; hai-yan, WU ; xiang-qian, LIU
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To explore the clinical and pathological feature of pediatric chronic gastritis and whether gastritics in children related to hericobacter pylori(Hp).Methods Retrospective analysis the result of 401 children with gastroendosopy and 100 species with Hp detected were performed.Results Superficial gastritis form mild to moderate was the mainly pathologic change in pediatric chronic gastritis,chronic inflammation was the mainly change in pediatric Hp infection,and the detected rate of chronic superficial gastritis from mo-(derate) to heavy was significantly higher than mild(?~2=7.61 P
10.The effects of endotoxin on the Th1/Th2 cells and immune modulation of Astragalus membranaceus.
Jian-hua LIU ; Ying-qian ZHANG ; Bo HUANG ; Qian FANG ; Ximin HUO ; Haofu HU
Chinese Journal of Pediatrics 2003;41(8):613-614
Animals
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Astragalus membranaceus
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Endotoxemia
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drug therapy
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metabolism
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Interferon-gamma
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analysis
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Interleukin-4
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analysis
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Male
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Mice
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Phytotherapy
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Plant Extracts
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therapeutic use
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Th1 Cells
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drug effects
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metabolism
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Th2 Cells
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drug effects
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metabolism