Suture was a traditional method for tissue repair. However, sutures used in the closure of surgical wounds can induce inflammation and lead to scarring that appears as crosshatch marks. Threads used for sutures may cause immune response and surgical infection. In this review, we introduce a novel sutureless technique for wound repair, called photochemical tissue bonding (PTB). Absorption of visible light by a photosensitizing dye initiates photochemical reactions leading to covalent protein-protein crosslinks that bridge the wound surfaces, thus producing an immediate seal between the wound surfaces. This technique has been applied in various tissues including skin, cornea, nerve, blood vessels, et al. PTB reduces inflammation resulting in less scarring in skin. PTB dramatically improves the incidences of vascular anastomotic leakage and stricture. In addition, PTB enhances the recovery of nerve function after nerve repair.

Adenocarcinoma ; metabolism ; pathology ; Aged ; Carcinoid Tumor ; metabolism ; pathology ; Chromogranin A ; metabolism ; Humans ; Immunohistochemistry ; Male ; Neoplasms, Multiple Primary ; metabolism ; pathology ; Phosphopyruvate Hydratase ; metabolism ; Rectal Neoplasms ; metabolism ; pathology ; S100 Proteins ; metabolism

Objective To determine the quality of life of patients treated with one of three different types of surgery for breast cancer.Methods This was a cross-sectional study using a questionnaire survey completed by Chinese patients without active disease after at least 2 years of follow-up after breast cancer surgery.Results This study totally included 139 breast cancer patients:44 ( 31.6%) had undergone modified radical mastectomy with reconstruction, 41(29.5%)had a quadrantectomy with axillary lymph node dissection, and 54(38.9%)had a modified radical mastectomy without reconstruction.The EORTC QLQ-C30 and EORTC QLQ-BR23 question-naires were used;their reliability was>0.82.Global health status(94.30 ±12.04, P=0.028)and role functio-ning(85.16 ±17.23, P=0.138)were highest in the quadrantectomy group.Pain score was highest in the modi-fied radical mastectomy with reconstruction group(26.13 ±30.15, P =0.042).The breast symptom score (22.56 ±22.30, P=0.009)and body image perception(85.56 ±19.72, P=0.025)were highest in the conser-vative treatment group.The overall health of patients given modified radical mastectomy without reconstruction was lower(72.61 ±20.89, P=0.014) in women older than 50 years compared with younger women.Conclu-sions The quadrantectomy with axillary lymph node dissection procedure had better acceptance, but the overall health status did not differ between groups.Overall health status is lower in women older than 50 years receiving a modified radical mastectomy without reconstruction.

Objective The aim of this study was to investigate the correlation of serum copper levels and mental health in psychiatric shift nurses.Methods The participants were divided into day shift nurses(control group,n=60) and night shift nurses(n=60).The concentrations of trace mineral,serum biochemical levels,inflammatory and oxidative markers in blood samples of 120 shift nurses were measured.The depressive mood was measured with second edition of Chinese version of the Beck Depression Inventory,(BDI-Ⅱ).Results The night shift nurses showed moderate-to-severe depression levels,which were significantly higher than those of the day shift nurses,23.3％ (14/60) and 11.7％ (7/60) vs.6.7％ (4/60)and 1.7％ (1/60),x2=6.536,4.82,P ＜ 0.05.Night-shift nurses also had higher levels of serum copper,hs-CRP,IL-6,T-SOD and Cu-Zn SOD than the control group,(19.57±4.14) μmol/L vs.(18.03±3.20) μmol/L,1.32(0.78-1.89) rng/L vs.0.89(0.45-1.44) mg/L,68.54 (51.43-96.78) ng/L vs.56.39 (35.56-78.71) ng/L,(66.78±14.72) kU/L vs.(47.32±17.84) kU/L,(54.22±14.91) kU/L vs.(48.17±17.23) kU/L,t=-3.133～-2.057,P ＜0.01 or 0.05.BDI-Ⅱ scores correlated positively with serum copper,hs-CRP,IL-6,T-SOD and Cu-Zn SOD,r=0.248-0.457,all P ＜ 0.01.In night shift group,multivariate regression analysis showed that serum copper,hs-CRP,IL-6 and T-SOD (β=0.097-5.340,P＜ 0.01 or 0.05) were independently related factors influencing BDI-Ⅱ scores (R2 =0.609,P =0.000).Conclusions Serum copper may serve as an evaluation index for depressive mood in psychiatric shift nurses,and serum copper concentrations in night shift nurses should be monitored regularly.Clinical Trial Registry Chinese Clinical Trial Registry,ChiCTR-OOC-14005540.

OBJECTIVE:To explore the relevant factors that led to the adverse drug reaction(ADR)of netilmicin.METHODS:ADR cases resulted from netilmicin reported in12kinds of Chinese periodicals from1998to2003were analyzed statistically.RESULTS:The ADR of netilmicin involved multiple organs and systems,the clinical manifestation were compli-cated and diversified,which manifested mainly on the renal toxicity,toxicity of nervous system and allergic reaction.CON-CLUSION:Clinician and pharmacists should be alert to the ADR of netilmicin and keep using drugs rationally.

A myxobacterial strain, So ce cpu-1, was isolated from a soil sample near the Huanghai beach. So ce cpu-1 had broad antimicrobial activity, active component has maxima] absorption at 210 nm. The effects of different culture conditions on the yield of the secondary metabolites were investigated. The results showed that, when cultivating the strain in the M, medium (containing 10% w/v D312 neutral absorber resin ), the air up to 70% of the whole flask volume, adding 5?L the secondary metabolites as the revulsant, at 30℃, 200r/min, for 6 days, the yield of the secondary metabolites achieved the maximum.

Objective To investigate the expression of the ER,CyclinD1 in breast tissue of pubertal mammary hypertrophy and explore the possible etiology of pubertal mammary hypertrophy.Methods The expression of ER,CyclinD1 was detected by Western-blot in 15 cases of pubertal mammary hypertrophy,15 normal breast tissue and 10 cases of micromastia.Results There was significant difference of the expression of ER,CyclinD1 within breast tissue between pubertal mammary hypertrophy and micromastia(P＜0.01).There was significant difference of the expression of ER,CyclinD1 within breast tissue in pubertal mammary hypertrophy and in normal breast tissue(P＜0.01).There was no difference between the expression of ER,CyclinD1 within breast tissue in micromastia and in normal breast tissue(P＞0.05).Conclusions The expression of ER,CyclinD1 in pubertal mammary hypertrophy are significantly higher than in micromastia and normal breast tissue.The pubertal mammary hypertrophy may be related to the expression status of ER,CyclinD1 within breast tissue.

Objective To evaluate gene associated with retinoid-interferon-induced mortality-19 (GRIM-19) expression levels and the correlation with its target gene product signal transducers and activator of transcription 3 (STAT3)in human breast cancer tissues and normal gland tissues,and to analyze their roles in the tumorigenesis of breast cancer.Methods The expression of GRIM-19 and STAT3 protein and mRNA in 40 cases of breast cancer tissues and 40 cases of normal gland tissues was detected by immunohistochemistry and Western blot.The correlation of the expression of GRIM-19 and STAT3 to various clinicopathologic characteristics of breast cancer were analyzed statistically.The mRNA expression and gene mutation of GRIM-19 in breast cancer cell line MCF-7 and 25 specimens of breast cancer and normal gland tissue were detected by reverse transcription-polymerase chain reaction(RT-PCR)and sequencing.Results The protein and mRNA expression of GRIM-19 was obviously lower in breast cancer than in normal gland tissues (P ＜ 0.05) while the protein and mRNA expression of STAT3 was obviously higher in breast cancer than in normal gland tissues(P ＜0.05).The expression of GRIM-19 and STAT3 was negatively correlated with each other(x2 =8.25,P ＜0.01).Breast cancer samples exhibited low level of GRIM-19 and moderate to high level of STAT3 expression.In contrast,the normal gland tissue was characterized by high level of GRIP-19 and low level of STAT3 expression.The protein expression of GRIM-19 was correlated with the histological grading and clinical stage of breast cancer(P ＜ 0.05).STAT3 was not correlated with clinicopathologic characteristics of breast cancer (P ＞ 0.05).No mutation of GRIM-19 gene was detected in breast cancer tissues,normal gland tissues or MCF-7 breastcancer cells.Conclusions The low expression of GRIM-19 and the high expression of STAT3 co-exist in breast cancer.Downregulation of GRIM-19 was closely correlated with increased histological grade,clinical stage and STAT3 in breast cancer.The potential role of GRIM-19 in breast cancer development may be through these correlations.

Objective To evaluate the changes of ventricular synchrony in patients with acute myocardial infarction(AMI)before and after percutaneous coronary intervention(PCI)by speckle tracking imaging(STI).Methods Thirty patients with AMI were examined by traditional echocardiography and STI before and 5 d,1 month after PCI.Myocardial imaging of left ventricular apical long views and parasternal left ventricular short axis views were recorded and stored.The time to peak longitudinal systolic and diastolic strain rate(TS-SRL,TE-SRL)and to peak radial systolic and diastolic strain rate(TS-SRR,TESRR)were measured respectively.The differences of the maximum and minimum systolic and early diastolic peak time(TSL-diff,TER-diff)and the standard deviation(TSL-SD,TER-SD)in 18 longitudinal segments were calculated.And the differences and standard deviation of TS-SRR and TE-SRR(TSR-diff,TSR-SD,TER-diff,TER-SD)were calculated also.These parameters were compared before and after PCI.Results Compared with before and 5 d after PCI,the values of TSL-diff,TSL-SD,TEL-diff and TEL-SD in 1 month after PCI were decreased significantly(P＜0.01).TSR-diff,TSR-SD,TER-diff and TER-SD were decreased also and the differences had statistical significance(P＜0.01).The cardiac synchronized index and LVEF values showed good negative correlation.Conclusions The systolic and diastolic synchrony of left ventricle in AMI patients was abnormal.STI can evaluate left ventricular synchronized function and the changes before and after PCI to assess the effect of PCI.

Aim: To elucidate the structure of an acidic polysaccharide from Cordyceps sinensis and to study its protective effect on renal cell injury by cisplatin. Methods: After Cordyceps sinensis acidic polysaccharide( CAPS) was isolated and purified, the purity and molecule weight were detected by high performance gel permeation chro-matography( HPGPC). Based on the UV spectrum, FT-IR spectrum, monosaccharide composition and GC-MS, the structures were elucidated. Cisplatin-induced injury of Madin-Darby canine kidney( MDCK) cell line was protec-ted by the blood serum with CAPS. Results: The purity of CAPS, with a molecule weight of 2. 7×10~4, was about 99%. It consisted mainly of mannose, glucose and galactose. When the serum with CAPS reached 2.5%, it showed difference compared with the cisplatin group (P <0. 05) on the protective effect of renal cell injury. It showed significant difference( P <0.01) when the serum with CAPS exceeded 5%. Conclusion: CAPS has a pro-tective effect on the renal cell injury in vitro.