The paper introduces using MedlineR to carry out data mining from Medline, for observing the gene relation, and using Pajek to realize the visualization of the results. Practice proofs that MedlineR could help researchers come up with assumptions in the field that they are interested in, and get a visual fingerprint. These results are the bases to go to more complex literature data mining and an important supplementary to experimental methods.

OBJECTIVE:To compare the percutaneous absorbency between imported and domestic5%doxepin cream.METHODS:In a cross design,8health male volunteers were enrolled in the study.Determination was performed by HPLC with chlorimipramine as internal standard.RESULTS:The same dose of domestic and imported doxepin cream was topically applied to the forearm skin of the volunteers for8consecutive days.The blood drug levels were very low in both groups without significant difference.CONCLUSION:The percutaneous absorbency of domestic5%doxepin cream can reach that of imported ones and the domestic cream is safe and effective.

Objective To explore the the cellular damage of hippocampus neuron in a rat model of chronic cerebral hypoperfusion. Methods Rat model of chronic cerebral hypoperfusion was established by permanent bilateral common carotid arteries occlusion (2VO). Eight weeks after the operation,the brains were removed and examined with histological stains, electron microscope, flow cytometer and Western Blotting. Results Compared with the control group,the arrangement of hippocampus neurons in 2VO rats appeared to be more irregular, and the number of the neurons decreased partly ( CA2: ( 34.75 ± 3.40) vs (49.25 ± 9.67 ), P ＜ 0. 05; DG: ( 73.50 ± 9.26)vs ( 90.75 ± 4.35 ), P ＜ 0. 05 ). By electron microscopic study of hippocampus neurons in 2VO rats, the nuclei became smaller and the heterochromatin assembled in the border of the nuclei in some neurons, while cytoplasm swelled,especially in mitochondria and endoplasmic reticulum. The rate of apoptosis of hippocampus neurons in 2VO rats( (9. 117 ±2. 540)% ) ,detected by the flow cytometer,was higher than that of sham group( (4. 750 ±3.481 ) % ) (P ＜ 0. 05 ). The expression of pro-caspase-3 in hippocampus of 2 VO rats was not altered significantly compared with the control group(P ＞ 0. 05 ). Conclusion The cellular damage of hippocampus neuron in 2VO rats was mainly caused by apoptosis.

Objective To investigate the clinical characteristics,pathogenic causes and treatment outcomes of acute kidney injury (AKI) in very old patients in order to improving the diagnosis and treatment.Methods 39 AKI patients aged ≥80 years in the department of geriatric medicine of our hospital were enrolled and followed up for 3 months.Clinical and laboratory data were analyzed and the pathogenic causes as well as the treatment outcomes were summarized.Results Among the 39 very old patients,31(79.5％) were male,8(20.5％) were female,and the mean age was (93.5±4.6) years.33 (84.6％)patients had chronic kidney diseases.The pathogenic causes of AKI were mainly as follows:urological infections (71.8％),blood volume deficiency (48.7％),cardiac dysfunction (23.1％) and hypotension (15.4％).2 to 4 pathogenic causes were co present in 64.1％ patients.Renal function was improved and recovered in 69.2 ％ patients and the death rate was 25.6％ after treatment.The main causes of death were septic shock (30.0％),multiple organ failure (20.0％),massive gastrointestinal hemorrhage (20.0％) et al.Conclusions Most of the very old patients may suffer from AKI on the basis of chronic kidney disease.AKI is often induced by multiple pathogenic causes.The main factors leading to AKI are urological infections,followed by blood volume deficiency and cardiac dysfunction.Most patients with AKI can recover after the precipitating factors are removed and supportive treatments are administered in time.Septic shock is the main cause of death.

Objective To observe the effect of resveratrol on cognitive impairment in rats after chronic cerebral hypoperfusion,and to explore the underlying antioxidant mechanism of resveratrol.Methods The chronic cerebral hypoperfusion model was induced by permanent occlusion of bilateral common carotid arteries (2VO) in rats.Healthy male Wistar rats were randomly divided into 3 groups:sham-operated group,2VO group and 2VO+resveratrol group.Spatial learning and memory were assessed using the Morris water maze at 4 weeks after the occlusion.The levels of 4-Hydroxynonenal (4-HNE) and 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in the cortex and hippocampal CA1 areas were detected using immunohistochemistry staining,for reflecting the lipid peroxidation and oxidative DNA damage.Results The escape latencies from the third day to the fifth day were longer in 2VO group than in sham-operated group[(42.1+5.4)s vs.(25.1±3.3)s,(36.4±4.4)s vs.(12.4±3.3) s,(30.4±4.0)s vs.(8.1±3.4)s,q=10.91、14.54 and 14.07,all P ＜0.01],while the time spent in the object square was shorter in 2VO group than in sham-operated group[(12.9+2.5)s vs.(18.9+2.2)s,q=6.47,P＜0.01].Compared with 2VO group,the escape latencies in 2VO+resveratrol group from the third day to the fifth day were shorter[(29.5+4.0)s,(25.6±4.3)s and (19.8±4.2)s,q=7.71,6.22 and 6.37,all P＜0.01],while the time spent in the object square was longer[(16.5±1.8)s,q=3.83,P＜0.05].Compared with the shamoperated group,the mean integral optical density (IOD) of 4-HNE and 8-OHdG in the cortex and hippocampus CA1 area were increased in 2VO group (265.1 + 9.0 vs.168.2 + 6.0,37.8 + 5.0 vs.24.0+4.0,q=31.89 and 7.48,both P＜0.01).And in the 2VO+resveratrol group,the mean IOD of 4-HNE and 8-OHdG in the cortex and 8-OHdG in hippocampus CA1 area were lower than in 2VO group (195.1±7.0,26.0±4.3,q=23.03 and 6.49,both P＜0.01).Conclusions Resveratrol can improve the cognitive impairment in rats after chronic cerebral hypoperfusion,which may be related to preventing oxidative damage.

ObjectiveTo observe the effects of enriched environment on depressive-like behaviors induced by chronic stress in rats.MethodsAdult rats were randomly divided into stress group and the control group,then after 21 days of stress,rats were raised in an enrich environment (EE) or standard environment (SE)for 28 days.Open field test,forced swimming test and sucrose preference test were used to detect depressive-like behavior in rats.Results ( 1 ) Open field test showed that grid crossing,standing and rearing in stress ( no gap)rats were significantly reduced ( 13.88 ±4.38 vs 45.00 ± 10.19,9.13 ±2.54 vs 16.38 ±4.11 and 4.78 ± 1.39vs 10.51 ± 2.52 ; n =8 ; P＜ 0.01 respectively) ; but grid crossing and standing in stress + EE rats were significantly increased than stress + SE rats (41.61 ± 10.53 vs 26.25 ± 6.18 and 16.79 ± 3.49 vs 11.25 ± 3.12 ; n =8 ; P＜0.01 and P＜0.05 respectively).(2) Sucrose preference tests showed that sucrose consumption and sucrose preference％ in stress ( no gap) rats were significantly decreased than those in the control group ( 5.22 ± 0.94 vs 10.61 ±2.59 and (49.11 ±6.77)％ vs(63.38 ±8.36)％ ; n=8; P＜0.01 respectively).(3) Forced swimming test showed that immobility time was increased in stress ( no gap) rats ( ( 19.5 ± 5.43 ) s vs ( 12.75 ± 3.9 ) s; n =8 ; P ＜ 0.05 ) and was restored by EE compared to SE rats ( ( 10.25 ± 3.57) s vs ( 17.75 ± 5.45 ) s; n =8 ; P ＜ 0.05).ConclusionChronic stress can reduce depressive-like behaviors,and EE can restore depressive-like behaviors induced by chronic stress.

Objective To study on wavelet transform in calcium sparks image de-noising when excitation contraction cou-pling(ECC) was studied and researched by scanning confocal microscopy,in order to elucidate the mechanism of ECC.But due to the noise exist in most calcium sparks image,it is hardly to recognize and analyze the useful information of calcium sparks.So how to increase the signal-to-noise rate is very important in calcium spark studying.Methods By using the self-developed software based on multi-scale wavelet transform to remove the noise exists in the calcium sparks images.Results The experiments shows that the method can be increased the signal-to-noise rate in about 80 calcium sparks images.Con-clusion The new method for calcium spark expression and spatial and temporal analysis is provided.

Information literacy is the essential literacy in medical education and training of information analysis ability is the difficulty in information literacy education .Described in this paper is the trial to help postgraduates and long-term schooling undergraduates select their study orientation and topics in ChinaMedical University , including their course targets, contents and teaching outcomes.

Objective To investigate the expression and importance of basic fibroblast growth factor(bFGF)in renal tissue of doxorubicin-induced nephropathy rats,and discuss its possible role in minimal change nephrotic syndrome(MCNS).Methods Fifty male Wistar rats were enrolled in this study.They were randomly divided into normal group(n=10)and nephropathic group(n=40).The nephropathic group was established by a single intraperitoneal injection of doxorubicin 5 mg/kg.The rats of nephropathic group were killed on the 3rd,7th,14th and 28th day and 24 h urinary protein of all rats was measured.Normal group rats were killed on the 28th day.bFGF level in renal tissue was determined with immunohistochemistry assays,and was quantitatively analyzed with color image analysis system.The SPSS 10.0 software was used to obtain t-test of various groups and correlation analysis of 2 variables.Results 1.The 24 h urinary protein of nephropathy rats increased gradually on the 7th,14th and 28th day,and it was greatly higher than that in control group(Pa

Objective To discuss the correlation between peak intensity (PI) of contrast enhanced ultrasound and injury severity after liver ischemia reperfusion.Methods Pringle's maneuver was used to establish the liver 15-min ischemia model.Contrast enhanced ultrasound was used to observe the hepatic perfusion after reperfusion of 1 h,6 h and 24 h,and the PI was recorded.The correlation analysis on PI with alanine aminotransferase (ALT),aspartate aminotransferase (AST) and pathologic changes (Suzuki standard) was done.Results PI decreased after 1 h,6 h,and 24 h of liver reperfusion,most significantly at 6 h.ALT reached the peak after 1 h of reperfusion and then decreased after 6 h and 24 h.AST increased after 6 h of reperfusion and decreased after 24 h.After 1 h of reperfusion,mild injury appeared in pathology and the injury became severe after 6 h,then it got moderate-to-severe after 24 h of reperfusion.PI was negatively correlated to ALT,AST and pathologic injury degree.Conclusion The PI relates to the degree of hepatic injury after liver ischemia/reperfusion.The more severe the injury is,the lower the PI.