1.Comparative study on intestinal metaplasia in Barrett's esophagus, cardia and gastric antrum
Guisheng LIU ; Jun GONG ; Jun ZHANG ; Ying CHANG ; Lei QIANG
Chinese Journal of Digestive Endoscopy 1996;0(04):-
Objective To compare the differences in mucin-histochemistry and etiology of Barrett's esophagus (BE) intestinal metaplasia (IM) , cardiac intestinal metaplasia (CIM) and gastric antrum intestinal metaplasia (GA-IM). Methods Alcian blue /periodic acid-schiff (AB/PAS) and high iron diamine / alcian blue (HID/AB) histochemical methods were used to classify IM in BE, cardia and gastric antrum, and IM were classified into three subtypes: complete small intestinal type (type Ⅰ ) , incomplete small intestinal type (type Ⅱ) and incomplete colonic type (type Ⅲ). Compared the prevalence of different subtypes of IM in above-mentioned sites, and investigated their relationships among the symptoms of gastroesophageal reflux disease (GERD) and Helicobacter pylori (Hp) infection. Results The prevalence of type Ⅲ IM in long-segment BE (LSBE) and short-segment BE (SSBE) is 75. 0% and 63. 3% respectively, it is significantly higher than that in CIM (23. 1% ) and GA-IM ( 17. 7% ) (P
2.Study on immunocyte proliferation and DNA damage induced by lead in mice.
Shu-ying DONG ; Chang-qing YING ; Ying WEN ; Ming-jun LU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(6):457-458
Animals
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Cell Proliferation
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drug effects
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Cells, Cultured
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Comet Assay
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DNA Damage
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Dose-Response Relationship, Drug
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Lead
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toxicity
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Lymphocytes
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drug effects
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Male
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Mice
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Mice, Inbred Strains
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Spleen
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cytology
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drug effects
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Thymus Gland
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cytology
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drug effects
3.The experimental study of the protective function of nabumetone on the injury of gastric mucosa caused by duodenogastric reflux
Rangeng SHI ; Jun GONG ; Lei DONG ; Ying CHANG
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
0.05 ). On the contrary, the COX-2 score in nabumetone group was obviously lower than that in the operation group (P 0.05 ). Conclusion Nabumetone has obvious therapeutic effects on bile-reflux gastritis caused by duodenogastric reflux. Nabumetone can significantly decrease the level of COX-2 in the gastric mucus membrane.
4.Expression and Antigenic Characterization of the Epitope-G1 of the Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris
Fu-ying, ZHENG ; Guo-zhen, LIN ; Chang-qing, QIU ; Kui-zhang, YUAN ; Jun-ying, SONG
Virologica Sinica 2007;22(5):347-352
The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1. Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS 115. The recombinant P. pastoris strains were selected by a G418 transformation screen and confirmed by PCR. After being induced with methanol, an expressed protein with 26 kDa molecular weight was obtained, which was much bigger than the predicted size (15.54 kDa). Deglycosylation analysis indicated the recombinant G1 was glycosylated. Western blot and ELISA tests, as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity. The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.
5.Effects of estrogen on neuron structure and expression of estrogen receptor in hypotha-lamus
Qing CHANG ; Da-Jun YING ; Chang-Xu SHI
Journal of Third Military Medical University 2001;23(3):318-320
Objective To explore the changes of ER-IR and the ultra structure in the medial preoptic area, arcuate nuclei of early-aged mice treated with estrogen. Methods Immunohistochemistry assay and electron microscopy were used in this study. Results ER-IR in the medical preoptic area and arcuate nuclei were greatly reduced after estrogen was given. The cell nuclei of neurons in these areas migrated towards the side, the nuclear membrane became folded, synapse became richer, and the number of synapse vesicle increased. Conclusion Estrogen can affect the neuron structure and function through the change of estrogen receptor expression in the medial preoptic area and arcuate nuclei of the hypothalamus cardiovascular center.
6.Expression of recombinant human acetylcholinesterase and its application in screening its inhibitors.
Xiang-Jun WANG ; Huai-Xiu WU ; Shan-Shan YE ; Lan-Ying PAN ; Yong-Chang QIAN
Acta Pharmaceutica Sinica 2014;49(1):50-54
This study is designed to obtain recombinant human acetylcholinesterase (rhAChE) and apply it in screening acetylcholinesterase inhibitors. The rhAChE was overexpressed in HEK293 cells transfected by plasmid of pCMV-AChE with the cationic liposome and rhAChE was found to be secreted into cell culture medium. AChE activity was assayed according to modified Ellman method to obtain kinetic parameters. IC so50 values for donepezil compounds of rhAChE were calculated to determine their activities of inhibition. The results showed that Km value was 151.9 micromol.L-1 donepezil inhibited rhAChE in a mixed competitive-noncompetitive way (Ki= 16.03 nmol.L-1, Ki = 18.36 nmol.L-1) and that most new compounds tested exhibited high activities of inhibition on rhAChE. The study suggests that rhAChE is available to be applied in screening AChE inhibitors in vitro.
Acetylcholinesterase
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genetics
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metabolism
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Cholinesterase Inhibitors
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analysis
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pharmacology
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HEK293 Cells
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Humans
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Indans
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analysis
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pharmacology
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Inhibitory Concentration 50
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Kinetics
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Piperidines
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analysis
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pharmacology
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Plasmids
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Recombinant Proteins
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genetics
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metabolism
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Transfection
7.The value of different renal size indicators in early screening of subclinical diabetic nephropathy with hyperfiltration
Ying WANG ; Jun LU ; Juhong YANG ; Junya JIA ; Chunyan SHAN ; Miaoyan ZHENG ; Baocheng CHANG ; Liming CHEN
Chinese Journal of Endocrinology and Metabolism 2012;(11):916-918
In subclinical diabetic nephropathy with glomerular hyperfiltration,the renal size parameters are increased significantly,and this change sets in as early as before the appearance of microalbuminuria.The average kidney length discriminator value for glomerular hyperfiltration by receiver operating characteristic (ROC) curve analysis is 10.53 cm,with the best sensitivity,higher specificity and total coincidence rate,and can be a clinical indicator for screening early diabetic nephropathy with glomerular hyperfiltration.
8.Nitric Oxide Regulated Expression of ?-Aminobutyric Acid B Recepto r Subunits during Recurrent Febrile Seizures
ying, HAN ; jiong, QIN ; ding-fang, BU ; zhi-xian, YANG ; xing-zhi, CHANG ; jun-bao, DU
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To explore the effect of nitric oxide (NO) on ?-aminobutyric acid B receptor (GABA_BR) subunits during recurrent febrile seizures (FS).Methods Twenty-four Sprague-Dawley rats aged 21 days were randomly divided into 4 groups: control group (37.0 ℃ water,n=8), FS group (45.2 ℃ water,n=8), FS + SNP group (45.2 ℃ water,n=8), FS+L-NMMA group (45.2 ℃ water,n=8). FS rats were induced 10 times in a warm-water bath, once every 2 days. The plasma level of NO was detected by the spectrophotometer. The expressions of GABA_BR subunit mRNA and c-fos gene were examined by in situ hybridization. The expressions of GABA_BR subunit and Fos protein were observed by immunohistochemistry. Results The plasma level of NO increased in FS + SNP group while decreased in FS+L-NMMA group compared with that in FS group. The expressions of GABA_BR_2 were down-regulated in FS+SNP group, while GABA_BR_1 hardly changed compared with those in FS group. In FS+L-NMMA group, both the expression of GABA_BR_2 and GABA_BR_1 up regulated compared with those in FS group. The expressions of c-fos gene and Fos protein were significantly enhanced after recurrent FS. SNP elevated the expressions of c-fos gene and Fos protein, while L-NMMA down regulated the expressions of them.Conclusion NO may play a regulatory role through modulating GABA_BR function in the pathogenesis of recurrent FS.
9.Influence of Gamma-Aminobutyric Acid B Receptor on Expression of Carbon Monoxide/ Heme Oxygenase System during Recurrent Febrile Seizures
ying, HAN ; jiong, QIN ; ding-fang, BU ; zhi-xian, YANG ; xing-zhi, CHANG ; jun-bao, DU
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To explore the influence of ?-aminobutyric acid B receptor(GABA_BR)on carbon monoxide (CO)/heme oxygenase(HO-1)system during recurrent febrile seizures (FS).Methods Sprague-Dawley rats aged 21 days were randomly divi- ded into 4 groups:control group and FS group,FS+baclofen group,FS+phaclofen group.FS in rats were induced 10 times in a bath of warm water, once every 2 days.The plasma level of CO was detected by the dual wave lengh spectrophotometer;the expressions of GABA_BR and HO-1 mRNA were examined by insitu hybridization;the expressions of GABA_BR and HO-1 protein were observed by immunohistochemistry.Results The plasma level of CO increased in FS+baclofen group,while decreased in FS+phaclofen group compared with FS group.The expressions of GABA_BR and HO-1 upregulated in FS+baclofen group,while decreased in FS+phaclofen group compared with FS group.There were significant difference (All P
10.Endoplasmic reticulum stress mediates lipopolysaccharide-induced apoptosis in rat hepatocyte.
Ying-Lei JI ; Jun YAN ; Yan-Sha WANG ; Yi-Chang LIU ; Zhen-Yong GU
Journal of Forensic Medicine 2014;30(1):13-18
OBJECTIVE:
To investigate the role of endoplasmic reticulum stress (ERS) in lipopolysaccharide (LPS)-induced hepatocyte apoptosis.
METHODS:
Cells of the rat hepatocyte line BRL were cultured. The hepatocytes were treated with LPS, ERS inducer thapsigargin (TG), and ERS inhibitor 4-phenylbutyric acid (4-PBA), respectively or in their different combination. The cell viability was measured by MTT assay. The cyto-nuclear morphological changes of apoptosis cells were detected by the fluorescent dye Hoechst 33258. The apoptosis rate was assessed by flow cytometry with Annexin V-FITC/PI double-staining. Expressions of GRP78 as ERS marker protein, CHOP, caspase-12 and cleaved-caspase-3 as ERS related protein were detected by Western blotting.
RESULTS:
LPS could cause a decrease in cell viability and an increase in apoptosis rate in a dose- and time-dependent manner. The expression of GRP78, CHOP, caspase-12 and cleaved-caspase-3 proteins were significantly increased with LPS treatment. TG led to a marked decrease in cell viability and an increase in apoptosis rate, which aggravated the hepatocyte injury induced by LPS; whereas 4-PBA alleviated LPS-induced apoptosis.
CONCLUSION
ERS mediates LPS-induced hepatocyte injuries, indicating that ERS may play a vital role in the pathogenesis of LPS-induced hepatocyte injuries.
Animals
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Apoptosis
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Caspase 3
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Cell Survival
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Endoplasmic Reticulum Chaperone BiP
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Endoplasmic Reticulum Stress
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Heat-Shock Proteins
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Hepatocytes
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Lipopolysaccharides
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Phenylbutyrates
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Rats