1.Comparative study on intestinal metaplasia in Barrett's esophagus, cardia and gastric antrum
Guisheng LIU ; Jun GONG ; Jun ZHANG ; Ying CHANG ; Lei QIANG
Chinese Journal of Digestive Endoscopy 1996;0(04):-
Objective To compare the differences in mucin-histochemistry and etiology of Barrett's esophagus (BE) intestinal metaplasia (IM) , cardiac intestinal metaplasia (CIM) and gastric antrum intestinal metaplasia (GA-IM). Methods Alcian blue /periodic acid-schiff (AB/PAS) and high iron diamine / alcian blue (HID/AB) histochemical methods were used to classify IM in BE, cardia and gastric antrum, and IM were classified into three subtypes: complete small intestinal type (type Ⅰ ) , incomplete small intestinal type (type Ⅱ) and incomplete colonic type (type Ⅲ). Compared the prevalence of different subtypes of IM in above-mentioned sites, and investigated their relationships among the symptoms of gastroesophageal reflux disease (GERD) and Helicobacter pylori (Hp) infection. Results The prevalence of type Ⅲ IM in long-segment BE (LSBE) and short-segment BE (SSBE) is 75. 0% and 63. 3% respectively, it is significantly higher than that in CIM (23. 1% ) and GA-IM ( 17. 7% ) (P
2.Study on immunocyte proliferation and DNA damage induced by lead in mice.
Shu-ying DONG ; Chang-qing YING ; Ying WEN ; Ming-jun LU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(6):457-458
Animals
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Cell Proliferation
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drug effects
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Cells, Cultured
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Comet Assay
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DNA Damage
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Dose-Response Relationship, Drug
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Lead
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toxicity
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Lymphocytes
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drug effects
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Male
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Mice
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Mice, Inbred Strains
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Spleen
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cytology
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drug effects
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Thymus Gland
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cytology
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drug effects
3.The experimental study of the protective function of nabumetone on the injury of gastric mucosa caused by duodenogastric reflux
Rangeng SHI ; Jun GONG ; Lei DONG ; Ying CHANG
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
0.05 ). On the contrary, the COX-2 score in nabumetone group was obviously lower than that in the operation group (P 0.05 ). Conclusion Nabumetone has obvious therapeutic effects on bile-reflux gastritis caused by duodenogastric reflux. Nabumetone can significantly decrease the level of COX-2 in the gastric mucus membrane.
4.Expression and Antigenic Characterization of the Epitope-G1 of the Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris
Fu-ying, ZHENG ; Guo-zhen, LIN ; Chang-qing, QIU ; Kui-zhang, YUAN ; Jun-ying, SONG
Virologica Sinica 2007;22(5):347-352
The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1. Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS 115. The recombinant P. pastoris strains were selected by a G418 transformation screen and confirmed by PCR. After being induced with methanol, an expressed protein with 26 kDa molecular weight was obtained, which was much bigger than the predicted size (15.54 kDa). Deglycosylation analysis indicated the recombinant G1 was glycosylated. Western blot and ELISA tests, as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity. The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.
5.Effects of estrogen on neuron structure and expression of estrogen receptor in hypotha-lamus
Qing CHANG ; Da-Jun YING ; Chang-Xu SHI
Journal of Third Military Medical University 2001;23(3):318-320
Objective To explore the changes of ER-IR and the ultra structure in the medial preoptic area, arcuate nuclei of early-aged mice treated with estrogen. Methods Immunohistochemistry assay and electron microscopy were used in this study. Results ER-IR in the medical preoptic area and arcuate nuclei were greatly reduced after estrogen was given. The cell nuclei of neurons in these areas migrated towards the side, the nuclear membrane became folded, synapse became richer, and the number of synapse vesicle increased. Conclusion Estrogen can affect the neuron structure and function through the change of estrogen receptor expression in the medial preoptic area and arcuate nuclei of the hypothalamus cardiovascular center.
6.Expression of recombinant human acetylcholinesterase and its application in screening its inhibitors.
Xiang-Jun WANG ; Huai-Xiu WU ; Shan-Shan YE ; Lan-Ying PAN ; Yong-Chang QIAN
Acta Pharmaceutica Sinica 2014;49(1):50-54
This study is designed to obtain recombinant human acetylcholinesterase (rhAChE) and apply it in screening acetylcholinesterase inhibitors. The rhAChE was overexpressed in HEK293 cells transfected by plasmid of pCMV-AChE with the cationic liposome and rhAChE was found to be secreted into cell culture medium. AChE activity was assayed according to modified Ellman method to obtain kinetic parameters. IC so50 values for donepezil compounds of rhAChE were calculated to determine their activities of inhibition. The results showed that Km value was 151.9 micromol.L-1 donepezil inhibited rhAChE in a mixed competitive-noncompetitive way (Ki= 16.03 nmol.L-1, Ki = 18.36 nmol.L-1) and that most new compounds tested exhibited high activities of inhibition on rhAChE. The study suggests that rhAChE is available to be applied in screening AChE inhibitors in vitro.
Acetylcholinesterase
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genetics
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metabolism
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Cholinesterase Inhibitors
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analysis
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pharmacology
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HEK293 Cells
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Humans
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Indans
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analysis
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pharmacology
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Inhibitory Concentration 50
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Kinetics
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Piperidines
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analysis
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pharmacology
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Plasmids
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Recombinant Proteins
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genetics
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metabolism
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Transfection
7.The value of different renal size indicators in early screening of subclinical diabetic nephropathy with hyperfiltration
Ying WANG ; Jun LU ; Juhong YANG ; Junya JIA ; Chunyan SHAN ; Miaoyan ZHENG ; Baocheng CHANG ; Liming CHEN
Chinese Journal of Endocrinology and Metabolism 2012;(11):916-918
In subclinical diabetic nephropathy with glomerular hyperfiltration,the renal size parameters are increased significantly,and this change sets in as early as before the appearance of microalbuminuria.The average kidney length discriminator value for glomerular hyperfiltration by receiver operating characteristic (ROC) curve analysis is 10.53 cm,with the best sensitivity,higher specificity and total coincidence rate,and can be a clinical indicator for screening early diabetic nephropathy with glomerular hyperfiltration.
9.Study of the antiulcer effect of pantoprazole
Ying-Xin WANG ; Xiao-Qiang LI ; Guang-Wu WU ; Shan-Jun QIAN ; Hong LEI ; Chang CAO ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim To evaluate the effects of pantoprazole on various experimental acute ulcer inrats and mice. Methods The model of a gastric ulcer of rats or mice was caused bystree- induced ulcer and ligatel pylurus-induced ulcer. Results & Conclusions At adose of 5, 10, 20 mg? kg-1 of Pantoprazole can markedly decrease the ulcer index ofstree-induced ulcer. Pantoprazole(4, 8, 16 mg? kg -1 ) significantly decrease the areaof ligated pylorus-induced gastric ulcer. It was also found that pantoprazole caninhibit the output of basic gastric acid.
10.Detection of?-catenin and c-myc expression in gastric adenocarcinoma by tissue microarray and its clinical significance
Wei-Chang CHEN ; Dong-Tao SHI ; Heng-Jun GAO ; Baofeng ZHANG ; Ying HU ;
Chinese Journal of Digestion 2001;0(12):-
Objective To study the expression and clinical significance of?-catenin and c-myc in gastric adenocarcinoma and corresponding para cancer gastric mueosa.Methods Two hundreds and ninety seven samples were collected from 102 cases of gastric adenocarcinoma,including 102 cancerous tissues and 195 para-cancerous tissues.Each sample was made into 282,156 and 156 dots tissue microarrays.Expressions of?-catenin and c-myc proteins were detected by immunohistochemical stain- ing.Results The expressions of?-catenin and c-mye were increased gradually in the process of gastric carcinogenesis.The rates of?-catenin and c myc expression were higher in carcinoma than that in intesti- nal metaplasia( P<0.05,P<0.001).The aberrant expression of?-catenin was closely related to the depth of invasion(P<0.05),and the expression of c-myc was related to histological grade( P<0.05). The aberrant expression of?-catenin was significantly correlated with the expression of c-myc in gastric adenocarcinoma(P<0.05).Conclusions The aberrant expression of?-catenin may activate the expres sion of target gene c-myc,which plays a pivotal role in the carcinogenesis and progression of gastric adenocarcinoma.