Objective To prepare mouse anti-human monoclonal antibodies against B7-H6 and to identify their biological characteristics. Methods The B7-H6 gene was cloned by RT-PCR from a human lung adenocarcinoma cell line ( A549 ) and then subcloned into the eukaryote expression vector pCMV3 to construct the recombinant vector pCMV3-B7-H6. The recombinant vector pCMV3-B7-H6 that was verified with enzyme digestion and gene sequencing was transfected into NIH/3T3 cells by electroporation. BALB/c mice were immunized with the successfully transfected cells named 2H8 through intraperitoneal injection. The monoclonal antibodies against human B7-H6 with the advantages of high affinity and specificity were pre-pared by using hybridoma technology. Western blot assay and flow cytometry analysis were used to identify the specificity of prepared monoclonal antibodies. Results The recombinant eukaryotic expression vector encoding B7-H6 was successfully constructed. Two hybridoma clones that stably secreted monoclonal anti-bodies against B7-H6 were screened out by using flow cytometry analysis and the monoclonal antibodies se-creted by them were belonged to IgG2a isotype. Specific reactions between B7-H6 and the secreted mono-clonal antibodies were confirmed by Western blot assay and flow cytometry analysis. Conclusion The mon-oclonal antibodies which recognized B7-H6 specifically were prepared successfully.

Adolescent ; Catheter Ablation ; methods ; Humans ; Male ; Pre-Excitation, Mahaim-Type ; Tachycardia, Atrioventricular Nodal Reentry ; surgery

To analyze maternal and neonatal complications among late preterm birth cases and to investigate risk factors of late preterm birth. Methods This was a retrospective analysis of 258 late preterm cases (late preterm group) born in Peking University First Hospital from January 1, 2009 to December 31, 2010. Maternal comorbidity and complications, delivery modes, and neonatal complications of these 258 late preterm infants were compared with 308 term cases (term group) during the same period. Statistical analysis was performed usingχ2 test, Fisher's exact probability test, t test and logistic regression. Results In Peking University First Hospital, late preterm births accounted for 3.9%(258/6 695) of live births and 60.1%(258/429) of preterm births. The incidence of the following maternal complications among the late preterm group was higher than that among term group(all P<0.05): severe pre-eclampsia [7.4%(19/258) vs 1.0%(3/308), χ2=15.35]; preterm rupture of membrane [42.6%(110/258) vs 15.3%(47/308), χ2=52.49];cervical insufficiency [1.9%(5/258) vs 0.0%(0/308), Fisher's exact test];placenta previa[3.5%(9/258) vs 0.6%(2/308), Fisher's exact test] and placental abruption [2.7%(7/258) vs 0.3%(1/308), Fisher's exact test]. Severe pre-eclampsia was the major risk factor leading to late preterm birth. The incidence of the following neonatal complications among the late preterm group was higher than that among term group (all P<0.05):respiratory distress syndrome (NRDS) [11.6%(30/258) vs 1.6%(5/308), χ2=24.22]; hyperbilirubinemia [64.3%(166/258) vs 39.6%(122/308),χ2=34.36];electrolyte disturbance [12.8%(33/258) vs 1.6(95/308),χ2=27.96];hypothermia [7.0%(18/258) vs 2.9%(9/308),χ2=5.08];infectious pneumonia[13.6%(35/258) vs 3.2%(10/308), χ2=20.43]; leukoencephalopathy [3.1%(8/258) vs 0.3%(1/308), χ2=5.25]; low body temperature [18.6%(48/258) vs 3.6%(11/308),χ2=33.98] and neonatal asphyxia [6.2%(16/258) vs 1.0%(3/308),χ2=11.86]. The incidence of the following neonatal complications among late preterm infants born at<35 weeks gestation was higher than that among late preterm infants born at≥35 weeks gestation (all P<0.05):NRDS [30.4%(14/46) vs 7.5%(16/212) ,χ2=19.26];hyperbilirubinemia [91.3%(42/46) vs 58.5%(124/212), χ2=17.74]; electrolyte disturbance [21.7%(10/46) vs 10.8%(23/212), χ2=4.02]; intracranial hemorrhage [8.7%(4/46) vs 1.9%(4/212),χ2=3.88];leukoencephalopathy [10.9%(5/46) vs 1.4%(3/212),χ2=8.32] and neonatal asphyxia [15.2%(7/46) vs 4.2%(9/212), χ2=6.05]. Conclusions Severe pre-eclampsia is the major risk factor leading to late preterm birth. The incidence of complications among late preterm infants is higher than that among term infants. If a pregnancy has to be terminated because of maternal disorders, the pregnancy period should be extended to 35 weeks if it permits.

Objective:To study the influence of laboratory mental stress on plasma levels of angiotensin II (Ang II), aldosterone(ALD)and atrial natriuretic peptide (ANP) in individuals with different salt sensitivity Method:48 healthy male undergraduates were divided into salt sensitive group (SS group, n=14) and non-salt-sensitive group (NSS group, n=34) Their blood pressure, plasma levels of Ang II, ALD and ANP were measured before and after mental arithemtic test Result:Blood pressure, plasma levels of Ang II and ALD increased significantly in both groups, with SS group having greater increase Plasma level of ANP had no change after the test Conclusion:Laboratory mental stress increases the plasma levels of Ang II and ALD in male students Salt-sensitivity can magnify this effect

Objective To develop a kind of mobile Bluetooth temperature sensor with removable measurement and wireless transmission.Methods The device was designed with technology of high precision sensor and newly developed Bluetooth base on MCU MSP430 as control unit which could apply in real time measurement,data storage,transmission and emergency alarm.Results The problem that at the scene of many cables in clinical care of the devise was resolved.Any devices with Bluetooth sensor can receive temperature data.Conclusion The traditional work mode is changed by the Bluetooth device and reduces the workload of nursing staff,which provides a new solution for integration of Bluetooth and clinic care technology.

Antineutrophil cytoplasmic antibody-associated vasculitis (AAV) refers to the groups of potentially life-threatening autoimmune disease.Antithyroid drug is one of the causes.Usually the involved organs are skin,kidney,and (or) lung.Early diagnosis and treatment of AAV is essential.Timely cessation of antithyroid drugs is the first step.If necessary,glucocorticoids and (or) immunosuppressive agents should be used to delay the progression of the disease.

Objective To explore the early clinical characteristics and influencing factors in children with autism spectrum disorders(ASD).Methods From January 2005 to December 2014,193 children with ASD were collec-ted by continuous grouping method from Children's Rehabilitation Training Center in Xi'an.According to the 1∶1 matched case-control study requirements,and the other 193 children from kindergartens and primary schools in the urban areas of Xi'an were collected as healthy control group from March 1 to July 1,2016.The age of children in the case group was(40.78±14.86)months and the age of the healthy control group was(40.61±14.40)months.There were 167 boys and 26 girls in 2 groups and the ratio of boys to girls was 6.42∶1.00.The general status questionnaires,medical history questionnaire,diagnostic chart,Autism Behavior Checklist(ABC)and Family Environment Scale of Chinese version(FES-CV)were completed by parents between 2 groups.Childhood Autism Rating Scale(CARS)was completed by doctors in the case group.By using Excel software,the original questionnaires were completed in 2 entries by 2 persons to set up the database.All data were analyzed by SPSS 17.0 statistical software and conditional Logistic regression was used for multivariate analysis.Results Seventy point eight percent(137/193 cases)of children with ASD had been found abnormal under 2 years old or at 2 years old,and 54.9％(106/193 cases)had been diagnosed under 3 years old or at 3 years old.The average delay from the discovery to the diagnosis was 17 months.The initial abnormalities appea-rances were mainly manifested as no response to calling in 153 cases(79.3％),very little active contact with others in 141 cases(73.1％),silent or less use of oral language in 137 cases(71％),avoiding contact with the eyes of others or lack of facial expressions in 121 cases(62.7％).Their signs were easy to be misdiagnosed as mental retardation and language retardation.Children in the case group began to walk alone at the age of 8 months to 3 years old,and only 62.2％(120/193 cases)of them could walk alone at the age of 18 months or before.The age of conscious speech was at 8 months to 4 years and 4 months,and only 39.4％(76/193 cases)of the ASD children could speak at the age of 18 months or before.The total scores of the ABC scale of the case group were(56.520±22.140)scores and the sub-scales and total scores were significantly higher than those of the healthy control group,and the difference was statistically significant(t=16.845,27.390,16.527,26.320,23.371,32.206,all P<0.001).The positive consistent rate of ABC and clinical diagnosis was 56.5％.The total scores of CARS in the case group was(36.4±8.6)scores,and the positive consistent rate of CARS and clinical diagnosis was 78.8％.There was a statistical significance between the 2 groups in parental education,mother's occupation,family history(x2=29.670,44.593,15.439,6.095,all P<0.05),and there were statistical significance in the main caregivers,family harmony and family income(x2=19.006,7.129,109.027,all P<0.05).There was no statistical significance between the 2 dimensions of independence and achievement orientation between the 2 groups(t=-1.559,-0.139,P=0.120,0.890).The case group in the family cohesion,expressiveness,intellectual-cultural orientation,active-recreational orientation,moral-religious emphasis,organization and control of the 7 dimension scores were significantly lower than those in the healthy control group,and the differences were statistically significant(t=-7.683,-5.734,-8.762,-14.109,-2.026,-4.530,-2.464,all P<0.05).In the case group,the scores of the conflict dimension were higher than those of the healthy control group,and the difference was statistically significant(t=4.925,P<0.001).There was a statistical significance between the 2 groups in gestational age and birth hypoxia(x2=6.898,27.180,all P<0.05).According to multivariate analysis of Logistic regression,people other than parents serving as the primary support,anoxia of newborn,mother of non professional and technical personnel and lower scores of family active-recreational orientation might be the risk factors of ASD,family per capita income of 3 000 Yuan RMB or more monthly,mother education level of high school and above,and lower scores of family conflict might be the protective factors for ASD.Conclusions Clinical features of most ASD children can be easily identified under 2 years old,but if the diagnosis is delayed,the related intervention is late,so importance should be attached to early diagnosis.Mother's occupation and education level,family economic status,family environment,their supervisors,and anoxia of newborn may be the effective entry points in the prevention and treatment of ASD.

Objective To summarize the experience of nursing patients undergoing NovaSure endometrial ablation. Methods Two hundred and eleven patients with abnormal uterine bleeding claiming no need of giving birth,who were hospitalized in our hospital during August 2011 to April 2013,were managed with NovaSure endometrial ablation and nursing care was performed.Follow-ups were conducted for investigating the curative effects.Results All the patients lived through the operations,their life signs were stable.As for the adverse effects,124 of them had mild abdominal pains,7 medium-level or severe hypogastralgia,1 dizziness and nausea,10 uterial hematocele,and 1 cystitis.All of them got recovered after treatment.One-month follow-up after hospitalization showed a success rate of 98.0%.Conclusions NovaSure is a new approach to abnormal uterine bleeding.It is advantageous for its simplicity,shortness in operation duration,less trauma to patients and good effects.Meanwhile,preoperative mental care and cooperative care during operation may facilitate their recoveries.

OBJECTIVE: To explore the effects of Pollen Typhae total flavones (PTF) on the mRNA expressions of peroxisome proliferator-activated receptors (PPARs) alpha,gamma, beta/delta so as to analyze its possible mechanism in improving the insulin sensitivity of 3T3-L1 adipocytes. METHODS: Adipocytes were treated with PTF, and expressions of PPARalpha, PPARgamma, PPARbeta/delta mRNAs relating to the adipocyte glucose and lipid metabolism were determined by reverse transcription polymerase chain reaction. RESULTS: PTF obviously up-regulated the expressions of PPARalpha and PPARgamma mRNAs, all showing significant differences as compared with those in the normal control group (P<0.01). CONCLUSION: With its function as an insulin sensitizer, PTF may enhance the PPARalpha and PPARgamma mRNA expressions in 3T3-L1 adipocytes.

Objective To investigate the risk factors of sexual dysfunction in male patients with obstructive sleep apnea and hypoventilation syndrome (OSAHS).Methods One hundred and twenty-one OSAHS male patients aged 22-50 years were stratified into two groups based on scores on the rating scales of international index of erectile function-5 (IIEF-5).Patients' characteristics and polysomnography data of the sexual dysfunction group (n =56) were compared with that of the normal group (n =65).Continuous positive airway pressure(CPAP) treatment was carried out in some patients with abnormal sexual function.Results There were significant differences between the two groups in body mass index (BMI),history of hypertensive disease and diabetes mellitus,the rise of morning blood pressure,respiratory disturbance index (RDI),the longest apnea time(Tmax),the lowest oxygen saturation(LSpO2),percentage of recording time with oxygen saturation of 90％ or less(T90％),slow wave sleep(S1 + S2) over total sleep time(TST),rapid wave sleep (S3 + S4) time over TST,and rapid eye movement (REM) sleep time over TST in bivariate analysis (P ＜ 0.05).There were statistical significance of the history of diabetes mellitus,slow wave sleep (S1 + S2) over TST,RDI,and LSpO2shown in Logistics regression analysis,and ORs were 2.10 (1.17-5.01),3.52 (1.33-5.97),1.45 (1.08-2.95),and 0.86 (0.67-0.93) respectively (P ＜ 0.05).Moreover,20 OSAHS patients with sexual dysfunction were revaluated after the CPAP treatment in two months,and 14 patients returned to the normal scores.Conclusion The history of diabetes mellitus,slow wave sleep (S1 + S2) over TST,RDI,and LSpO2 were independent risk factors for sexual dysfunction in male patients with OSAHS,and treatment with CPAP could significantly improve sexual function in male patients.