The HLA DQA1 genes of 40 IDDM patients (including 8 cases with onset of diabetes before 14 years of age, 19 between 15-30 years and 13 after 31 years) and 51 healthy controls were studied by using for allele specific oligonucleotide probes. All of our research subjects are of Southern Chinese origin. The results showed that the HLA DQA1 52Arg(+) associated IDDM susceptibility is significantly higher in the group younger than 14 years of age (P

Objective To search for evidence of diagnosis of pulmonary embolism for guiding clinical practice.Methods We searched PubMed(1970~2005-10)and CBM(1992~2005-10)in Oct.2005 to identify system reviews(SR)and cross-sectional study(CSS)about the diagnosis of pulmonary embolism.Results Sixteen SR and 9 CSS were identified in PubMed,none in CBM.Pulmonary arteriography was the gold standard in the diagnosis of pulmonary embolism.Our result showed that computed tomography,ventilation-perfusion scanning and doppler ultrasound were useful in the diagnosis of pulmonary embolism combind with clinical judgment.Multidetector CT might be another gold standard in the diagnosis of pulmonary embolism.A negative result on quantitative rapid ELISA of D-dimer could exclude the diagnosis of pulmonary embolism.Conclusion Diagnostic tests combind with clinical judgment are useful for diagnosing or excluding pulmonary embolism.

AIM:To evaluate the poor vision condition and analyze the myopia etiological factor in primary school He'xi district of Sanya.
METHODS: A cohort of 1 218 subjects were recruited randomly from grade 1~6 of two primary schools. Visual activity test, dioptroscopy and risk factor questionnaire were evaluated.
RESULTS: The prevalence of poor vision was 29. 55%. The incidence of myopia increased with grade, and was significantly higher in girls than that in boys. The outdoor activity time of myopic pupil ( 7. 25 ± 5. 35h/wk ) was significantly lower than emmetropic pupil (11. 86±6. 65h/wk) ( P < 0. 05 ). The usage of electronic product ( TV, computater and cell phone) time of myopic pupil (13. 36±8. 35h/wk) was significantly higher than emmetropic pupil (7. 75±5. 83h/wk) (P<0. 05).
CONCLUSION: The increase of myopia incidence is closely related with sparing outdoor activity and excess usage of electronic product.

We present an algorithm for numerical solution of transport equation in diffusive regimes, in which the transport equation is nearly singular and its solution becomes a solution of a diffusion equation. This algorithm, which is based on the Least-squares FEM in combination with a scaling transformation, presents a good approximation of a diffusion operator in diffusive regimes and guarantees an accurate discrete solution. The numerical experiments in 2D and 3D case are given, and the numerical results show that this algorithm is correct and efficient.

Trans-cinnamaldehyde, the main component of volatile oil from cassia twig or Cinnamomum cassia, which is a traditional Chinese herbal medicine. Trans-cinnamaldehyde is a kind olefine aldehyde of organic compounds and has many pharmacological properties, such as anti-inflammatory, anti-tumor, anti-bacterial, antidiabetic, anti-obesity, and neuroprotection etc. The compound has preventive and therapeutic effects on the nervous system, cardiovascular, cancer, diabetes and other diseases. Trans-cinnamaldehyde, as a preventive care of nature medicine, has great clinical and market potential. This paper gives a review about the pharmacological effects and mechanism of trans-cinnamaldehyde researched in the latest five years. We hope to provide some basic information for further research on trans-cinnamaldehyde.
Acrolein ; analogs & derivatives ; chemistry ; pharmacology ; Animals ; Cinnamomum aromaticum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans

AIM: To explore the effects of prostaglandi n I 2 (PGI 2) on mesenteric microcirculation and hemorheology during renal isch emia/reperfusion (IR) injury. METHODS: 36 rabbits were randomly distributed into the sham oper ated group (sham group), renal ischemia/reperfusion injury group (IR group) and PGI 2+IR group(PGI 2 group). IR group received clamping for 60 min follow ed by 120 min of reperfusion. A microcircular microscope image analysis system w as used to study the changes of mesenteric microcirculation and hemorheology at 60 min of ischemia and 120 min of reperfusion, respectively, while the blood sa mples were obtained for the measurement of hemorheological indexes. RESULTS: ① In IR group during the period of renal IR, the numb er of adhesive leukocytes and microthrombus, hemorrhage and hemorheological ind exes such as blood viscosity, plasma viscosity, blood reduction viscosity, he matocrit, erythrocyte aggregation index, erythrocyte sedimentation rate, eryt hrocyte sedimentation rate K and plasma fibrinogen were significantly higher, w hile microvascular diameters, blood flow velocity and erythrocyte deformation i ndex were significantly lower compared with sham group (P0.05). CONCLUSIONS: PGI 2 ameliorates the disturbance of mesenteric microcirculation and hemorheology caused by renal IR injury with the best effect at 10 ng?kg -1?min -1.

0.05) between two groups.Conclusion Two surgicel porcedures offer smilar results in the surgical treatment of primary spontaneous pneumothorax.Both techniques are equally safe,small traumatic,pain controllable,and there are little postoprerative complications and the relapse rate is very low.

Anesthesia ; methods ; Child ; Child, Preschool ; Female ; Foreign Bodies ; surgery ; Humans ; Infant ; Male ; Muscle Relaxants, Central ; therapeutic use ; Respiratory System

Objective To investigate the expression of Rap1 GTPase-activating protein 1 (Rap1GAP),matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9),and their relation with clinical patterns in colorectal carcinoma.Methods Immunohistochemistry was used to detect the expression of Rap1GAP,MMP-2 and MMP-9 in colorectal carcinoma,villous adenoma,tubular adenoma and normal colorectal tissue,and their relationship with clinicopathological parameters was analyzed.Results The positive rate of Rap1GAP expression was 30.4 ％ (14/46),77.8 ％ (14/18),69.6 ％ (16/23) and 95.2 ％ (20/21) in colorectal carcinoma,villous adenoma,tubular adenoma,and normal colorectal tissue,respectively (x2 =30.659,P=0.000).The figures were 71.7 ％ (33/46),55.6 ％ (10/18),52.2 ％ (12/16) and 9.5 ％ (2/21) for the positive rate of MMP-2 expression (x2 =22.459,P =0.000),as well as for 76.1％ (35/46),61.1％ (11/18),56.5 ％ (13/23) and 14.3 ％ (3/21) for the positive rate of MMP-9 expression,respectively (x2 =22.643,P =0.000).In patients with colorectal carcinoma,the expression of Rap1GAP was correlated with tumor differentiation (x2 =5.275,P =0.022),but not sex,age,or lymphatic metastasis (all P ＞ 0.05).The expression of MMP-2 and MMP-9 were correlated with lymphatic metastasis (x2 =6.661,P =0.010;x2 =8.475,P =0.040),but not sex,age or tumor differentiation(all P ＞ 0.05).There was a negative correlation between expression of Rap1GAP and MMP-2,MMP-9 in colorectal carcinoma,respectively (r =-0.424,P =0.003;r =-0.294,P =0.048),but no correlation between the expression of MMP-2 and MMP-9 (r =0.101,P =0.505).Conclusions Rap1GAP,MMP-2 and MMP-9 play important roles in the malignant biological behavior of colorectal carcinoma,and the expression of Rap1GAP is negatively correlated with MMP-2 and MMP-9.The interactions among the three affect the occurrence and development of colorectal carcinoma.

BACKGROUND:The cel sheet technology that is applied with the absence of scaffolds and enzymatic digestion can effectively repair tissue defects and improve organ function, by stimulating the secretion of extracelular matrix to form a dense membrane tissue.
OBJECTIVE: To review the recent progress in cel sheet technology used in tissue engineering, thereby providing a new idea for relevant basic and clinical research.
METHODS:The first author retrieved CNKI database, Wanfang database and PubMed with the keywords of “cel sheet, tissue engineering” in Chinese and English, respectively. Literature retrieval period was from January 2010 to July 2015.
RESULTS AND CONCLUSION:Cel sheet technology combined with scaffold materials can be used for reconstruction and repair of tissues and organs. With the emerging of new technologies, multi-layer cel sheets are stratified to form a three-dimensional tissue for repair of soft tissues and organs. Compared with the monolayer cel sheet, the three-dimensional cel sheet that is laminated by same or different cel sheets has stronger regenerative ability and can be used to construct the ideal target tissue modelin vitro. Cel sheet technology combined with scaffolds can improve the mechanical strength of the composite and reduce cel loss, which has made great progress in the repair of tooth, bone and cartilage tissue. Currently, the cel sheet technology is at the laboratory stage, and little is reported on its clinical applications. We look forward to more innovative technologies that can be integrated into the cel sheet technology.