Objective To observe the effects of hyperbaric oxygen (HO) and free radical antagonists (FRA) on liver fibrosis (LF) in chronic hepatitis (CH) patients.Methods Seventy-two CH patients were ran- domly divided into a HO-FRA group (36 cases) and a control group (36 cases).The patients in the control group received routine treatment,while patients in the HO-FRA group were treated with hyperbaric oxygen,vitamin E and vitamin C.All patients' livers were examined ultrasonically,and liver function and blood fibrosis indices were meas- ured before and after the treatment.Results Compared with those in the control group,the concentrations of blood hyaluronic acid,laminin,precollagen and typeⅣcollagen were all significantly lower in the HO-FRA group.Ultra- sonic imaging showed that the main portal and the splenic vein diameter,and the length and thickness of the spleen had also significantly declined in the HO-FRA group after treatment.Conclusion HO and FRAs can nelp to stave off liver fibrosis as indicated by blood and ultrasonic fibrosis indices.

Actinin ; genetics ; Adolescent ; Adult ; Asian Continental Ancestry Group ; genetics ; Athletes ; Athletic Performance ; China ; Ethnic Groups ; genetics ; Female ; Humans ; Male ; Polymorphism, Genetic ; Young Adult

Objective To explore the clinical metabolic characteristics and the blood glucose variability in the insulin resistance polycystic ovary syndrome (PCOS) patients.Methods Totally 69 cases of PCOS patients were divided into two groups:PCOS with insulin resistance (PCOS-IR) group and PCOS without insulin-resistance (PCOS-NIR) group.Their clinical and metabolic characteristics were analyzed,and continuous glucose monitoring system (CGMS) was used to monitor the glucose concentration of subcutaneous interstitial fluid to reflect the blood glucose level.Mean blood glucose level (MBG) and its standard deviation (SDBG),mean amplitude of glycemic excursion (MAGE),peak level of postprandial plasma glucose as well as its peaking time during a 48-hour period CGMS were calculated.Results MBG,SDBG,large amplitude of glycemic excursions (LAGE),MAGE level in PCOS-IR group,were significantly higher than those in PCOS-NIR (P ＜ 0.05).Body mass index (BMI),triglycerides,glycated hemoglobin Alc (HbAlc),fasting blood glucose (FBG),2 hours glucose,free androgen index (FAI) level in PCOS-IR group were higher than in PCOS-NIR group (P ＜ 0.01).The difference of total cholesterol,low-density lipoprotein,mean of daily difference (MODD) level were not statistically significant (P ＞ 0.05).Conclusions PCOS-IR patients suffer from more serious metabolic disorder and blood glucose variability.

Objective To investigate the clinical spectrum of respiratory viruses in infants and young children with acute lower respiratory infection(ALRI) in Chongqing area from 2003-2007.And to assess the clinical diagnostic value of virus detection in nasopharyngeal secretions(NPS) and serum viral antibody detection for ALRI.Methods Cases of 2 529 specimens of NPS in hospitalized children with ALRI from Apr.2003 to Oct.2007 were taken for detecting 7 common respiratory virus antigens by immunofluorescence assay including respiratory syncytial virus (RSV),adenovirus(ADV),influenza A(IA),influenza B (IB),parainfluenza virus1-3 (PIV1,PIV2,PIV3).Fifty-five thousand eight hundred and eighty-seven samples were tested for ADV-IgM by ELISA.Among those,45 159 cases were further tested for RSV-IgM by ELISA.Results Respiratory virus pathogens were detected in 778 samples out of 2 529(30.76%) including RSV positive in 668 samples (85.86%),PIV3 positive in 75 samples (9.64%),IA positive in 22 samples (2.57%),ADV positive in 15 samples ( 1.93%),only 1 sample ( 0.13%) positive for both PIV1 and RSV. And the positive rate of RSV-IgM was 0.9%-15.2%,and the positive rate for ADV-IgM was about 0.6%-10.6%.RSV infection occured mainly in winter and spring.Conclusions Respiratory virus is the most common pathogen in children with ALRI during the survey period in Chongqing area,especially for RSV infection.The pattern of RSV circulation varied every year with seasonality.It is suggest that this year is peak one for RSV infection from the monthly positive results,especially in Feburary(50%) in 2007.But the infection rate of PIV3,IA,ADV and PIV1 are lower,particularly IB and PIV2 infection have not been seen for the last 5 years.It is fast and accurate to detect RSV antigen and suit to clinical diagnosis by using immunofluorescence assay than other antibody detection.

This paper analyzes the scientific research guidance, author's area & units distribution, coauthor rate and quotations in "Chinese Journal of Medical Instrumentation", and offers some suggestions for the Journal so as to play an important role in scientific research in the future.
Bibliometrics ; China ; Humans ; Journalism, Medical ; Multivariate Analysis ; Periodicals as Topic ; statistics & numerical data

Objective To evaluate the expression quantity of T lymphoma invasion and metastasis inducing factor 1(Tiara 1)in gastric cancer tissues,tissues around the cancer over 3 cm and tissues of gastric benign lesions,and to analyze the correlation between amount of Tiaml and gastric cancer biological behaviours.Methods Real-time fluorescence quantitive RT-PCR(FQ-RT-PCR)was used to detect the expression level of Tiaml mRNA in 66 cases of gastric cancer tissues,tissues around the cancer over 3 cm and the gastric tissues of 11 cases with benign lesion.Results Positive rate of Tiam1 mRNA expression in gastric cancer tissues,tissues around the cancer was 92.4%,19.7%,respectively,and only one case express Tiaml mRNA in 11 cases gastric benign lesion tissues.There was significant difference(P0.05),on the other hand,the expression quantity of Tiaml mRNA in gastric cancer was significantly correlated with metastasis into lymph node,the extent of invasion,differentiated degree of histologic type,and the stages of TNM(P

The purpose of this study was to investigate the existence and extent of cognitive impairment in adult diabetes mellitus (DM) patients with episodes of recurrent severe hypoglycemia,by using meta-analysis to synthesize data across studies.PubMed,EMBASE and Cochrane library search engines were used to identify studies on cognitive performance in DM patients with recurrent severe hypoglycemia.Random-effects meta-analysis was performed on seven eligible studies using an inverse-variance method.Effect sizes,which are the standardized differences between the experimental group and the control group,were calculated.Of the 853 studies,7 studies met the inclusion criteria.Compared with control subjects,the adult DM patients with episodes of recurrent severe hypoglycemia demonstrated a significantly lowered performance on memory in both types of DM patients,and poor performance of processing speed in type 2 DM patients.There was no significant difference between adult DM patients with and those without severe hypoglycemia in other cognitive domains such as general intelligence,executive function,processing speed and psychomotor efficiency.Our results seem to confirm the hypothesis that cognitive dysfunction is characterized by worse memory and processing speed in adult DM patients with a history of recurrent severe hypoglycemia,whereas general intelligence,executive function,and psychomotor efficiency are spared.

Adult ; Chronic Disease ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Occupational Diseases ; Radiation Injuries

BACKGROUND:There are many researches on the mouse skin morphology and development in embryonic period,but we have a poor understanding of the histomorphological evolution process of the skin and hair follicle after birth.OBJECTIVE:To explore the morphological changes and rules of the newborn mouse skin and hair follicle development.METHODS:The thickness of the back skin from the 1-12-day-old mice was analyzed using Image J software through conventional paraffin-embedded section and hematoxylin-eosin staining methods.The morphology of the hair follicle was observed under an optical microscope,and the number of hair follicles and vesicle-shaped structures were recorded.The development of hair follicles according to the developmental characteristics was divided into four periods,in comparison with Ralf Paus staging.RESULTS AND CONCLUSION:On the early stage of hair follicle formation (1-4 days),the newborn mice appeared with thinnest skin [(405.36±26.60) μm in thickness] on the day of birth,and there was no adipose tissue in hypodermis and few hair bulbs in epidermis.On day 2,the skin was thickened to (927.11±27.25) μm,with more dermal fibroblasts,and the horny cells from the stratum basale migrated to forming hair papilla.On days 3-4,the skin was thinned to (738.60±82.07) μm,increasing hair follicles and expanded hair bulbs were observed,with the dermis covered by dermal sheath.On the middle stage of hair follicle formation (5-7 days),the skin was thickened again on day 5,and became thickest [(2 369.57±34.06) μm in thickness] on day 7,there were increased number of hair follicles,hair bulbs and vesicle-shaped structures,the internal dermal sheath was extended into the epidermis to form hair roots,the dermal sheath was differentiated into external root sheath,and the internal root sheath extended deeply to form a closed germinal matrix.On the later stage of hair follicle formation (8-9 days),the skin thickness was decreased to (1 743.37±75.05) μm on day 8 and (1 987.07 ±18.65) μm on day 9;the hair papilla was surrounded by hair matrix;hair canal and hair shaft gradually formed,and the growing internal root sheath extended into the deep layer,forming more hair roots.On the maturation sage of hair follicle (10-12 days),the skin was thickened to (2 399.33±27.00) μm;the number of both hair follicles and vesicle-shaped structures was about 30;the hair shaft lengthened,reaching the muscle layer,which grew upward through the hair canal and further crossed the epidermis to form the hair that could be observed by the naked eye.These findings suggest that there is synchronical or non-synchronical correlation between the skin development and hair follicle formation at the neonatal mouse back,presenting with the ascendant curve.The development process of hair follicle is imbalanced and different,and staging of hair follicle formation is available for observing the differentiation progress of hair follicle.

Protein tyrosine phosphatase (PTP) 1B is a potential therapeutic target for type 2 diabetes. Phosphotyrosine (pTyr) mimetics still dominate the currently available PTP1B inhibitors. The phenoxyacetic acid moiety was taken as a pTyr mimetic herein and phenoxyacetic acid-based compounds 2a-2g and 3a-3c were designed. Among them, compounds 2a-2g exhibited potent inhibition against PTP1B, and compound 2g showed an IC₅₀ of 0.42 μmol·L^-1 against PTP1B. Compound 2f exhibited pharmacological profiles similar to that of rosiglitazone, and could improve the insulin sensitivity and the serum total cholesterol level. The results suggest that PTP1B inhibitors might be effective in treating type 2 diabetes as well as associated metabolic syndromes.