Objective To explore the characteristics of emotion recognition in children aged 7-14 years. Methods Nine hundred and sixty-one students aged 7-14 years were enrolled to rate the emotion dimensions of valence, arousal and dominance with the scale of Native Chinese Affective Picture System for Children. The relationship among scores of each emotion dimension was analysed, and the differences in age and gender of each emotion dimension were determined. Results For positive pictures (score of valence >6), the score of valence was positively related to that of arousal and dominate (r=0.56, r=0.40, P<0.01). For neutral pictures (score of valence 4-6), the score of valence was negatively related to that of arousal (r=-0.70, P<0.01) and positively related to that of dominance (r=0.69, P<0.01). For negative pictures (score of valence<4), the score of arousal was negatively related to that of dominance (r=-0.95, P<0.01). There were significant differences in scores of emotion dimensions between genders (P<0.05). There were significant differences in scores of arousal and dominance among children with different ages (P<0.01). Age was weakly negatively related to score of arousal (r=-0.18, P<0.01), and was weakly positively related to that of dominance (r=0.10, P<0.01). Conclusion There are significant differences in age and gender for emotion recognition in children, and the ratings are various among pictures with different characteristics, which needs to be considered in further researches.

Contrary to being an idle bystander,the surrounding tumor microenvironment or stroma,actively participates in,and contributes to tumor progression directly or indirectly.It is evident that tumor stromal cells exhibit specific tumor-suppressive and tumor-promoting abilities that serve to regulate neoplastic growth of the epithelium.Due to the mounting evidence demonstrating the far-reaching effects of stroma during the process of malignant transformation,the involvement of stroma in cancer progression has become an area of intense investigations in recent years.

Objective To explore the effect of chromosomal abnormality and polygenic inheritance factor in female children with short stature.Methods 1.Chromosome analysis:peripheral blood was drawn for 1 mL and cultured 72 h to analyze chromosome karyotype (Giemsa Banding ) of peripheral lymphocytes.2.Polygenic factor analysis:the children′s final height were estimated based on their parents average height,and analyzed the distribution characteristics of children′s final height and compared the estimate final height with the actual height.Results Eighty-three cases out of the 364 female children with short stature were chromosomal abnormality(22.80%).Among the 83 cases,the 45,XO and 46,X,i(Xq) occupied 70%.The distribution of children target height shifted left,and the target height of 76 cases was lower than 2 standard deviation (-2 s)and the consistency of target height and actual height reached 20.88%.The target height of 7 cases was lower than 2 standard deviation in those whose chromosome turned out to be abnormal,and the consistency of target height and actual height was 8.43%.Conclusions Chromosomal abnormality is one of the most important etiologic agents causing short stature in female children, and polygenic inheritance is another important etiologic agent.

Objective To investigate the efficacy of acupuncture and moxibustion plus modern medication for lower-limb deep venous thrombosis. Method Ninety patients with lower-limb deep venous thrombosis were allocated, using a random number table, to control and observation groups. The control group received anticoagulant, thrombolytic and volume expansion therapy and the observation group, acupuncture and moxibustion in addition.Hemorheological indicators, D-dimer concentration and lower-limb venous blood flow velocity were measured before and after treatment. Result There were statistically significant pre/post-treatment differences in whole blood viscosity and platelet aggregation rate in the control group of patients after treatment (P<0.05). There were statistically significant pre/post-treatment differences in whole blood viscosity, plasma viscosity, hematocrit, erythrocyte electrodeposition rate and platelet aggregation rate in the observation group of patients after treatment (P<0.05). After treatment, there were statistically significant differences in whole blood viscosity, plasma viscosity, hematocrit, erythrocyte electrodeposition rate and platelet aggregation rate between the observation and control groups of patients (P<0.05). D-dimer concentration was lower in the control group of patients at the end of 10 and 20 days of treatment compared with before with astatistically significant difference (P<0.05). D-dimer concentration was lower in the observation group of patients at the end of 5, 10 and 20 days of treatment compared with before with a statistically significant difference (P<0.05). At the end of 5, 10 and 20 days of treatment, D-dimer concentration was lower in the observation group than in the control group with a statistically significant difference (P<0.05). At the end of treatment, there was a statistically significant pre-/post-treatment difference in color Doppler-visualized lower-limb blood flow velocity in both groups of patients (P<0.05). At the end of treatment, lower-limb blood flow velocity was higher in the observation group of patients than in the control group with a statistically significant difference (P<0.05). After treatment, the total efficacy rate was markedly higher in the observation group of patients than in the control group with a statistically significant difference (P<0.05). Conclusion Acupuncture and moxibustion plus modern medication is significantly more effective than medication alone in treating lower-limb deep venous thrombosis.

Teachers are the most important resources of colleges and univesities. Setting up the teachers' competence model of medical colleges and universities is the basis of guaranting the quality of medical education, which accords with the need of the post training of teachers and can be the basis for teachers' competence assessment.

Atopic dermatitis (AD) is a chronic, relapsing, inflammatory dermatosis with a variety of clinical manifestations and difficult to cure. Currently, many AD drug candidates have entered the research and development pipeline. In order to provide technical specifications for the clinical development of AD drugs, the Center for Drug Evaluation of National Medical Products Administration released the "Technical Guidelines for Clinical Trials of Drugs for AD Treatment" (Draft for Comments) in November 2022. Non-clinical pharmacodynamics evaluation is an important research before the drug enters clinical trials. Oxazolone (OXA)- and 2,4-dinitro-fluorobenzene (DNFB)-induced models are the most popular classical hapten-induced AD murine models, but variations of modeling are existing in the methods from different studies, including sensitization sites, haptens' dosages, the period of challenges, and the skin lesions severity evaluation as well. In this study, the investigation of OXA- and DNFB-induced AD murine models with various conditions of modeling was performed to compare the characteristics of hapten-induced AD murine models in the pathological process and severity according to the appearance of AD patients, and the guidance of pharmacodynamics evaluation of AD-therapeutic drugs in clinical trials as well, which may provide a proposal for AD treatment drug candidates in the non-clinical pharmacodynamics evaluation. All animal experiments were approved by the Animal Care & Welfare Committee of Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College (approval No.: 00007782 and 00007784).

Objective： The current study was designed to investigate the polymorphisms of DNA repair gene XRCC1 in Chinese population and test the hypothesis which these genetic variations may have impact on the risk of esophageal squamous cell carcinoma(ESCC). Methods： A case control study of 222 ESCC patients and 433 control subjects (matched for age, sex) was conducted to investigate the role of two XRCC1 polymorphisms(XRCC1 26304 T and XRCC1 281152 A) in ESCC. Genotyping was performed using PCR based restriction fragment length polymorphism techniques. The adjusted odds ratio (OR) and 95％ confidence interval (CI) was calculated using multivariate logistic regression. Results： Genotype frequencies of the XRCC1 C26304T were 51.7％ (CC), 41.6％ (CT) and 6.7％ (TT) in control group. The frequency of TT allele in control group (6.7％ ) was significantly lower than that in case group (12.6％ ), with the adjusted OR for ESCC being 1.83 (95％ CI 1.03－ 3.24; P< 0.05). Furthermore, the risk of ESCC for XRCC1 26304 TT alleles appeared to be more pronounced among smokers (adjusted OR=3.1,95％ CI 1.3－ 7.2) compared to nonsmokers (adjusted OR=2.3, 95％ CI 1.0－ 5.4), and among smokers who smoked≥ 20 cigarettes/day (adjusted OR=6.4, 95％ CI 2.0－ 23.7) compared to those who smoked< 20 cigarettes/day (adjusted OR=1.0, 95％ CI 0.2－ 4.3). Genotype frequencies of the XRCC1 G28152A were 53.3％ (GG), 38.1％ (GA) and 8.6％ (AA) in control group, which were not significantly different from that in case group. Conclusion： These findings support the hypothesis which polymorphism in XRCC1 DNA repair gene contributes to the risk of developing ESCC.

To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. Methods Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony forma tion of human hematopoietic progenitor cells was identified in semi-solid culture. Results CKLF1 has obvi ous enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. Conclusion CKLF1 can pro mote the proliferation and differentiation of bone marrow cells.

BACKGROUNDEarly detection and accurate staging are crucial for planning treatment and improving survival rate of hilar cholangiocarcinomas. This study investigated the diagnostic value of a three dimensional, spoiled gradient echo, T1-weighted magnetic resonance (MR) imaging sequence (3D volumetric interpolated breath-hold examination, 3D-VIBE) in the preoperative evaluation of hilar cholangiocarcinoma.

METHODSThirty-one patients with surgically and histologically confirmed hilar cholangiocarcinomas underwent preoperative MR imaging examination. Unenhanced two-dimensional T1- and T2-weighted images, 2D MR cholangiopancreatographs (MRCP), gadolinium enhanced 3D-VIBE images in the early arterial, late arterial and portal venous phases followed by 2D T1-weighted images in the equilibrium phase were acquired. Images from 3D-VIBE, 2D T1-weighted enhanced sequences and 2D MRCP were interpreted by two abdominal radiologists through consensus reading in blind manner, focussing on the assessment of the morphological type, the longitudinal extent of tumor infiltration in the bile ducts and the involvement of neighbouring blood vessels. The accuracy of 3D-VIBE and 2D T1-weighted enhanced sequences in assessing the tumor resectability was compared.

RESULTSAll the 31 tumors were directly displayed and accurately classified on 3D-VIBE images whereas 8 periductal infiltrating tumors (8/31, 25.8%) were not depicted on 2D T1-weighted enhanced images. Using the Bismuth Corlette classification, 3D-VIBE was closer to MRCP in delineating the intraductal extent of tumor infiltration than 2D T1-weighted enhanced (28/31, 90.3%; 10/31, 32.3%; chi2 = 22.0, P < 0.05). Involvement of the hepatic artery, the portal venous trunk and their branches was shown more frequently on 3D VIBE than 2D T1-weighted enhanced images. The positive predictive value and accuracy of 3D-VIBE (84.2%; 90.3%) for assessing tumor resectability were higher than those of 2D T1-weighted enhanced images (64.0%; 71.0%, all P < 0.05).

CONCLUSIONGadolinium enhanced 3D-VIBE is better than 2D T1-weighted enhanced sequence in the preoperative assessment of the morphologicalal type, the intraductal infiltrating extent and the tumor resectability of hilar cholangiocarcinomas.

Adult ; Aged ; Bile Duct Neoplasms ; pathology ; Bile Ducts, Intrahepatic ; pathology ; Cholangiocarcinoma ; pathology ; Female ; Humans ; Imaging, Three-Dimensional ; methods ; Magnetic Resonance Imaging ; methods ; Male ; Middle Aged ; Neoplasm Staging ; Preoperative Care ; Reproducibility of Results

OBJECTIVETo explore the clinical effects and significances of a cervical anterior Hybrid technique with posterior longitudinal ligament retained in treating cervical spondylosis.

METHODSThe clinical data of 138 patients with cervical spondylosis underwent cervical anterior Hybrid surgery were retrospectively analyzed from March 2009 to March 2013. There were 52 males and 86 females,the age ranged from 36 to 58 years old with an average of 45.3 years. Course of disease was from 3 to 16 months. Cervical spondylosis classification included 22 cases with nerve root type, 68 cases with myelopathic type, 48 cases with mixed type. All patients were treated with the primary Hybrid surgery and their cervical posterior longitudinal ligaments were retained in anterior decompression. JOA score and image examination were used to evaluate clinical effect, and image examination included range of motion of the replacement segment, range of motion of the whole cervical spine, the sagittal diameter of the spinal cord before and after operation.

RESULTSAll operations were successful and operation time was 60 to 125 min (averaged 90.6 min), perioperative bleeding was 10 to 60 ml (averaged 30.1 ml). All patients were followed up from 12 to 48 months with an average of 22.2 months. All pathological segments obtained fully decompression, reserved posterior longitudinal ligament had no obvious hypertrophy, proliferation and calcification. The prosthesis had good location and the incision healed well without complications. Upper limbs root symptoms were completely relieved in the patients with cervical spondylotic radiculopathy, muscle strengths and sensations got different recovery in the patients with cervical spondylotic myelopathy. JOA score was increased from preoperative 8.62±1.22 to final follow-up 14.26±1.47 (P<0.05). Artificial intervertebral discs were stabilized in the patients, the replacement segment and range of motion of whole cervical spine were respectively (14.2± 5.6)° and (33.8±10.3)°, there was no significant differences between preoperative and postoperative (P>0.05). Spinal cord sagittal diameter was increased from preoperative (5.2±1.3) mm to postoperative (8.8±0.5) mm (P<0.05).

CONCLUSIONAnterior cervical Hybrid surgery with posterior longitudinal ligament retained can achieve fully decompression and good clinical efficacy, we recommend retaining the normal posterior longitudinal ligament in surgery as complete as possible.

Adult ; Cervical Vertebrae ; surgery ; Female ; Humans ; Longitudinal Ligaments ; surgery ; Male ; Middle Aged ; Retrospective Studies ; Spinal Fusion ; methods ; Spondylosis ; surgery