2.Cytokine storm and liver failure
Zhi CHEN ; Haihong ZHU ; Ying YANG
Journal of Clinical Hepatology 2014;30(10):981-983
Cytokine storm plays an important role in the pathogenesis of liver failure.It is closely related to hepatocyte necrosis in the clini-cal course and the prognosis of this disease.With Kupffer cells as a clue,it is elucidated that related cells and cytokines influence each other and jointly function in the development and progression of liver failure,leading to serious liver tissue damage and necrosis.Cytokine -based research can help improve early diagnosis,disease assessment,and individualized treatment for liver failure.
3.Influence of tumor suppressor gene p33~(ING1b) on growth of pancreatic carcinoma cell line SW1990
Guanzhen YU ; Ying CHEN ; Zhi ZHU ; Minghua ZHU
Academic Journal of Second Military Medical University 2000;0(10):-
Objective:To investigate the influence of p33~ING1b and wt-p53 on the biological activities of pancreatic cancer cells. Methods: Sense and anti-sense cDNAs of p33~ING1b were cloned into pcDNA3 eukaryotic expression vector separately, and the recombinant plasmids were solely or co-transfected with wt-p53 into pancreatic cell line SW1990. Flow cytometry was used to analyze the changes of cell cycle, Western blotting was used to evaluate the expression of p33~ING1b and p53 protein, and MG-P-MY staining was applied to observe cell apoptosis. Results: SW1990 cells solely transfected with sense p33~ING1b plasmid or co-transfected with wt-p53 and sense p33~ING1b plasmid expressed more p33~ING1b and had more apoptosis than the antisense p33~ING1b plasmid and mock transfected cells did (P
4.Tumor suppressor gene ING1 differentially regulates HepG2 cell growth in an isoform-dependent manner.
Zhi ZHU ; Yong-mei LI ; Zhi-gang LUO ; Ying CHEN ; Ming-hua ZHU
Chinese Journal of Pathology 2009;38(9):623-625
Alternative Splicing
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Apoptosis
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Cell Cycle
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Cell Proliferation
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Cyclin-Dependent Kinase Inhibitor p21
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metabolism
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Hep G2 Cells
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Humans
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Inhibitor of Growth Protein 1
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Intracellular Signaling Peptides and Proteins
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genetics
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metabolism
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physiology
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Nuclear Proteins
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genetics
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metabolism
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physiology
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Plasmids
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Protein Isoforms
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Recombinant Proteins
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genetics
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metabolism
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Transfection
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Tumor Suppressor Protein p53
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metabolism
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Tumor Suppressor Proteins
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genetics
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metabolism
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physiology
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bcl-2-Associated X Protein
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metabolism
5.Detection of Legionella Pneumophila in Low Respiratory Tract Infection in Children Less Than 5 Years Old
guo-hong, ZHU ; ying-shuo, WANG ; zhi-min, CHEN
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To study the present situation of legionella pneumophila(Lp) infection of low respiratory tract infection in children less than 5 years old.Methods Three hundred hospitalized patients with the diagnosis of low respiratory tract infection were enrolled in the study,191 males,109 females,aged 27 d to 5 years old,course from 1 d to 6 months.Serum antibody(IgM,IgG) of Lp serogroup 1-7 was detected by enzyme-linked immunosorbent assay(ELISA).Results In the patients,17 cases were positive,and the positive rate was 5.67%.Of the 17 children,the males(n=11) and females(n=6) did not significantly influence the positive rate(5.76% vs 5.50%).Four of them were between 27 days and 1 year old;7 were 1 to 3 years old,6 were 3 to 5 years old.The positive rates were significantly different among the 3 groups(2.53% vs 7.37% vs 12.77%) and tendentially increased following the increase of age(?2=7.8164 P
6.Association between polymorphism ofGRIN3A gene and clinical characteristic in children with Kawasaki disease
Xiangqun JIN ; Zhi XIONG ; Ying ZHU ; Lina TONG ; Liqiong WANG
Journal of Clinical Pediatrics 2015;(7):605-608
ObjectiveTo investigate the association between nucleotide polymorphisms ofGRIN3A gene and clinical characteristic of Kawasaki disease (KD) in children in Han population in central Chinese.MethodsA case-control study was performed. A total of 191 children with KD were recruited and 217 healthy children were served as controls. The distribution of SNP was determined by PCR-RFLP. Arterial lesions were detected by echocardiographic.ResultsThe distribution of three genotypes (CC, CG, GG) in SNP (rs7849782) was statistically difference between KD and control groups (P=0.034), and C allele was associated with KD susceptibility (OR=1.46, 95%CI: 1.10-1.92,P=0.007). In children with KD, the polymorphism of SNP loci was signiifcantly associated with oral mucosa lesions and coronary artery lesion (P<0.05), but not associated with conjuncti-val hyperemia, hand-foot edema, rash, and lymphadenopathy (P>0.05). The polymorphism of SNP loci was also associated with the levels of erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) (P<0.05).ConclusionThe ploymorphism of SNP loci ofGRIN3A gene (rs7849782) was associated with the susceptibility of KD. The C allele was the risk factors. The poly-morphism of SNP was associated with oral mucosa lesions and coronary artery lesion, and may affect the levels of ESR and CRP.
9.Effects of Salinity and pH on the Growth and Active Products-secreting of Aspergillus sp. F3 from the Mangrove Rhizosphere
Zhi-Wei WANG ; Ying-Ying DOU ; Xing-Wei ZHU ; Bo-Ping YE ;
Microbiology 2008;0(12):-
An Aspergillus sp. strain F3 was isolated and identified from the rhizosphere soil of mangrove plant, Rhizophora stylosa Griff in Dongzhai harbor mangrove forest conservation in China. In this study, the effects of media salinity and pH on the mycelial biomass and the ability of producing antibacterial metabo- lites from this isolate were carefully analyzed. Results showed that this isolate can grow well on the SDA medium with higher salinity (3%~9%) and higher pH (8~10). Under the modified culturing conditions, this isolate can secret the antibacterial and antitumor metabolites. The extracts of acetic ether were about 448 mg/L of the fermentation broth. The antibacterial activities of the acetic ether extract were analyzed with bacteria and fungus. Results showed this extract can suppress the growth of Staphylococcus aureus、S. epi-dermidis、Sarcina lutea、Bacillus subtilis and Escherichia coli with MIC of 31.3 ?g/mL, 31.3 ?g/mL, 7.8 ?g/mL, 7.8 ?g/mL and 125.0 ?g/mL, respectively. It can also suppress the growth of Candida albicans with MIC of 125.0 ?g/mL. Further studies uncovered the cytotoxicity of this extract against the tumor cells, such as ECV304, Lovo and HepG2 with IC50 of 3.45 ?g/mL, 4.88 ?g/mL and 14.31 ?g/mL respectively.
10.Chemical constituents from a Tibetan medicine Meconopsis horridula.
Zhi-Qin GUO ; Qiang GUO ; Zhi-Xiang ZHU ; Shui-Ying ZHANG ; Chun LI ; Xing-Yun CHAI ; Peng-Fei TU
China Journal of Chinese Materia Medica 2014;39(7):1152-1156
A phytochemical investigation on the aerial parts of a Tibetan medicine Meconopsis horridula, by solvent extraction, repeated chromatographies on silica gel, Sephadex LH-20, and preparative TLC techniques, led to the isolation of 9 compounds. By spectroscopic analysis and comparison of its 1H and 13C-NMR data with those in literatures, their structures were identified as oleracein E(1), N-( trans-p-coumaroyl) tyramine (2), chrysoeriol (3), apigenin (4), hydnocarpin (5), p-coumaric acid glucosyl ester (6), stigmast-5-ene-3beta-ylformate (7), 3beta-hydroxy-7alpha-ethoxy-24beta-ethylcholest-5-ene (8), and beta-sitosterol (9), respectively, among which compounds 6-8 were isolated from the genus for the first time,and 1,3 were isolated from the species for the first time. A MTT method was applied to evaluate the cytotoxic activity of compounds 14 against the human hepatocellular liver carcinoma cell line (HepG2), and compound 1 showed significant cytotoxicity against HepG2,with its inhibitory rate of 52.2% at 10 micromol x L(-1).
Medicine, Tibetan Traditional
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Molecular Structure
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Papaveraceae
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chemistry
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Plant Extracts
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chemistry
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Spectrometry, Mass, Electrospray Ionization