OBJECTIVETo observe clinical therapeutic effect of fuzhong (supporting the middle-jiao) manipulation of tuina for infantile anorexia.

METHODSEighty-one children with anorexia were treated with fuzhong manipulation, including abdominal kneading, acupressure at Zhongwan (CV 12), Lanmen (Extra), Tianshu (ST 25), Zusanli (ST 36), Sanyinjiao (SP 6), spine pinching, warm moxibustion and warm scraping with supporting-yang cupping, three times a week, six times as a session. Onset time, therapeutic effect and TCM symptom scores before and after treatment were observed after two sessions and differences of therapeutic effect among different patterns were compared.

RESULTSSeventy-two children had the effect in one session while 41 children had the effect within half of a session. 38 cases were clinically cured, 15 cases were markedly effective, 23 cases were effective and 5 cases were failed. The total effective rate was 93.8%. Compared with before treatment, TCM symptom scores, including appetite, food intake, complexion and defecation, were all obviously improved after treatment (all P < 0.001). The clinical cured rate was 45.2% (19/42) in the pattern of dysfunction of spleen in transportation, 45.8% (11/24) in the pattern of qi deficiency of spleen and stomach, 50.0% (4/8) in the pattern of yin deficiency of spleen and stomach, 57.1% (4/7) in the pattern of liver sufficiency and spleen insufficiency, which had no obvious differences among different patterns (all P > 0.05).

CONCLUSIONThe fuzhong manipulation of tuina has quick onset time and satisfied clinical therapeutic effect, which could obviously improve symptoms and is fit for different patterns of infantile anorexia.

Adolescent ; Anorexia ; physiopathology ; therapy ; Child ; Child, Preschool ; Eating ; Female ; Humans ; Infant ; Male ; Massage ; Meridians ; Spleen ; physiopathology ; Stomach ; physiopathology ; Yin Deficiency ; physiopathology ; therapy

Objective To compare the outcomes of selective feticide by umbilical cord ligation (UCL),bipolar cord coagulation (BCC) and radiofrequency ablation (RFA) in the treatment of complicated monochorionic twins.Methods We retrospectively analyzed all cases of complicated monochorionic twin pregnancies treated at Peking University Third Hospital from August 2008 to December 2014.The indications for surgery included severe twin-to-twin transfusion syndrome (TTTS),selective intrauterine growth restriction (sIUGR) (type Ⅱ and Ⅲ),twin reversed arterial perfusion sequence (TRAP) or discordant anomaly.One-way ANOVA,LSD t test,Mann-Whitney U test,Chi-square or Fisher's exact test were used for statistical analysis.Results (1) A total of 68 patients chose selective feticide by different techniques,including fetoscopic UCL (n=18,UCL group) and ultrasound-guided RFA (n=46,RFA group).The other four patients treated by bipolar cord coagulation (BCC) were excluded.The maternal age,proportion of assisted reproductive technology,indications,gestational age and mean birth weight all showed no differences between the two groups (P＞0.05).One case of anterior placenta was found in UCL group,fewer than in the RFA group (27 cases,36.9％)(x2=4.853).No fetal loss occurred within two weeks in UCL group,but there were seven cases (seven cases,15.2％) of earlier fetal loss in RFA group (x2=4.952).The median operation time was (63.2±22.5) min in UCL group,and longer than in the RFA group (33.3 ± 11.4) min (t=5.165),all P＜0.05.(2) The gestational age of TTTS and TRAP patients for feticide was older than patients with sIUGR and discordant anomaly [(22.7± 3.0),(22.8±3.2),(20.3 ± 2.5) and (20.4± 3.6) weeks,respectively,F=2.957,P=0.040].Fetal loss rate within two weeks in patients with discordant anomaly was higher than in other groups (4/11 vs 1/10,0/23 and 1/15,P＜0.05).The survival rate,gestational age at delivery and mean birth weight showed no significant differences among the four groups.(3)Compared with feticided fetuses at the upper uterine cavity,the fetal loss rate was higher,and the operation time,gestational age at delivery,birth weight and neonatal survival rate were lower than those performed at the lower uterine cavity,but the difference was not significant.Conclusions RFA provides similar outcomes of selective feticide in complicated monochorionic twins compared with UCL,while RFA is easier to operate.

Objective To evaluate the placental characteristics in monochorionic (MC) twin pregnancy with selective fetal growth restriction (sFGR). Methods Fifty-five placentas from women with MC twin pregnancy were included, who had terminated pregnancy in the Peking University Third Hospital between June 1, 2013 and June 1, 2014, including 23 cases with sFGR and 32 uncomplicated cases as control group. We perfused the placentas within 24 h after delivery, and pigment of four different colors was used to perfuse the umbilical arteries and veins of both twins and determine the types of vascular anastomosis. Umbilical cord insertion, placental territory discordance (PTD, the territory difference between two placentas/the bigger one), and the type, number and diameter of placental superficial vascular anastomosis were analyzed using two independent samples t-test, nonparametric test,χ2 test or Fisher's exact test. Results The PTD was 0.60(0.10-0.80) vs 0.22(0.00-0.90) in sFGR group and control group (Z=-3.913) respectively, and the proportion of placenta with uneven share was 91.3%(21/23) vs 50.0%(16/32) (Fisher's exact test), which were significantly higher in sFGR group (both P < 0.01). The proportion of non-central cord insertion was 82.6% (19/23), 13.0% (3/23) and 40.6% (26/64), respectively, in smaller fetus of sFGR, bigger fetus of sFGR and control group, which was significantly higher in smaller fetus of sFGR than in the other two groups (Fisher's exact test, both P < 0.01). The proportion of arterioarterial (AA), arteriovenous (AV) and venovenous (VV) anastomosis in sFGR group and control group was 78.3%(18/23) vs 75.0%(24/32), 82.6%(19/23) vs 71.9%(23/32), and 17.4%(4/23) vs 15.6%(5/32);there were no significant differences between two groups (Fisher's exact test,all P>0.05). The number of AA, AV and VV anastomosis in sFGR group and control group was 1.0 (0.0-2.0) vs 1.0 (0.0-4.0), 3.0 (0.0-10.0) vs 2.0 (0.0-5.0), and 0.0 (0.0-1.0) vs 0.0 (0.0-3.0) (Z=-0.256, -0.142 and -0.123);the total diameter of AA, AV and VV anastomosis was 2.7 (0.0-7.0) vs 2.2 (0.0-9.7), 4.0 (0.0-13.7) vs 3.4 (0.0-11.5), and 0.0 (0.0-7.9) vs 0.0 (0.0-7.1) mm (Z=-0.070, -0.087 and -0.087);there were no significant differences between two groups (all P>0.05). The total number of all anastomosis was 3.5 (0.0-10.0) vs 3.5 (0.0-6.0) (Z= - 0.567); the total diameter of all anastomosis was 6.9 (0.0-22.4) vs 5.9 (0.0-17.1) mm (Z= - 0.556); there were no significant differences between two groups (all P>0.05). Conclusions Placental sharing discordance and non-central cord insertion may be the risk factors for MC pregnancies complicated with sFGR.

Objective To study the characteristics of life events, coping styles and social supports in anxiety disorders patients. Methods 50 patients with generalized anxiety disorders (GAD), 44 patients with obsessivecompulsive disorders (OCD),23 patients with social anxiety disorders (SAD) and 99 healthy people were assessed by life event scale(LES),simplified coping style questionnaire(SCSQ) and social support rating scale (SSRS). Results Compared with healthy people, patients with GAD, OCD and SAD were all had higher scores of total life events (GAD 22.46±18.44;OCD 23.68±28.31;SAD 17.22±14.97;Healthy People 6.64±9.71)( F =14.216,P =0.000) and negative life events(GAD 18.76±17.99;OCD 20.02±24.98;SAD 12.00±13.16;Healthy People 4.56±7.51)( F =14.391,P =0.000).All patients were apt to choose positive styles less( F =16.505,P =0.000) and got less social supports totally ( F =26.506,P =0.000). The objective social supports( F =7.494,P =0.000)and subjective social supports ( F =26.985,P =0.000) were less and the utilization of social supports was lower ( F =13.931,P =0.000) in patients.Conclusions 1. Anxiety disorders patients often experience more or more serious life events, especially negative life events, are more likely to choose negative coping styles, and get less social supports. 2. Anxiety disorders may be related with people's characteristics of life events, coping styles and social supports.

Objective To study the symptom dimensions of Chinese patients with obsessive-compulsive disorder(OCD). Methods Symptom checklist in expert versions of Dimensional Yale-Brown Obsessive-Compul-sive Scale (DY-BOCS) was used to assess the symptom dimensions of 139 OCD patients. Results Nearly half of the patients had one dimension (50.4%). The frequency of patients with two or three dimensions was 24.5% and 14.4%. Patients with four to seven dimensions were very few and the frequency were 5.8% ,3.6% ,0.7% and 0.7% ,respectively. The most common symptom dimensions were symmetry (67.6%), contamination (43.2%) and aggressive (31.7%). The frequency of patients with miscellaneous, somatic, sex and religious, hoarding di-mension were 18.7%, 11.5% , 10.8% ,8.6% ,respectively. The frequency of male patients with symmetry dimen-sion was higher than that of the female patients(75.8%/52.1%, P<0.01), and the frequency of female patients with contamination dimension was higher than that of male patients (56.3%/36.3%, P<0.05). There were no differences in other dimensions between male and female patients. The early-onset patients had a greater of symp-toms in the symmetry dimension than late-onset patients and there were no differences between these two groups in other dimensions (77.6% vs 60.5%, P<0.05 ) . Conclusion Preliminary data suggest that the symptom di-mension approach may aid in the identification of subtype of OCD.

Objective To understand the clinical characteristics of systemic lupus erythematosus with Antiphospholipid syndrome (SLE-APS).Methods The clinical data of 39 cases of SLE-APS were collected and analyzed retrospectively. Results Thirty-one patients had 48 thrombosis episodes in total, among which the most common presenting manifestations were deep venous thrombosis and stroke. 12 of 26 married women had fetal morbidity. The prevalence of anticardio lipin antibody (aCL) and lupus anticoagulant ( LA) was 72% and 41% , respectively. 24 patients had SLE at first,on an average of 9. 5 years later they had the characteristics of APS, 12 patients had thrombosis episodes or fetal morbidities ,4. 8 years later they developed into SLE,and 3 patients had the characteristics of SLE and APS from the onset The SLEDAI was less than 5 in five cases when they had thrombosis episodes or fetal morbidities.Conclusions The incidence of thrombosis episodes or fetal morbidities increased in SLE-APS patients. APS occurred before, after or at the same time with SLE. SLE patients might have the clinical features of APS during the steady stage. It's very important to ask the medical history in detail and measure aCL in lupus patients to find the risk factor of APS and prevent the APS' occurrence.

The optic-fiber sensor technology combined with near-infrared diffuse reflection spectroscopy was applied to directly analyze Peganum harmala and identify different origin of P. harmala on the basis of principal component analysis, clustering analysis, SIMCA method, which resulted in the establishment of a new method to rapidly and nondestructively identify the origin of P. harmala. The original full wavelength spectrum for principal component analysis and the score of first two principal components can distinguish four origins of P. harmala basically. In the wavelength range of 866-2,507 nm, MSC as pretreatment method to establish the best model of clustering analysis to forecast the samples with the accuracy of 91.67%, can distinguish the four origins of P. harmala while in the wavelength of 1,085-2,507 nm, normalization method as pretreatment methods to establish a best model of SIMCA to forecast the sample, all the samples except for the changji sample have been identified with a total recognition rate of 97.22%. The results show that using near infrared diffuse reflectance spectroscopy combined with SIMCA is the best method that can be effectively used to identify the P. harmala.
Cluster Analysis ; Models, Theoretical ; Peganum ; chemistry ; Principal Component Analysis ; Spectroscopy, Near-Infrared ; methods

Animals ; DNA Methylation ; Epigenesis, Genetic ; Histones ; metabolism ; Humans ; Neoplasms ; genetics ; pathology ; Neoplastic Stem Cells ; metabolism ; pathology ; RNA, Untranslated

BACKGROUND: Angiogenesis in atherosclerotic plaque occurred on the basis of atherosclerotic lesions, and the new formed blood vessels promoted the development of angiogenesis. Soluble vascular endothelial growth factor receptor 1 (sFlt-1) gene transfection reduces neointimal formation after vascular injury in rabbits, also reduces early vascular inflammation and proliferation, and the formation of neointima lately.
OBJECTIVE: To observe the effects of highly expressed sFlt-1 on atherosclerotic plaque.
METHODS: A total of 48 male New Zealand rabbits were divided into four groups randomly: normal control group (n=8), sham operation group (n=8), bal oon injury+high lipid diet+pEGFP-N1 group (n=16) and bal oon injury+high lipid diet+pEGFP-N1-sFlt-1 group (n=16). Normal control group and sham operation group were supplied with normal diet. Bal oon injury+high lipid diet+pEGFP-N1 group and bal oon injury+high lipid diet+pEGFP-N1-sFlt-1 group were supplied with high lipid diet and injured by bal oon 2 weeks later. Bal oon injury+high lipid diet+pEGFP-N1 group was transfected with pEGFP-N1 and bal oon injury+high lipid diet+pEGFP-N1-sFlt-1 group was transfected with pEGFP-N1-sFlt-1.
RESULTS AND CONCLUSION: In the bal oon injury+high lipid diet+pEGFP-N1 group and bal oon injury+high lipid diet+pEGFP-N1-sFlt-1 group, blood lipid levels apparently increased. At 3 days after model establishment, sFlt-1 expression levels noticeably increased, and atherosclerotic plaque formed to different degrees. Plaque area, plaque perimeter, plaque maximum thickness and the number of positive-cel s within the plaque were significantly less in the bal oon injury+high lipid diet+pEGFP-N1-sFlt-1 group than in the bal oon injury+high lipid diet+pEGFP-N1 group. These results confirm that sFlt-1 gene may express effectively in iliac artery wal of rabbit, inhibited the formation of atherosclerotic plaque and delayed the development of atherosclerotic plaque.

Objective To evaluate the clinical efficacy and safety of chemotherapy weekly in the chemoradiotherapy of cervical cancer.Methods Between Jan 2003 and Oct 2007,168 patients with stage Ⅲ B squamous cell carcinoma of the uterine cervix in Chongqing Cancer Hospital were divided into two groups randomly.The observation group,total 86 patients receiving radiotherapy concomitant with weekly cisplatin and taxol;the matched group,total 82 patients,receiving radiotherapy concomitant with cisplatin plus taxol every 3 weeks.There were no statistical difference in the clinical and pathological characteristics between the two groups. We compare the 5 years survival rate and side reaction of the two groups.Results The 5 years survival rate of the observation group and the matched group were respectively 52.3%(45/86)and 42.7%(35/82),in which there was no significant difference (P >0.05).The response rate(CR+PR)were respectively 94.2%(81/86)and 92.7%(76/82)in the observation group and the matched group,in which there was no significant difference (P >0.05).Grade Ⅲ acute gastrointestinal toxicities (nausea and vomi-ting)in the matched group were exactly higher than that in the observation group [19.5%(16/82)vs.8.1% (7/86),P <0.05]. Moreover,Grade Ⅲ myelosuppression in the matched group were exactly higher than that in the observation group [20.7%(17/82) vs.9.3% (8/86),P <0.05].there were no significant difference of the incidence of the radiocystitis,radiation proctitis and radio-dermatitis between the two groups.But the Radiotherapy completion time in the matched group were exactly longer than that in the observation group [(61.12±6.71)days vs.(54.72±4.76)days,P <0.05].Conclusion The toxicity of the observation group was lower than the matched group.Moreover the efficacy of observation group is similar to the matched group.