ATP-tumor chemosensitivity assay(ATP-TCA)is a tumor chemosensitivity assay in vitro.Beacause of the special merites of A11P-TCA,it is seemed to be the most promising and effective method of tumor chemosensitivity in vitro.Application of ATP-TCA in clinic has got significant effects.ATP-TCA is a good method for individualized chemotherapy.

Under the environment of network,the service mode of subject librarian becomes an important strategy of intelligence serves in university libraries.To provide reference for other librarians,this paper summarizes services provided for the academic researchers by using Web of Knowledge platform resources.

Objective To study the effect of binocular vision function training for fusion in the eye position and three levels of eye function in adult patients after concomitant strabismus surgery. Methods 56 cases with postoperative concomitant strabismus were randomly divided into training group and control group . In the training group, patients were trained with software to enhance the fusion. After training for 7, 30, 180 and 365 days, the eye position, the fusion and the stereoesthesia were observed and analyzed. Results After training for 365 days, the deteriorative ratio in the training group is 3%, which is significantly lower than control group (23%)(P<0.05). The correction ratio in eye position is 20%, which is significantly higher than control group (4%)(P<0.05). The recovery ratio in binocular vision function is 53%, which is significantly higher than control group (15%)(P<0.05). The recovery ratio in stereaeuity is 46%, which is significantly higher than control group (11%)(P<0.05). Conclusions Fusion training can enhance the recovery of fusion and stereaeuity, decrease the deteriorative ratio, and correct the eye position after surgery in concomitant strabismus patients.

Objective To investigate the relationship between renal function and lipid metabolism in patients with chronic renal disease .Methods A total of 76 patients with chronic renal disease were enrolled and classified into chronic renal failure(CRF)group (29 cases) ,chronic nephritis(CN)group(21 cases) ,diabetic nephropathy (DN) group(14 cases) and nephrotic syndrome (NS) group (12 cases) ,and 65 healthy subjects were enrolled in control group ,the relationships between renal function ,lipid metabolism , hypersensitive C-reactive protein (hs-CRP) and 24 hours urinary albumin excretion rate (UAER) were analyzed .Results Urea ni-trogen(Urea) ,serum Cystatin C(CysC) ,cretinine(Cr) ,uric acid(UA) ,β2-microglobulin(β2-MG) ,hs-CRP ,low density lipoprotein (LDL-c) .Lp(a) .24h UAER were significantly higher in CN group;Urea ,Cr ,β2-MG ,CysC ,hs-CRP ,ApoA ,lipoprotein A[Lp(a)] , 24 h UAER were significant higher in DN group;Urea ,hs-CRP ,TC ,TG ,HDL-c ,LDL-c ,ApoB ,Lp(a) ,24h UAER were significant-ly highly in NS group;Urea ,Cr ,UA ,β2-MG ,CysC ,hs-CRP ,TG ,LDL-c ,Apolipoprotein b(ApoB) ,Lp(a) ,24 h UAER were signifi-cantly higher in CRF group .Conclusion With the changes of renal function in patients with chronic renal diease ,the lipid metabo-lism and hs-CRP also change .

Objective:To investigate the difference of biological characteristics and responses to PDGF-BB between human ovarian carcinoma-derived microvascular endothelial cells and human umbilical vein endothelial cells.Methods:The ovarian carcinoma-derived microvascular endothelial cells were isolated and purifed from ovarian carcinoma tissues.Compared to the human umbilical vein endothelial cells,the biological characteristics and responses to PDGF-BB were observed in OdMEC for the changes of growth,PCNA and VEGF expression.Results:20ng/ml PDGF-BB could enhance both kinds of endothelial cells to proliferate,express PCNA and VEGF.The proliferation and PCNA protein expression in HUVEC were augmented more significantly than those in OdMEC,the protein and mRNA of VEGF expression were augmented more significantly than those in HUVEC.Conclusion:The responses of OdMEC to PDGF-BB were significantly different from those of HUVEC,which provides experiment basis for research on ovarian carcinoma vascularization and antiangiogenesis therapy.

Objective To investigate the effect of selectiveα1 adrenergic receptor blocking agent in the treatment of bladder neck contracture caused by refractory urinary tract infection in elder.Methods 70 cases of bladder neck contracture were selected and randomly divided into two groups, 35 cases each.Two groups received Ceftriaxone sodium 1 g qd at acute episode,Levofloxacin Hydrochloride 0.5 g tid at non acute phase,control group received the treatment of urethral dilatation, Terazosin hydrochloride 10 mg qd, the experiment group received more with Tamsulosin Hydrochloride Sustained 0.2 mg qd.Two groups were both treated for four weeks.Urinary dynamics index, international prostate symptom score,quality of life score, clinical symptom disappearance time, the number of daily spasm and adverse reactions were compared.Results After treatment, the total effective rate in the experiment group(94.29%) was higher than the control group(65.71%)(P<0.05).Maximum and the average urinary flow rates in two groups increased after treatment(P<0.05), residual urine volume, maximum urethral closure pressure decreased(P<0.05), international prostate symptoms and quality of life score decreased(P<0.05); and compared with the control group, maximum and the average urinary flow rates in the experiment group were higher(P<0.05), residual urine volume, maximum urethral closure pressure were lower(P<0.05), international prostate symptoms and quality of life score were lower(P<0.05), and the average duration of symptoms of bladder neck spasm and the number of daily spasm in the experiment group were significantly shorter than the control group ( P <0.05 ).There was no significant difference in adverse reactions between the two groups. Conclusion Selectiveα1 adrenergic receptor blocking agent in the treatment of bladder neck contracture caused by refractory urinary tract infection in elder was effective, and it can improve the patient's urine flow rate, reduce the residual urine level, increase the quality of life.

OBJECTIVE:To provide reference for revising the standards of primaquine phosphate in International Pharmacopoe-ia (the fifth edition). METHODS:Based on inspection standards for primaquine phosphate and its preparations in different coun-tries,HPLC,perchloric acid potentiometric titration and sodium nitrite dead-stop titration were adopted to determine and compare the contents of primaquine phosphate. RESULTS:In HPLC,the linear range of primaquine phosphate was 128.1-384.2 μg/ml(r=0.999 9);the limit of detection was 0.18 μg/ml,the limit of quantification was 0.59 μg/ml;RSDs of precision,stability and repro-ducibility tests were lower than 1%;recovery was 99.42%-101.14%(RSD=0.6,n=9);destructive test and durability test showed good specificity;the contents of 3 batches of samples were 92.1%,92.3% and 92.0%,respectively. In perchloric acid potentiomet-ric titration,RSDs of precision and reproducibility tests were 1.6%;the titration jump was not obvious;the contents were 99.1%, 99.7% and 98.7%,respectively. In sodium nitrite dead-stop titration,RSDs of precision and reproducibility tests were lower than 1%;the titration jump was obvious;the contents were 96.9%,97.1% and 96.7%,respectively. Sodium nitrite external indicator ti-tration showed better precision than dead-stop titration;the contents were 99.6%,100.0% and 99.5%,respectively. CONCLU-SIONS:Perchloric acid potertiometric titration is simple and rapid,but with relatively poor precision and reproducibility,and it contains quinocide. Sodium nitrite dead-stop titration is simple with good precision and reproducibility but long reaction time,and it also contains quinocide,the HPLC method shows good separation and high accuracy,can effectively separate the quinocide in primaquine phosphate,and it is suitable for the quality control of primaquine phosphate.

The paper introduces using MedlineR to carry out data mining from Medline, for observing the gene relation, and using Pajek to realize the visualization of the results. Practice proofs that MedlineR could help researchers come up with assumptions in the field that they are interested in, and get a visual fingerprint. These results are the bases to go to more complex literature data mining and an important supplementary to experimental methods.

Objective To investigate the influence of surface grafting density of polyethylene glycol (PEG) molecules on the interactions of nanoparticle with the biomembrane.Methods The dynamics of polymer as well as its shielding effect on the wrap of the membrane were investigated using coarse-grained (CG) molecular dynamics simulations.A coarse grained model force field,MARTINI,was employed for its ability to reproduce experimental properties of various lipid dynamics and some kinds of polymer as well.Results As a result,structural variations and energy changes of grafted PEG molecules on the nanoparticles,adsorbing dynamics of PEGs on the membrane as well as the wrap rates of the membrane were obtained to shed light on the PEGylation-mediated shielding mechanism in the adsorption of PEGylated nanoparticles on the membrane.Conclusions The surface grafting density of PEGylated nanoparicles has been found to play a crucial role in the stealth shielding behavior of PEGylated nanocarriers.These studies are consistent with experimental observations and to some extent give a molecular level interpretation of the macroscale observations of prolonged circulation half-life of stealth nanocarriers.

For a valid control of the finance management, the management system is developed again aiming at finance related control links and key control points. In this way, the control is rigorous and integral with simple procedures for check and examination. The key control point is set oriented to note management. Real-time operation is controlled. Post output processing is controlled. With business income and business expenditure as the main line, the control process of the related links are built up. Computer maintenance system is controlled.