Objective To investigate the infection statue of new drug experimenters with hepatitis B,as well as liver fuction in 6 kind of infection modes of serum HBV,and to provide evidence for clinical therapy of hepatitis B.Methods 5 immunological HBV markers were measured with ELISA in serum sample of 550 patients before new drug test. Velocity method was applied to detecting serum TBiL,ALT,AST,γ-GT and GLO.Results New drug experimenters with hepatitis B mainly suffered from "small three positive" [HBsAg (+),anti-HBe (+) and anti-HBc (+)] (52.9%),followed by "big three positive" [HBsAg (+),HBeAg (+) and anti-HBc (+)] (28.2%). The rate of hepatic function abnormality was significantly higher in "small three positive" group than that of the other groups (P<0.01),while the rate of hepatic function abnormality was much higher in "big three positive" group than that of the other groups except "big three positive" group (P<0.05).Conclusion In 550 cases of new drug experimenters,HBV are all positive,mostly in "small three positive" or "big three positive" mode. The rate of hepatic function abnormality is markedly elevated in 6 kind of infected modes,in which "big three positive" mode is the highest,followed by "1,3" mode and"small three positive" mode.

OBJECTIVE:To establish the quality standard of Kangshiming mixture.METHODS:The Phellodendron chinense,Citrus anrantium,Paeonia lactiflora,Angelica sinensis,and Pueraria lobata in Kangshiming mixture were identified by HPTLC and the contents of Naringin and Ammonium glycyrrhizinate were determined by UFLC.RESULTS:The spots in the HPTLC were clear and well-separated yet free of interference of negative sample.The linear ranges of Naringin and Ammonium glycyrrhizinate were 0.015～0.135 ?g(r=0.999 9) and 0.1～0.9 ?g(r=0.999 9),respectively,and their recovery rates were 97.78%(RSD=2.19%,n=6) and 101.99%(1.95%,n=6),respectively.CONCLUSION:The established standard is applicable for the quality control of Kangshiming mixture.

Objective To explore the clinical curative effect of Danzhen Headache Capsule on treatment of hypertension with headache.Methods 68 cases of hypertension with headache were divided into treatment group (n =38)and control group(n =30)in accordance with the random number table.The control group received the con-ventional treatment,and the treatment group received addiontional Danzhen Headache Capsule.Clinical curative effects of the two groups were observed.Results After treatment,the treatment group total effective rate was higher than that in the control group(84.21% vs 53.33%).There were statistical differences between the groups(χ2 =7.992,P <0.05),and with non-toxic side effects.Conclusion Proprietary Chinese medicine has curative effect on high blood pressure with headache,Danzhen headache capsule can improve the clinical symptoms as the preferred drug.It is worthy of further clinical research and extension.

Objective To explore the effect of early airway evaluation in critical patients in emergency room.Methods One hundred and sixty-six critically ill patients with routine intubation,according to the condition of early airway evaluation,were divided into early-estimate group (86 cases) and control group (80 cases).The patients in control group due to illness change needed to build artificial airway,tracheal intubation for the first time was done by the doctor on duty,and before intubation grading evaluation of airway was made.The patients in early-estimate group in emergency room had been early estimated using improved Mallampati classification and Thyromental distance by the doctor on duty.The number of cases with first successful endotracheal intubation,endotracheal intubation time,cases with forecast of difficult airway,the number of cases forecast of difficult airway intubation with first time successful endotracheal intubation,the number of cases failed in intubation and needed emergency tracheotomy,and the time of relatively stable vital signs,emergency department mortality were recorded in two groups.Results The endotracheal intubation time,forecast of difficult airway,rate of failing in intubation and needing emergency tracheostomy tube had no statistical significance (P ＞ 0.05).The first time success rate of tracheal intubation and forecast of difficult airway intubation in early-estimate group were higher than those in control group [69.4％ (59/85)vs.40.0％ (32/80),80.0％ (28/35) vs.32.3％ (10/31)],the time of rehtively stable vital signs was shorter than that in control group[(89.5 ± 23.2) min vs.(110.4 ± 48.2) min],emergency department mortality was lower than that in control group [8.2％(7/85) vs.18.8％(15/80)],and the difference was statistically significant (P ＜ 0.05).Conclusion Early-estimation in the emergency room for the critical patients and preparing for the corresponding work is helpful to improve the success rate of first intubation in the patients with difficult airway,and to restore stability to achieve the safe transfer standard as soon as possible.

Objective To evaluate early hemopeffusion in the patients of acute organophosphate poisoning from economic.Methods Forty patients with acute organophosphate poisoning were divided into early hemoperfusion group (20 cases) and control group (20 cases) according to the condition of early hemoperfusion in three hours after the diagnosis.The date of basal clinical conditions,treatment,prognosis and cost in the 2 groups were observed.Results There were no statistical differences in sex,age,the level of initial acetylcholine esterase (ChE) and degree of poisoning between the 2 groups (P ＞ 0.05).The patients of the 2 groups underwent at least 1 time hemoperfusion.The time of beginning hemoperfusion in early hemoperfusion group and control group were (1.6 ±0.9) h and (6.4 ±3.6) h,and there was statistical difference (P ＜0.05).The time of ChE recovery and length of stay in early hemoperfusion group were significantly lower than those in control group [(8.5 ± 2.1) d vs.(10.0 ± 1.8) d,(10.5 ± 4.2) d vs.(12.3 ± 5.4) d],and there were statistical differences (P ＜0.05).In early hemoperfusion group 5 cases needed rehemoperfusion,and 4 cases needed mechanical ventilation,in control group were 8 and 5 cases respectively.There was no death in the duration of hospital stay and during 28 d in early hemoperfusion group,but in control group death in the duration of hospital stay was 1 case and death during 28 d was 1 case.The total hospitalization cost,daily treatment cost,health care cost and drug cost in early hemoperfusion group were significantly lower than those in control group [(17 231.75 ± 5 411.45) yuan vs.(21 394.52 ± 6 049.81)yuan,(1 412.25 ±169.33) yuan vs.(1 579.41 ±307.19) yuan,(4 309.22 ±1 490.12)yuan vs.(5 581.71 ± 1 681.00) yuan,(8 223.41 ± 1 426.10) yuan vs.(9 790.02 ± 1 909.24) yuan],and there were statistical differences (P ＜ 0.05).Conclusion The patients of acute organophosphate poisoning could be benefit from early hemoperfusion,because early hemoperfusion can shorten the recovery time of ChE,the length of stay,and reduce the hospitalization cost.

Objective To increase the anti-tumor effect of 5-ALA-mediated photodynamic therapy, PDT was performed at 635nm followed by an additional irradiation at 670nm, and the effectiveness was studied. Methods PDT was performed at single irradiation (635nm or 670nm), and symphysial irradiation (635nm+670nm), and its effectiveness was studied using PpIXsolutions, tumor cells (9L and HeLa), andHeLa tumor-bearing mice. Results When a Pp IX solution was irradiated at 635 nm or 670nm, singlet oxygen counts were almost similar. Symphysial irradiation -mediated singlet oxygen generation was higher in 9L cells than HeLa cells and it did not produce an additional PDT effect in HeLa tumors. Conclusion It was suggested as the low uptake of 5-ALA in HeLa cells, and the symphysial irradiation can't increase the anti-tumor effects in 5-ALA-mediated PDT. Further study is necessary to obtain the irradiation method for the efficient application of PDT.

Objective To explore the relationship between high-sensitivity C-reactive protein (hs-CRP) and the occurrence of certain diseases such as coronary heart disease (CHD) and acute myocardial infarction (AMI).Methods Sixty-one CHD patients, 45 AMI patients (patients group, excluding high hs-CRP level diseases caused by other etiological factors) and 35 healthy controls (control group) were enrolled in the study. The serum level of hs-CRP and myocardial zymogram was measured in the Hitachi 7060 automatic analyzer by latex-enhance immunoturbidimetry for all subjects, and cTnI of AMI group was detected with immunofluorescence method at the same time. The difference of hs-CRP level was compared and analyzed between control group and patients group; control group before and after treatment. Moreover, the relationship between hs-CRP and myocardial zymogram as well as cTnI was analyzed.Results The level of hs-CRP was significantly higher in patients group than that in control group (P<0.01). There was statistical difference of hs-CRP level before and after treatment in control group (P<0.01). hs-CRP was positively correlative with myocardial zymogram and cTnI.Conclusion The serum level of hs-CRP is significantly elevated in CHD and AMI patients, indicating the measurement of hs-CRP contributes to auxiliary diagnosis, therapeutic effect observation, and prognosis evaluation.

Objective To learn the gene mutation type and prevalence of thalassemia in the Taishan area and to provide basis for the prevention and treatment of local thalassemia .Methods 1 383 patients who visited the hospital for thalassemia genetic test from January to December 2014 were enrolled for this study .α‐andβ‐genes were determined by using PCR and membrane hybridization methods .Results Of the 1 383 patients ,595 were diagnosed with thalassemia (positive rate was 43 .02% ) ,including 386 cases (27 .91% ) of α‐thalassemia ,183 cases(13 .23% ) of β‐thalassemia and 26 cases(1 .88% ) of αβ‐thalassemia .- -SEA/αα(accounted for 64 .25% ) was the major genotype of α‐thalassemia ,the major genotype of β‐thalassemia wasβ41 -42/β(accounted for 28 .42% ) , and - -SEA/αα,β41 -42/βwas the major genotype of αβ‐thalassemia (accounted for 19 .23% ) .Conclusion Taishan is located in a high risk area of thalassemia ,it is of great significance to perform genetic diagnosis of thalassemia among reproductive population so as to reduce the birth rate of children with thalassemia in the area .

Micro-invasive thoracic surgery has become new trends of thoracic surgery,and video-assisted thoracic surgery (VATS),which has the advantage of less trauma,rapid recovery and fewer postoperative complications,has become the mainstream of lung cancer resection.With the development of technology and surgical instruments,VATS has taken the place of open procedure,and the postoperatively follow-up also showed that both the two procedures have similar results in long-term survival rate and so on.With years of rapid develop,now the traditional VATS has nearly been perfect,the thoracic doctors eagerly try to find a new kind of micro-invasive surgery.It is in this background that Uniportal video-assisted thoracic surgery is born and it has been received by more and more operators.Uniportal VATS is not only inferior than multiportal VATS in Postoperative survival,but also has the advantage in reliving post thoracotomy chest pain as it only has one incision.In this paper,the author would like to summarize up the newest development of uniportal VATS in recently years.

Objective:To study on effect of Gutai Decoction on the abortion rate of in vitro fertilization and embryo transplantation(IVF-ET).Methods:237 cases with ?-human chorionic gonadotropin(B-HCG) ≥25IU/L 14 days after the transplantation were divided into Treatment group A (TCM administration after the transplantation),Treatment group B(TCM administration after pregnancy) and western medicine control group.Their abortion rates were observed.Results:The abortion rate was 13.08%,15% and 24% in treatment group A,B and the control group respectively,with significant difference between the treatment groups and the control group(P