Objective To construct a safe data-back-up system with disaster-tolerant ability. Methods Based on the concept of data back -up, the paper puts forward several data back -up modes in common use, and carries out corresponding comparison. Results The author synthesized these back -up modes into an ideal, feasible, and highly secure system architecture with disaster-tolerant ability. Conclusion Constructing a safe data-back-up system are becoming the mostly indispensable elements for the hospital informationization. It can avoid the single fault in the hardware level, and also can repair the logical data fault in the software level.

Objective To improve the reliability of the core database system by ameliorating the scheme of the server system architecture. Methods Combined with practice of the hospital informatization, the security and potential hole of the database server system were analyzed and evaluated. Results Aimed to deal with the security hidden-trouble, a mini-server was adopted instead of PC server, while SAN(Storage Area Network) was utilized instead of RAID-5, and mirror copy technique was used to implement the real-time hot copy of the data. Conclusion Consequently the reliability of the system has been greatly improved.

Objective To explore the theory and measures of armymen's clinical flow through information technology based on an exclusive armymen clinical area.Methods On the basis of No.1 Military Medical Project,such system was developed as automatic medical service classification & guide system,clinic backstage & auto-medicine provision system and comprehensive clinical service system in order to optimize medical service for armymen.Results By integrating the armymen's clinical information system,Navy General Hospital optimized comprehensively the clinical flow of registration,waiting,diagnosis,pricing,medicine pick-up,etc.Therefore,the efficiency of medical service for the army was greatly improved.Conlusion The full application of information technology will make an improvement in clinical flow and provide better service to army patients.

Animals ; Disease Models, Animal ; Female ; Fibrosis ; metabolism ; pathology ; Hydroxyproline ; metabolism ; Lung ; drug effects ; metabolism ; pathology ; Mice ; Thrombospondin 1 ; pharmacology

Endoscopy ; Humans ; Male ; Middle Aged ; Myoepithelioma ; surgery ; Nasal Septum ; pathology ; Nose Neoplasms ; surgery

The mRNA and protein expression of thymidylate synthase (TS), thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) and their relationship with prognosis were investigated. Real-time quantitative RT-PCR (Taqman) was used to detect the mRNA expression of TS, TP and DPD in formalin-fixed and paraffin-embedded 106 samples of epithelial ovarian cancer and 29 normal ovaries. A TATA box-binding protein (TBP) was used as an endogenous reference gene. A relationship between TS, TP, DPD expression and clinicopathologic features was investigated. The protein location and expression of TS, TP and DPD was examined in the same patients by an avidin-biotin-peroxidase immunohistochemistry. TS and TP mRNA expression levels were significantly higher in tumor group than in normal controls, with the average value of TS and TP mRNA being 6.14+/-0.62 and 0.59+/-0.06 in tumor tissue, and 0.71+/-0.14 and 0.16+/-0.04 in normal tissue, respectively. DPD mRNA expression levels were significantly lower in tumor group (0.11+/-0.02) than in normal controls (0.38+/-0.05). There was statistically significant difference in TS and TP mRNA expression levels among different pathological grades and clinical stages (P<0.05), but histological subtype was not significantly associated with TS and TP mRNA expression. DPD gene expression was not significantly associated with any clinicopathological parameters. Immunohistochemistry revealed that TP protein was mainly distributed in nucleus, and TS and DPD mainly in cytoplasm. The protein expression intensity of TS, TP and DPD was coincided with the mRNA expression levels. It was concluded that TS, TP mRNA and protein expression levels were significantly higher in epithelial ovarian cancer, and DPD mRNA and protein expression levels were significantly lower. The expression levels of TS and DPD were related to the patients' prognosis and survival. Combined gene expression levels of TS, TP and DPD represent a new variable to predict the clinical outcome in ovarian cancer. The association of TS, TP and DPD expression levels with survival suggests an importance of these genes for tumor occurrence and progression.

Objective To discuss how to improve the stability,reliability and security of the core servers of hospital information system.Methods Flaws on security were analyzed objectively in hospital server system adopting 'PC server & local hardisk' scheme.New schemes were put forward including both hot standby scheme based on concentrated storage and full redundancy scheme based on'minicomputer & diskette array'.Results It is proved that the full redundancy scheme is reasonable in theory,and usable in practice.Conclusion The mode of'minicomputer & diskette array' can effectively enhance the stability,reliability and safety of core servers.

Objective To develop a software through which transfusion labels can be created and printed automatically based on No.1 Military Medical Project,in order to resolve the problems of time-and-labor-consuming and errors due to transcription. Methods Based on No.1 Military Medical Project,the software of creating and printing transfusion labels was developed using PB computer programming language. Results The software can effectively avoid the errors due to copying transfusion labels by hand and save much time and effort. It optimizes nurses' working procedure. Conclusion The software is very practical for all the hospitals using the No.1 Military Medical Project.

Objective To evaluate the effect of dexmedetomidine on myocardial injury in pediatric patients undergoing living-related liver transplantation. Methods Fifty-eight pediatric patients of both sexes,aged 5-20 months,weighing 4.5-15.0kg,of American Society of Anesthesiologists physical status Ⅲ or Ⅳ,scheduled for elective living-related liver transplantation,were divided into 2 groups(n=29 each)using a random number table:control group(group C)and dexmedetomidine group(group D).In group D,dexmedetomidine was intravenously infused at a dose of 0.5 μg/kg over 10min starting from the time point immediately before skin incision,followed by an infusion of 0.8 μg·kg-1·h-1 until the end of surgery. The equal volume of normal saline was given instead in group C. Immediately before skin incision(baseline,T0),at 10min of anhepatic phase(T1),at 30min of neohepatic phase(T2)and at the end of surgery(T3),blood samples were obtained from the central vein for determination of serum cardiac troponin I(cTnI),lactate dehydrogenase(LDH),alpha-hydroxybutyrate dehydrogenase(α-HBDH),interleukin-6(IL-6)and IL-10 concentrations. The changing rate of serum cTnI concentrations were calculated at T2. The occurrence of myocardial ischemia and ventricular premature beat and requirement for dopamine were recorded during surgery. Results Compared with the baseline at T0,the serum concentrations of cTnI,LDH and α-HBDH were significantly increased at T2,3,and the serum concentrations of IL-6 and IL-10 were increased at T1-3 in both groups(P<0.05).Compared with group C,the serum concentrations of cTnI,LDH,α-HBDH and IL-6 were significantly decreased at T2,3,the serum concentration of IL-10 was increased at T1-3,the changing rate of serum cTnI concentrations was decreased(P<0.05),and no significant change was found in the incidence of myocardial ischemia and ventricular premature beat and requirement for dopamine in group D(P>0.05).Conclusion Dexmedetomidine can attenuate the myocardial injury to some extent in pediatric patients undergoing living-related liver transplantation.

Objective To study the correlation between immune imbalance mediated by Th 17 and Treg cells and impaired lung function in patients with chronic obstructive pulmonary disease ( COPD ) . Methods Ninety-five patients with moderate or severe COPD , thirty-five smokers with normal lung function and thirty-one healthy non-smokers from Yinzhou People′s Hospital from January 2009 to December 2012 were recruited in this study .The percentages of circulating Th 17 and Treg cells in peripheral blood samples were determined by flow cytometry .The concentrations of cytokines in serum samples and supernatants of in-duced sputum samples were measured by enzyme-linked immunosorbent assay (ELISA).The expression of ROR-γt and Foxp3 at mRNA level in peripheral blood mononuclear cells ( PBMC) were determined by quan-titative real-time PCR.The potential association between ratios of Th 17/Treg cells and lung function was evaluated.Results Compared with smokers and healthy subjects , patients with moderate or severe COPD showed high levels of Th17 cells, IL-17A, IL-6 and IL-23, and an up-regulated expression of ROR-γt at transcriptional level .However, the percentage of Treg cells , IL-10 level and the expression of Foxp 3 at mRNA level were down-regulated in patients with COPD .The levels of cytokines in supernatants of induced sputum samples varied in the same way as that of Th 17/Treg in peripheral blood samples .The ratio of Th17 to Treg cells was negatively correlated with the values of forced vital capacity ( FVC) , forced expiratory vol-ume in one second (FEV1), and FEV1/FVC.Conclusion Th17/Treg cells imbalance was closely associ-ated with the deterioration of pulmonary function in patients with moderate or severe COPD .Th17/Treg cells imbalance might be involved in the progression of COPD .