OBJECTIVE:To investigate the effects of ligustrazine combined with N-acetylcysteine (NAC) on the peripheral pro-inflammatory cytokines and intestinal microflora of chronic stress rat. METHODS:Normal rats were included in normal control group,and chronic stress model rats were divided into model group,NAC group (60 mg/kg) and ligustrazine+NAC group (15 mg/kg+60 mg/kg),with 20 rats in each group. They were given relevant medicine intragastrically for 15 d. The general inspection of rats were recorded in each group. Level of IL-6,IL-1β,TNF-α and INF-γ in serum were detected,and intestinal microflora was measured. RESULTS:Compared with normal control group,chronic stress symptom of rats were found in model group,and level of IL-6,IL-1β,TNF-α and INF-γ in serum were enhanced;Escherichia coli and Enterococcus in intestine were increased,while Bacterium lacticum and Bacillus bifidus were decreased (P<0.05). Compared with model group,chronic stress symptom of rats were relieved in NAC group and ligustrazine+NAC group,and above indexes were all improved (P<0.05);ligustrazine+NAC group was better than NAC group(P<0.05). CONCLUSIONS:Ligustrazine+NAC can improve chronic stress of rats by decreas-ing pro-inflammatory cytokines and improving the intestinal microflora.

Aquaporins (AQP) are membrane channel proteins which facilitate water transport through cell membranes.AQP4 is abundantly expressed in brain which is located in endfeet of astrocytes adjacent to capillaries,neurons,and neuronal synapses.It has also been identified in neurons of the supraoptic and paraventricular nuclei of the hypothalamus.AQP are closely related to many central nervous system diseases.Researches have showed that AQP4 participates in regulating and controling process of cerebral edema that caused by brain trauma,cerebral ischemia,intracranial infection and mmour,etc.AQP4 antibody is specific biological markers in serum of patients with neuromyelitis,which helps diagnosis of neuromyelitis optica and multiple sclerosis,predicting prognosis and therapy of neuromyelitis optica.AQP4 has been shown to play roles in pathological and physiological process of neurodegenerative diseases,epilepsy,migraine.This article aims to review the distribution,function,influential factors of AQP4 and research development of AQP4 in common diseases of the central nervous system.

0.05 ), bacterial clearance were 100% and 90%, and adverse reaction rates were 10%(2/20)and 10.53 % ( 2/19 ), respectively. All those adverse reactions were mainly gastrointestinal tract reactions. The abnormality of laboratory inspections associated with trial drugs happened to 4 and 5 patients in two groups. The reactions were mostly light increase of aminotransferase without effect on therapy. CONCLUSION: Gatifloxacin is an antibiotic with large antimicrobial spectrum and can be recommended to treat light to moderate acute lower respiratory tract infections.

Objective An initial study on the cognition of the relationship between clinical and management staff for the purpose of improvements. Methods Questionnaires were issued to 38 clinical medical staff and 26 hospital management staff, regarding this relationship. Results Consensus is found between the two groups regarding clinical involvement in management, teamwork and exchange, and cognition on the competence and capability of the other party, P>0.05. On the other hand, however,cognitive differences are found regarding mutual penetration, power balance and implementation of policies and systems. Conclusions In-depth cooperation and understanding between the two groups and the mutual conversion of their behaviors and concepts are the cornerstones of an ideal relationship between clinical staff and management staff.

Bronchiolitis is a common respiratory infective disease in children, which is caused by viruses mostly. A view that bronchiolitis is a special pattern of manifestation of asthma is accepted widely. In the past few years, many agents appeared as a inducement from bronchiolitis to asthma, such as family histoty and atopy. At the same time, the life style of infants( feeding patterns,infective frequency ,environment exposure)plays a role in this process. Studying these factors will help doctors to detect people with high risk, and supply treatment earlier.

With the development of income per capita,"high personal medical expenditure" shows the wider of disease risk,which is resulted from the increasing medical payment and imperfect risk pooling.The former that is related with the excess delivery of advance medical technology and drug has been a dominating reason since 1980.And the imperfect risk pooling means the imperfect social medical insurance,of which the government should take charge.However,influenced by the reform policies of the economic systems,similar to all the transitional economies reducing the government role in healthcare financing,China has put an increasing emphasis on the payroll tax-based social insurance system and out-pockets payment,moving away from a predominantly general revenue-based financing system under the old command economy.In a conclusion,the Chinese Government should make clear the financing function,and replace fee-for-service(FFS) payment according to the distorted pricing system with aggregated pre-payment for patients covered by social insurance to mitigate the incentive for cost escalation.Meanwhile,the target of the new medical reform that should resolve the problem of high personal medical expenditure is demanding and perfecting the social medical insurance with wider risk pooling.

Objective: To explore the effect of the application of ICQ technology in constructing a harmonious environment for nursing staff.Method: The Internet,support from Tencent network,ICQ technology are employed to conduct on line communication among nursing staff.Result: the Working and interpersonal connection environment is remarkably improved by the application of ICQ technology,thus it is of great significance in constructing a harmonious relation among nursing staff and enhancing medical service quality.

OBJECTIVE: To establish HPLC fingerprint of Compound danshen tablets.METHODS: The separation was performed on Agilent Eclipse XDB-C18(150 mm?4.6 mm,5?m) column with mobile phase consisted of acetonitrile-0.04% phosphate (gradient elution) at flow rate of 1.0 mL?min-1.Detection wavelength was set at 272 nm and column temperature was 30℃.RESULTS: Established HPLC fingerprint was distinct and reproducible.There were 8 characteristic peaks among which No.5 characteristic peaks,No.6,No.7,No.8 came from HPLC fingerprint of Salvia miltiorrhiza and No.1 characteristic peaks,No.2,No.3,No.4 came from HPLC fingerprint of Panax notoginseng.CONCLUSION: The characteristic peaks of effective parts of Compound danshen tablet mainly come from S.miltiorrhiza.The study can be used for tracing and isolation of effective part of Compound danshen tablet and establishes basis for the further development of Chinese patent drugs.

Invasive aspergillosis (IA) is a major cause of morbidity and mortality in immunocompromised children.The purpose of the review was to provide epidemiological,clinical aspects,diagnosis and antifungal treatment options in the pediatric population.Diagnosis of IA remains a challenge as the clinical and imaging manifestations are not specific,and a histological diagnosis is often unfeasible,and blood culture shows low sensitivity rates.In recent years,great progress has been made in the detection of fungal biomarkers,which is mainly described in this paper.

OBJECTIVE:To discuss about the spot break for promoting technical skipping in pharmaceutical industry of China.METHODS:Based on imbalance of technical development,matrix of technical change skipping and cluster of innovation,the necessity and possibility of the spot break for promoting technical skipping in pharmaceutical industry of China were discussed.RESULTS & CONCLUSIONS:Because the spot or part break is the normal model for promoting technical skipping in history of technical change and innovation,so if pharmaceutical industry of China wants to get technical skipping,we have to promote the spot break.In the end of this paper,the suggestions how to get spot breaks in the skipping of pharmaceutical technical change and innovation in China are put forward.