OBJECTIVETo study the effects of Dipsacus total saponins on the ability of learning and memory and its mechanism of action.

METHODSForty rats were randomly divided into blank control group, model group, Dipsacus group and positive control group (n = 10), general situation of rats were observed, the ability of learning and memory of rats was tested by Square water maze, the activities of acetylcholinesterase (AChE)and choline acetyltransferase (ChAT) of hippocampus in rats were measured using double antibody sandwich method.

RESULTSDuring the period of treatment, general situation had no obvious change in model group, but general situation and the ability of activity were gradually improved in Dipsacus group and positive control group. Compared with blank control group, the swimming time was obviously prolonged and the number of mistakes was obviously increased at different time, the activity of AChE was significantly enhanced and the activity of ChAT was significantly decreased in model group. Compared with model group, the swimming time was obviously shortened and the number of mistakes was obviously reduced at different time, the activities of AChE were significantly decreased and the activities of ChAT were significantly enhanced in Dipsacus group and positive control group; Compared with positive control group, the swimming time and the number of mistakes at different time and the activities of AChE and ChAT had no significant difference in Dipsacus group.

CONCLUSIONDipsacus total saponins can improve the ability of learning and memory in Alzheimer' s disease(AD) rats, its mechanism of 'action may be related to regulating ACh metabolism of hippocampus.

Acetylcholine ; metabolism ; Acetylcholinesterase ; metabolism ; Alzheimer Disease ; drug therapy ; physiopathology ; Animals ; Choline O-Acetyltransferase ; metabolism ; Dipsacaceae ; chemistry ; Disease Models, Animal ; Hippocampus ; drug effects ; Learning ; drug effects ; Memory ; drug effects ; Rats ; Saponins ; pharmacology

The cause of dilated cardiomyopthy (DCM) has not been elucidated yet .The poor prognosis and lack of specific and effective treatment became a serious health problem .Therefore to establish animal models of DCM to study the pathogenesis and treatment is necessary and urgent .At present the molding method of DCM mainly includes drug induction, immunological induction and molecular biology , etc.This article reviews the mature and commonly used establishing methods and mechanism of DCM models .

In this paper,we summarized nursing care of 9 patients with cytokine release syndrome after receiving the chimeric antigen receptor T cells immunotherapy.Key points of nursing care were as follows:comfortable and effective antipyresis,preventive rehydration,multiple methods of blood oxygen saturation improvement,iatrogenic pressure ulcer prevention,frequent vital signs monitor,close observation of level of cytokine and inflammatory factors,timely and accurate medication,formulating personalized nursing plan according to patients' condition,close collaboration between doctors and nurses.At last,8 patients recovered and were discharged,and 1 case died due to multiple organ dysfunction syndrome.

Based on the research goal of "traditional Chinese medicine standards lead in the international standard-setting", scientific strategies for the elaboration of traditional Chinese medicine (TCM) comprehensive quality standard system were introduced. TCM is a complex multi-component system which was used under the guidance of traditional Chinese medical theory. The present paper has put forward the basic principle of " deep research and simplified standard" to construct the quality standards of TCM. " Deep research" refers to systematic, thorough investigations on active constituents and biological mechanisms. On the basis of deep research, "simplified standard" means to establish a scientific and feasible standard, which should be practical and less complicated, to control the quality of Chinese herbs. Three key issues related to the elaboration of the quality standards are suggested. For reference substances, we propose the research methodology using reference extract to develop the qualitative and quantitative determination methods. Compared with that of using reference compounds, the preparation of reference extract is more accessible, economical and practical. Moreover, compared with reference crude drugs, the reference extract showed better batch-to-batch consistency. For identification, in addition to conventional methods, high performance liquid chromatography (HPLC), chemical fingerprints and characteristic chromatogram are proposed, in which more major marker compounds are monitored. And then LC-MS technique is employed to comprehensively analyze and characterize the peaks in the fingerprint. For multi-component quantification, the method of single standard to determine multi-components (SSDMC) is suggested, providing solutions for the lack of reference standards in quality evaluation. The SSDMC method uses a single reference standard to simultaneously determine the content of multiple compounds. In general, the integrate quality control standard of TCM is established based on combining innovative technology with practical applications.
Chemistry Techniques, Analytical ; Medicine, Chinese Traditional ; standards ; Quality Control ; Reference Standards

Keratoconus is a progressive, non - inflammatory corneal thinning disorder that produces a unique spectrum of change in the surface curvature of the cornea. The suspected factors associated with the cause of keratoconus include genetic mechanisms, familial associations, allergy, atopy and so on, but environmental factors, such as abnormal rubbing, contact lens wearing and ultraviolet radiation may be easily ignored. Although it is commonly thought that keratoconus is a result of various pathological progress mediated by multiple factors, those chronic trauma could play important roles in the pathological progress. The classic definition of keratoconus is still referred as a non - inflammation disorder with malnutrition of keratectasia, more and more clinical evidences and experimental results that may refute this concept. It may be due to the long-term persistent chronic trauma resulted from eye rubbing, contact lens or ultraviolet radiation. Under this conditions, the increased release of inflammatory mediators and matrix metalloproteinases may accelerate keratocyte apoposis and enzyme metabolism disorders, with the result of matrix cell lost, tensile strength decreased and keratectasia. Here, for some papers known about the relationship between chronic cornea trauma and keratoconus were reviewed and discussed its possible causal relationship.

Objective To approach therapy of acute lymphoblastic leukemia(ALL) complicating acute respiratory distress syndrome (ARDS) in children.Methods The therapy of seven children diagnosed as ALL complicating ARDS was analyzed, who were treated by anti-infection, methyllprednisolone, ambroxol and constant positive airway pressure (CPAP) assisted ventilation.Results Six cases were recovery and one was death. The cure rate was 85.7%. Conclusion The cure rate is high, when employing combined therapy to treat ALL complicating ARDS.

Objectives:To observe the role of nutritional support in patients after liver transplantation. Methods:Nutritional support was used in three patients after orthotopic liver transplantation(OLT).Total parenteral nutrition(TPN) was administered since the second day after the operation,the combination of enteral nutrition(EN) and parenteral nutrition(PN) was followed and then total enteral nutrition(TEN) was adopted.After that,oral intake of food was restored. Results:Postoperative patients were restored well. Conclusions:The supply of nutrition for patients after liver transplantation should be TPN→PN+EN→TEN,and then gradually increased.Once the gastroenteric functions of patients recover it is advisable to start EN as soon as possible.

Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Carcinoma, Non-Small-Cell Lung ; diagnostic imaging ; drug therapy ; Female ; Fluorodeoxyglucose F18 ; Humans ; Lung Neoplasms ; diagnostic imaging ; drug therapy ; Male ; Middle Aged ; Positron-Emission Tomography ; Small Cell Lung Carcinoma ; diagnostic imaging ; drug therapy ; Tomography, X-Ray Computed

OBJECTIVETo Study the effect of anti-aging and its mechanism of total saponins of Wu-He Dipsacus asper on skin of mice-aging model.

METHODSForty-eight mice were randomly divided into blank control group, model group, low-Dipsacus group, medium-Dipsacus group, high-Dipsacus group and positive control group( n = 8) . The mouse model of skin aging was established by nape subcutaneous injection of 5% D-galactose (0.025 mL/(g · d)), the mouse of low-Dipsacus group, medium-Dipsacus group, high-Dipsacus group were administered with total saponins of Wu-He Dipsacus asper (50 ml/(kg · d), 100 mL/(kg · d), 200 mL/(kg · d)), the mice of the positive control group were administered with vitamin E(50 mg/(kg · d)) for 42 d. The content of hydroxyproline (HYP) and lipofuscin (LF) were measured in skin of each group mice, the activity of catalase (CAT) glutathione peroxidase ( GSH-Px) superoxide dismutase (SOD) and the content of malondi- aldehyde (MDA) were determined in serum and skin of each group mice.

RESULTSCompared with blank control group, the content of HYP decreased significantly and the content of LF increased significantly in skin, the activities of CAT, GSH-Px and SOD decreased significantly and the content of MDA increased significantly in serum and skin of model group; Compared with model group, the content of HYP increased significantly and the content of LF decreased significantly in skin, the activities of CAT, GSH-Px and SOD enhanced significantly and the con- tent of MDA decreased significantly in serum and skin of low-Dipsacus group, medium-Dipsacus group, high-Dipsacus group and positive control group; Compared with low-Dipsacus group, the content of HYP increased significantly and the content of LF decreased significantly in skin, the activities of CAT, GSH-Px and SOD enhanced significantly and the content of MDA decreased significantly in serum and skin of high-Dipsacus group and positive control group; The activity of SOD in serum and skin had a significant positive correlation with the content of HYP, and a significant negative correlation with LF in skin.

CONCLUSIONTotal saponins of Wu-He Dipsacus asper have obvious effect of anti-agng on skin of mouse-aging model , its mechanism is closely related to oxidative damage.

Animals ; Dipsacaceae ; chemistry ; Disease Models, Animal ; Mice ; Oxidative Stress ; Saponins ; pharmacology ; Skin Aging ; drug effects

Objective To study the effect of rapamycin on the balance of T helper cell subsets in rats with pulmonary fibrosis. Methods Fifteen male Sprague-Dawley (SD) rats were randomly divided into control group, model group and rapamycin group, with 5 rats in each group. Pulmonary fibrosis model was reproduced by using the method of intratracheal instillation of bleomycin (5 mg/kg). Control group was treated by intratracheal instillation of saline (1.25 mL/kg) to obtain the negative control. The rats of the rapamycin group were given rapamycin (1 mg·kg-1·d-1) by gastric perfusion for consecutive 10 days beginning on the 3rd day after intratracheal instillation of bleomycin. On the 28th day all rats were sacrificed, and the peripheral blood and the lung tissues were harvested. The lung tissue was observed. And the severity of pulmonary fibrosis in rats was assessed by Ashcroft score. The lung tissues were performed using immunohistochemical staining to detect the expression ofγ-interferon (IFN-γ) and interleukins (IL-4, IL-17). Flow cytometry was used to detect the percentages of CD4+ T cells and CD8+ T cells. Results The severity of pulmonary fibrosis was improved in rats of rapamycin group compared with that of model group, and the Ashcroft score was decreased (2.92±0.64 vs. 5.76±1.76, F = 16.276, P = 0.080). The expression levels of IL-4 and IL-17 (A value) in model group were the highest (4 789.0±1 014.6, 19 139.0±2 433.3), followed by those of the rapamycin group (3 547.0±953.8, 10 380.0±2 352.4), and the least was found in the control group (1 627.0±914.8, 4 419.0±923.6). The expression levels of IFN-γ (A value) in control group were the highest (9 956.0±1 172.6), followed by those of the rapamycin group (7 487.0±998.4), and the least was found in the model group (6 054.0±1 045.2). There were significantly differences in above parameters among three groups (all P<0.01). Compared with the control group and model group, the percentages of CD4+CD25+Foxp3+T cells in CD4+CD25+, CD4+cells, and lymphocytes were significantly increased in rapamycin group [(57.36±8.84)% vs. (41.28±5.91)%, (34.52±4.56)%; (4.77±0.48)% vs. (3.15±0.37)%, (3.14±0.28)%;(1.97±0.22)%vs. (1.24±0.17)%, (1.44±0.21)%, all P<0.05], and the percentages of CD8+CD25+Foxp3+T cells in CD8+CD25+, CD8+cells, and lymphocytes were also significantly increased in rapamycin group [(73.92±7.69)% vs. (33.44±4.46)%, (49.14±11.38)%; (1.73±1.05)% vs. (0.46±0.15)%, (0.71±0.42)%;(0.31±0.20)% vs. (0.09±0.04)%, (0.14±0.09)%, all P < 0.05]. The percentage of CD8+CD25+Foxp3+ T cells in CD8+CD25+in model group was significantly higher than that in control group [(49.14±11.38)% vs. (33.44±4.46)%, P < 0.05]. Conclusions T helper cell subsets are imbalanced in pulmonary fibrosis rats. Rapamycin can prevent bleomycin-induced pulmonary fibrosis, and its antifibrotic effect maybe the promotion of proliferation and function of regulatory T cells and imbalance regulation of T helper cell subsets.