Neonatal bacterial meningitis is difficult to diagnose at the onset,with atypical symptoms and high mortality,those who survive would have neurological complications.Early diagnosis and standard treatment are very crucial.European Society for Clinical Microbiology and Infectious Diseases published a guideline to the diagnosis and treatment of acute bacterial meningitis.In combination with the latest research results and the guideline,this article aims to provide guidance in diagnosis and treatment of neonatal bacterial meningitis.

Gastrointestinal Stromal Tumors ; diagnosis ; epidemiology ; surgery ; Humans ; Stomach ; pathology

Colorectal Neoplasms ; pathology ; surgery ; Humans ; Liver Neoplasms ; secondary ; surgery ; Neck Dissection

Accurate preoperative staging is imperative in the treatment of gastric cancer since diseases in different stages mandate different management strategies. Multi-disciplinary therapy is important for gastric cancer. Endoscopic treatment has become the preferred treatment for patients with early gastric cancer. However, only when multiple disciplines is involved in the decision-making process, appropriate patients can be selected and benefit from the minimally invasive treatment. In China, 90% of gastric cancers are at advanced stage at diagnosis, which are associated with worse prognosis compared to early gastric cancer. The optimal treatment depends on the multi-disciplinary therapy including surgery, chemotherapy, radiotherapy, and molecular targeted therapy.
Combined Modality Therapy ; Humans ; Stomach Neoplasms ; therapy

Animals ; Cadmium Compounds ; toxicity ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Cricetinae ; Cricetulus ; Fibroblasts ; drug effects ; metabolism ; Lactate Dehydrogenases ; metabolism ; Lung ; cytology ; Malondialdehyde ; metabolism ; Quantum Dots

Objective To screen the conservative,stable and specific DNA signature sequence in the plasmid of Yersinia pestis.Methods Specific validation trials and stability of the qualification test were carried out to 40 strains of Yersinia pestis,47 strains of non-Yersinia pestis of home and wild types of rodent in Yunnan,by using 32 DNA sequences derived from Yersinia pestis in the plasmid and conventional PCR technology,and Yersinia pestis vaccine strain EV76 as a positive control.Results Four pairs of relatively conservative,stable and specific DNA marker genes were screened:YPMT1.05c,YPMT1.03c,YPMT1.42 and YPMT1.04c.Conclusions The 4 pairs of Yersinia pestis DNA signature sequences can be used for rapid diagnosis of plague.

Objective This study aimed to establish an anatomic basis for the microscopic surgical anatomy of the cavemous sinus via the extended transsphenoidal approach.Methods Simulated surgery via extended transsphenoidal ap-proach was performed on seven adult cadaver heads with red--colored latex injected arteries.The cavernous segment of the ICA and its branch vessels and its relationship with cranial nerve were exposed and its anatomic parameters were measured under microscope.Results The tuberculum sellae,clivus,sellar base,ICA prominence,and optic nerve prominence are the important bone landmarks to define the surgical area.The average of the extent of bone removal of extended transsphe-noidal approach is 37.6 mm(range:28.7 mm～44.0 mm).Conclusions The bone removal from sellar base to the media edge of the foramen rotundum and over the ICA prominence can effectively expose the entire unilateral cavernous sinus.The microscopic cavernous sinus surgery via the extended transsphenoidal approach is an optimal surgical approach for the le-sions that invade the cavernous sinus from sella.

As the development of technology in Neonatal Intensive Care Unit,the neurology outcome of premature has become a critical issue for their future life quality.Early electroencephalogram (EEG) test is a noninvasive and convenient method to monitor brain function.Nowadays,EEG monitoring has been used to evaluate the maturity of background activity,to detect subclinical seizures and to predict future neurological outcomes.The guideline of American clinical neurophysiology society in 2011,and the guideline of French clinical neurophysiology society in 2015 have made recommendations for the indication,timing and duration of EEG monitoring.Interpret EEG needs both skill and experience,it is hard for clinicians to master the skill in a short time.Hence,despite the advantages of EEG monitoring,the application of EEG among premature is limited.The amplitude-integrated EEG and other quantitative EEG monitoring method have become the latest research hotspot for evaluation of preterm brain function.In order to improve the recognition of EEG in the area of preterm neurologic evaluation,now the latest researches are reviewed.

Objective To improve the recognition of dermatomyositis (DM) complicated with intracranial neoplasma and to explore its pathogenesis aimed to collect the experience of diagnosis and treatment. Methods Twelve DM patients complicated with intracranial neoplasma were analysed and the literature of recent 23 years were reviewed. Results DM complicated with intracranial neoplasma was associated with the p53 genic mutation and it was rare because of blood-brain barrier and scanty of lymphocytes in brain tissue. Correct diagnosis depended on clinical manifestations and the computerized tomography or the magnetic resonance imaging of brain. Glucocorticoid was the first choice treatment.However, removal the neoplasma was necessary. Conclusion DM complicated with intracranial neoplasma is rare. Clinicians should make the diagnosis promptly and chosen the appropriate treatment.

Objective To investigate the association between genetic polymorphisms of exon3 of nicotinic acetylcholine receptor ?4 subunit (CHRNA4) and sporadic Alzheimer's disease(SAD).Methods In 23 SAD patients and 30 people of normal control, exon3 of CHRNA4 was screened by PCR-DGGE and DNA sequencing.Results Three new genetic polymorphisms were found in the exon3 of CHRNA4: C104T, A136G, G169A.The frequnces of these three genes in SAD group were 35%,46%,61% respectively and in control group were 13%,13%,33% respectively. There were significant difference between two groups (all P