OBJECTIVE:To discuss the choice and the route for the realization of brand strategy feasible to Chinese pharmaceutical industry(CPI)in the current and future competition environment.METHODS:Reasons for the lack of influential brands were analyzed based on the current practical situation of CPI,then some countermeasures were put forward.RESULTS&CONCLUSIONS:Knowing the importance of brands and strengthening brands construction are the key issues for CPI to consider in the market competition,however,establishing national or even international brands is the long-term target of the future development of CPI.

Objective To study the seasonal change of antibodies levels in the serum of asthma patients and Der P1 concentration in the dust samples collected from their bedrooms. Methods 36 asthma patients who were allergic to mites were treated as the patients group,21 male and 15 female,aged 43 years of average age. 20 healthy individuals were treated as the control group,11 male and 9 female,with the same age as the patients group. In September and December in 2005,and Match and June in 2006,the dust samples in the bedrooms of patients and the venous blood were collected,the samples of the control were collected only in September. Der P1 concentrations in dust samples and the levels of total IgE,mite specific IgE (s-IgE),s-IgG1,s-IgG2,s-IgG3 and s-IgG4 were detected by ELISA assay. Results In autumn,the levels of total IgE,s-IgE,s-IgG1,s-IgG2,s-IgG4 and the Der P 1 concentrations in patients were significant higher compared with the control group (P

Objectives To study the effect of Lactulose oral solution combined with polyethylene glycol electrolyte powder on the intestinal cleanness.Methods One hundred and twenty patients undergoing electronic colonoscopy were divided into the experiment group and the control group,60 cases in each group.The experiment group orally took Lactulose oral solution and polyethylene glycol electrolyte powder 1d before the check,while the control group polyethylene glycol electrolyte powder 1d before the check.The two groups were compared in terms of intestinal cleanness and adverse reactions.Results The experiment group was significantly better than the control group in intestinal cleanness(P>0.05)and there were no significant difference has in adverse reactions(P>0.05).Conclusion The combined use of polyethylene glycol electrolyte powder and Lactulose Oral Solution may improve the intestinal cleanness and reduce the incidence of adverse reactions.

OBJECTIVE:To explore a comprehensive drug quality evaluation method so as to provide constructive suggestions for the production and quality inspection of drugs.METHODS:The quality inspection data of 198 batches of flupentixol and melitracen tablets collected automatically by Laboratory Information Management System(LIMS) in Guangdong institute for drug control in 2008 were analyzed statistically,and the drug quality indexes were computed and the drug quality was analyzed comprehensively.RESULTS:Of all the quality items,the content uniformity showed the maximum impact on the quality of the tablets,followed by the content determination item,with disintegration time item showing the least impact on the quality of the tablets.CONCLUSIONS:LIMS is a powerful tool in collecting large quantities of drug data,and the quality index serves as an effective means for the evaluation of drug quality.

In this paper, the Fourier transform based minimum mean square error (FT-based MMSE) method is used to calculate the regional cerebral blood volume (rCBV) in magnetic resonance (MR) perfusion imaging, and the method is improved to handle the existing noise in the imaging process. In the experiments with signal-to-noise ratio (SNR) of 50 dB, the rCBV values were compared with the results using MMSE method. The effects of different SNRs on the estimation of rCBV were analyzed. The experimental results showed that MMSE was a simple way to filter the measurement noise, and could calculate rCBV accurately. Compared with other existing methods, the present method is not sensitive to environment, and furthermore, it is suitable to deal with the perfusion images acquired from the environment with larger SNR.
Blood Volume ; Brain ; blood supply ; Fourier Analysis ; Humans ; Magnetic Resonance Angiography

Acupuncture Points ; Acupuncture Therapy ; Adult ; Combined Modality Therapy ; Female ; Humans ; Intervertebral Disc Displacement ; therapy ; Lumbar Vertebrae ; pathology ; Male ; Middle Aged ; Musculoskeletal Manipulations ; Prolapse

Gadolinium DTPA ; Humans ; Magnetic Resonance Imaging ; Non-alcoholic Fatty Liver Disease

Objective To analyze the interactions in lectures conducted by young teachers in medical institutions and investigate the way of improving their ability. Methods We analyzed the teaching plans and the actual performance of 31 teachers who enter the final of the 16th competition of young teacher's basic skills, 2011 and observed the lectures of another 15 randomly selected teach-ers, focusing on the interactions and the effects in the lectures. Results Among the 31 teachers, 19 (which is 61.29% of the total) teachers designed and implemented interactive teaching; 6 (which is 40.00% of the total) teachers adopted interactive teaching in daily teaching. Among the 40 young teachers included in the research , 27 ( which is 54 . 35% of the total ) teachers employ interactive teaching. The way of performing interactive teaching in all above situations is answering questions. Conclusions Interactive teaching is commonly used in lectures by young teachers in medical insti-tutions. However, the method is simple and the quality needs to be improved.

Objective To evaluate the relationship between the T1 relaxation times using gadoxetic acid-enhanced MRI with the organic anion transporter (OATP) and multi-drug resistance protein (MRP) values on colorectal liver metastasis and around normal liver parenchyma.Methods The perspective study included in fifteen patients with liver metastasis from colorectal cancer,who were confirmed colorectal cancer by colonoscopy,liver metastasis by CT scanning and would be underwent liver metastasis surgery.The lesion on the 15 patients was all single.The patients were performed plain scanning,diffusion weighted imaging and dynamic enhancement MRI examination using gadoxetic acid.Besides,for T1 mapping,a dual flip angle three-dimensional gradient echo sequence was performed before and at 20 minutes after gadoxetic acid administration.The reduction rate of T1 relaxation time (ΔT) (before and after gadoxetic-acid administration) of colorectal liver metastasis and around normal liver parenchyma was calculated.The specimens of hepatic metastases and around normal liver parenchyma were obtained during the colorectal liver metastasis operation.After that,they were analyzed by Western-blot and the values on OATP1,OATP8 and MRP2,MRP3 were recorded.The values on OATP1,OATP8 and MRP2,MRP3 and ΔT on hepatic metastases and around normal liver parenchyma were compared with t analysis.The correlation between the sum of the values (hepatic metastases adding around normal liver parenchyma) on OATPI,OATPS,MRP2,MRP3 with T1 relaxation times were calculated by Spearman.Results The scanning images on colorectal liver metastasis using gadoxetic acid MRI examination were as follows:relatively low signal intensity on T1WI; relatively higher signal intensity on T2WI; showing surrounding moderate enhancement on arterial phase and low signal intensity on portal venous phase,delay phase and hepatobililary phase.The values on OATP1,OATP8,MRP2,MRP3 and AT on the hepatic metastases were(0.21±0.09),(0.34±0.21),(0.41±0.22),(0.26±0.14)ng/ml and (33±9)％,respectively.The values on OATP1,OATP8,MRP2,MRP3 and ΔT around normal liver parenchyma were (0.46±0.24),(0.67±0.35),(0.22±0.13),(0.13±0.06) ng/ml and(68±9)％ (t values were 36.37,58.89,24.56,22.52 and 57.16;P＜0.05).The values on patients on OATP1,OATP8,MRP2 and MRP3 [(0.67±0.31),(1.00±0.37),(0.63±0.23),(0.40±0.13)ng/ml,respectively] showed relationship with the values on patients on ΔT(51±9)％ (r values were 0.491,0.432,-0.414 and-0.371;P values were 0.006,0.017,0.023 and 0.040).Conclusion The sum of the values (the hepatic metastases adding around normal liver parenchyma) on OATP1,OATP8,MRP2 and MRP3 showed correlation with the sum of values on the ΔT using gadoxetie acid-enhanced MRI examination,which explained relationship with the quantity of gadoxetic acid absorbed by hepatocyte and the T1 relaxation time on molecular level.

The primary structure of TNK-tissue plasminogen activator (TNK-tPA) was characterized using liquid chromatography-mass spectrometry (LC-MS). Firstly, the molecular mass of deglycosylated protein was measured. Then peptide mass mapping and MS/MS of the reduced, alkylated and trypsin-digested sample were tested and analyzed so as to verify its amino acid sequence and identify post-translational modifications. Results show that the amino acid sequence was consistent with designed structure; about 5% of M207 was oxidized; T61 was fucosylated with -80% occupancy; N103, N448 and N184 (-15% occupancy) were glycosylated with complex-type oligosaccharides. LC-MS coupled with proper sample pretreatment is approved to be a rapid and powerful approach to characterize the primary structure of TNK-tPA.