BACKGROUND:Although incidents of organophosphorus poisoning-induced delayed neuropathy (OPIDN) have been documented for over a century, the molecular mechanisms underlying the axonopathy remain poorly understood. Therefore, OPIDN treatment has been increasingly concerned. OBJECTIVE:To construct the OPIDN hen model induced by triorthocresyl phosphate (TOCP) and to explore the effect of phenylmethylsulfonyl fluoride (PMSF) intervention. METHODS:Adult hens were randomly divided into four groups: two TOCP groups, a PMSF group and a control group. TOCP groups were treated with TOCP by gavage at a single dosage of 1 000 mg/kg and 750 mg/kg respectively; control group was given an equivalent volume of saline by gavage while hens in the PMSF group were subcutaneously injected with 40 mg/kg PMSF 24 hours after 1 000 mg/kg TOCP injection. OPIDN neurological signs were assessed by a six-point graded scale. The changes of the hen weight were recorded. The hens were kiled on day 5 and 21 post-dosing. The samples were cut into 50 nm thick sections and examined by transmission electron microscopy. RESULTS AND CONCLUSION:OPIDN neurological signs such as abnormal gaits progressed in severity with time (P < 0.05), and the hen weight was significantly decreased in TOCP groups (P < 0.05). However, no clinical signs of delayed neurotoxicity were observed in hens of the PMSF group and the control group during the experiment period. The mild mitochondrial sweling and the fragmentation of microfilament and microtubule arrangement in axons were observed on day 5 post-dosing, leaving the other organeles remained unchanged. On day 21, neuronal degeneration was apparent, including sweling of endoplasmic reticulum, abnormal change of mitochondria, and disordered arrangement of cytoskeleton. The optimal dose of TOCP was 1 000 mg/kg. Experimental findings indicate that, OPIDN hen model induced by TOCP and PMSF intervention hen model were successfuly constructed. PMSF intervention significantly improved the pathologic changes and clinical symptoms of OPIDN induced by TOCP in hens.

AIM: To immediately observe the complication of corneal flap after LASIK surgery.
METHODS:A retrospective case series were studies. Totally 2 040 cases ( 4 080 eyes ) from January 2010 to October 2012 in our hospital were collected, the corneal flap was observed using lamp microscope after LASIK within 30min. Corneal flap dislocation, corneal flap strial and intraface debris were examined after operation, the effective treatment and controlled measure should be taken for these complications.
RESULTS: Postoperative complications were corneal flap dislocation 102 eyes (2. 5%), corneal flapstriae 95 eyes (2. 33%), interface debris 105 eyes (2.57%). No failure case was seen. There had no corneal flap-related complications, which seriously impact the visual quality after the surgery.
CONCLUSION: Carefully postoperative examination at the first - time is an effective way to manage some complications of post - LASIK. Thus promoting the diagnosis and treatment of post-LASIK complications.

BACKGROUND:Although incidents of organophosphate-induced delayed neurotoxicity have been documented for over a century, the molecular mechanisms underlying the axonopathy remain poorly understood.
OBJECTIVE:To discuss the effects of tri-ortho-cresyl phosphate (TOCP) on homeostasis of the glutamate-glutamine cycle and the expression of key enzymes in the brains of hens.
METHODS:Twenty-four adult hens were randomly divided into three groups (n=8). TOCP group was treated with TOCP by gavage at a single dosage of 1 000 mg/kg, and control group was given an equivalent volume vehicle by gavage, while hens in the phenylmethylsulfonyl fluoride (PMSF)+TOCP group were subcutaneously injected with 40 mg/kg PMSF fol owed by 1 000 mg/kg TOCP 24 hours later. The hens were kil ed on days 5 and 21 post-dosing. The brains were taken out quickly and preserved in a-80℃deep freezer. ELISA was used to determination the content of glutamine synthetase and glutaminase and the activity of glutamine synthetase. Corresponding kits were used to measure the level of glutamate and glutamine. Fluo3-AM was used to measure cytosolic calcium concentration. RESULTS AND CONCLUSION:The activity and content of glutamine synthetase and the concentration of glutamine were down-regulated, while the concentrations of the intracellular Ca2+and glutamate were up-regulated in the early stage after TOCP administration. It is also suggested that the downregulated expression of glutamine synthetase may be associated with organophosphate-induced delayed neurotoxicity through the disruption of homeostasis of the glutamate-glutamine cycle and cytosolic calcium concentration.

In the paper,in vitro degradation of DL polylactic acid (PDLLA) film was studied.Regulation and tendency of degradation were showed by molecular weight regulator and monomer ration mass lossing.Degradation mechanism was discussed in light.It was thought that degradation of PDLLA in vitro was a simple hydrolysis reaction.The hydrolysis reaction was self catalytic reaction caused by terminal carboxyl group.

Lactide一glycolide copolymerization with stannous octanoate as an initiator and dedecanolas regulator was carried out.The effect of reaction condition such as reaction time,temperature, initiatorconcentration, regulator and monomer ratbonpolymerization conversion,molecular weight and its distrbu-tion were investigated.The obtained results show that the finest reaction condition are initiator 0. 075%,ternperature 170℃ and reaction time 6hr.At this reaction condition,both molecular weight and polymer-ization conversion obtained are vecy high.

Objective To evaluate the effect and the best concentration of Rg 3 combined with delaying tamoxifen resistance in breast cancer .Methods The tamoxifen-resistant(TAM-R)cell line with TAM was es-tablished.TAM-R cell line and WT -MCF-7 cell line were cultured in TAM at different concentrations and then detected the growth of cells .WT-MCF-7 cell line was cultured medium in TAM which contained Rg 3 at different levels .The growing situation of cells was detected with MTT method .Results Compared with wild -type WT-MCF-7 cells,TAM-R showed higher curves when they were both at TAM treatment .Rg3 could be associated with delaying the emergency of tamoxifen resistance and the best concentration was 1 ×10 -8 mol/L and 5 ×10 -8 mol/L.Conclusion It might be useful to reduce the tamoxifen resistance incidence by clinically com-bined Rg3 with TAM treatment .

AIM:To compare 2 environments , the subrenal capsule and oral submucosa , for producing well-formed teeth from mouse tooth germs and for exploring the ideal environment for tooth regeneration .METHODS: Two groups were set up .Group A was transplanted with the mouse embronic day ( ED) 14.5 first mandibular molar tooth germs into the subrenal capsule , while group B was transplanted with the ED 14.5 first mandibular molar tooth germs into the oral submucosa.After 3 weeks and 4 weeks, the host mice were sacrificed, and the transplanted explants were evaluated with morphologic observation , histological structures , hardness and elastictic modulus tests , and chemical compositions .RE-SULTS:(1) The explants isolated from both environments showed the tooth-like structures, but as to the group B, the crown was smaller, and the shape of the cusps was not significant .(2) HE staining showed that the dentin and enamel in group A were thicker than those in group B in which the ameloblasts and odontoblasts were differentiated not very well .(3) In the test of enamel hardness , only the hardness of 4 weeks in group B was lower than normal mouse tooth .In the test of enamel modulus , the elastic modulus of enamel in 3 weeks of group A was slightly lower than normal mouse tooth , but the difference was not significant .The elastic modulus of enamel in 4 weeks of group A and group B was significantly lower than normal mouse tooth and 3 weeks of group B .The hardness and elastic modulus of dentin in 3 groups was not significant . (4) Raman spectroscopy showed 2 groups grew in harmony in general , they all had the largest peak in the point of 961 cm-1 , but the 3 weeks of group B had an obvious peak in the point of 2 947 cm-1 .CONCLUSION:For the development of ED14.5 tooth germs, we obtain almost the whole tooth in subrenal capsule transplantation after 3 or 4 weeks.The buccal submucosa environment still has a certain influence on the tooth germ development , although there are some differences about the tooth development between this environment and subrenal capsule environment .

In view of the difficulties in the practice of innovation education,Harbin Medical University identified the education objective,cultivated innovative education concept and implemented this concept by launching innovative ability training projects for college students,establishing innovation base and emphasizing on information technology and network based innovative education practice.satisfactory education effect was achieved.

Acinetobacter baumannii ; drug effects ; isolation & purification ; Drug Resistance, Multiple, Bacterial ; Female ; Humans ; Infant, Extremely Low Birth Weight ; Infant, Newborn ; Pneumonia, Ventilator-Associated ; etiology

The points, Neiguan (PC 6)-Gongsun (SP 4), Houxi (SI 3)-Shenmai (BL 62), Lieque (LU 7)-Zhaohai (KI 6), or Waiguan (TE 5)-Zulinqi (GB 41), were selected according to the eight magic turtle techniques and some acupoints were added on the basis of syndrome differentiation. Thirty obesity patients were treated. Marked effectiveness occurred in 6 cases, effectiveness in 21 cases and ineffectiveness in 3 cases, with a total effective rate of 90.0%.