Integrin ?V?3, one of the major members of the integrin family, plays a pivotal role in tumor angiogenesis because it mediates the cell-extracellular matrix and cell to cell adhesion as well as controls cells proliferation, differentiation, cellular modality and motility.Due to restricted high-expressions in tumors, integrin ?V?3, is considered a suitable targeting receptor for tumor diagnosis.In the past decade, several molecular imaging tracers for integrin ?V?3, have been used to noninvasively visualize its expression in tumor tissues and angiogenic blood vessels through the usage of nuclear imaging, nuclear magnetic resonance imaging, ultrasound, and optical imaging.Noninvasive detection of integrin ?V?3, expression can potentially be used for tumor diagnosis and prognosis.The current imaging probes used to target integrin ?V?3, through usage of different technologies are briefly recapitulated in this review.

Objective: To observe the clinical efficacy of electroacupuncture (EA) with different frequencies in treating migraine.Methods: Ninety patients with migraine were divided into a low frequency electroacupuncture (LF-EA) group, a high frequency electroacupuncture (HF-EA) group and a variable frequency electroacupuncture (VF-EA) group by the random number table method. Shuaigu (GB 8), Hegu (LI 4), Waiguan (TE 5), Taichong (LR 3), Taiyang (EX-HN 5), Fengchi (GB 20) and Ashi points were selected for all three groups. After achieving needling sensation (Deqi), the LF-EA group received 2 Hz continuous wave EA stimulation; the HF-EA group received 100 Hz continuous wave EA stimulation, and the VF-EA group received 2 Hz/100 Hz sparse-dense wave EA stimulation. The EA stimulation lasted for 30 min in all the three groups, once a day, 10 times as a course, and the clinical efficacy was evaluated after 2 consecutive courses of treatment. Results: After treatment, the total effective rates in the LF-EA group, HF-EA group and VF-EA group were 77.7％, 83.3％ and 93.3％, respectively. The difference in total effective rate between the LF-EA and HF-EA groups was not statistically significant (P>0.05), but the total effective rate was significantly higher in the VF-EA group than in the LF-EA and HF-EA groups (both P<0.01). After treatment, the headache scores significantly decreased in all three groups (all P<0.01). The difference in headache score between the LF-EA and HF-EA groups was not statistically significant (P>0.05), while the score was significantly lower in the VF-EA group than in the LF-EA and HF-EA groups (both P<0.01). Conclusion: VF-EA (2 Hz/100 Hz), compared with LF-EA (2 Hz) and HF-EA (100 Hz), shows superior clinical efficacy in treating migraine.

Child ; Humans ; Intestinal Polyps ; genetics ; Molecular Biology

OBJECTIVE:To summarizethe application of polypropylene repair material in treating inguinal hernia.METHODS:Papers regarding polypropylene repair material in treating inguinal hernia published in VIP data between January 1998 and October 2009 were researched using key words of "polypropylene,inguinal hernia,and complication".Inclusion criteria Repairing inguinal hernia using polypropylene materials.Experimental results concerning related complications.Exclusion criteria:repetitive study and relatively old literature.A total of 17 documents were included in this study.RESULTS:Organic polymeric materials were clinical utilized in repairing inguinal hernia at the end of 1950s.The polypropylene patch was knitted by slngle fiber or multi-strands fibers of polypropylene,and exhibited single layer network structures,which is preferred material for repairing abdominal-wall defect.Compared with other unabsorble materials,the polypropylene patch had following advantage:obviously stimulating fibroplasias; easy permeated by fibrous tissues and embedded into tissues; maintaining highly tensile strength following implantation; and relative low cost.Few material-related complications occurred following repairing,mainly presented with infection,serofiuid swelling,ankylenteron,intestinal leakage,material displacement,as well as material shrinkage.CONCLUSION:Polypropylene material is an ideal repair material due to good histocompatibility,few rejections,and anti-infection capability.It leads to easy operation,small trauma,fast recovery and few complications in repairing defects.

Objective To investigate the effects of zinc finger protein gene A20 on the inhibition of lipopolysaccharide (LPS) induced interleukin 8 (IL 8) expression in endothelial cells. Methods Plasmid pcDNA3.1EHA20 was transfected into human umbilical vein endothelial cells (HUVECs) by DOTAP method. The positive cell clones were screened with G418. The stable transfection and expression of A20 in HUVECs were determined by immunofluorescent analysis. IL 8 expression was detected by sandwich ELISA with double monoclonal antibodies. Results High expression of A20 gene in HUVECs transfected with pCDNA3.1EHA20 was confirmed by immunofluorescent analysis. IL 8 expression increased in LPS induced endothelial cells. A20 gene could inhibit more than 70% LPS induced IL 8 expression ( P

Medicine is a practical application subject,so medical students should conduct numerous clinical practice to improve operation skills and clinical diagnosis ability The ability of clinical practice is one of most important indicators to evaluate medical education quality.In recent years,medical students have been affected by many kinds of adverse factors,such as new doctor-patient relationship,the medical reform and employment pressure and so on.All these adverse factors will bring a great challenge to the dental clinical teaching.In the new medical situation we need urgently to solve the problems to guide students to improve clinical practice ability and cultivate qualified dental professionals

Objective Previous studies have demonstrated that LPS can induce endothelial cell activation and the expression of E-selectin. In this study, we examined whether A20 gene could inhibit the expression of E-selectin in endothelial cells induced by LPS. Methods With the help of DOTAP, endothelial cells were transfected with pCDNA3.1 EHA20. The postive cell clones were selected with G418.The stable transfection and expression of A20 in the endothelial cells were determined by immunofluorescence analysis. The E-selectin expression was checked by immunofluorescence, Western blot and in situ hybridization. Results Abundant A20 stable expression in endothelial cells transfected with pCDNA3.1 EHA20 was confirmed by immunofluorescence analysis. E-selectin expression increased in LPS-inducible endothelial cells. A20 gene inhibited 90% LPS-inducible E-selectin expression(P

OBJECTIVETo establish social stress induced depression-like model in mice of C57BL/6 strain, and to assess its reliability using differenf behavioral methods.

METHODSTotally 20 male mice of C57BL/6 strain were divided into the normal group and the stress model group by random digit table,10 in each group. Another 10 CD1 mice were subjected to social stress. Mice in the normal control group received no stress, while those in the model group received social stress for 10 successive days. Behavioral assessment was performed using social interaction test (SIT), the elevated plus-maze (EPM) test, tail suspension test (TST), respectively. Serum cortisol level was detected by ELISA to assess the reliability of the model.

RESULTSIn the social interaction test when the social target (CDI mice) was inexistent, mice in the normal control group spent longer time in the social interaction zone and less time in the corner zone (P < 0.05); mice in the model group spent less time in the social interaction zone and more time in the corner zone (P < 0.05). Compared with the normal group when CDI mice existed, mice in the model group spent less time in the social interaction zone and more time in the corner zone (P < 0.05). Compared with the normal control group, the total times for entry into open arms, close arms, and the maze were obviously reduced (P < 0.05), and the proportion of entering open arms was significantly reduced (P < 0.05) in the model group. In TST, the motionless time within the last 4 mm was prolonged in the model group (P < 0.05). The serum cortisol level in the model group was obviously elevated (P < 0.01).

CONCLUSIONSocial stress induced depression-like animal model in mice of C57BL/6 straineasquite reliable and possibly suitable to be used in integrative medicine research of combination of disease and syndrome model.

Animals ; Behavior, Animal ; Depression ; physiopathology ; Disease Models, Animal ; Hydrocortisone ; blood ; Male ; Mice ; Mice, Inbred C57BL ; Social Behavior ; Stress, Psychological

Humans ; Infant, Newborn ; Magnetic Resonance Imaging ; Magnetic Resonance Spectroscopy ; Male ; Sturge-Weber Syndrome ; diagnosis ; Tomography, X-Ray Computed

Objective To examine serum lipid levels and to investigate the risk factors of dyslipidemia in children and to study the indices of screening test for dyslipidemia of children in Beijing.Methods Serum total cholesterol(TC),triglyceride(TG),high density lipoprotein-cholesterol(HDL-c) and low density lipoprotein-cholesterol(LDL-c) were detected by HITACHI 7060 automatic biochemical analyzer in Beijing students.Body height, weight,upper body height,waist circumference,upper arm circumference and blood pressure were measured.Body mass index(BMI) was calculated according to the measurement of body height and weight.Waist-to-height ratio(WHR) was also calculated.Then a Logistic regression analysis and ROC study were conducted to examine the relationship between the above indices and dyslipidemia.Results Of 962 students,107 were found dyslipidemia.The prevalence rate was 11.12%.The upper body height,upper arm circumference,BMI,WHR,systolic pressure and diastolic pressure in children with dyslipidemia were higher than those of normal subjects(P