Objective: To study the marriage quality of different occupational groups. Methods: 200 married teachers from two universities and 144 married employees in hospitality industry in Hainan Province were applied Olson 's Questionnaire on Marriage Quality. Results: First, the total score of the teachers was higher than that of the employees (35. 1?6. 7/33. 5 ?6. 6, t =5. 59, P =0. 00) . Marriage satisfaction, character compatibility, communications between husbands and wives, while the arrangements of their incomes and leisure activities were also higher in teachers (t = 2. 20 -3. 37, P

Objective To study clinical characteristics of the thalamic hemorrhage,probe into the correlation factor of judged prognosis in early days and case-fatality rate.Methods To 2003—2005 96 thalamic hemorrhage sufferer's clin- ical data were analysed.Results The advanced age and high blood pressure is main cause of disease of the thalamic hemorrhage.Conclusion To observe the thalamic hemorrhage sufferer's vital signs have the certain reference value to judged prognosis,case-fatality rate and serious complications were related to bloods closely in early days,whether break into the brain room,hypothalamus and the brain stem whether implicate.

Objective:To investigate the value of YL-1 puncture hematoma evacuation for hypertensive intracerebral hematoma.Methods:138 patients with hypertensive intracerecbral hematoma treated by YL-1 puncture hematoma evacuative were studied retrospectively,and the relationship between the age,the time of operation,GCS and volume of hemorrhage with mortality were with logistic regression method analysis.Results:①There were no significant relation in all the ages;②the shorter the hemorrhage,the higher the mortality;③the lower GCS was,the higher the mortality was;④the bigger the hemorrhage volume,the higher the mortality.Conclusion:The present study indicates that age is not an factor to limit minimally invasive operation(YL-1 puncture hematoma evacuation).The effect of surgery depended on the time of hemorrhage,GCS and volume of hemorrhage.

Objective:To evaluate and analyze the application value of Acute Physiology and Chronic Health EvaluationⅢ(A- PACHEⅢ)in evaluation of the severity and prognosis of patients in SCIU.Methods:By collecting and sorting out the data of 341 cases of surgical serious patients,the severity and prognosis of patients were evaluated by APACHEⅢ.Results:Making comparison among the different types of diseases,the scores and mortality of cerebral hemorrhage and multiple hurt were high- er than those of the diseases(P

Objective To understand the clinical medicine graduates competency status of Kunming Medical University in the past 5 years and provide important reference for cultivating students in medical university. Methods The questionnaire results in the relevant departments responsible person of Yunnan provincial municipal and district 26 hospitals were system analyzed by fuzzy comprehensive evaluation method. Results In the past 5 years, the graduates occupation competence of clinical medicine in Kunming Medical University is good. Among them, the graduates’core competency gets the lowest score, but the graduates’post competency gets the highest score. Conclusion The graduates’core competency should be strengthened in training of doctor-patient communication skills and quality evaluation of clinical practice for medical students.

OBJECTIVE:To establish the method for simultaneous determination of 5 heavy metals in Vespae Nidus,and to dis-cuss the effects of difterent producing area and part on the contents of heavy metals in Vespae Nidus. METHODS: The contents of arsenic(As),mercury(Hg),lead(Pb),cadmium(Cd)and copper(Cu)were determined by atomic spectrophotometry(hydride generation method,cold vapor method,graphite furnace method and flame method). The difference in the contents of heavy metals in samples were compared among different producing areas by analyzing data. RESULTS:The linear range of As,Hg,Pb,Cu, Cd were 0-40 ng/mL(r=0.9930),0-0.9 ng/mL(r=0.9974),0-80 ng/mL(r=0.9955),0-8 ng/mL(r=0.9927),0-800 ng/mL(r=0.9966),respectively. Limit of quantitation were 4.473,2.35×10 - 3 ,8.380,2.128,54 ng/mL,limit of detection were 1.342,0.701× 10 - 3,2.514,0.632,16 ng/mL. RSDs of precision,stability and reproducibility tests were all lower than 5.0%. The recoveries were 84.26% -102.25%(RSD=7.71% ,n=6),90.90% -115.31%(RSD=9.38% ,n=6),92.16% -105.70%(RSD=6.19% ,n=6), 90.81%-113.99%(RSD=8.86%,n=6),91.63%-102.79%(RSD=5.09%,n=6). The phenomenon that heavy metals of sample from Shandong and Hebei exceed the standard was the most serious. Main heavy metal which exceed the standard was Pb. The con-tent of Pb in Chamber was higher than other parts. CONCLUSIONS: The method is simple,precise,stable and reproducible,and can be used for simultaneous determination of 5 heavy metals in Vespae Nidus. There are different contents of heavy metal in Ves-pae Nidus of different producing areas and parts.

Objective To compare the changes of corneal endothelial cells in early recovery period after 1.8 mm small incision phacoemulsification nucleus chopping and conventional incision phacoemulsification for cataract.Methods Fifty-three patients with cataract were divided into the observation group and control group according to different operation methods.The observation group was treated with 1.8 mm small incision phacoemuisification nucleus chopping while the control group was treated with routine incision phacoemuisification.The visual acuity,degree of astigmatism,corneal endothelial cell indexes and complications were observed.Results The effective ultrasound time and average ultrasonic energy of the observation group were significantly less than those of the control group (all P ＜ 0.05).Compared with 1 day before operation,the visual acuity,degree of astigmatism,corneal endothelial cell area (ECA) and coefficient of variation (CV) were significantly increased at 1 week and 4 weeks after operation,and corneal endothelial cell density (ECD) was significantly decreased (all P ＜ 0.05).The visual acuity and ECD levels of the observation group at 1 day,1 week and 4 weeks after operation were significantly higher than those of the control group,while the degree of astigmatism,ECA and CV were significantly lower than those of the control group (all P ＜ 0.05).Conclusion Compared with the traditional incision phacoemulsification,the damage of 1.8 mm small incision phacoemulsification nucleus chopping to corneal endothelial cells is significantly milder,can reduce the effective ultrasound time and average ultrasonic energy,decrease the degree of astigmatism,improve the visual acuity and decrease the corneal edema.

Objective:To evaluate the analgesic effect of botulinum toxin type A (BTX-A) in osteoarthritis model and the changes of Nav1.8 protein expression in spinal ganglia changes.Method:Animal model of knee osteoarthritis (KOA) was established by intra-articular injection of 4％ papain solution 0.3ml into SD rat right knee.After the formation of arthritis,they were randomly divided into two groups at the 2nd day:BTX-A group (n=10):intra-articular injection of 5μl BTX-A 0.1IU;WFI group (n=10):intra-articular injection of 5μl water.No papain or BXT-A was given to the sham group (n=10).At the 1st,3rd,5th day after injection,we tested the pain behavior,thermal pain threshold,and sodium channel 1.8 (Nav1.8) protein expression in spinal ganglion by using immunohistochemistry.Result:Analysis of spontaneous pain behavior showed abnormal gait caused by rat osteoarthritis.Comparing with the WFI group,abnormal gait caused by osteoarthritis improved significantly in BTX-A group.At day 5 it improved more significantly than day 1 and day 3.Thermal pain threshold of BTX-A group increased more than that of WFI group (P＜0.05) at any time point.Abnormal high Nav1.8 protein in model rats decreased in spinal ganglia for BTX-A group.Conclusion:The intra-articular injection of BTX-A may play the analgesic effect in the model of KOA by down-regulating of the expression of Nav1.8 protein in spinal ganglia and reduction in the central sensitization to pain stimulation.

Objective To study the effect of new generation histone deacetylase inhibitor LBH589 single-drug or combined with the mouse serum which contains the radix echinopsis on multiple myeloma (MM) cell line U266 and their mechanism.Methods The acetylation level of α-tublin which was a substrate of HDAC6 was detected by Western blot.The chemical force between Heat shock protein 90 (HSP90) and its client proteins was detected by immune precipitation (IP).Results The different concentrations of LBH589 single drug (0,20,50 nmol/L),and 50 nmol/L combined with the mouse serum which contained the radix echinopsis (1 g/ml) were able to inhibit the proliferation of U266 cell.With the increase of drug concentration and the extension of time,the acetylation levels of α-tublin and HSP90 increased gradually (at 24 or 48 hours) in a dose dependent (P ＜ 0.05).The inhibition of LBH589 combined with the mouse serum was stronger than that of LBH589 single drug (P ＜ 0.05).Conclusion LBH589 could inhibit the growth of MM cells and their cell cycles,and induce the apoptosis of MM cell line U266.

As a safe and noninvasive therapy, repetitive transcranial magnetic stimulation (rTMS) and theta burst stimulation (TBS) are used to change the cortical excitability, and induce or enhance neural plasticity, which can promote the recovery of motor function after stroke. This paper summarized the individual treatment of rTMS on motor function of stroke patients, the advantages of TBS compared with rTMS, and the mechanisms and clinical application of TBS.