ObjectiveTo explore the clinical value on the detection of urinary podocyte marker protein podocalyxin(PCX) in children with nephritic syndrome(NS) and Schonlein-Henoch purpura nephritis(HSPN).MethodsUrinary samples voided in the morning were obtained from 14 healthy children and 75 children with NS or HSPN or Schonlein-Henoch purpura(HSP),including 21 children with NS in the acute phrase,14 children with NS in the catabasis,16 children with HSPN in the acute phrase,14 children with HSPN in the catabasis,10 children with common HSP,and 14 healthy children for control group.And urinary PCX content of the first morning urine was quantified by enzyme-linked immunosorbent assay(ELISA).SPSS 13.0 software was used to analyze the data.Results1.The levels of PCX content were significantly higher in the urine from children with any case of NS and HSPN compared with those in the control group(P≤0.009),but there was no obvious difference between common HSP children and children in the control group(P=0.754).2.The level of urinary PCX content in acute phrase of NS was(0.593?0.271) ?g/L,in the catabasis of NS was(0.162?0.093) ?g/L,there were significant difference(P=0).The level of urinary PCX content in acute phrase of HSPN was(1.822?1.342) ?g/L,in the catabasis of HSPN it was(0.236?0.141) ?g/L,which was significantly different(P=0.004).The level of urinary PCX content in common HSP was(0.089?0.061) ?g/L,there were significant difference in any case of HSPN(Pa

Objective To investigate and improve the methods of nasal feeding to reduce the occurrence of complication such as diarrhea and vomiting.Methods We separated 140 patients with heavy craniocerebral injury into the control group (50 patients) and the experimental group (90 patients) according to different nasal feeding methods. For the control group traditional method was used. For the experimental group infusion pump was used to control infusion rate. The incidence of complication was compared between two groups. And within experimental group, the same nutrient solutions in respect of type and volume were infused during 10 hours, 16 hours, and 18 hours respectively. The relationship between infusion rate and the occurrence of complication was observed, analyzed and compared to seek for the most suitable enteral nutrition route which incurred the least complication.Results The incidence of diarrhea and vomiting in the experimental group was lower than that of the control group. And there were no significant differences in the occurrence of complication by using infusion pump at three different infusion rates within the experimental group. Conclusions The application of infusion pump in nasal feeding can reduce the incidence of complication and infusing nutrient solution at a slow rate was not necessary.

Child, Preschool ; Dental Caries ; prevention & control ; Humans ; Infant

Objective: To observed the efects of estrogen and wall-shear stress alone or in combination on the proliferation and function of rat osteoblast in vitro. Methods: Isolated and purified osteoblast from the calvaria of newborn SD rats were cultured and passaged.The cells of passes three to four were treated with 0.1 nmol/L estrogen (goup E),wall-shear stress at 80 r/min (group WSS) or 0.1 nmol/L combined with wall-sher stress at 80 r/min (group EWSS) respectively.Cell proliferation was studied by MTT assay and alkline phosphatase (ALP ) by a ALP test kit. Results: Both the estrogen and wall-shear stress alone caused the increase of cells proliferation and alkaline phosphatase (ALP) activity.But long period of wall-shear stress decreased the cell proliferation.Wall-shear stress increased ALP activity more quickly and more remarkably than the estrogen did.The combination of estrogen and wall-shear stress increased the cell proliferation and the ALP activity.In the early stage (6～24 h) of the cell proliferation, the combination functioned synergicly.The combination functioned antagonistically on ALP from 6 to 12 h while synergicly after 12 h. Conclusion: Estrogen and wall-shear stress can elevate the cell proliferation, ALP activity of osteoblasts in vitro.

Acupuncture Therapy ; Female ; Humans ; Polycystic Ovary Syndrome ; therapy

OBJECTIVETo observe the effect of catgut implantation at acupoint on the content of tumor necrosis factor alpha (TNF-alpha), and to explore its mechanism for treatment of ankylosing spondylitis (AS).

METHODSSeventy-four AS patients were randomly assigned to the observation group and the control group by digit random table, 37 in each group. Patients in the control group took Sulfasalazine Enteric-coated Tablet (SECT), 0.5 g each time, three times daily for 3 months. Those in the observation group additionally received catgut implantation at acupoint for 3 months. Changes of visual analogue scale (VAS), Schober test, pillow wall distance, thoracic activity, erythrocyte sedimentation rate (ESR), C reactive protein (CRP), and TNF-alpha were compared after 3 months of treatment.

RESULTSThere was obvious change in VAS, Schober test, pillow wall distance, thoracic activity, ESR, CRP, and TNF-alpha after treatment in the both groups, showing statistical difference when compared with before treatment (P < 0.05, P < 0.01). The aforesaid indices were obviously improved after treatment in the observation group (P < 0.05, P < 0.01).

CONCLUSIONCatgut implantation at acupoint, as an assistant therapy, could significantly improve clinical symptoms and signs, decrease the level of TNF-alpha, and its effect was superior to that of oral SECT administration alone.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Spondylitis, Ankylosing ; blood ; therapy ; Tumor Necrosis Factor-alpha ; blood ; Young Adult

ObjectiveTo investigate the characteristic of aphasia after left Basal ganglia injured.Methods10 cases with focus at left Basal ganglia and aphasia were assessed with Chinese Rehabilitation Research Center's Chinese Standard Aphasia Examination 12 weeks after stroke.Results and ConclusionThe characteristics of these 10 patients did not only show similar to the characteristics of Basal ganglia aphasia,but some other features.The relationship of the symptom and imaging finding has been discussed.

Since the symptoms and mechanism of poststroke aphasia are various and complicated, there are a great deal of problems which are difficult to resolve yet. At the aspect of aphasia research, Chinese and Western medicine have different emphases, Chinese medicine emphasizes particularly on therapy, Western medicine emphasizes on discussing mechanism of aphasia, all kinds of sorts and evaluation of aphasia and pertinence rehabilitation, and so on; modern research methods include neurolinguistics, neuropsychology, cognitive science, neurorehabilitation, neuroelectrophysiology, neuroimage and so on, all these means of aphasia research often involve in cross multi-subject, furthermore becoming powerful implement of recognizing brain mechanism.

Noun and verb are always two primary lexical knowledge information in all kinds of languages. It plays an important role in the structure and function of the language. Cognitive activation and differential neural basis when aphasiacs distinguish from these two kinds of lexical knowledge information is the focus of subjects, such as neuropsychology, neurolinguistics and neuroimage. The specific deficit regarding the precessing of noun and verb was analyzed in the article with the data of this aphasiac.

Brucellosis ; diagnosis ; drug therapy ; Child ; Child, Preschool ; Female ; Humans ; Male