Objective:To explore the expression of MIP-1? during healing of skin incised wound and the applicability of time-dependent expressions of MIP-1? to determination of wound age. Methods:An incised wound was made in the dorsal skin of mouse. Immunohistochemical and image-analysis techniques were employed in vital skin wounds 1h~14d after incision and postmortem incision 0.5~6h after death, and the non-incised mouse skin was used as contro1.Results:In the vital skin incisions,expression of MIP-1? began at 6h after incision,which increased subsequently,and peaked at 3d.The quantity of MIP-1?expression decreased and minimized in the specimens aged 14 days. In the non-wounded groups and postmortem incision groups MIP-1? was only detected as weak expression in the epidermic cells,sweat gland cells and endothelial cells. Conclusion:The time-dependent expression of MIP-1?during healing of skin incised wound may be used as a useful reference marker.

Cardiology ; Child ; China ; Congresses as Topic ; Humans ; Japan ; Korea

Finite element is a common means to study pelvic biomechanics. This article introduces current methods to establish finite element model and application advances of the models in pelvis,and explores issues and future developing direction of pelvic finite element models.

Child ; Humans ; Practice Guidelines as Topic ; Syncope ; diagnosis

Objective The purpose of this study was to investigate the relationship of p185 and p53 protein overexpression with pathological types and recurrence.Antibodies against p185 and p53 proteins were used to measure expression of these proteins in the nuclei or cell membrane of cells from sections of the giant cell tumor of bone GCT.It showed that 11 out of 52 tumors overexpressed p185 protein and 14 out of 52 tumors had abnormally high levels of p53 protein,4 cases had abnormally high levels of both p185 and p53 proteins,positive expression rates of p185 and p53 in cases with recurrence and cases without reccarrence were 46 2%,20 5% and 38 5%,15 4% respectively.However,there was no association between p185 and p53-positive cases and pathological degree.There was significant correlation between the expression of p185 and p53 protein in the giant cell tumor of bone and recurrence.(? 2=6 125,P=0 047).However,there was no statitically significance between the expression of p185 and p53 protein in the giant cell tumor of bone and pathological types.So that,we consider that the clinical significance for p185,p53 overexpression in GCT to be researched further.

Objective To observe the effect of enhanced extracorporeal counterpulsation (EECP) on intraocular pressure (IOP). Methods 25 patients were measured their IOP bilaterally with Schoitz tonometer before and after EECP. Results The IOP decreased in both left and right eyes after EECP (P<0.01). Conclusion EECP can reduce intraocular pressure.

0.05),while that of serum P was significant(P

Objective To observe the effect of synthetic corticotropin with vitamin B 6 on intractable epilepsy of children.Methods 20 patients (3 months to 4 years old) among which 9 children with infantile spasms and 11 children with intractable epilepsy were injected with vitamin B 6 (50～100 mg/d) for 10 days,then treated with cortrosyn 0 015~0 025 mg/Kg?d by the interval mothod of lengthening dosage,total course of treatment was about 2 months,seizure,EEG and some blood biochemical tests were observed in all patients before and after the course of treatment.Results The seizure of 14 patient children stopped after the treatment,seizure frequency was decreased in 5 patients (from 15 to 40 times/week decreasing to 3 to 14 times/week respectively),1 patient with intractable epilepsy was stopped treating because of side effect.The general clinical condition and EEG were also improved.The blood biochemical tests about liver and kidney were normal during treatment,hypokaliemia,hypocalcemia and slight dropsy were appeared in some patients.Conclusion Cortrosyn with vitamin B 6 is effective for infantile spasms and intractable epilepsy in children of low age.

Objective To investigate the efficacy and safety of Topiramate as adjunctive therapy in patients with refractory epilepsy.Methods 115 patients enrolled in this open-label,self-controlled prospective study which were designed to adopt slower dosage escalation schedule with lower initial,until reached target dosages(up to 8 weeks) or the most tolerable dosages of Topiramate,and maintained them 1~3 years treatment.The long-term efficacy,safety of Topiramate and their relationship with dosage were evaluated.Results The median percentages reduction were 56.0%,75.8%,76.1%,77.3% and 78.1% after 8 weeks,6 months,1 year,2 years and 3 years Topiramate treatment respectively for adults,and 32.1%,66.7%,68.9%,70.1% and 70.8% respectively for children.The overall efficacy for partial seizures(70.6%) was higher than that for generalized seizures(37.5%)((P

Objective To summarize and analyze the experience of nursing patients with congenital heart disease and pulmonary hypertension undergoing intervention therapy.Method The nursing measures included psychological nursing, preoperative preparation,postoperative monitoring of pulmonary hypertension and vital signs, observation and prevention of complications,and transfusion management.Result All the 18 patients recovered well after intervention therapy without severe complications.Conclusion The peri-intervention nursing of patients with congenital heart disease and pulmonary hypertension is important for increasing interventional success rate and lowering postoperative complication.