Objective: To explore the pharmaceutical care mode for drug treatment and interactions through participating in the treatment of patients using cyclosporine A combined with anti-tuberculosis ( TB) drugs. Methods: The clinical questions were pro-posed and refined, the literature evidence was retrieved in the electronic databases, the questions were answered and the procedure and mode of pharmaceutical care for the similar clinical problems was established. Results:For the patients with cyclosporine A treatment, strong anti-TB drugs should be chosen to ensure the effectiveness and decrease the drug interactions, and the concentration monitoring of cyclosporine A should be paid more attention. Conclusion: Clinical pharmacists can implement the pharmaceutical care for the pa-tients with immunosuppression and TB infection through developing anti-TB regimen, avoiding the drug interactions, improving the drug safety and so on.

Objective To study the optimum culture media and liquid conditions in shaking flasks for laccase production by Ganoderma lucidum. Methods Taking S_3 stain of G. lucidum as test materials and laccase activity of G. lucidum as measurement index to optimize the culture media and liquid conditions through orthogonal test. Results The optimum culture components of media were: Glucose 30 g/L, cotton 0.2%, (NH_4)_2HPO_4 0.66 g/L, casein 0.5%, Tween-80 0.15 mL; the optimum conditions were: initial pH value of medium was 5.5, 75 mL medium was in 250 mL-flask, inoculation was 12.5%, mycelium age was 5?24 h for 9?24 h culture. Conclusion Laccase activity of G. lucidum has been improved remarkably in the optimum culture media and liquid conditions.

Objective To study the effect of clopidogrel on serum inflammatory factors and echocardiography in patients with acute myocardial infarction complicated with heart failure.Methods60 cases of patients admitted in our hospital from July 2014 to August 2016 were selected as the research object in this study.They were randomly divided into the control group and the experimental group, each group had a total of 30 cases.Patients in the control group were treated with diuretics, nitroglycerin and aspirin, while the experimental group received clopidogrel on the basis of the control group.After treatment in June, comparative analysis of the experimental group and the control group of patients with treatment effect and related clinical indicators.ResultsAfter treatment, 6months after the corresponding, compared with before treatment, the experimental group and the control group of patients with C-reactive protein and vascular cell adhesion molecule 1, 6min mediated leukocyte walking experiment, Su-6 and the heart rate of treatment were improved, with statistical difference (P<0.05), left ventricular end diastolic there was no significant difference between the change in diameter.In the control group, C-reactive protein, vascular cell adhesion molecule 1, 6min walking test, interleukin-6 and heart rate were significantly better than those in the control group, with statistical difference (P<0.05).ConclusionThe application of clopidogrel in acute myocardial infarction complicated with heart failure in the can to a great extent on the inflammatory response in patients, improve the patient's left ventricular ejection fraction and heart rate, with the further promotion and application in clinical significance.

Objective: To investigate the expression of nuclear factor-κB (NF-κB) subunits P50 and c-Rel protein in primary cortical neurons of Wistar rats at different time points of oxygen glucose deprivation/ reoxygenation(OGD/R). Methods: The neurons dissociated from the cortex of the neonatal rats were primary cultured and were identified by immunocytochemistry. OGD/R model was established. The study was divided into 6 groups according to different processing methods,including normal group,OGD 4 h treated,OGD 4 h/R 2 h treated,OGD 4 h/R 6 h treated,OGD 4 h/R 12 h treated and OGD 4 h/R 24 h treated groups. The expression of NF-κB P50 and c-Rel protein in neurons was examined by immunocytochemistry method and Western blotting. Results: (1) Immunocytochemistry detection targeting neuron specific enolase (NSE) and beta-III tubulin confirmed that the cultured cells were neurons. (2) The expression of NF-κB P50 protein was significantly higher in OGD 4 h group than in control group(P<0.05); the expression continued to increase in OGD 4 h/R 2 h andOGD 4 h/R 6 h groups, and reached its peak 6 h after reoxygenation (P<0.01),then began to decrease,but the expression in OGD 4 h/R 12 h group was significantly different from that of the control group (P<0.05); there was no significant difference between OGD 4 h/R 24 h group and control group. (3) The expression of NF-κB c-Rel protein was similar between OGD 4 h group and the control group; the expression increased in OGD 4 h/R 2 h and OGD 4 h/R 12 h groups and reached its peak 12 h after reoxygenation(P<0.01),and did not recover to the normal level in OGD 4 h/ R 24 h group (P<0.05). Conclusion: Oxygen glucose deprivation/reoxygenation can activate NF-κB subunits P50 and c-Rel in the primary cortical neurons in rats in a time-associated manner.

Objective:To understand the epidemic situation of drinking-water-borne endemic fluorosis in Fujian Province, and to provide more information for control and evaluation of the disease.Methods:In 2019, all villages in the 36 drinking-water-borne endemic fluorosis areas in Fujian Province were selected, in which the situation of water improvement was investigated, fluorine content of drinking water was tested and dental fluorosis of all the children aged 8 to 12 was examined. The urinary fluoride content and skeletal fluorosis of people over 25-year old was investigated in some of those villages.Results:A total of 153 disease affected villages were investigated, in which the water improvement projects were all completed. The fluoride content in each tap water sample of the water improvement project ranged from 0.00 to 1.05 mg/L, and the qualified rate was 100.00% (153/153). The normal operation rate of the water improvement projects was 95.42% (146/153). The detection rate of dental fluorosis in children aged 8 to 12 was 2.72% (76/2 789) with a dental fluorosis index 0.07. The detection rate of children's dental fluorosis was statistically different in different age groups, so was it in areas with different coverage rate of water improvement projects ( P < 0.05). The geometric mean of urinary fluorine level in adults was 0.80 mg/L ( n = 3 765), and the detection rate of skeletal fluorosis was 2.00% (6/300). Conclusion:Although some achievements have been made in the prevention and control of drinking-water-borne endemic fluorosis in Fujian Province, it is still necessary to further consolidate and enhance the water improvement projects.

Adult ; Aged ; Bronchoalveolar Lavage ; methods ; Critical Pathways ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; therapy

Aortic Coarctation ; complications ; Ductus Arteriosus, Patent ; complications ; Humans ; Infant ; Pulmonary Artery ; abnormalities

ObjectiveTo comprehensively evaluate the curative effect ofTCM reating type 2 diabetes with three type dialectical therapy by Fuzzy Mathematics.Methods261 cases of 2 type diabetes were divided into three groups by syndrome differentiation,as follows:type of heat with yin deficiency,type of deficiency of both qi and yin,and type of deficiency of both yin and yang,and received corresponding therapies.The course of treatment was 24 weeks,in the first 12 weeks (period 1),on the basis of western medicine treatment,patients of the three groups were respectively treated by qingrungranules,ziyigranule and shuangtiaogranules.In the next 12 weeks (period 2),all patients were only treated by western medicine.The value of FBG,P2BG,HbAlc,TC,TG,BMI of the two periods were comprehensively evaluated by the methods of fuzzy mathematics.ResultsAt the end of period 1,the total effective rate of the type of heat with yin deficiency was 91.38％,the type of deficiency of both qi and yin was 87.16％,and the type of deficiency of both yin and yang was 83.34％; while at the end of period 2,the total effective rate of the three types was 59.18％,48.65％a nd32.65％ respectively.ConclusionThe effective rate was obviously improved in period 1; while in period 2,the effective rate was decreased.

Objective To evaluate the influence of pregnancy outcomes with different dosage of estradiol valerate in addition to progesterone for luteal supplementation in IVF-ET cycles. Methods From February 2011 to October 2012, 362 patients undergoing IVF-ET in the second affiliated hospital of Kunming Medical University were selected. The patients were randomly assigned into three groups. Since oocyte retrieval, all of them were injected luteal supplementation with progesterone in oil 60 mg/d, im. Group A as control group (112 cases) did not add with pentanoic acid estradiol;Group B for the experimental group (122 cases), added pentanoic acid estradiol 4 mg/d after embryo transfer;Group C for the experimental group (128 cases) added pentanoic acid estradiol 6 mg/d after embryo transfer. Finally the pregnancy outcome and implantation rate were compared for each group. Results There were no significant differences between group A and group B, group B and group C in the rate of embryos grow and pregnancy rate ( >0.05) . The embryo planting rate and pregnancy rate have significant difference between Group A and group C ( < 0.05) . Conclusion 6 mg/d fill estradiol valerate for luteal supplementation may improve implantation rate and pregnancy rate after IVF.