Objective To determine the effect of exogenous wild-type p53(WT-p53) on the growth of hepatocellular carcinoma (HCC) cell lines possessing different p53 functional status.Methods With recombinant adenoviral vector expressing WT-p53(Ad-p53),p53 gene was transfected into HCC cell lines:Bel-7402,QGY-7701, and PLC/PRF/5.The cytotoxicities of Ad-p53 to these cell lines were measured by MTT assay,cell growth properties and cell cycle pattern were assessed by flow cytometry.Results The OD of PLC/PRF/5, Bel-7402,and QGY-7701 cells transfected with Ad-p53 of 40,200,and 800 MOI were 0.02?0.00 vs 0.98?0.16(P0.05),compared with control groups respectively. Analysis of flow cytometry showed higher G2/M? lower S and apoptosis ratio in PLC/PRF/5 cells,while higher G0/G1 ratio and lower S ratio in Bel-7402 cells. ConclusionReplication-deficient adenoviral vector expressing WT-p53 may be useful for gene therapy in HCC regardless the status of endogenous p53.

Objective To establish a recombinant eukaryotic expression vector containing human interferon-?(IFN-?) cDNA,and investigate the expression of IFN-? gene in transfected hepatocellular carcinoma (HCC) cell lines. Methods pcDNA3-IFN-?,established by subcloning IFN-? cDNA into a mammalian expression vector pcDNA3,was mediated into HCC cell lines SMMC-7721 and QGY-7701 by lipofectamine. The transfected HCC cells were selected in RPMI1640 containing G418(400～700?g).RT-PCR analysis and ELISA assay were used for mRNA transcription and protein expression of IFN-? gene. Results IFN-? mRNA was detected only in pcDNA3-IFN-? transfected cell lines, and high levels of IFN-? protein were detected only in the supernatants of IFN-? gene-modified cell lines. Conclusions pcDNA3-IFN-? can stably expressIFN-? cDNA in the transfected HCC cell strains

OBJECTIVE:To select a technique for preparation of fluconazole for injection and to establish a method for determination of its content.METHODS:The formula was selected on the basis of pH value and species of solution adjuvant.The content was determined by HPLC.RESULTS:The preparation was stable when pH value was between6.5～8.5,and its clarity could be increased by propylene glycol.The detectable concentration of fluconazole showed a good linear correlation in the range of40～200?g/ml.The average recovery was100.37%,RSD=1.37%(n=3).CONCLUSION:The fluconazole for injection prepared by the present technique is stable in quality and the method for content determination is accurate and practicable.

Child ; Cystic Fibrosis ; diagnosis ; diagnostic imaging ; genetics ; Female ; Genetic Testing ; Humans ; Radiography ; Sweating

(P < 0.05 ).Scanning electron microscopy showed that the three-dimensional structure of mix-biofilm in control group was more complex.As real-time PCR showed,after 6-hour culture,the expressions of icaA,fbe,aap,hwp1, als3 and efg1 genes in Farnesol group were down-regulated compared to those in control group and after 24-hour culture the expressions of aap,hwp1,als3 and efg1 genes in Farnesol group were down-regulated compared to those in control group.Conclusion With the intervention by fungal quorum sensing molecules Farnesol,the structure in control group became denser and more complex than that in Farnesol group.This change may have a closer correlation with the down-regulated expressions of als3,hwp1 and efg1 genes,which are related to the formation of Candida albicans biofilms.

OBJECTIVE To analyze the etiological distribution and drug resistance characteristics of patients with respiratory failure and lung infection in EICU.METHODS The types of respiratory failure,the incidence condition of ventilator-associated pneumonia(VAP),the mortality rate,and the drug sensitivity of 129 pathogen strains which were isolated and cultured from the 110 cases with respiratory failure and pneumonia between Jan 2006 to Aug 2008 in EICU were analyzed.RESULTS Among the 110 patients,48 cases were died(the mortality rate of 43.6%).There were 28 cases(58.3%) of Ⅱ type respiratory failure,20 cases(41.7%) of Ⅰ type respiratory failure.Of 129 pathogens strains,there were 29 Gram-positive bacteria(22.5%) and 100 Gram-negative bacteria(77.5%).The major Gram-negative bacteria were Pseudomonas aeruginosa,Acinetobacter baumannii,and Klebsiella pneumoniae,and they showed generally higher multidrug resistance.Except minocycline,A.baumannii appeared strong multidrug resistance to the other 16 kinds of common antibiotics.The main Gram-positive bacteria were Staphyococcus haemolyticus,S.aureus and coagulase-negative staphylococci(CNS),and no evidence showed they resisted to vancomycin.CONCLUSIONS There are high mortality rate of patients with respiratory failure and lung infection if they suffered from compound infection.The main pathogens of the lung infection are Gram-negative bactiera,such as P.aeruginosa,A.baumanniiand K.pneumoniae in order,with high multidrug resistance.The main Gram-positive bacteria show no evidence resist to vancomycin.

Objectives To explore the clinical symptoms, therapy and prognosis of tachycardia-induced cardiomyopathy (TIC) in children. Methods Clinical data of 22 children with TIC from July 2007 to July 2012 were retrospectively analyzed. Results TIC was mostly seen in male infants and 81.82%of TIC was caused by atrial arrhythmias. The clinical symptom relieved after arrhythmia and ventricular rates were under control with average effective treatment time of (14.00 ± 8.20) days. Ten patients had tachycardia recurrence, 7 of them had atrial arrhythmia and their clinical symptoms were improved after treatment;while 3 of them showed longer time of therapy with average treatment time of (19.50±8.40) days (P<0.05). Five children underwent radiofrequency ablation before school age and got good therapeutic effect. The post-treatment echocardiographic parameters showed cardiac function of TIC children was significantly improved after treatment, including left ventricular end-diastolic diameter index, left ventricular end-systolic diameter index, left ventricular ejection fraction and shortening score (all P<0.05). Conclusions Childhood TIC is a reversible myocardial dysfunction and its prognosis is good. TIC can be induced by various types of tachyarrhythmias and normally by atrial arrhythmia. The preferred treatment of TIC is administration of antiarrhythmic drugs but radiofrequency ablation is needed to ventricular arrhythmias induced TIC.

Objective To evaluate clinical outcome and complications of mesh-augmented vaginal reconstructive surgery in treatment of pelvic organ prolapse. Methods From Feb 2007 to Jan 2009, meshaugmented vaginal reconstructive surgery were performed on 66 women with pelvic organ prolapse stage Ⅲ-Ⅳ. Pre and postoperative symptoms, pelvic organ prolapse quantitation (POP-Q) stage and pelvic floor distress inventory-short form 20 (PFDI-20) measurements were studied to assess anatomic and quality-of-life outcome. Operative complications were also analyzed. Results Totally 65 patients underwent successful surgeries. The rate of follow-up was 97% (63/65) with a median follow-up of 17. 2 months. Subjective cure rate and objective cure rate were both 97% (61/63) at 6 and 12 months after surgeries, 51 women completed PFDI-20 measurements and scores were 102 ± 50 before surgery, 16 ± 21 at 6 months and 15 ± 20 at 12 months. It reached statistical difference when scores were compared before and after surgeries ( P ＜0. 05). Among 66 patients, 2 patients underwent organ injuries, 2 had recurrent prolapse, 4 had meshrelated complications and 1 had severe de novo stress urinary incontinence. Six patients underwent second surgery. Conclusions Mesh-augmented vaginal reconstructive surgery in treatment of pelvic organ prolapsed brought satisfied clinical outcome. The incidence of mesh-related complications was low and secondary operative interventions were effective.

Objective To investigate the correlation between specific features on contrast-enhanced CT and its clinical findings of adrenal tuberculosis so as to improve the diagnostic accuracy of the disease. Methods Contrast-enhanced CT features in 30 patients with documented adrenal tuberculosis were retrospectively evaluated blindly for the features of location,size,morphology,attenuation and enhancement patterns on CT images,and compared with clinical and pathological materials.Results The common CT manifestations were as follows:enlargement of the adrenal glands in all 30 cases(bilateral 90%,mfilateral 10%)including mass-like enlargement in 13 cases and enlargement but the contours preserved in 17 cases, heterogeneity(28 cases,93.3%),calcification(17 cases,56.7%),and low attenuation in the center with peripheral enhancement(16 cases,53.3%)of the lesions.After antituberculosis chemotherapy, 5 cases of enlarged adrenal glands decreased in size or returned to normal size and configuration,with disappearance of the central low attenuation and new appearance of dot-like calcification in 2 cases.Cochran Armitage trend test showed there was an increasing tendency of calcification rate with clinical duration(X~2= 7.47,P

This study aimed to investigate the relationship between clinical features of myelodysplastic/myeloproliferative disease, unclassifiable (MDS/MPD-U), karyotype of chromosome and JAK2 mutation in 1 case. The clinical features, karyotype and JAK2 mutation of the patient with MDS/MPD-U were studied by means of bone marrow biopsy, karyotype analysis and ARMS-PCR technique. The results indicated that the typical micromegakaryocytes and thrombocytosis, karyotype aberration of trisomy 8 as well as JAK2 V617F mutation were found in this patient. It is concluded that the patient was diagnosed as MDS/MPD-U with trisomy 8 and JAK2 V617F mutation. The data of this patient will provide evidence for studying correlation of chromosome karyotype aberration with JAK2 V617F mutation and for evaluating prognosis of MDS/MPD-U.
Chromosomes, Human, Pair 8 ; Female ; Humans ; Janus Kinase 2 ; genetics ; Karyotyping ; Middle Aged ; Mutation ; Myelodysplastic-Myeloproliferative Diseases ; classification ; genetics ; Trisomy