The effects of sophoramine (SA) on myocardial infarction size and ischemia－induced and reperfusion－induced arrhythmia were studied in conscious rats. It was found that pretreatment with SA 13.3 and 15 mg/kg iv reduced significantly infarct size and cardiac LDH release at 6h afte left descending coronary artery ligation as compared with the control. SA 10.6, 13.3 and 15mg/kg iv decreased markedly the early ischemic arrhythmia in conscious rats. Amiodarone 7.5mg?kg－1 had the same effects on the prevention of ischemic arrhythmia. In reperfusion experiment,it decreased the incidence of ventricalar fibrillation and significantly shortened the duration of arrhythmias. These results suggest that SA has protective effects on myocardial ischemia and reperfusion injury.

Objective:To investigate the nursing experience of post cesarean section for a patient with nutcracker syndrome and pregnancy-induced hypertension.Methods:The clinical data of a patient with nutcracker syndrome and pregnancy-induced hypertension in our hospital in March 2020 was analyzed retrospectively. Multiple care measures including psychological care, posture and activity, pipeline care, diet and breastfeeding care were given after cesarean section to prevent the occurrence of complications.Results:After 5 days of nursing care such as disease observation, posture and activity, blood pressure control, the condition of this patient was improved and then discharged.Conclusions:Implementation of refined nursing measures including posture and activity, pipeline care, diet and breastfeeding care to pregnant women with nutcracker syndrome, especially those with hypertension, can reduce the incidence of serious complications and play an important role in the recovery of patient.

Astragalus Plant ; Cell Differentiation ; drug effects ; Cell Division ; drug effects ; Codonopsis ; Drugs, Chinese Herbal ; pharmacology ; Hematopoietic Stem Cells ; cytology ; drug effects

Objective To observe the effect of new moist dressings on pressure ulcers after spinal cord injury. Methods 58 spinal cord injured patients complicated with pressure ulcers were divided into observation group (n=29) and control group (n=29). The observation group accepted moist dressings, while the control group accepted routine dry dressings, TDP and ultraviolet irradiation. The incidence of im provement were recorded 2 weeks after treatment, and the time of healing were compared. Results 11 cases cured, 17 cases relieved in the observation group, and it was 5 and 18 cases in the control group, respectively (P<0.05). It spent (26.69±16.48) days to cure in the observa tion group, and (38.24±22.47) days in the control group (P<0.05). Conclusion The moist therapy may promote the cure of pressure ulcers af ter spinal cord injury, and shorten the time of cure.

Objective To analyze the main sources of dioxins pollution in fishes samples. Methods The principal components analysis was employed to identify different dioxin emission sources data and to analyze the environmental and aquatic samples. The Principal Component Analysis seems to be a powerful tool for source identification with the ratio of each concentration of the 2,3,7,8-subtituted congener to the concentration of the total 2,3,7,8-subtituted congeners. Results The result obtained from Principal Component Analysis showed that there were different dioxin compositions between chemical emission sources and other emission sources. Conclusion The pollution sources of PCDD and PCDF polluted aquatic life from different countries,regions are not the same.

Objective To explore the expressions of ER,PR and CerbB-2 in human papillary thyroid carcinoma,and their correlation with the clinical features.Methods The expressions of ER,PR and CerbB-2 were detected by immunohistochemistry in 89 papillary thyroid carcinoma samples and 13 nodular goiter samples.Results In papillary thyroid carcinoma samples,ER,PR and CerbB-2 were detected for 50.56 ％(45/89),43.82 ％ (39/89),24.72 ％ (22/89),respectively.In nodular goiter,ER,PR and CerbB-2 were detected for 15.38 ％ (2/13),7.69 ％ (1/13),0,respectively,there were significant differences between the two groups (x2 =5.649,P=0.017,x2 =6.211,P=0.013,x2 =4.097,P=0.043).The positive rates of PR were 56.26 ％(26/46) in without lymph node metastasis,compared with those lymph node metastasis 30.2 3％ (13/43) were significantly higher,the difference was statistically significant (x2 =6.239,P =0.012).Conclusion The expressions of ER,PR and CerbB-2 are significant higher in papillary thyroid carcinoma.The detection of ER,PR and CerbB-2 might be helpful for early diagnosis and prognosis of papillary thyroid carcinoma.

Objective To evaluate the effect of nerve block and nerve block combined with glycerol and fructose injection on cervicogenic headache (CEH).Methods 50 CEH patients were randomly divided into the nerve block group (n=25), received the occipital and C2 nerve block; nerve block combined with glycerol and fructose injection group (n=25), received nerve block plus glycerol and fructose injection. The pain degree (numeric rating scales, NRS) and the degree of cervical stiff measured by restriction of the range of motion of the neck (ROM) were recorded.Results NRS and ROM decreased significantly after treatment compared with the baseline in two groups ( P<0.01). NRS and ROM decreased much more in the nerve block combined with glycerol and fructose injection group than in the nerve block group ( P< 0.01).Conclusion Nerve block combined with glycerol and fructose injection is a more safe and effective method in the treatment of CEH.

Objective To isolate,cultivate and identify the human embryonic fibroblasts(hEFs) derived from the gonadal ridges and dorsal mesenteries of human embryos,and to detect the expression by hEFs of cytokines crucial for the growth of human embryonic germ cells(hEG) in vitro.Methods The hEFs were isolated by enzyme digestion from gonadal ridges and dorsal mesenteries of 5-to 9-week old human embryos.The cells were then cultivated.The biologic characteristics(morphology,growth characteristics and cell cycle) of these cells were also studied.The reverse transcription-polymerase chain reaction(RT-PCR) was used to seek the expressions of a specific fibroblast marker,prolyl 4-hydroxylase ?,and a specific marker of epithelial cells,cytokeratin-4,in the cells.The expressions of cytokines essential for the growth of hEG,namely basic fibroblast growth factor(bFGF) and leukemia inhibitory factor(LIF),were also examined by RT-PCR.Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos.They could be passaged beyond the 25th generation.The biologic characteristics of the cells did not change,even in high-passage cells or frozen-thawed cells.The cells expressed prolyl 4-hydroxylase ?, but not cytokeratin-4,which was similar to the fibroblasts.The cultured cells expressed bFGF and LIF.Conclusion The hEFs derived from gonadal ridges and dorsal mesenteries of human embryos are successfully isolated and cultivated,and the cells express the cytokines essential for the growth of hEG in vitro.

Objective This study examined the effects of Midazolum-ketamine oral solution (MKOS) on the expression of N-methyl-D-aspartate receptor 1 (NMDAR1) and gamma-aminobutyric acid (GABA)A receptors (GABAAR) mRNA in the cerebral cortex of rat, in order to investigate the sedation mechanism of MKOS. Methods Fifty Sprague-Dawley(SD) rats were divided into ten groups according to the observed time after MKOS administration (0,5,10, 15,30,60,90,120,240 and 360 minutes, n =5 each). The 0 minute group(control group) received 0.9% saline instead. Immunohistochemical staining and in situ hybridization were used to detect the expressions of NMDAR1 and GABAAR mRNA in the cerebral cortex. Results Both GABAAR and NMDAR1 all expressed in the glial cells of cerebral cortex. The expression of NMDAR1 in control group was strong. The expression of NMDAR1 became weaker during 15 to 90 minutes after administration of MKOS (P＜0.05). The expression of GABAAR mRNA in control group was weaker,while became stronger during 30 to 90 minutes after administration of MKOS (P ＜0. 05). Conclusion MKOS may play sedation by strengthening the expression of GABAAR and suppressing the expression of NMDAR1 in the cerebral cortex.

The daily intake levels and trend of polychlorinated dibenzo-p-dioxins(PCDDs), polychlorinated dibenzofurans(PCDFs), polychlorinated biphenyls(PCBs) of general population of several developed countries are reviewed. Based on the data of China, PCDD-Fs levels in Chinese human milk and blood were less than that of other countries, in addition, a daily intake of PCDD-Fs for Chinese general population was estimated in the present paper, the tolerable daily intake of Chinese general population was less than that the World Health Organization proposed.