Objective:To investigate the efficacy of dipeptidyl peptidase 4 inhibitor combined with insulin detemir (ID) in the treatment of type 2 diabetes mellitus with poor blood glucose control and its effects on blood glucose and oxidative stress indicators.Methods:A total of 105 patients with poor blood glucose control after taking oral hypoglycemic drugs who received treatment between August 2017 and September 2019 were included in this study. They were randomly assigned to receive treatment with either ID and compound vitamins (control group, n = 53) or dipeptidyl peptidase 4 inhibitor sitagliptin and ID (study group, n = 52). After 3 months of treatment, the clinical efficacy was compared between the control and study groups. Before and after 3 months of treatment, blood glucose indexes [fasting plasma glucose, 2-hour postprandial blood glucose], glycosylated hemoglobin, islet β-cell function [homeostasis model assessment-beta and homeostasis model assessment of insulin resistance)], oxidative stress index [superoxide dismutase, glutathione peroxidase, lipid hydroperoxides, malondialdehyde were compared between the two groups. The incidence of hypoglycemia was also recorded in each group. Results:After 3 months of treatment, total effective rate in the study group was significantly higher than that in the control group [92.31% (48/52) vs. 77.36% (41/53), χ2 = 4.541, P < 0.05]. After 3 months of treatment, fasting plasma glucose, 2-hour postprandial blood glucose, glycosylated hemoglobin, homeostasis model assessment of insulin resistance, lipid hydroperoxide and malondialdehyde levels in each group were significantly decreased, homeostasis model assessment-beta, superoxide dismutase and glutathione peroxidase levels were significantly increased compared with before treatment (all P < 0.05). There were significant significances in these indexes measured concurrently between the study and control groups ( t = 2.902, 3.539, 5.215, 7.313, 6.269, 4.475, 6.050, 3.323, 5.375, all P < 0.05). During the treatment, the incidence of hypoglycemia in the study group was significantly lower than that in the control group [0.00% (0/52) vs. 11.54% (6/53), χ2 = 4.319, P < 0.05]. Conclusion:Dipeptidyl peptidase 4 inhibitor combined with insulin detemir is highly effective for type 2 diabetes mellitus with poor blood glucose control. It can effectively control blood glucose level, improve oxidative stress reaction and is highly safe. Therefore, this treatment method is of good application value.

OBJECTIVE:To observe the effect of Tongxinluo capsule on the hemorheology of patients with coronary heart disease(CHD). METHODS:120 patients with CHD were randomly divided into 2 groups:60 case in the control group were as-signed to receive combined therapy plus Compound danshen tablets (3 tablets/time,tid),and 60 cases in treatment group to receive additional Tongxinluo capsules(3 capsules/ time,tid) in addition to the therapy applied for the control group. All patients were treated for 4 weeks during this period of time the lipid-lowering drugs and anticoagulants were discontinued. RESULT:The decreasing rates of hemorheology indexes were superior in the treatment group than in control group(P

Objective: To study on in vitro release of tetanus toxoid contained in polylactide microsp heres and the influence of additives on the drug release. Methods: Some parameters affecting the rate of release were asse ssed during the in vitro experiments lasted 84 days: (1)the molecular mass of the poly mers , (2)the protein loading of the microspheres, (3)the particle sizes, (4)co-enca p sulation of additives. Results: The polylactide microspheres con taining tetanus toxoid presented considerable sustained release effect. Release kinetics of the microspheres fitted in with zero order equations. The rate of release was shown to dep end on the four parameters mentioned above. Conclusion: The poly lactide microsph eres investigated may be prospective for the development of controlled release v accines.

At present, the prevalence of heart failure (HF) is still high in China, and its mortality and readmission rate have no downward trend. Therefore, the mechanism of its occurrence and development needs to be explored. In recent years, it has been found that about 13%-15% of HF patients have subclinical hypothyroidism (SCH), which can lead to cardiac systolic and diastolic dysfunction, thus promoting the occurrence and development of HF. At present, the curative effect of patients with mild elevation of thyroid stimulating hormone (TSH) level in HF patients with SCH is not clear, and a large number of prospective studies are still needed. In addition, in the complications of HF, SCH is the only disease that can reverse cardiac function after treatment. Therefore, this paper summarizes the mechanism of SCH in the occurrence and development of HF, and provides the basis for the formulation of clinical HF treatment strategies and the evaluation of prognosis.

Objective Toinvestigate the effect ofPBLmethod in the pediatrics'nurse teaching.Methods PBL teaching was practiced in trial group,while LBL teaching was practiced in control group.The writers made an analysis of this teaching by way of questionnaire and exam.Results Many students by PBL teaching accept PBL teaching,and improve their self-study ability,understanding ability,analyzing ability and so on.Conclusion PBL teaching method can effectively promote both the teaching quality in pediatric nursing and the students'study interest.

AIM:So far,endostatin is found playing the strongest effect to inhibit endothelial cells.Because endostatin protein is instable,it is difficult to prepare and apply.Gene therapy is used to assist endostatin to inhibit angiogenesis.This study constructed recombinant adenovirus of human endostatin gene using AdEasy-1 system to investigate the feasibility of expressing endostatin protein in ECV304 cells(human umbilical vein endothelial cells).METHODS:The experiment was performed at College of Life Science,Sun Yat-set University from March to October 2006.Endostatin was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector after sequencing and identification.The resultant plasmid was co-transduced into E.coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination.The recombinant plasmid was detected by restriction analysis and DNA sequence analysis.The linearized recombinant plasmid was transfected into AAV 293 cells with LipofectAMINE2000.The recombinant adenovirus was detected by GFP,PCR and restriction analysis.The virus was used to infect ECV304,and the expression of endostatin in vitro was detected by the techniques of RT-PCR and Western blot.RESULTS:The positive clones of the recombinants were constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis.AAV293 cells were transfected.The titer of viral liquid was 2.06?1010 pfu/mL.The transcription of endostatin mRNA in ECV304 cells was checked by RT-PCR and endostatin protein could be detected in the culture by Western blot analysis.CONCLUSION:The recombinant adenoviral vector pAd-Endo carrying human endostatin is successfully constructed,and effective expression is found in ECV304 cells in vitro.

The microvasculature of the rectus abdominis muscle in dogs was observed under scanning electron microscope. The specimens were treated by vascular corrosion east and enzymic digestive methods. Some new results had been obtained.The intramuscular arterioles penetrate the muscular fasciculus with almost right angle. The capillaries go along the myofibers oriented parallel to each other and arrange fascicularly. The morphologically distinct precapillary sphincters are apparent at the original region of the capillaries. There are abundant anastomosing channels between the capillaries which constitute ladder-like microvascular networks encircling the myofibers. Sometimes, the calibre was partial enlarged in the anastomosing channel, which may be beneficial to store of the blood oxygen.The physiological significance of the intramuscular microvasculature was discussed.

OBJECTIVE To observe the efficiency of new low-temperature sterilization method using(hydrogen) peroxide gas plasma sterilization system.METHODS The hydrogen peroxide gas plasma sterilization system of STERRAD 100S was monitored for 157 sterilization cycles.RESULTS From them 134 cycles were(completed,) 23 cycles were(cancelled.) All cycles were completed with negative results either in biological culture or in chemical indicators.CONCLUSIONS The STERRAD Sterilization System involves the combined use of hydrogen peroxide and low-temperature gas plasma to sterilize most medical devices and materials rapidly and safely without leaving any toxic residues.As nursing develops,the new sterilization system is efficient and convenient.However,it also has own limitations.Therefore,it is desirable to fully understand the normal procedure of system according to the operation instruction.

OBJECTIVE:To investigate the effect of mixing herbal injections with transfusion fluid on production of insoluble particles.METHODS:To detect the amount of insoluble particles before and after mixing herbal injections with transfusion fluid with ZWF-4 particule counter.RESULTS:The insoluble particles increased obviously.CONCLUSION:It should be paid great attention to the increase of insoluble particles after mixing,as well as the relationship between the insoluble particles and adverse reactions.

OBJECTIVE: To study the feasibility of using biodegradable chitosan nanoparticles as new type carriers for controlled GM-CSF delivery system. METHODS: GM-CSF and BSA loaded chitosan nanoparticles were prepared by ion cross linking method using tripolyphosphate as cross-linking agent with its morphology observed by transmission electron microscope. The encapsulating efficiencies of GM-CSF and BSA were determined respectively by fluorescence scanning and ultraviolet spectrophotometer. The in vitro drug release properties of chitosan nanoparticles were studied as well. RESULTS: Morphologically, the nanoparticles were spherical with mean particle size of 201nm. The encapsulating efficiencies of GM-CSF and BSA were 62.1% and 58.5%, respectively, and their in vitro accumulative drug release rates on the 3rd day were 69% and 82% respectively. CONCLUSION: The ion cross linking method is fit for the preparation of GM-CSF loaded chitosan nanoparticles.