Female ; Humans ; Hyperthyroidism ; drug therapy ; etiology ; Infant, Newborn ; Male

Aged ; Antibodies, Monoclonal, Murine-Derived ; therapeutic use ; Antigens, CD20 ; metabolism ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Bleomycin ; therapeutic use ; CD79 Antigens ; metabolism ; Cyclophosphamide ; therapeutic use ; Dacarbazine ; therapeutic use ; Doxorubicin ; therapeutic use ; Female ; Hodgkin Disease ; drug therapy ; metabolism ; pathology ; surgery ; Humans ; Lymphoma, Large B-Cell, Diffuse ; metabolism ; pathology ; surgery ; therapy ; Neoplasms, Second Primary ; metabolism ; pathology ; surgery ; therapy ; Prednisone ; therapeutic use ; Rituximab ; Vinblastine ; therapeutic use ; Vincristine ; therapeutic use

Curriculum reform is very important to an university.In the Pharmaceutical Analysis Department,the principal for a curriculum plays a positive role not only in the teaching,but also in the scientific research and the service for the society.

Objective Ultrasonongraphy and mammography were employed to estimate the pathological response of patients with breast cancer ,who had been accepted neoadjuvant chemotherapy .According to the pres-ent study ,we can provide additional evidence on therapeutic effect on evaluation of neoadjuvant chemotherapy and better selection of regime for breast cancer .Methods One hundred Thirty-six patients who were previously dia-gosed diagnosed with primary breast cancer were included in this study .All subjects were female with clearly pathological detection and accepted neoadjuvant chemotherapy about 4 to 6 cycles regardless of regime .The resid-ual tumor size was evaluated by mammography and /or ultrasonography before operation .Tumor size measured by image were compared with pathological size to predicting the accuracy of two types of imaging .Results Forty one of 116 records were undetectable imaging by mammogram and 19 of 106 records were undetectable by ultrasound which were considered a pathologic complete response .Sixty one(62.24%)of 98 patients who were accepted de-tection of mammogram and ultrasound would be predicted the tumor size by mammogram .Eighty three(84.69%) of 98 patients would be predicted the residual tumor by ultrasound .31 and 59 were accurately evaluated by mam-mogram and ultrasound , respectively .The result indicated that ultrasound was more accurate than mammogram (60.20%vs.31.63%,χ2 =16.11,P<0.001).The correctly rate was 92.85%(91/98)for ultrasound and 68. 37%(67/98) for mammogram.The diagnosis efficiency of ultrasound was more higher than mammogram ,even though there was no different significance between the two methods (χ2 =2.028,P=0.164).Conclusion Ultra-sonongraphy in estimating the residual tumor size after neoadjuvant chemotherapy of patients with breast cancer displays more accurately than mammography .

AIM:To construct AFT024-SCF cell line and HPC-Lhx2 cell line for confirming the biological function of AFT024-SCF.METHODS:The HPC-Lhx2 cell line, AFT024-SCF cell line and AFT024-GFP cell line were constructed by retro-viral infection.The expression of stem cell factor(SCF) in AFT024-SCF cells was detected by real-time PCR and Western blot.SCF in the supernatant of AFT024-SCF was detected with ELISA.The supernatant of AFT024-SCF and AFT024-GFP were collected and then diluted (1:10) with basic IMDM medium.So we made 4 culture medium:AFT024-SCF medium was used for experiment group, AFT024-GFP medium was used for endogenous negative control, IM-DM basic medium was used for exogenous negative control, and IMDM basic medium with SCF was used for positive con-trol.SCF-dependent HPC-Lhx2 cell line was cultured in these 4 different medium for 72 h.According to MTT method and colony forming experiment, the biological function of AFT024-SCF was confirmed by the proliferation ability of SCF-depend-ent HPC-Lhx2 cell line.RESULTS:SCF was highly expressed in AFT024-SCF cells.After cultured for 72 h, neither IM-DM basic medium nor GFP-AFT024 medium support HPC-Lhx2 cell line proliferation.However, AFT024-SCF medium supported HPC-Lhx2 cell line expansion as well as the positive control medium.CONCLUSION:AFT024-SCF cells ex-press SCF successfully and recombinant SCF can be replaced by the supernatant of AFT024-SCF culture medium for expan-ding HPC-Lhx2 cell line in vitro.

Ergosta-4,6,8(14),22-tetraen-3-one (ergone) is one of main components in many medicinal fungi. Ergone has been reported to possess the activities of diuresis, cytotoxicity, antitumor, immunosuppression, as well as treatment of chronic kidney disease. According to reported literatures, an overview of spectroscopy characteristics, content determination, pharmacological activity and pharmacokinetics, etc. for ergone is presented in this review. Furthermore, the present review can provide a certain reference value for the further study and development of ergone.
Animals ; Cholestenones ; chemistry ; pharmacokinetics ; pharmacology ; Drugs, Chinese Herbal ; chemistry ; pharmacokinetics ; pharmacology ; Humans

OBJECTIVETo investigate the role of p38 MAPK on ischemic postconditioning (IPO) attenuating pneumocyte apoptosis after lung ischemia/reperfusion injury (LIRI).

METHODSForty adult male SD rats were randomly divided into 5 groups based upon the intervention (n = 8): control group (C), LIR group (I/R), LIR + IPO group (IPO), IPO + solution control group (D), IPO + SB203580 group (SB). Left lung tissue was isolated after the 2 hours of reperfusion, the ratio of wet lung weight to dry lung weight (W/D), and total lung water content (TLW) were measured. The histological structure of the left lung was observed under light and electron transmission microscopes, and scored by alveolar damage index of quantitative assessment (IQA). Apoptosis index (AI) of lung tissue was determined by terminal deoxynuleotidyl transferase mediated dUTP nick end and labeling (TUNEL) method. The mRNA expression and protein levels of and Bax were measured by RT-PCR and quantitative immunohistochemistry (IHC).

RESULTSCompared with C group, W/D, TLW, IQA, AI and the expression of Bax of I/R were significantly increased, the expression of Bcl-2 and Bcl-2/Bax were significantly decreased (P < 0.05, P < 0.01), and was obviously morphological abnormality in lung tissue. Compared with I/R group, all the indexes of IPO except for the expression of Bcl-2 and Bcl-2/ Bax were obviously reduced, the expression of Bcl-2 and Bcl-2/Bax were increased (P < 0.05, P < 0.01). All the indexes between D and IPO were little or not significant( P > 0.05). The expression of Bcl-2 and Bcl-2/Bax of SB were significantly increased and other indexes were reduced than those of IPO (P < 0.05, P < 0.01).

CONCLUSIONIPO may attenuate pneumocyte apoptosis in LIRI by inactivation of p38 MAPK, up-regulating expression of Bcl-2/Bax ratio.

Alveolar Epithelial Cells ; cytology ; Animals ; Apoptosis ; Disease Models, Animal ; Ischemic Postconditioning ; Lung ; blood supply ; enzymology ; pathology ; Male ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; enzymology ; pathology ; prevention & control ; bcl-2-Associated X Protein ; metabolism ; p38 Mitogen-Activated Protein Kinases ; metabolism

Objective To evaluate PDCA circle in training of cooperation quality in endoscopic nurse.Methods PDCA circle was applied to train endoscopic nurses.Knowledge of endoscopic nursing and techniques as well as operators' satisfaction with endoscopic examination were compared before and after PDCA circle training.Results Nursing scores increased from 92.40 to 96.00 with statistical significance (P ＜ 0.05).Performance scores increased from 93.03 to 98.13 (P ＜ 0.05).Operators' satisfaction increased from 81.3％ to 96.9％.Conclusion PDCA circle could improve not only endoscopic nursing but also the quality of endoscopic training.

Objective To investigate the normal ranges of RET-He and reticulocyte grouping parameters of healthy adults in Wenzhou. Methods A total of 1 000 samples from healthy adults were collected in Wenzhou from August 2008 to February 2010. There were 506 males and 494 females. The individuals were stratified based on age into three groups:20 to 40-year-old group (n = 341 ), ＞40 to 60-year-old group (n =316), and ＞60-year-old group (n =343). All blood samples were drawn in EDTA-K2 anticoagulated blood. Peripheral RET-He and reticulocyte grouping parameters ( IRF, LFR, MFR, HFR)were determined by Sysmex XE-2100 automated hematology analyzer. The instrument was calibrated and validated before testing All specimens were analyzed within 2 hours. RET-He, RET absolute value and RET percentage were normal distribution, the normal range was established by using ((x)-1.96s,(x)+ 1.96 s)method. IRF, LFR, MFR and HFR data were skewed distribution,the normal range was established by using (P2.5 ,P97.5 )percentile method. Results The mean and normal range of RET-He were (33.91 ± 1.77) pg and (30.44 - 37.38) pg in male, (33.97 ± 1.85) pg and (30.34 - 37.60) pg in female,respectively. There was no significant difference between the two groups ( t =-0.533, P＞0.05 ). The mean and normal range of RET absolute value were (0.051±0.023) × 1012/L and (0.006 -0.096)×1012/L in male,which were (0. 037 ±0. 026) × 1012/L and (0 -0. 088) × 1012/L in female, there was significant difference between the two groups ( t = 8. 409, P ＜ 0. 05 ) . The mean and normal range of RET percentage in male were(1.041 ±0.459)% and (0. 141 -1.941)% , and in female were (0.869±0.603)% and (0-2. 051 )%, which showed significant difference (t =5.074,P ＜0. 05). The normal ranges of LFR,MFR,HFR,IRF in male were 0. 951 (0.866 -0.988) ,0.046(0.010 -0.114) ,0. 003(0 -0.023) ,0. 050(0.012-0.134), and in female were 0.096(0.880 -0.991) ,0.039(0.008 -0.113) ,0.002(0-0.016) ,0.040(0.009-0.121), respectively. There were significant differences between the two groups (Z values were -5.044, -4.793, -3.559, -5.075, respectively,all P＜0.05). The means of RET-He in 20 to 40-year-old group, ＞40 to 60-year-old group, ＞ 60-year-old group and all age group were (33.38±1.49),(34.36 ±1.46), (34.12±2.21) and (33.94 +1.81) pg, respectively. The normal ranges were(30.46-36.30), (31.50 -37.22), (29.79 - 38.45) and (30.39 - 37.49) pg, which showed statistical difference( F =28. 072,P ＜0. 05). In addition, the normal range of RET-He in 20 to 40-year-old group was lower than that of ＞ 40 to 60-year-old group and ＞ 60-year-old group ( q values were 9. 970, 7. 791,respectively, P＜0. 05). The normal ranges of LFR, MFR, HFR, IRF in ＞ 60-year-old group were 0.961(0. 878 -0. 992) ,0. 037(0. 007 -0. 105 ) ,0. 002(0 -0. 020) and 0. 039(0. 008 -0. 117) ,while they were 0.953(0. 867 -0.990), 0.045(0.009 -0. 116) ,0.003(0 -0.019) ,0. 050(0. 010 -0. 133) in 20 to 40 -year-old group , and 0. 951 (0.855 -0.989) ,0.047 (0.010 - 0.133 ) ,0.003 ( 0-0.020), 0.049 (0.011-0.149)in ＞40 to 60-year-old group, which showed statistical difference (Z values were -3. 949, -4.236,-4. 373, -4. 973, - 2. 747, - 3. 275, - 3.901, - 4. 185, respectively, all P ＜ 0. 05). There was nodifference between male and female for RET-He, the same normal ranges could be used, but there was significant difference between different age groups. LFR, MFR, HFR and IRF were statistically different between different age groups and different sex groups. The normal ranges should be established by gender and age. Conclusion The normal ranges of RET-He and reticulocyte grouping parameters about healthy adults in Wenzhou are established, which plays an important role in clinical value of RET parameters.

Objective To study the expressions of programmed death 1 (PD-1) and programmed death ligand 1 (PD-L1) in liver injury of acute liver failure (ALF) in rats and the role of PD-1/ PD-L1 in liver inflammatory injury. Methods SD rats were divided into two groups: 6 in normal group and 30 in ALF model group. The ALF models in rats were induced by D-galactosamine (D-Gal). The sera and hepatic tissue samples were collected at 12, 24, 48, 72 and 120 hours after D-Gal injection. Expressions of PD-1 mRNA and PD-L1 mRNA in hepatic tissue samples were detected by reverse transcription-polymerase chain reaction (RT-PCR). Comparison of measurement data between groups was done by t test. Correlation test was performed using Pearson linear correlation analysis. Results The levels of alanine animotransferase (ALT) and aspartate animotransferase (AST) at 12 h of D-Gal injection were (217. 3±33. 7) U/L and (397. 2± 101.3) U/L, respectively,which were both significantly higher than those in normal group [(30. 5 ±3. 1) U/L and (78. 6±4.2) U/L, respectively; t=-8. 921 and -6. 121, respectively; both P＜0.01] and peaked at 48 h.The expression of PD-1 mRNA in model group at 12 h (0. 385±0. 074) was significantly higher than that in normal group (0. 097±0.009) (t= -7. 725, P＜0.01) , and peaked at 48 h (0. 927±0. 132),then decreased obviously at 72 h. The expression of PD-L1 mRNA in the liver tissue of normal rats was very little, while that in model group was increased gradually over time, then peaked at 48 h (0. 593±0. 105; t =- 10. 076, P＜0. 01). The expressions of PD-1 and PD-L1 were positively correlated with ALT level (r=0. 807 and 0. 792, respectively; both P＜0. 01). Conclusion The expressions of PD-1/PD-L1 may play an important role in liver inflammatory injury in rat model of acute liver failure.