A total of 65 patients of condyloma acuminatum (CA) received cryotherapy plus 5-aminolevulinic acid-photodynamic therapy (ALA-PDT) or ALA-PDT alone.During cryotherapy,handling was gentle with a moderate depth.The nursing of PDT was both meticulous and comprehensive.Patients with adverse reactions received Kangfuxin liquid for wet packing and topical antibiotic ointment.The combination group received cryotherapy for 2 sessions and then PDT for 1-3 sessions while PDT was administered for 1-4 sessions in single PDT group.Then all patients received PDT once for consolidation therapy.For groups 1 and 2,the effective rates were 83％ and 70％ and the recurrent rates 17％ and 30％ respectively.Cryotherapy plus ALA-PDT is feasible for CA and it offers better efficacies and lower recurrent rates.And comprehensive,meticulous and comfortable nursing may achieve clinical efficacies,reduce the recurrent rate and minimize discomforts during treatment.

This study was aimed to explore the compatible mechanism of Shanyao, Fuling and Baishu in traditional Chinese medicine (TCM) cosmetology. Based on compatible principles, combination characteristics and modern relat-ed researches, the authors made an in-depth analysis on the modern scientific meaning and clinical application of Shanyao, Fuling and Baishu. The results showed that the compatibility of Shanyao, Fuling and Baishu was mainly used as a compatible group in TCM cosmetology. It was concluded that the compatible mechanism of Shanyao, Fuling and Baishu was flexible, which can be widely used in the clinical practice.

OBJECTIVE To observe the efficiency of new low-temperature sterilization method using(hydrogen) peroxide gas plasma sterilization system.METHODS The hydrogen peroxide gas plasma sterilization system of STERRAD 100S was monitored for 157 sterilization cycles.RESULTS From them 134 cycles were(completed,) 23 cycles were(cancelled.) All cycles were completed with negative results either in biological culture or in chemical indicators.CONCLUSIONS The STERRAD Sterilization System involves the combined use of hydrogen peroxide and low-temperature gas plasma to sterilize most medical devices and materials rapidly and safely without leaving any toxic residues.As nursing develops,the new sterilization system is efficient and convenient.However,it also has own limitations.Therefore,it is desirable to fully understand the normal procedure of system according to the operation instruction.

Objective To evaluate the role of autophagy in apoptosis in cortical neurons of rats in the early phase of oxygen-glucose deprivation (OGD).Methods Female Sprague-Dawley rats at 16-18 days of gestation,weighing 350-400 g,were sacrificed after being anesthetized with pentobarbital sodium.The fetal rats were obtained and the primary cortical neurons were isolated and seeded in culture plates.The neurons were then divided into 4 groups (n =7 each) using a random number table:control group (group C),OGD 30 min group,OGD 1 h group,OGD 2 h group,and OGD 3 h group,OGD + autophagy inducer rapamycin group (OGD + R group),and OGD + autophagy inhibitor 3-methyladenine (3-MA) group (OGD + 3-MA group).The neurons were incubated in sugar-free DMEM medium containing 5％ CO2 and 95％ N2 for 30 min,1,2 and 3 h in OGD 30 min,1 h,2 h,and 3 h groups,respectively.Rapamycin (final concentration of 100 nmol/L) and 3-MA (final concentration of 10 mmol/L) were added to sugar-free DMEM medium in OGD + R and OGD + 3-MA groups,respectively,and the neurons were then incubated for 1 h.The CCK-8 assay was used to detect the neuronal viability after the end of OGD.The expression of caspase-3 and LC3 Ⅱ was measured by Western blot.Results Compared with C group,the survival rates of cortical neurons were significantly decreased in the other groups,the expression of LC3 Ⅱ was up-regulated in OGD 30 min,1 h,2 h and 3 h groups,no significant change was found in caspase-3 expression in OGD + R group,and the expression of caspase-3 was up-regulated in the other groups.Compared with OGD 1 h group,the survival rates of cortical neurons were significantly decreased in OGD2 h,OGD3 h and OGD + 3-MA groups,the survival rates of cortical neurons were increased,and the expression of caspase-3 was down-regulated in OGD + R group,the expression of LC3 Ⅱ was up-regulated in OGD + 3-MA group,and the expression of LC3 Ⅱ was down-regulated in OGD2 h and 3 h groups.Conclusion Autophagy initiated in the early phase of OGD can inhibit apoptosis in cortical neurons of fetal rats.

This study was aimed to analyze the influence of Dioscorea opposita Thunb. by processing methods from the aspect of formula. The search was made on formulae containing Dioscorea opposita Thunb. from the classic book For-mula Dictionary of Traditional Chinese Medicine. Processing methods were analyzed and shown with charts by EXCEL. The results showed that the most frequently used processing methods for Dioscorea opposita Thunb. were stir-heating, dry and crude in the clinical practice. Among them, the stir-heating Dioscorea opposita Thunb. was good at reinforcing the spleen and the kidneys. The crude produce was good at tonifying the lungs. And the dry product was good at rein-forcing the kidneys. It was concluded that the processing methods had obvious influence on the clinical function of Dioscorea opposita Thunb., which can be used as reference of more value for the clinics and health cultivation.

Objective To investigate the nursing knowledge of rural patients with rectal carcinoma after permanent colostomy.Methods Data were collected on 54 patients with rectal carcinoma after permanent colostomy using the self-designed questionnaire and analyzed by statistical methods.Results The rural patients with permanent colostomy showed low level of nursing knowledge.Scores of patients with different sexes and family income showed significant difference.Conclusions Medical security system and medical staff should provide with more welfare policy and specialty support for the patients in rural areas to promote their knowledge of self-care.

Chronic inflammation,oxidative stress and retinal ganglion cell apoptosis play important roles in the development of diabetic retinopathy.Fenofibrate,a peroxisome proliferator-activated receptor α agonist,is used for dyslipidemia.In addition to its lipid-modulating effects,fenofibrate also has antiinflammatory,antioxidant,anti-apoptotic and anti-angiogenesis properties that may be useful to delay the progression of diabetic retinopathy.Some clinical studies have already confirmed that fenofibrate has therapeutic effect on diabetic retinopathy.Further studies the application of fenofibrate in the treatment of diabetic microangiopathy to clarify the safety and efficacy of fenofibrate is of great significance.

Objective To express the recombinant antigen coded by Pagumogonimus skrjabini adult cysteine protease gene fragment and to investigate the immunoreactivity of the fusion protein for further usage on serodiagnosis of the fluke disease. Methods The target gene fragment was amplified by PCR. After purification with gel purification recoverykit, the target gene fragment was ligated with PinPointTM Xa 1 T vector and transduced into E.coli JM109 strain. The expressed fusion protein sample was prepared with alkaline lysis solution, and then analyzed by SDS PAGE. The expression level was determined by Coomassie blue staining and the streptavidin alkaline phosphatase staining. The immunoreactivity of fusion protein was examined by Western blotting. Results A total of 8 positive clones were harvested, and only one had the proper orientation verified by sequencing. The recombinant antigen was obtained after being induced with IPTG (Isopropltio ? D galactoside), and a positive band of 32?10 3 was found by streptavidin alkaline phosphatase staining. In the same position, the fusion protein was also detected by Western blotting. Conclusion The expression vector of adult cysteine protease gene PinPointTM Xa 1 T vector was successfully constructed. The recombinant antigen obtained after being induced with IPTG possesses good immunoreactivity.

Objective To compare the value of transrectal real -time elastography ( TRTE) with dynamic contrast material -en-hanced magnetic resonance imaging (DCE-MRI) for prostate cancer detection.Methods A total of 60 men (mean age 71years, range 43 to 83) with serum PSA levels of greater than 4.0ng/ml were assessed using gray -scale transrectal ultrasound (TRUS), transrectal real-time elastography ( TRTE ) and dynamic contrast material -enhanced magnetic resonance imaging ( DCE -MRI ) .Subsequently , these patients underwent systematic sextant transrectal biopsy and additional biopsies for positive sites on gray -scale TRUS, TRTE and DCE-MRI.The cancer detection rates of the 3 techniques were compared .Results Cancer was detected in 23 of the 60 patients (23/60,38.3%).TRTE and DCE-MRI had a higher sensitivity, specificity and accuracy of 73.9%,78.3%,87.0%,80.1%,75.1%, 89.2%,78.3%,76.7%,88.3%respectively.TRTE and DCE-MRI guided targeted biopsy improve the positive detection rate of pros-tate cancer, compared with TRUS (P<0.05).Conclusion TRTE and DCE-MRI can improve the detection rate of the increase of ser-um PSA in patients with prostate cancer , so as to improve the positive rate of prostate biopsy diagnosis ,provide strong support for clinical diagnosis

[Summary] From February 2008 to June 2008, two cases of old ectopic pregnancy were misdiagnosed: Case 1 was misdiagnosed as gestational trophoblastic disease (GTD), and Case 2 was misdiagnosed as cornual pregnancy.In order to further confirm the diagnosis, two cases were all given laparoscopic exploration.Surgery confirmed interstitial chronic ectopic pregnancy, leading to a resection of the fallopian tubes and mass lesions.Embryo tissue was pathologically confirmed postoperatively.The diagnosis of old ectopic pregnancy is difficult.Serum hCG determination is often found no positive, and ultrasound examination has no specificity.The clinical images are varied.Detailed medical history inquiry and attention to differential diagnosis are key for the diagnosis of old ectopic pregnancy.