1.A Neural Circuit Mechanism Controlling Breathing by Leptin in the Nucleus Tractus Solitarii.
Hongxiao YU ; Luo SHI ; Jinting CHEN ; Shirui JUN ; Yinchao HAO ; Shuang WANG ; Congrui FU ; Xiang ZHANG ; Haiyan LU ; Sheng WANG ; Fang YUAN
Neuroscience Bulletin 2022;38(2):149-165
Leptin, an adipocyte-derived peptide hormone, has been shown to facilitate breathing. However, the central sites and circuit mechanisms underlying the respiratory effects of leptin remain incompletely understood. The present study aimed to address whether neurons expressing leptin receptor b (LepRb) in the nucleus tractus solitarii (NTS) contribute to respiratory control. Both chemogenetic and optogenetic stimulation of LepRb-expressing NTS (NTSLepRb) neurons notably activated breathing. Moreover, stimulation of NTSLepRb neurons projecting to the lateral parabrachial nucleus (LPBN) not only remarkably increased basal ventilation to a level similar to that of the stimulation of all NTSLepRb neurons, but also activated LPBN neurons projecting to the preBötzinger complex (preBötC). By contrast, ablation of NTSLepRb neurons projecting to the LPBN notably eliminated the enhanced respiratory effect induced by NTSLepRb neuron stimulation. In brainstem slices, bath application of leptin rapidly depolarized the membrane potential, increased the spontaneous firing rate, and accelerated the Ca2+ transients in most NTSLepRb neurons. Therefore, leptin potentiates breathing in the NTS most likely via an NTS-LPBN-preBötC circuit.
Leptin/pharmacology*
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Membrane Potentials
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Neurons/metabolism*
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Solitary Nucleus/metabolism*
2.Circuit-Specific Control of Blood Pressure by PNMT-Expressing Nucleus Tractus Solitarii Neurons.
Shirui JUN ; Xianhong OU ; Luo SHI ; Hongxiao YU ; Tianjiao DENG ; Jinting CHEN ; Xiaojun NIE ; Yinchao HAO ; Yishuo SHI ; Wei LIU ; Yanming TIAN ; Sheng WANG ; Fang YUAN
Neuroscience Bulletin 2023;39(8):1193-1209
The nucleus tractus solitarii (NTS) is one of the morphologically and functionally defined centers that engage in the autonomic regulation of cardiovascular activity. Phenotypically-characterized NTS neurons have been implicated in the differential regulation of blood pressure (BP). Here, we investigated whether phenylethanolamine N-methyltransferase (PNMT)-expressing NTS (NTSPNMT) neurons contribute to the control of BP. We demonstrate that photostimulation of NTSPNMT neurons has variable effects on BP. A depressor response was produced during optogenetic stimulation of NTSPNMT neurons projecting to the paraventricular nucleus of the hypothalamus, lateral parabrachial nucleus, and caudal ventrolateral medulla. Conversely, photostimulation of NTSPNMT neurons projecting to the rostral ventrolateral medulla produced a robust pressor response and bradycardia. In addition, genetic ablation of both NTSPNMT neurons and those projecting to the rostral ventrolateral medulla impaired the arterial baroreflex. Overall, we revealed the neuronal phenotype- and circuit-specific mechanisms underlying the contribution of NTSPNMT neurons to the regulation of BP.
Solitary Nucleus/metabolism*
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Blood Pressure/physiology*
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Phenylethanolamine N-Methyltransferase/metabolism*
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Neurons/metabolism*
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Paraventricular Hypothalamic Nucleus/metabolism*