The chemical constituents of fistular onion stalk obtained by supercritical CO(2) extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained. On the basis of the spectral data, they were structurally identified as (+)-catechin, (-)-epicatechin, astragalin, and 3-O-beta-D(2-O-beta-D-glucopyranosyl)-glucopyranosides of kaempferol.

OBJECTIVETo study the cause of the seeds dormancy of Glehnia littoralis in vitro and to establish plant regeneration methods via somatic embryos.

METHODThe effects of endosperm and exogenous hormone on the seed dormancy breaking of G. littoralis and the effect of hormone concentration on embryonic callus induction and plant regeneration via somatic embryos were observed,

RESULTSThe germination rate of the seeds with 1/3 endosperm was the highest which achieved 31%. TDZ, 6-BA and GA3 treatment could not break seed dormancy but easily lead to abnormal seedlings. Embryogenic callus induction rates was up to 57% on MS supplemented with 1.0 mg x L(-1) 2,4-D. After 20 days culture, embryogenic calli were transferred to MS medium and cotyledonary embryos were formed in 40 days. The regenerated plants were obtained in 20 days.

CONCLUSIONAn effective system of plant regeneration of G. littoralis was established in this study.

Apiaceae ; physiology ; Endangered Species ; Plant Somatic Embryogenesis Techniques ; Plants, Medicinal ; physiology ; Regeneration ; Seeds ; physiology

The chemical constituents of fistular onion stalk obtained by supercritical CO2 extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained.On the basis of the spectral data,they were structurally identified as(+)-catechin,(-)-epicatechin,astragalin,and 3-O-β-D(2-O-β-D-glucopyranosyl)-glucopyranosides of kaempferol.