1.Changes in Cell-mediated Immune Function among Older Inpatients with Pseudomonas aeruginosa Pneumonia
Chinese Journal of Nosocomiology 1994;0(04):-
OBJECTIVE To study the cell-mediated immune function among older inpatients with Pseudomonas aeruginosa pneumonia.METHODS T-cell subsets and expression of membrane molecule on activated T-cell in peripheral blood were measured in 35 cases with P.aeruginosa pneumonia by using immunofluorescence and flow cytometry,and compared with those of control healthy older people.RESULTS The expressions of CD4+ /CD3+ and CD25+ /CD3+ in P.aeruginosa pneumonia patients were lower than in control group(P0.05).CONCLUSIONS There are disfunctions of lymphocgte and immunity among P.aeruginosa pneumonia patients.
2.Analysis on Application Rationality of Anti-fungal Agents for Deep Fungal Infection in Inpatients in a Top Three Hospital
Ping WANG ; Xiujie LI ; Tao YIN ; Min JIN
China Pharmacist 2014;(2):290-292
Objective:To investigate the rationality of anti-fungal agents for deep fungal infection used in one hospital and the effects of national rectification of antimicrobial drugs. Methods: The retrospective analysis method was used to survey the inpatients administrated anti-fungal agents for deep fungal infection from April 2010 to Mard 2012, and assessed the rationality. Results:The ir-rational utilization of anti-fungal agents for deep fungal infection included loading dosage lack during the treatment,inappropriate loading dosage and administration frequency. The irrational utilization of anti-fungal agents for deep fungal infection was decreased significantly (P<0. 05)after the national rectification of antimicrobial drugs. Conclusion:After the national rectification of antimicrobial drugs, the hospital can amtrol the irrational use of anti-fungal agents to some extent, while still needs more management and education.
3.Research achievements on structures and activities of polysaccharides from Panax ginseng.
Shan-shan LI ; Yin-ping JIN ; Chun-lin YAO ; Ying-ping WANG
China Journal of Chinese Materia Medica 2014;39(24):4709-4715
Panax ginseng C. A. Meyer (P. ginseng) has been used as traditional medicine in Asian countries for more than 2,000 years. P. ginseng contains many active components such as ginsenosides, peptides, essential oil and polysaccharides, among which, P. ginseng polysaccharides were reported to have immunomodulating, anti-cancer, anti-adhesive and antioxidant activities. For better understanding of the structures and biological activities of all the ginseng polysaccharides, here the recent research achievements were reviewed. This review would be helpful for the relevant researchers to get useful information.
Antineoplastic Agents
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analysis
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isolation & purification
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pharmacology
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Antioxidants
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analysis
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isolation & purification
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pharmacology
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Humans
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Immunologic Factors
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analysis
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isolation & purification
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pharmacology
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Medicine, Traditional
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Panax
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chemistry
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Plant Roots
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chemistry
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Plants, Medicinal
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Polysaccharides
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analysis
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isolation & purification
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pharmacology
4.Strategy to delay the progression of chronic kidney disease by targeting gut microbiota and uremic toxins metabolism pathway
Yin PENG ; Xue-jun XU ; Jian-ping LI ; Cheng-xi LI ; Jia-ting YIN ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2021;56(1):37-49
Chronic kidney disease (CKD) is a serious chronic disease with high incidence, poor prognosis, and a variety of complications. Indoxyl-sulfate (IS) and
5.Overexpression of HIV-1 P17 protein in Escherichia coli and its purification
Zhongzhu LI ; Ningyi JIN ; Hongwei WANG ; Zhiru GUO ; Ping LI ; Hangyi YANG ; Zhen YIN
Journal of Jilin University(Medicine Edition) 2001;27(1):19-21
Objective:To highly express HIV-1p17 in E.coli and purify the protein.Methods:①Recombinant plasmid was constructed by inserting HIV-1p17 gene amplified by PCR into plasmid vector,pET28c;②The recombinant plasmid was expressed in BL21,BL21(DE3),BL21(DE3) plysS and HMS174(DE3) of E.coli separately;③The target protein were purified with Ni-NTA resin;④The purified protein was detected by western blot and ELISA.Results:The expression of the P17 protein in BL21(DE3) represented up to 32% of total protein in E.coli,which was the most amounts compared with other kinds of E.coli.The purity of the purified protein reached 95%.The purified protein was recognized by HIV-1P17McAb as well as by HIV-1 positive serum.Conclusion:The recombinant plasmid is highly expressed in BL21(DE3) of E.coli that can be proceeded to the immunocompetence and the bioactivity research.The method of Ni-NTA resin is simple with low protein losing and high purity.And the purified p17 can be employed in early detection of HIV-1 infection and prediction of the clinical progression.
6.Analysis of health education effects on integrated program for controlling endemic fluorosis in Guizhou Province
Yin, LIANG ; Dong, AN ; Ping, HE ; Da-sheng, LI ; Zheng-jing, JIN ; Xiao-qiang, HU
Chinese Journal of Endemiology 2008;27(2):216-219
Objective To evaluate the effects of implementing the health education in the Central Fund Program to control endemic fluorosis in Guizhou.Methods The samples were randomly surveyed to evaluate knowledge awareness in students and households as well as the habit formation after implementing the integrated program which mainly consisted of installing the improved stoves,supported by the Central Funds and health education in 5 counties.Results After health education,the rates of knowledge awareness in the students and the households were 94.80%(15 562/16 415)and 88.23%(4482/5080),respectively,and increased significantly compared with those before intervention[44.20%(26 364/59 645),22.81%(3082/13 510)],the difference being significant(χ2=13 324.05,6546.24,P<0.01).The rates of drying corn and chili with the coal fire were 5.61% (57/1016)and 5.41%(55/1016),respectively,and decreased significantly compared with those before intervention [77.41%(1076/1390),78.92%(1097/1390)],the difference being significant(χ2=1214.49,1270.92,P<0.01).The rates of washing corn and chili were 99.51%(1011/1016)and 94.59%(961/1016),respectively,and increased significantly compared with those before intervention[84.60%(1176/1390),76.55%(1064/1390)],the difference being significant(χ2=154.80,143.32,P<0.01).The rates of using the uncovered and unventilated iron stoves and table stoves were 4.71%(38/807)and 8.37%(60/717),respectively,and decreased significantly compared with those before intervention[29.99%(14 483/48 299),98.33%(95 070/96 685)],the difierence being significant(χ2=243.51,25 282.99,P<0.01).Conclusions Implementing the health education is the basis for the integrated measures for controlling the endemic fluorosis in the endemic regions.The consciousness and activity of the target people have been enhanced greatly.The good behaviors in the target people are forming,the expected goal is reached.
7.Influence of stove an d coal-burning mode on formation of severe endemic fluorosis regions in Guizhou Province
Da-sheng, LI ; Dong, AN ; Ping, HE ; Yin, LIANG ; Zheng-jing, JIN
Chinese Journal of Endemiology 2008;27(2):183-187
Objective To explore the influence of stoves and mode of coal-burning on the prevalence of endemic fluorosis in Guizhou Province.Methods Three previous severe endemic villages and two mild villages(as control)were investigated by ways of random sampling in the present and retrospect epidemiological study on stove usage,coal burning mode and the severity of disease.Results Before the 1990's Of 20th century all the households in the previous severe endemic villages used the open stove without ventilated pipe(chimney)and dehydrated the corn over the coal-fire of the open stoves in house,which resulted in severe pollution of the corn and indoor air by fluoride.After the 1990's,each household,although still dried the corn with the coal fire,used the table stoves and iron stoves increasingly year by year,which resulted in the decrease of the emitted fluoride from the coal fire significantly,reduced the fluoride pollution of the indoor air and the main food,thus the incidence of endemic fluorosis consequently decreased. On the other hand, most of the households in the previous severe endemic villages such as Lianhe and Yuanzi still used the open, unventilated stove without chimney, very few installed chimney leading to outside of the room,the rates being 51.3%(41/80)and 41.7%(35/84),respectively.It was concordant to the high incidence of endemic fluorosis.Compared with 1979,the detective rates of dental fluorosis in the crowd aged above 8 years old were increased significantly in Hehua Village in 2006,90.51%(248/274)and 96.08%(392/408),respectively(χ2=7.85,P<0.01).Conclusions The usage of stoves and mode of coal-burning significantly influence the incidence of the endemic fluorosis caused by domestic coal-burningin Guizhou Province,and inappropriated use of stove and the mode of coal burning are the key factors in cause the prevalence of the severe fluorosis in endemic regions in Guizhou.
8.Effect of food drying methods on fluoride content in maize and pepper in coal-burning type of fluorosis regions
Nian-heng, ZHANG ; Dong, AN ; Ping, HE ; Da-sheng, LI ; Yin, LIANG ; Zheng-jing, JIN
Chinese Journal of Endemiology 2010;29(5):536-539
Objective To explore the effect of different processing and drying methods of corn and hot pepper on fluorine content in coal-burning type of the endemic fluorosis areas, and to screen food processing and drying methods which meet the quality requirements of grain drying and able to effectively reduce the total fluoride intake of local population. Methods Farmers of endemic fluorosis area in Bijie, Guizhou province were divided into 3 groups: sun-baked drying group, stove drying group with air-tight cover and stove drying group with no cover, 10 households in each group. Corn and fresh hot pepper and samples dried for 2 weeks, or 1, 3, 6-month were collected, and water and fluoride content were detected, and the total daily fluoride intake were calculated in accordance with the "Determination of Water in Food" (GB/T 5009.3-2003) and "Determination of Fluorine in Foods"(GB/T 5009.18-2003). Results Fluoride content in fresh corn and dried for 2 weeks, or 1, 3, 6-month [of sunbaked drying group: (1.40 ± 0.16), (1.56 ± 0.14), (2.15 ± 0.47), (2.70 ± 0.64), (4.06 ± 1.75)mg/kg, stove drying group with air-tight cover: (1.41 ± 0.16), (2.39 ± 0.56), (4.60 ± 0.97), (8.46 ± 5.55), (11.36 ± 3.60)mg/kg,stove drying group with no cover: (1.40 ± 0.13), (4.69 ± 3.97), (4.47 ± 2.77), (9.65 ± 6.47), (26.12 ± 14.52)mg/kg] and pepper[sun-baked drying group: (5.41 ± 1.61), (16.60 ± 7.62), (32.60 ± 7.88), (50.26 ± 17.60),(240.20 ± 272.49)mg/kg, stove drying group with air-tight cover: (754 ± 2.95), (3238 ± 11.50), (119.18 ± 156.45),(224.00 ± 196.58), (495.70 ± 417.29)mg/kg, stove drying group with no cover: (4.82 ± 1.25), (44.30 ± 13.48),(122.89 ± 66.43), (334.23 ± 166.05), (531.01 ± 397.40)mg/kg] increased with elongation of drying time, and the group difference was significant(F = 44.77, 128.71, 126.87, 41.61, 53.63, 170.63, all P < 0.05), with the largest rate of increase in stove drying group with no cover, and the lowest in sun-baked drying group;fluoride was significantly lower (t = 7.93,63.07,5.36,11.98,55.76,7.45, all P < 0.05) after sample washing;total fluoride intake per person per day was 2.57 mg in local adult when ate washed and sun-baked corn, peppers, the total fluoride intake were 5.92, 8.14 mg when ate the food processed by other two drying methods and washed corn, peppers,respectively. Conclusions In the coal-burning type of fluorosis endemic area, should take appropriate health education measures, and instruct local residents to use sun bake their edible corn and pepper for human consumption, and cultivate a habit of washing corn and pepper before cooking, which can reduce the population total fluoride intake, and control endemic fluorosis.
9.Status on Heart Transplantation in China.
Xing-Jian HU ; Nian-Guo DONG ; Jin-Ping LIU ; Fei LI ; Yong-Feng SUN ; Yin WANG
Chinese Medical Journal 2015;128(23):3238-3242
10.Expression of B lymphocyte stimulator in peripheral blood mononuclear cells in individuals with systemic lupus erythematosus and the role of interferon-? on it's expression
Yu-Jin YE ; Han-Shi XU ; Liu-Qin LIANG ; Pei-Da YIN ; Xiu-Yan YANG ; Zhong-Ping ZHAN ; Fan LIAN ;
Chinese Journal of Rheumatology 2003;0(10):-
Objective To determine the expression of membrane-bound B lymphocyte stimulator (BLyS) protein and its mRNA in vitro of peripheral blood mononuclear cells (PBMCs) from individuals with systemic lupus erythematosus (SLE),and to investigate the role of interferon-?(IFN-?) on the expression of BLyS.Methods PBMCs were obtained from 25 SLE patients (mean age of 31+14) and 20 healthy volunteers (mean age of 28?10).They were randomized into IFN-?(5 ng/ml) group and control group.PBMCs were col- lected at 0,6,12 and 24 h for BLyS mRNA assessment using semi-quantitative reverse transcription-PCR (RT-PCR).PBMCs were also collected at 72 h for membrane-bound BLyS protein detection using flow cy- tometry (FACS) and direct immunofluorescence.Results①The expression of BLyS mRNA and membrane- bound protein in PBMCs was significantly higher in individuals with SLE compared with healthy controls (P<0.05);②IFN-?enhanced BLyS mRNA expression in PBMCs in both healthy controls and SLE patients,with the greatest effect at 6 h (stimulated vs unstimulated,0.42?0.19 vs 0.25?0.14,P<0.01;0.59?0.28 vs 0.44?0.21,P<0.01 );③IFN-?also increased the expression of membrane-bound BLyS protein in both healthy con- trols and individuals with SLE (FACs,mean fluorescence intensity,4.5+3.0 vs 3.7~2.6,P