OBJECTIVE: To estimate the value of nasal endoscopic dacryocystorhinostomy combined image guidance system in treating chronic dacryocystistis. METHOD: Thirteen cases (14 eyes) performed surgeries with nasal endoscopic dacryocystorhinostomy combined image guidance system from January 2010 to August 2013 were retrospectively analyzed. Their clinical data were analyzed and the effect of the surgery was evaluated. RESULT: All patients were followed-up for more than half a year. Of all patients,12 eyes were cured, 2 eyes were improved and 0 eyes were noneffective. The total treatment effectiveness was 100%. There was no complication for all cases. CONCLUSION Nasal endoscopic dacryocystorhinostomy combined image guidance system is an effective and reliable treatment method for chronic dacryocystistis, especially for intraoperative location of lacrimal sac and control of operating process.
Dacryocystorhinostomy ; methods ; Endoscopy ; methods ; Female ; Humans ; Male ; Nasolacrimal Duct ; Nose ; Retrospective Studies ; Treatment Outcome

Peritoneal macrophages from thioglycollate-treated C57BL/6 mice were separatedby centrifugation on Percoll discontinuous gradients.Macrophages were so separ-ated into four subpopulations and their tumor cytotoxicity and the effect of sodi-um selenite on different subsets were studied.There was no marked difference inphagocytic activity and Fc recepter activity among the four subpopulations.High-density macrophages activated by MAF were kighly cytostatic and cytolyticto tumor cells,while low-density macrophages were not Peritoneal injection of sodiumSelenite(lmg/kg)augmented macrophage-mediated cytotoxic activity by increasingtheir MAF responsiveness which occurred mainly in the low-density macrophages.sodium selenite did not affect macrophage maturation as the percentage of theperoxidasepositive macrophages remained unchanged,

Objective To investigate effects of cerebral isehemic preconditioning on neurological function,in- farct volume,and the expression of caspase-3 in brain issue after brain ischemia again,and to investigate the brain protection mechanism produced by cerebral ischemia preconditioning.Methods 48 healthy male Sprague-Dawley (SD)rats(weighted 200～250g)were randomly divided into 2 groups:ischemia group(n=24),ischemia precondi- tioning group(namely preconditioning group,n=24).Each group was divided into 4 subgroups according to 6h,1d, 2d,4d after ischemia again.Results At the same point of time after ischemia,neurological deficit in preconditioning group was much less than that in ischemia group,the difference was significant(P

Objective To investigate the role of Wnt signal pathway related proteins in the tumorigenesis of sponta-neous breast cancer in TA2 mice. Methods Mammary glands were collected from tissues of normal 5 cases of TA2 mice. Spontaneous breast cancers and relevant mammary glands precancerous lesions were harvested from 5 cases of TA2 mice. Mammary tissue samples from normal TA2 mice, precancerous lesions and cancer tissues of spontaneous breast cancer-bearing TA2 mice were subsequently used to detect the expression of Wnt1,Wnt5a andβ-catenin via immunohistochemical staining. Results ① Results of immunohistochemical staining showed that the positive ex-pression of Wnt1 and Wnt5 a located in cytoplasm in spontaneous breast cancers and mammary gland precancerous lesions from TA2 mice. The normal mammary glands were negative for Wnt1 and Wnt5a staining. The cytoplasm of precancerous lesions showed higher expression for Wnt1 and Wnt5a than them in the breast cancer. β-catenin in breast cancer and precancerous lesions were abnormal cytoplasmic expression. The expression level in breast cancer was higher than in the precancerous lesions. In normal mammary gland, the positive expression ofβ-catenin mainly located in the cytomembrane. ② The positive expression rates of Wnt1, Wnt5a and β-catenin in normal breast group,precancerous group and breast cancer group were ( 4. 46 ± 3. 57 )%, ( 96. 50 ± 6. 30 )%, ( 90. 00 ± 1. 17 )%, ( 2. 27 ± 4. 55 )%, ( 96. 24 ± 6. 00 )%, ( 95. 90 ± 4. 32 )%, ( 4. 78 ± 3. 57 )%, ( 60. 59 ± 5. 63 )%, (84. 87 ± 2. 56)%,respectively. The positive expression rates of the three in precancerous group and breast cancer group were significantly higher than those in normal breast group(P<0. 01). The positive expression rates of Wnt1, Wnt5a in precancerous group were higher than those in the tumor group,and the differences of Wnt1 had statistical significance (P<0. 05) while Wnt5a did not (P>0. 05). Compared with breast cancer group,the precancerous group had lower positive expression rate of β-catenin and the differences also had statistical significance ( P <0. 05 ) . Conclusion Wnt1 , Wnt5 a and β-catenin promote the progression of spontaneous breast cancer in TA2 mice.

Objective To analyze the inputs and outputs of Health Human Resource for County -level Hos-pitals in Western China , discuss the reasons for low efficiency and quality , and provide references for improving the efficiency and quality of health human resources .Methods Using rank-sum ratio to evaluate the health human re-sources of 33 county -level hospitals of 11 provinces in western China , and to compare the evaluation results . Results In the comprehensive analysis of human input and output of health human resources between 11 provinces , according to the health human resources efficiency level of 11 provinces into third gear:a hard stop ( high-grade):Sichuan, Guangxi;second gear (mid-range): Gansu, Guizhou, Yunnan, Shaanxi, Ningxia, Inner Mongolia, Ti-bet, Xinjiang;third gear (low-grade):Qinghai.Conclusion In the nationwide perspective , the efficiency of the western provinces of health human resources is lower , and we need to take measures to improve the efficiency of health human resources .

Objective:To investigate effects of depside salt from salvia miltiorrhiza(DSSM)on the dental pulp of rats under repeated exposure to +Gz acceleration.Methods:27 male SD rats were randomly divided into 9 groups(n =3).The rats in negative control group was only fixed with rats kept-keeling device,in positive control group were exposed to +5 Gz and +10 Gz respectively for 30 s each time,5 times per day,4 days per week,for 3 consecutive weeks.The rats in other groups were respectively given DSSMat 2,6 and 18 mg/kg respectively 30 min before each +Gz exposure.The rats in negative and positive control groups were injected with 0.9%sodium chloride solution 30 min prior to +Gz exposure.After the experiment,The pulp changes were observed by HE staining.Real time qPCR was applied to determine the relative expression of heat sheck protein(HSP70)mRNA.Results:+Gz exposure decreased the activity degree of the rats,and resulted in damage of rat dental pulp.With the increase of +Gz value,the relative expression of HSP70 mRNA was increased(P ＜0.05).HSP70 mRNA expression in drug groups was less than that in their own positive control groups,but showed statistical significance only in the 18 mg/kg and +10 Gz group(P ＜0.05).Conclusion:+Gz exposure may dam-age dental pulp.DSSMhas certain protective effect under +Gz exposure.

Acupuncture Therapy ; methods ; Adult ; Craniocerebral Trauma ; complications ; therapy ; Electroacupuncture ; Female ; Hearing Loss, Sensorineural ; etiology ; therapy ; Humans ; Male ; Middle Aged ; Scalp ; Young Adult

Objective To research the quality control of determination of formaldehyde concentration in the indoor air in the newly decorated houses with 4-amino-3-hydrazine-mercapto-1, 2, 4-amilinoazaole spectrophotometry. Methods 35 newly decorated houses in two urban districts of Guiyang were chosen and the air samples were collected from March to December in 2004. Formaldehyde concentration was determined as soon as the air samples were collected, and the laboratory internal quality was controlled by using the standard sample in the determination. Results The formaldehyde concentration range of 35 newly decorated houses was 0.01-1.71 mg/m3, the detection rates was 100%, the rate of exceeding national standard was 77.1% and the median was 0.28 mg/m3. The recovery rates of quality control samples were 90.70%-103.00%. Conclusion The quality control method used in the present paper can make the results of formaldehyde concentration determinated with 4-amino-3-hydrazine-mercapto-1, 2, 4-amilinoazaole spectrophotometry be controlled in the demanded ranges of errors.

Objective:To study methods for inducing CD34+ cells of human bone marrow to differentiate into T cells in vitro to provide theory and method basics for the investigation of activity of T cells derived from psoriatic bone marrow CD34+ cells and establish a technological platform to investigate T lymphopoiesis activity of hematopoietic cells.Methods:Bone marrow CD34+ hematopoietic progenitor cells were isolated by immunomagnetic cell selection and induced differentiate into T cells in the bone in the marrow and thymic stromal microenvironment.Immunfluorescence dying method and flow cytometry analysis were performed to detect CD1-CD3+ cells,CD3+CD4+CD8-cells and CD3+CD4-CD8+ cells dynamically.Results:In the first week,the non-adhension cells were composed mostly of immature CD1+CD3-cells and CD1+CD3+ cells and small proportion of mature CD1-CD3+ cells.In the following analysis,the proportion of immature cells rapidly decreased and CD1-CD3+ cells increased.After 1 week culture,CD4+CD8+ double positive T cells and a small population CD4+CD8-and CD4-CD8+ could be detected among the CD3+ cells.In the following culture,the proportion of CD4+CD8+ double positive T cells decreased significantly and single positive T cells increased gradually.However,small proportion of mature T cells could be detected in the early stage and cann't be found after 4 weeks in the culture system without thymic stromal cells.Conclusion:Mature single positive T cells can develop from CD34+ hematopoietic progenitor cells in the bone marrow and thymic stromal microenvironment and the thymic stromal cells are vital for T lymphopoiesis.