OBJECTIVE: To estimate the value of nasal endoscopic dacryocystorhinostomy combined image guidance system in treating chronic dacryocystistis. METHOD: Thirteen cases (14 eyes) performed surgeries with nasal endoscopic dacryocystorhinostomy combined image guidance system from January 2010 to August 2013 were retrospectively analyzed. Their clinical data were analyzed and the effect of the surgery was evaluated. RESULT: All patients were followed-up for more than half a year. Of all patients,12 eyes were cured, 2 eyes were improved and 0 eyes were noneffective. The total treatment effectiveness was 100%. There was no complication for all cases. CONCLUSION Nasal endoscopic dacryocystorhinostomy combined image guidance system is an effective and reliable treatment method for chronic dacryocystistis, especially for intraoperative location of lacrimal sac and control of operating process.
Dacryocystorhinostomy ; methods ; Endoscopy ; methods ; Female ; Humans ; Male ; Nasolacrimal Duct ; Nose ; Retrospective Studies ; Treatment Outcome

Objective To investigate effects of cerebral isehemic preconditioning on neurological function,in- farct volume,and the expression of caspase-3 in brain issue after brain ischemia again,and to investigate the brain protection mechanism produced by cerebral ischemia preconditioning.Methods 48 healthy male Sprague-Dawley (SD)rats(weighted 200～250g)were randomly divided into 2 groups:ischemia group(n=24),ischemia precondi- tioning group(namely preconditioning group,n=24).Each group was divided into 4 subgroups according to 6h,1d, 2d,4d after ischemia again.Results At the same point of time after ischemia,neurological deficit in preconditioning group was much less than that in ischemia group,the difference was significant(P

Peritoneal macrophages from thioglycollate-treated C57BL/6 mice were separatedby centrifugation on Percoll discontinuous gradients.Macrophages were so separ-ated into four subpopulations and their tumor cytotoxicity and the effect of sodi-um selenite on different subsets were studied.There was no marked difference inphagocytic activity and Fc recepter activity among the four subpopulations.High-density macrophages activated by MAF were kighly cytostatic and cytolyticto tumor cells,while low-density macrophages were not Peritoneal injection of sodiumSelenite(lmg/kg)augmented macrophage-mediated cytotoxic activity by increasingtheir MAF responsiveness which occurred mainly in the low-density macrophages.sodium selenite did not affect macrophage maturation as the percentage of theperoxidasepositive macrophages remained unchanged,

Objective To analyze the inputs and outputs of Health Human Resource for County -level Hos-pitals in Western China , discuss the reasons for low efficiency and quality , and provide references for improving the efficiency and quality of health human resources .Methods Using rank-sum ratio to evaluate the health human re-sources of 33 county -level hospitals of 11 provinces in western China , and to compare the evaluation results . Results In the comprehensive analysis of human input and output of health human resources between 11 provinces , according to the health human resources efficiency level of 11 provinces into third gear:a hard stop ( high-grade):Sichuan, Guangxi;second gear (mid-range): Gansu, Guizhou, Yunnan, Shaanxi, Ningxia, Inner Mongolia, Ti-bet, Xinjiang;third gear (low-grade):Qinghai.Conclusion In the nationwide perspective , the efficiency of the western provinces of health human resources is lower , and we need to take measures to improve the efficiency of health human resources .

Objective To investigate the role of Wnt signal pathway related proteins in the tumorigenesis of sponta-neous breast cancer in TA2 mice. Methods Mammary glands were collected from tissues of normal 5 cases of TA2 mice. Spontaneous breast cancers and relevant mammary glands precancerous lesions were harvested from 5 cases of TA2 mice. Mammary tissue samples from normal TA2 mice, precancerous lesions and cancer tissues of spontaneous breast cancer-bearing TA2 mice were subsequently used to detect the expression of Wnt1,Wnt5a andβ-catenin via immunohistochemical staining. Results ① Results of immunohistochemical staining showed that the positive ex-pression of Wnt1 and Wnt5 a located in cytoplasm in spontaneous breast cancers and mammary gland precancerous lesions from TA2 mice. The normal mammary glands were negative for Wnt1 and Wnt5a staining. The cytoplasm of precancerous lesions showed higher expression for Wnt1 and Wnt5a than them in the breast cancer. β-catenin in breast cancer and precancerous lesions were abnormal cytoplasmic expression. The expression level in breast cancer was higher than in the precancerous lesions. In normal mammary gland, the positive expression ofβ-catenin mainly located in the cytomembrane. ② The positive expression rates of Wnt1, Wnt5a and β-catenin in normal breast group,precancerous group and breast cancer group were ( 4. 46 ± 3. 57 )%, ( 96. 50 ± 6. 30 )%, ( 90. 00 ± 1. 17 )%, ( 2. 27 ± 4. 55 )%, ( 96. 24 ± 6. 00 )%, ( 95. 90 ± 4. 32 )%, ( 4. 78 ± 3. 57 )%, ( 60. 59 ± 5. 63 )%, (84. 87 ± 2. 56)%,respectively. The positive expression rates of the three in precancerous group and breast cancer group were significantly higher than those in normal breast group(P<0. 01). The positive expression rates of Wnt1, Wnt5a in precancerous group were higher than those in the tumor group,and the differences of Wnt1 had statistical significance (P<0. 05) while Wnt5a did not (P>0. 05). Compared with breast cancer group,the precancerous group had lower positive expression rate of β-catenin and the differences also had statistical significance ( P <0. 05 ) . Conclusion Wnt1 , Wnt5 a and β-catenin promote the progression of spontaneous breast cancer in TA2 mice.

Objective:To investigate effects of depside salt from salvia miltiorrhiza(DSSM)on the dental pulp of rats under repeated exposure to +Gz acceleration.Methods:27 male SD rats were randomly divided into 9 groups(n =3).The rats in negative control group was only fixed with rats kept-keeling device,in positive control group were exposed to +5 Gz and +10 Gz respectively for 30 s each time,5 times per day,4 days per week,for 3 consecutive weeks.The rats in other groups were respectively given DSSMat 2,6 and 18 mg/kg respectively 30 min before each +Gz exposure.The rats in negative and positive control groups were injected with 0.9%sodium chloride solution 30 min prior to +Gz exposure.After the experiment,The pulp changes were observed by HE staining.Real time qPCR was applied to determine the relative expression of heat sheck protein(HSP70)mRNA.Results:+Gz exposure decreased the activity degree of the rats,and resulted in damage of rat dental pulp.With the increase of +Gz value,the relative expression of HSP70 mRNA was increased(P ＜0.05).HSP70 mRNA expression in drug groups was less than that in their own positive control groups,but showed statistical significance only in the 18 mg/kg and +10 Gz group(P ＜0.05).Conclusion:+Gz exposure may dam-age dental pulp.DSSMhas certain protective effect under +Gz exposure.

Mutations and deletions of the tumour suppressor PTEN are frequently involved in the development of cancer.However, PTEN is also tightly controlled by various non-genomic mechanisms,such as the epigenetic silen-cing of PTEN, post-transcriptional regulation by non-coding RNAs and post-translational modification.

Acupuncture Therapy ; methods ; Adult ; Craniocerebral Trauma ; complications ; therapy ; Electroacupuncture ; Female ; Hearing Loss, Sensorineural ; etiology ; therapy ; Humans ; Male ; Middle Aged ; Scalp ; Young Adult

Objective To evaluate the clinical efficacy of direct percutaneous coronary intervention (PCI) in ST segment elevation acute myocardial infarction (AMI) Methods From April 2000 to April 2004, 458 patients with ST segment elevation AMI were randomized to receive invasive therapy ( n =205) or non invasive therapy ( n =253) Comparison was made in the 30 day mortality rate, major cardiac events rate, reinfarction rate during hospitalization, left ventricular ejection fraction (LVEF) and end diastolic diameter (EDD) of echocardiograph in 2 weeks after AMI between the 2 groups The PCI group was divided into 3 sub groups according to number of stenosis artries found during angiography We try to find not the relation between left ventricular function and the number of coronary artery with stenosis Results The result of selective coronary artery angiography in 205 PCI patients was: 66 patients with single branch lesion (32 2%), 68 patients with double branches lesion (33 2%) and 71 patients with triple branches lesion (34 6%) 203 patients were operated successfully (99%), 228 stents were placed on lesions, 194 patients (94 6%) obtained TIMI grade Ⅲ perfusion of the IRA Compared the invasive with the non invasive group, the in hospital 30 day mortality rate was 2 9% vs 9 1% ( P

Objective To evaluate the short-term effects of three-dimensional conformal radiotherapy (3D-CRT) associated with transcatheter arterial chemoembolization (TACE) in the treatment of primary hepatic carcinoma and 3D-CRT associated with chemotherapy in the treatment of metastatic hepatic carcinoma. Methods In 52 patients with primary hepatic carcinoma, first of all a single TACE was conducted. Then after an interval of 2 weeks, the conformal radiotherapy was given, with a radiation dose of 2~3 Gy every fraction, once daily or once every other day and 3~5 fractions every week. The total radiation dose to the target was 42.2~60.0 Gy (mean, 52.2 Gy). Afterwards, 2 additional TACEs were given after the radiotherapy. In 35 patients with metastatic hepatic carcinoma, chemotherapy was carried out for 1 treatment course firstly. Then 3D-CRT was employed. After the radiotherapy, another 2 courses of consolidating chemotherapy were administrated. The patient continued with the chemotherapy for additional 3 treatment courses (a total of 6 courses) if efficacy evaluation showed the patient had responded to the chemotherapy. Results The response outcomes were: complete remission (CR) 23 0% (20/87), partial remission (PR) 39 1% (34/87), stable disease (SD) 34 5% (30/87) and progressive disease (PD) 3 4% (3/87), the response rate (RR) being 62 1% (54/87). Conclusions 3D-CRT with TACE for primary hepatic carcinoma and 3D-CRT with chemotherapy for metastatic hepatic carcinoma offers high local control rates and good short-term effects.