Objective To investigate the molecular mechanism of Klebsiella pneumoniae resistant to imipenem.Methods Anti-microbial susceptibility was evaluated by disk diffusion test,Seven imipenem-resistant strains of Klebsiella pneumoniae were select-ed.And PCR was used to amplify so as to detect eighteen resistant genes.Results Seven imipenem-resistant strains of Klebsiella pneumoniae were more sensitive to aminoglycosides and sulfamethoxazole/trimethoprim.The positive rates of KPC,ant(3′′)-Ⅰ, SHV,CTX-Mand ant(2′′)-Ⅰin these imipenem-resistant strains were high.Conclusion Carbapenem-resistant Klebsiella pneumon-iae isolated from our hospital carries several kinds of resistant genes.Carrying KPC may be the main resistant mechanism of Kleb-siella pneumoniae resistant to carbapenem,but other mechanisms also exist at the same time.

To determine the ATPase activity of erythrocytic membrane and intraerythrocyticionic concentrations in 20 patients with chronic renal failure (CRF). Methods: Erythrocyte membrane AT-Pase activites were determined as described by Sha1ev, and erythrocyte Na+ and Ca2+ concentrations weredetected by atomic absorption spectrophotometry. Results: The Na+-K+-ATPase, Ca2 t -ATPase, Mg2+ATPase activities were significantly lower in CRF patients than in normal individuals (P

Hamartoma ; congenital ; pathology ; Humans ; Mesoderm ; Mouth ; Mouth Diseases ; congenital ; pathology

OBJECTIVE:To observe the clinical efficacy and safety of recombinant human endostatin (rh-endostatin) combined with Lobaplatin for injection and irinotecan injection in the treatment of advanced recurrent small cell lung cancer (SCLC). METHODS:A total of 88 patients with advanced recurrent SCLC in our hospital were divided into control group (41 cases) and observation group (47 cases) according to random number table. Control group was given Irinotecan injection+Lobaplatin for injection. Observation group was additionally given Recombinart human endostatin injection 15 mg added into 0.9%Sodium chloride injection 250 mL,ivgtt,qd,for consecutive 14 d,every 14 d. A treatment course lasted for 28 d,and both groups were treated for 2 courses. The clinical efficacy,the levels of serum carcinoembryonic antigen(CEA),the scores physical state (ECOG) and quality of life (QOL) before,after treatment were observed in the two groups,and the survival and adverse reactions of the two groups were compared. RESULTS:The total response rate of observation group was 59.6%,which was higher than 43.9% of control group,but there was no statistical significance (P>0.05). Before treatment, there was no significant difference in serum CEA levels,ECOG scores or QOL scores,between 2 groups(P>0.05);after treatment,the serum CEA levels of the two groups were significantly decreased,and the observation group was significantly lower than the control group,with statistically significant (P<0.05). In observation group,ECOG scores decreased while QOL scores increased significantly,and significantly better than the control group,with statistical significance(P<0.05). The overall survival(OS)of observation group was 16.8 months,which was significantly higher than 11.5 months of control group,with statistical significance (P<0.05). The incidence of leucopenia in observation group was significantly higher than control group;the incidence of leucopenia and abnormal liver function were significantly lower than control group,with statistical significance(P<0.05). CONCLUSIONS:Rh-endostatin injection combined with lobaplatin and irinotecan can improve serum CEA levels and the quality of living aswell as prolong the survival time.

Objective To explore the correlation between MRI performance and biological factors of estrogen receptor (ER),progesterone receptor (PR),human epidermal growth factor receptor 2 (c-erbB-2)and p53 through a group cases of breast cancer.Methods The MRI characteristics of 39 cases with breast cancers confirmed by surgery were analyzed .The MRI characteristics of rim enhancement,speculate, the type of time-intensity curve (TIC),the early-phase enhancement ratio,peak to time,apparent diffusion coefficient (ADC)value were recorded and the lesions’pathological results of inmmunohistochemistry:the expression of ER,PR,c-erbB-2,p53 were aslo collected.The correlation between MRI characteristics and biological factors were analyzed with statistical software SPSS1 9.0.Results Spiculate was positively correlated with ER positive expression in breast cancer.Peak time of breast cancer and positive expression of p53 was negatively correlated.ADC value of breast cancer and positive expression of p53 was negatively correlated.Conclusion There are certain correlations between MRI characteristics and biological factors of breast cancer ER,PR,c-erbB-2,p53.

Objective:To study the mechanism of moxibustion therapy on diabetic peripheral neuropathy for the peripheral neuroprotection.Methods:The DPN model was induced by intraperitoneal injection with streptozotocin (STZ).The rats were given moxibustion at the acupoint Yishu (Extra) and the acupoint Zusanli (ST 36).The treatment was carried out once a day and 15 minutes per acupoint,lasting for 56 d in total.The clinical effect of moxibustion was evaluated by detecting blood sugar,urine sugar,body weight and dietary intakes,as well as nerve conduction velocity with neuroelectrophysiological method.The structure variation of sciatic nerve was observed by HE staining and light microscopy,and the level of NGF in the sciatic nerve Was determined by ELISA.Results:Compared with the model group,the plasma glucose was significantly lower in the moxibustion group (P＜0.01),with significantly faster nerve conduction velocity (P＜0.01),more notably changes in pathological appearance (P＜0.01) and higher level of nerve growth factor (NGF)(P＜0.01).Conclusion:Moxibustion could improve the symptom and signs of peripheral neuropathy in rat models with DPN,which may relate to the increased NGF and enhanced peripheral nerve protection.

Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile oils. Recently, much attention has been payed to varlous research of A. fragrans. This paper reviewed and summarized the chemical components to provide the theoretical basis for the use of Ainsliaea.
Asteraceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Molecular Structure

OBJECTIVETo evaluate the effects of Shenfu Injection (SF) on cytokines during cardiopulmonary bypass (CPB) in infants.

METHODSTwenty-four infants with congenital heart disease, aged below three years, were randomly assigned to the control group and the SF group equally. In the SF group, 1 mL/kg of SF was given through center vein pump after center vein puncture being performed, while only normal saline was given instead in the control group. Blood sample was obtained for measurement of serum necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) concentration by ELISA, at various time points in the process, i.e. right after anesthesia induction (T1), beginning of CPB (T2), aortic off-clamping (T3), 20 min after CPB (T4), the end of CPB (T5), and 6 h (T6) and 24 h (T7) after CPB.

RESULTSThe time for reverting to sinus heart rhythm and analepsia after CPB was shorter in the SF group than in the control group (P < 0.05). Serum concentration of TNF-a and IL-6 was equal in the two groups at T1; they increased significantly after CPB (P < 0.05), reached the peak value at T4 and reduced to the normal level at T7 in the control group. TNF-alpha concentration was significantly lower at T3, T4 and T5, and IL-6 concentration was lower at T4 in the SF group than that in the control group at corresponding time point (P < 0.05).

CONCLUSIONSF could shorten the time for reverting to sinus heart rhythm and analepsia after CPB in infants, and suppress the inflammatory response caused by CPB.

Cardiopulmonary Bypass ; Child, Preschool ; Cytokines ; blood ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Heart Defects, Congenital ; blood ; drug therapy ; surgery ; Humans ; Infant ; Injections, Intravenous ; Interleukin-6 ; blood ; Male ; Phytotherapy ; Tumor Necrosis Factor-alpha ; blood

Objective To investigate the relationship between the endoscopic characteristics of chronic gastritis,duodenitis,peptic ulcer and their histopathologic findings in children,and explore the relationship between Helicobacter pylori(Hp) infection and the severity of histopathologic changes of gastroduodenal mucosa in children. Methods Three hundreds and sixty-six children with chronic upper gastrointestinal symptoms who were examined by gastroscopy were enrolled.The gastric and duodenal mucosal biopsy specimens of all the patients were studied histopathologically. ResultsTypes of chronic gastroduodenal diseases in all these patients were: chronic gastritis(n=206,56.3%),chronic gastritis combined with duodenitis(n=112,30.6%),chronic gastritis combined with peptic ulcer(n=48,13.1%).There was chronic inflammation in gastric mucosa and duodenal bulb mucosa in all the cases examined by histopathologic examination.Hp infection was found in 106 cases(28.96%).The gastric mucosal inflammation was more severe in those with Hp infection than those without(P0.05).The were significant differences in the incidences of inflammation activity,atrophia and nodulus lymphaticus of gastric mucosa between those with Hp infection and those without(P

AIM: To evaluate the efficacy of combined therapy for amblyopia in children by making use of pattern visual evoked potential ( P-VEP) game.
METHODS: This was a prospective case control study. These asthenopic children were divided into two groups. The control group ( 66 eyes of 49 patients ): occlusive therapy with glasses, cover, precision work, red light treatment and so on, later the stereo vision training was added. The experimental group (72 eyes of 52 patients):conventional methods mentioned above with P - VEP games.
RESULTS: The total effective rate and cure rate of experimental group in 6mo were higher than those of control group. The overall effective rate was 94. 4% in the experimental group and 83. 3% in the control group. There was a statistically significant difference between them (P<0. 05).
CONCLUSION: The comprehensive therapy by making use of P-VEP game is an individualized effective new way in treating amblyopia.