AIM: To study the effects of L-arginine on sebaceous gland hyperplasia in rats. METHODS: 10 mg?kg -1?d -11 of L-arginine was poured into the stomach in Sprague-Dawley rats for 7-14 d. The pathology phenomenon was observed in the rat skin. RESULTS: The backside skin of the male SD rats showed dewiness, greasiness and engrained yellow. The incidence was 100% for 7 d in the male SD rats, 80% in the female SD rats for 14 d, but 100% in the immature female SD rats for 14 d. CONCLUSION: L-arg nine can facilitate the hyperplasia of sebaceous gland in SD rats.

OBJECTIVETo observe morphological changes of enteric nervous system (ENS)-interstitial cells of Cajal (ICC)-smooth muscle cell (SMC) structure injury in deep muscle nerve plexus offunctional dyspepsia (FD) rats, and the repair of Shuwei Decoction (SD) on it, and to explore its effecton FD.

METHODSTotally 72 rats were randomly divided into the control group, the model group, the lowdose SD group, the medium dose SD group, and the high dose SD group, the Mosapride group, 12 ineach group. Rats in the low dose SD group, the medium dose SD group, and the high dose SD group were intragastrically fed with SD at 0.767, 1.534, 3.068 g/mL, respectively. Rats in the Mosapride group were intragastrically fed with Mosapride (1.37 mg/kg). FD rat model with Gan depression Pi deficiency syndrome (GDPDS) was established using complex pathogenic factors. Corresponding liquors were respectively administered to rats in corresponding groups from the 3rd day after modeling. Distilled water(10 mL/kg) was administered to rats in the control group and the model group, once per day for 14 successive days. Rats were sacrificed and small intestine tissues collected for observing ENS-ICC-SMC structure injury using immunofluorescence double labeling, laser scanning confocal microscope, and transmission electron microscope at day 15. Repair of SD on it was also observed.

RESULTSENS-ICC SMC structure was incomplete, with obvious injury in mutual link of ICC, ICC, SMC, and connecting structure. ENS-ICC-SMC structure was more complete in high, medium, and low dose SD groups, with close link of ICC and SMO. Their connecting structures were in good conditions.

CONCLUSIONSD could keep the integrity of ENS-ICC-SMC structure by promoting regeneration and morphology of ICC, thereby, improving gastrointestinal movement disorder and showing therapeutic effect on FD.

Animals ; Benzamides ; pharmacology ; Drugs, Chinese Herbal ; pharmacology ; Dyspepsia ; drug therapy ; Enteric Nervous System ; drug effects ; Interstitial Cells of Cajal ; drug effects ; Morpholines ; pharmacology ; Muscle, Smooth ; drug effects ; Random Allocation ; Rats

Objective To detect the doubling time of airway smooth muscle cells of human and rat by the xCELLigence in-strument,a real time cellular analyzer.Methods The airway smooth muscle cells were separated by collagenase-pancreatin digestion from the rat airway.Then,added to the different holes of E-plate of xCELLigence instrument with the planting destiny of 3 000 cells/well.and so were the human airway smooth muscle cells.The E-plate was then placed on the xCELLi-gence instrument to monitor cell proliferation for 100 hours to calculate the doubling time by using the RTCA Software Package 2.0 software.Results The doubling time of human airway smooth muscle cell calculated by the real time cellular analyzer was 23.96±0.47 h,which was consistent with the data provided by the reference.The doubling time of rat airway smooth muscle cell was 18.62±0.15 h,and the computational process was simple,time-saving and also effective.Conclusion The xCELLigence instrument can be used to calculate doubling time of airway smooth muscle cells of human and rat, which provides experimental methods and reference data for the basic respiratory disease research.

Objective To compare the accuracy of BIS and skin conductance (SC) for assessment of the depth of sedation induced by target-controlled infusion (TCI) of propofol. Methods Thirty ASA Ⅰ orⅡpatients aged 21-56 yr weighing 52-85 kg undergoing orthopedic operation on the lower limb under epidural anesthesia were enrolled in this study.After the onset of epidural anesthesia, TCI of propofol was started at an initial target plasma concentration (Cp) of 0.8 μg/ml. Th Cp was increased by 0.5-0.8 μg/ml every 3 min until OAA/S score=1. OAA/S score, BIS and SC values were recorded, SC change value (△SC) was calculated. Spearman rank-order correlation, receiver operating characteristic curve (area under curve AUC was calculated) and logistic regression were used to analyze the relationship of OAA/S score to BIS and △SC.Results BIS and △SC were significantly correlated with OAA/S scores (r were 0.920 and-0.859 respectively). The AUC of △SC (0.919, 0.946) was significantly better correlated with OAA/S score (5→4, 4→3) than that of BIS (0.761, 0.507), while BIS was better correlated (0.781, 0.959) with OAA/S score (3→2, 2→1) than ASC (0.577, 0.630). Logistic regression correctly predicted loss of consciousness. The accuracy of prediction was 93% for BIS and 82% for △SC. Conclusion The accuracy of SC for assessment of the depth of sedation induced by propofol TCI ishigher than that of BIS before loss of consciousness,while lower than that of BIS after loss of consciousness. BIS is more accurate in monitoring the loss of consciousness.

In this communication, the values of serum CA-50 in a series of patients with benign and malignant GI diseases are reported. It was found that CA-50 was positive in 86.7% of 30 pancreatic cancer patients with an average level of 427.53U/ml, which was statistically different from that found in non-malignant control group. In this series serum CA-50 determination helped to confirm the diagnosis of pancreatic cancer in 6 cases, in which B-mode ultrasono-graphy failed to make a definite diagnosis, and in another 3 cases CT scanning was unable to disclose the tumor. The combination of CA-50, B-mode ultrasonography, and CT made definite diagnosis in 100% of all 30 pancreatic cancer patients. The author discussed the significance of CA-50 determination in the diagnosis and differential diagnosis of pancreatic cancer.

Multi-component Chinese medicine has been considered an important developmental direction for traditional Chinese medicine (TCM) formula.Nowadays,it is unfit to target a molecule for preventing or curing a disease because it resulted from multiple of factors and resulted in many indications.Thus,the idea focusing on the multiple targets for therapy is increasingly accepted by physicians and scientists.Here,we conceived a new simplified method for screening the active multi-component Chinese medicine based on the complexity of Chinese medicine,the multi-target property of protein signal transduction and the principle of Chinese medicine prescription.Combined with the traditional knowledge on tumor and latest antitumor research results of Chinese medicine and its compounds,the method was concretely illustrated.It helps us to transform the herbal compounds to new complex drugs targeting multiple signaling.

Adult ; Deferoxamine ; adverse effects ; therapeutic use ; Humans ; Lung Diseases, Interstitial ; chemically induced ; Male ; Myelodysplastic Syndromes ; drug therapy

Adult ; Female ; Humans ; Leukemia ; complications ; microbiology ; Liver ; microbiology ; Male ; Mycoses ; complications ; Spleen ; microbiology ; Young Adult

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cyclophosphamide ; therapeutic use ; DNA-Binding Proteins ; metabolism ; Doxorubicin ; therapeutic use ; Female ; Germinal Center ; pathology ; Humans ; Immunohistochemistry ; Interferon Regulatory Factors ; metabolism ; Lymphoma, Large B-Cell, Diffuse ; classification ; drug therapy ; metabolism ; pathology ; Male ; Middle Aged ; Neprilysin ; metabolism ; Prednisone ; therapeutic use ; Prognosis ; Proto-Oncogene Proteins c-bcl-6 ; Vincristine ; therapeutic use

Objective To study the possible influencing factors in the formation of coronary collateral circulation in patients with chronic total occlusion (CTO). Methods Patients were enrolled having at least 1 major coronary artery angiography revealed as CTO of 144 patients. According to the Rentrop classification, patients with grade 0 and grade 1 filling were catogorized as insufficient collateral circulation group (n=72) and patients with grade 2 and grade 3 filling as collateral circulation group (n=72). Serum biomarkers and insulin-resistance by HOMA model were also studied in all patients. Results In the insufficient collateral circulation, BMI,TC,ApoB, lipoprotein a, fasting insulin HOMA-IR,HOMA- beta, CRP was significantly higher than the well collateral circulation group and ApoA-Ⅰ, ISI lower than the well collateral group ( all P ﹤0. 05 ) . Bivariate correlation alaysis showed. Rentrop score, BMI, TC, ApoB, lipoprotein a, fasting insulin, HOMA-IR,HOMA- beta and CRP are positively correlated to the formation of collateral circulation ( P ﹤ 0. 05 ); ApoA-Ⅰ and ISI were negatively correlated ( P ﹤0. 05 ) . Logistic regression analysis after calibration with weight, ApoA-Ⅰ and HOMA-beta factors, lipoprotein a ( OR 7. 575,P=0. 009), TC (OR 2. 154,P =0. 001) were found to be the independent factors of coronary collateral circulation formation. Conclusions TC, lipoprotein a, obesity, CRP, and HOMA-IR are correlated with the formation of coronary collateral circulation and may predict formation of collateral circulation in patients with CTO.