Objective To investigate the changes in the expression of hepatic circadian clock gene in different types of circadian rhythm and the effect of isoflurane anesthesia on the expression of hepatic circadian clwk gene in mice.Methods Seventy-two male C57/B6 mice,aged 2 months,weighing 20-25 g,were randomly divided into 3 groups (n =24 each):normal light/dark (LD) cycle group,reversal LD cycle group and anesthesia group.Normal LD cycle group and anesthesia groupwere maintained in a regular 12 h LD cycle with lights on at 8:00 am and off at 8:00 pm for 3 weeks,and in addition anesthesia was then performed with isoflurane in anesthesia group.Reversal LD cycle group was kept in an inverted12 h LD cycle with lights on at 8:00 pm and off at 8:00 am for 3 weeks.The natural time was converted to circadian time (CT) and the initial time was set at CT0.Isoflurane anesthesia group was exposed to 2％ isoflurane for 6 h during the wakening period from CT14 to CT20.The liver and suprachiasmatic nucleus (SCN) were removed from mice at CT2,CT8,CT14 and CT20 for determination of Clock and Cry1 mRNA expression by real-time quantitative PCR.Results Clock and Cry1 mRNA expression in the liver and SCN showed rhythm in the two different types of circadian rhythm.Compared with that in SCN,the peak phase of Clock and Cry1 mRNA expression in livers was delayed in two different types of circadian rhythm.Isoflurane anesthesia caused a peak phase delay of Cry1 and Clock mRNA expression in livers as compared with normal LD cycle group.Conclusion Circadian clock gene in livers shows rhythmic expression in different types of circadian rhythm,and isoflurane anesthesia can cause a large peak phase delay of circadian clock gene expression in livers of mice.

Objective To investigate spatial memory ability of intraperitoneal injection of resveratrol in a mice model of chronic constriction injury of sciatic nerve(CCI).Methods Forty-four C57BL/6 mice were divided randomly into 4 groups:sham group (n=14),CCI group (n=14),resveratrol pre-treatment group (i.p.resveratrol 100 mg/kg 30 minutes before CCI model,n=8) and resveratrol post-treatment group (i.p.resveratrol 100 mg/kg 14 days after CCI model,n =8).CCI group,resveratrol pre-treatment group and resveratrol post-treatment group were operated with the model of neuropathic pain induced by chronic constriction injury of sciatic nerve.In shamoperated controls,an identical surgical procedure was performed,except that the sciatic nerve was not ligated.This was accomplished by using intellicage for mice by newbehavior to record their spatial memory after surgery.Results (l) Resveratrol pre-treatment group showed improved spatial memory ability compared with sham group and CCI group during day 17-21 (17 d:(55.80±7.66) ％,(51.20±7.94) ％ ; 18 d:(60.20±3.89) ％,(49.80±8.61) ％ ; 19 d:(62.20±7.25) ％,(51.20±6.83) ％ ;20 d:(63.00±9.69) ％,(48.40±8.84) ％ ;21 d:(56.80±7.52) ％,(47.20±4.54) ％)(P＜0.05),compared with CCI group.(2)From day 26,the spatial memory damage was observed in mice with CCI (26 d:(37.50±5.50)％,(51.80±9.01)％;27 d:(37.25±4.19)％,(51.20±5.76)％;28 d:(42.25± 3.50) ％,(52.80± 7.52) ％) (P＜ 0.05),compared with sham group.And this damage could be reversed by resveratrol,which was injected when the chronic pain was stable (26 d (46.60± 5.27) ％,27 d (54.00± 7.31) ％,28 d (52.60±4.39)％),compared with CCI group(P＜0.05).Conclusion Chronic constriction injury of sciatic nerve mice due to spatial memory impairment can be improved by resveratrol.

Food processing and cooking is very practical, and requires students to master certain knowledge and operational skills of nutrition. Therefore, we introduced the experience teaching design, con-crete experiment, reflective observation, abstract generalization and application, focused on practice-appli-cation-further practice in the teaching process, readjusted and set practical objectives, contents, teaching methods, evaluation, and used questionnaire and interview method to evaluate the teaching effect, which enriched the teaching content of the course and improved the effectiveness of students'!practice learning.

The new intein-mediated PHB purify protein system is a high expression, automatic cutting, for purification, low-cost protein purification system,it is conducive to large-scale protein purification.Choose human antibacterial peptide LL-37 as the purification objects,which is poison to prokaryotic cell.We construct intein-mediated PHB purified human antimicrobial peptide LL-37 system through genetic engineering technology and use this system to purify LL-37. The results show that this system can highly express LL-37 fusion protein and purifiy the product as same size with expectations.

Adult ; Aldosterone ; blood ; Angiotensin II ; blood ; Diet, Sodium-Restricted ; Female ; Humans ; Kidney ; physiopathology ; Kidney Function Tests ; Liver Cirrhosis ; blood ; physiopathology ; Male ; Middle Aged ; Renin ; blood ; Sodium ; administration & dosage

Objective: To explore the predictors in patients of paroxysmal atrial fibrillation (AF) recurrence after radiofrequency catheter ablation (RFCA). Methods: A total of 142 PAF patients received RFCA in our hospital from 2013-03 to 2016-03 were studied. The patients were divided into 2 groups: Recurrence group, n=46 and Non-recurrence group, n=96. Clinical data was compared between 2 groups and AF recurrent predictors were studied by single and multivariate Logistic regression analysis. Based on quartiles of uric acid (UA) level, the patients were categorized in another set of 4 groups: Q1 group, UA<259 μmol/L, n=33, Q2 group, UA 259-320 μmol/L, n=37, Q3 group, UA 321-380 μmol/L, n=37 and Q4 group, UA>380 μmol/L, n=35. The influence of UA on AF recurrence was measured by Kaplan-Meier test, the predictive value of UA combining metabolic syndrome (UA+MS) on AF recurrence was studied by ROC curve analysis. Results: The BMI, diabetes, MS, AF duration, CHADS2 score, creatinine, UA and BNP were different between Recurrence group and Non-recurrence group, all P<0.05. Logistic regression analysis indicated that AF duration (OR=1.02,95% CI 1.01-1.03, P=0.002), UA level (OR=1.01, 95% CI 1.00-1.01, P=0.046) and MS (OR=4.73, 95% CI 1.36-16.45, P=0.014) were the independent predictors for AF recurrence. UA quartile analysis indicated that gender, BMI, MS, creatinine, LVEF and the incidence of AF recurrence had signifcant discrepancy by different UA levels, all P<0.05. ROC curve showed that the predictive values for UA+MS in AF recurrence had the sensitivity at 80.4%, specificity at 74.1% (AUC 0.79±0.04, 95% CI 0.71-0.89, P=0.0001), for UA in AF recurrence had the sensitivity at 73.9%, specificity at 57.2% (AUC 0.66, 95% CI 0.56-0.76, P=0.02); UA+MS had the higher predictive value than UA alone, P<0.05. Conclusion: Both UA and MS were related to AF recurrence, high UA level combining MS had certain predictive value for AF recurrence in PAF patients after RFCA.

Objective To evaluate the effects of GLYX-13 on cognitive function after long-time isoflurane anesthesia in mice. Methods A total of 192 healthy male C57∕B6J mice, aged 8 weeks, weig-hing 22-25 g, were divided into 4 groups(n=48 each)using a random number table: control group (group C), isoflurane anesthesia group(group I), GLYX-13 group(group G), and isoflurane anesthesia plus GLYX-13 group(group IG). The animals were exposed to 15% isoflurane for 6 h in I and IG groups. GLYX-13 1 mg∕kg was injected via the caudal vein at 2 h before anesthesia in G and IG groups. Novel ob-ject recognition test and contextual fear conditioning test were performed on 1st, 3rd and 7th days after an-esthesia. The expression of 2B subunits-containing NMDA receptor(NR2B)and cyclic adenosine mono-phosphate response element-binding protein(CREB)mRNA in the hippocampus was detected by quantita-tive real-time polymerase chain reaction after the end of behavioral tests on 1st, 3rd and 7th days after anes-thesia. Results Compared with group C, the percentage of time spent in exploring a novel object, dis-crimination index and percentage of freezing time were significantly decreased, and the expression of NR2B and CREB mRNA in the hippocampus was down-regulated in group I(P <005). Compared with group I, the percentage of time spent in exploring a novel object, discrimination index and percentage of freezing time were significantly increased, and the expression of NR2B and CREB mRNA in the hippocampus was up-regulated in group IG(P <005). Conclusion GLYX-13 can significantly improve the cognitive func-tion after long-time isoflurane anesthesia in mice.

Objective To investigate the relationship between hepatitis B virus (HBV)deoxyribonucleic acid (DNA) and HBV covalently closed circular DNA (cccDNA) in the ovary and HBV intrauterine infection.Methods HBV DNA and HBV cccDNA were assayed in the ovaries of 33 pregnant women who were positive for HBV DNA,tested by Fluorescent quantitative polymerase chain reaction (FQ-PCR).The level of HBV mark (HBVM) and the content of HBV DNA in peripheral blood of infants were measured by chemoluminescence and FQ-PCR methods respectively.Results The overall positive rate for both HBV DNA and HBV cccDNA in ovarian samples was 51.52％ (17/33).The rate on intrauterine infection among infants was 12.12％ (4/33) and all the 4 infected infants were delivered from mothers with normal hepatic function.When HBV DNA and HBV cccDNA were both positive,the rate of intrauterine infection in infants was significantly higher than those who were with both negative results (P＜0.05).Levels of HBV cccDNA and the rate of positive samples were significantly higher in mothers with infants who appeared to have had intrauterine infection than those did not (P＜0.01 and ＜0.05,respectively).Conclusion HBV infection could be discovered in the human ovary and might be transmitted to the filial generation via ovum.

OBJECTIVEThe incidence of hypertension in Tibet ranks highest among all Chinese provinces. This may be due to genetic changes caused by Tibet's unique natural environment and agrarian lifestyle, prompting us to investigated the relationship between gene polymorphisms and hypertension.

METHODSBlood samples were collected from 229 hypertensive participants and 372 healthy (control) participants from five Tibetan counties. Seventeen single nucleotide polymorphisms were investigated for their connection to hypertension.

RESULTSThe C allele at rs2070744 of the NOS3 gene was shown to be significantly associated with hypertension (P=0.0443; OR=1.636). Additionally, the T allele of rs4961 of the ADD gene was correlated with hypertension in women (P=0.03124; OR=1.584).

CONCLUSIONIn this study we found that the NOS3 and ADD genes were related to a high incidence of hypertension among Tibetans. NOS3 gene plays a role in regulating vascular tone and blood vessel diameter, which may be altered by the low-oxygen environment of Tibet. ADD is involved in water and salt metabolism, which is consistent with the high-salt diet of Tibetans. The correlations elucidated by our study were different from those of other ethnic groups, indicating that these findings may be specific to the Tibetan people.

Adult ; Animals ; Asian Continental Ancestry Group ; genetics ; Female ; Genotype ; Humans ; Hypertension ; genetics ; Male ; Middle Aged ; Polymorphism, Genetic ; Tibet

OBJECTIVETo evaluate the feasibility and efficacy of intraperitoneal chemotherapy for malignant ascites caused by different types of abdominal cancers guided by chemo-sensitivity methyl tetrojolium coloremetric (MTT) assay in vitro.

METHODSCancer cells in the malignant ascites were collected for MTT assay to determine the chemo-sensitivity. The drug producing the highest or the second highest inhibition rate was selected for intraperitoneal chemotherapy. The correlation between the results of MTT assay and the response of malignant ascites, the clinical features, Karnofsky performance score (KPS) and prognosis were analyzed.

RESULTSMTT assay indicated that Taxotere (TXT) and Hydroxycamptothecin (HCPT) were the most effective to cancer cells in malignant ascites, and HCPT was mostly frequently used for intraperitoneal chemotherapy (56.9%). Twenty-four patients showed response by intraperitoneal chemotherapy (complete response: 7; partial response: 17) with a slightly significant correlation between the results of MTT assay and response of malignant ascites (P = 0. 014). The KPS of the responders was improved significantly (P < 0.001), and the response of malignant ascites to intraperitoneal chemotherapy was demostrated as an independent prognostic factor by multi-variate analysis in this series.

CONCLUSIONIn vitro chemo-sensitivity MTT assay guided intraperitoneal chemotherapy for malignant ascites is simple, effective and safe, which can improve the KPS and prognosis of the responders.

Adenocarcinoma ; drug therapy ; pathology ; Adult ; Aged ; Antineoplastic Agents ; administration & dosage ; pharmacology ; therapeutic use ; Antineoplastic Agents, Phytogenic ; administration & dosage ; pharmacology ; therapeutic use ; Ascites ; drug therapy ; pathology ; Camptothecin ; administration & dosage ; analogs & derivatives ; pharmacology ; therapeutic use ; Cell Survival ; drug effects ; Colorectal Neoplasms ; drug therapy ; pathology ; Female ; Humans ; Injections, Intraperitoneal ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Pancreatic Neoplasms ; drug therapy ; pathology ; Stomach Neoplasms ; drug therapy ; pathology ; Taxoids ; administration & dosage ; pharmacology ; therapeutic use ; Tumor Cells, Cultured