Objective To investigate the changes in non-linear dynamics properties of EEG and the application of real-time monitoring of non-linear indexes during the course of general anesthesia. Methods EEG was recorded in 65 patients undergoing operations. They were randomly divided into five groups: isoflurane, sevoflurane, desflurane (n=15, respectively), propofol 8mg/(kg?h) and propofol 10mg/(kg?h) (n=10, respectively). The EEG derived parameters correlation dimension (D 2 ) and approximate entropy (ApEn) non-linear indexes were calculated simultaneously during the whole operation, including the time of entering the operation room, anesthetic induction, intraoperation, recovery, and regaining consciousness. Results At the time of entering the operation room, their D 2 and ApEn nonlinear indexes appeared to be highest. Both the indexes decreased swiftly during anesthetic induction, and they dropped to lower values and leveled off during the intra-operation period. During recovery period, both of them rose gradually and returned to a high level in the post-operation awaking period. Conclusions Changes in the depth of anesthesia could be real-timely monitored and precisely measured with non-linear indexes of EEG. Non-linear dynamic analysis might provide us with more information on consciousness during general anesthesia

0 05) and neither implicit nor explicit memory in other groups. D 2 and ApEn nonlinear indexes were higher while entering the operative room. Both indexes dropped to a relative lower value and leveled off in the intra operative period. In 5mg group, both indexes increased significantly during the memory test compared with intraoperative period (D 2 and ApEn nonlinear index were 4 07 and 3 30, 0 67 and 0 53 respectively, P

Objective To study the clinical and imaging features of paraneoplastic limbic encephalitis (PLE). Methods The clinical, latoratorial and imaging information of two patients with clinically diagnosed PLE associated with invasive thymoma or lung cancer are reported. Results Two cases had typical findings in clinical symptoms ( severe neuropsychological deficits, disorientation, memory impairment and seizures) and MRI showed ( abnormal high-signal intensity in hippocampal formations and temporal lobe on T2-weighted imagings) , One patient was investigated by cerebral 18 F-fluoro-2-deoxy-D- glucose- positron emission tomography (FDG-PET) , and highly significant focal hypermetabolism in bilateral temporal and other areas was found. Conclusion The clinical and imaging examination are the improtant method for the diagnosis of PLE.

To investigate the change in GMP 140 and its significance in patients with diabetes accompanied with cerebral infarction. Plasma GMP 140 levels were assayed by radilimmunoassay in diabetes accompanied with cerebral infarction, controlled diabetes, cerebral infarction patients and normal controls, respectively. The results showed the GMP 140 levels were significantly higher in patients with diabetes accompanied with cerebral infarction than in patients with diabetes and normal control ( P 0 05). The results suggested that the level of GMP 140 in plasma might be used as a sensitive and reliable indicator reflecting the degree of platelet activation.

ObjectiveTo investigate the therapeutic effectiveness of defibrase in treating penumbra and reperfusion.MethodsIntraluminal suture method was used to develope reversible middle cerebral artery occlusion(MCAO). Rats were subjected to MCAO 3 hours followed by reperfusion for 3, 6, 24, 72 hours, and to MCAO 6 hours followed by reperfusion for 3, 6, 24 hours. The treatment groups rats were injected intravenously defibrase at 0.5 hour before reperfusion. Meanwhile, the control group received normal saline. Clinically Neurological Deficits Scale were evaluated every day. Infarction volume was measured by using 2,3,5-triphenyltetrazolium chloride staining. Pathologic change were examined microscopically in HE stained sections.ResultsThere were significant difference at treatment groups of reperfusion 3 hours after MCAO.Infarction volume and Clinically Neurological Deficits Scale was significant reduced as control group (P<0.05). There were no significant differences at treatment groups of reperfusion 6 hours after MCAO (P>0.05). Cerebral hemorrhage wasn't increased in defibrase treatment group.ConclusionsDefibrase was effective on Clinically Neurological Deficits of rats in reperfusion 3 hours after MCAO.

Objective To explore the influence of different reconstruction modes with time?weighted respiratory phases on the internal tumor volume ( ITV) of solitary pulmonary lesion ( SPL) , and to evaluate the feasibilities of 8 and 4 equal time?weighted respiratory phases in 4DCT simulation. Methods 24 patients with SPL underwent 4D scanning. Images were reconstructed with 10, 8 and 4 equal time?weighted phases of the respiratory cycles, respectively. Gross tumor volumes ( GTVs ) were delineated on the three sets of reconstructed images and fused into ITVs, which were ITV10 , ITV8 and ITV4 respectively. The differences of volumes, centroid of the ITVs and motions of GTV centroids in three?dimensional directions were compared. Statistical analysis was performed using the Friedman M test. Results The volumes of ITV10 , ITV8 and ITV4 were (9.09±12?29) cm3,(9.10±12?47) cm3 and (8.98±12?61) cm3(P=0?001), respectively. There were no differences between the volumes of ITV10 and ITV8 after the Bonferroni correction ( P=0?721) , while the opposite between those of ITV10 and ITV4 ( P=0?002 ) . The differences of centroid positions of ITV10, ITV8 and ITV4 in x?, y?and z?axes were all less than 1 mm ((12.22±7?71),(12.23± 7?71),(12.22±7?71),Px =0?668);(43.30±29?38),(43.30±29?40),(43.31±29?39),Py =0?643;(5.66±3?67),(5.66±3?67),(5.66±3?67),Pz=0?878), similar to the motions of GTV centroids in three reconstructed modes ((0.69±0?56),(0.69±0?68),(0.79±0?51) mm,Px=0?356;(3.13±3?78),(3.13± 4?05),(3.19±4?06) mm,Py =0?978;(1.18±1?31),(1.03±1?32),(1.16±1?34) mm,Pz=0?302). Conclusions There were no differences in volumes, centroid positions and motions between ITV10 and ITV8 . The quantity of reconstruction images and GTV delineations according to 8 time?weighted phases were both less than conventional 10 phases. 8 time?weighted respiratory phases mode was feasible in 4DCT simulation for SPL.

Objective To construct the genes expression profile database of experimental autoimmune encephalomyelitis (EAE) mice model and to screen the high candidate genes with microarray analysis. Methods Polymerase chain reaction (PCR) products on behalf of 4 096 genes from mixed mice tissue library were assembled on the modified Oako glass slide. The general RNA from 6 EAE mice model and 6 normal mice head was transcripted into cDNA by RT-PCR and labelled with cy-3 and cy-5.6 groups each consist one EAE mice and a control. These two probes were mixed and hybridized with the above mentioned gene chips. Scan array 4000 was used for scanning the hybridizing signals and GenePixPro 3.0 for date analysis. Results Of the total 4 096 genes monitored, 43 genes in group one showed changes in expression level of the ratio outside 0.5 and 2, 30 genes differed in group two, 176 genes changed in group three, 76 in group four, 294 in group five and 129 in group six. Conclusions The results showed the consistent different genes expression throughout the EAE mice model. The genes related to immune, cell structure, cell cycle, ion channel, signal transduction, protein synthesis and metabolism are involved in EAE pathogenesis. The results provide deeper insights into the mechanism of EAE and multiple sclerosis.

ObjectiveTo investigate the changes of non-linear dynamics characteristics of EEG of the patients with menopause depression under eyes closed state.MethodsEEG were recorded in 18 patients with menopause depression and 18 healthy volunteers under eyes closed state. Approximate entropy (ApEn) was calculated for all subjects.ResultsApEn of the menopause depression group at eyes closed state increased significantly compared with that of the healthy control group.ConclusionDynamic non-linear analysis is more appropriate for the study of mental functions changes of menopause depression.

ObjectiveTo investigate the changes of non-linear dynamics characteristics of EEG during picture memory masks, and the application of non-linear dynamic analysis for memory research.MethodsEEGs of 30 healthy old volunteers were recorded under the states of opened eyes and picture memory. Correlation dimension (D2) was calculated for all subjects.ResultsD2 increased significantly during memory tasks compared with the rest state with eyes opened (P<0.05). In different memory tasks, the complexity of mental functions and the regions involved in the memory task were different.ConclusionNon-linear analysis is appropriate for the study of functional changes and working mechanism of brain during memory.