Objective: To study the relation of condylar morphology with different vertical facial types in the development of patients with skeletal Ⅲ malocclusions. Methods:180 cases with skeletal Ⅲ malocclusion were divided into children(5-11 years old), adolescence(12-17 years old) and adult(18-30 years old) groups with 60 cases in each group. Orthopantomograms of the patients with different vertical facial types were retrospectively investigated by computerized cephalometric analysis. Condylar morphology were compared among different age groups of the same vertical facial type. Results:In patients with high angle, ramus height(RH) was getting bigger with ageing(P0.05). In patients with low angle h and RH in adult group were bigger than those in adolescence or in children(P

OBJECTIVETo study the difference of condylar morphology with different vertical facial types of skeletal III malocclusions in mixed dentition.

METHODSOrthopantomograms of 60 children with different vertical facial types were retrospectively investigated by computerized cephalometric analysis. Condylar morphology was compared among different vertical facial type groups.

RESULTSThere was statistical difference in upper condylar height among groups, the height increased from high angle group, average angle group to low angle group. There was no significant difference in ramus height in the groups. There was statistical difference in the upper condylar height to the ramus height radio in different vertical facial type groups (P < 0.01). The ratio increased from high angle group, average angle group to low angle group. There was no significant difference in the condylar height to the condylar neck width ratio in the groups.

CONCLUSIONIn children of skeletal III malocclusions in mixed dentition with different vertical facial types, there are statistical differences in condylar morphology, which might be due to different growth of mandibular.

Cephalometry ; Child ; Dentition, Mixed ; Face ; Female ; Humans ; Male ; Malocclusion ; Mandible ; Radiography, Panoramic ; Retrospective Studies

OBJECTIVEThe present study is concerning qualitative and quantitative detection of Poria cocos quality based on FT-near infrared (FT-NIR) spectroscopy combined with chemometrics.

METHODThe Poria cocos polysaccharides contents were determined by UV. Transmission mode was used in the collection of NIR spectral samples. The pretreatment method was first derivation and vector normalization. Then principal component analysis (PCA) was used to build classification model and partial least square (PLS) to build the calibration model.

RESULTThe results showed that conventional criteria such as the R, root mean square error of calibration (RMSEC), and the root mean square error of prediction (RMSEP) are 0.944 0, 0.072 1 and 0.076 2, respectively. The misclassified sample is 0 using the qualitative model built by PCA.

CONCLUSIONThe prediction models based on NIR have a better performance with high precision, good stability and adaptability and can be used to predict the polysaccharose content of Poria cocos rapidly, which can provide a fast approach to discriminate the different parts of Poria cocos.

Fungal Polysaccharides ; analysis ; Least-Squares Analysis ; Poria ; chemistry ; Principal Component Analysis ; Spectroscopy, Near-Infrared ; methods

By means of genetic cloning and recombinant techniques, full genome cDNA sequences of rotavirus strain TB-Chen were isolated from an infantile hospitalized with acute gastroenteritis. Nucleotide sequences analyses showed that the full genome of strain TB-Chen contains 18613 nucleotides, encoding 5791 amino acids. Genotyping results showed that the strain TB-Chen belongs to genotype G2P[4]/NSp4[A]. This is the first report on a full genome of Group A rotavirus in China, and has important significance for deep understanding structure and functions of rotaviruses and developing rotavirus vaccines.

This study was purposed to establish a new quick and simple diagnostic method with high sensitivity and good specificity for idiopathic thrombocytopenic purpura (ITP) and to evaluate its significance. 240 platelet lysates (from patients with ITP, leukemia, MDS, and healthy adults, each of 60 cases) were randomly assigned to training set (120 cases) or validation set (120 cases), all of them were detected by surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS), in order to identify the differentially expressed protein, the diagnostic model was established by means of artificial neural network (ANN), and was validated by blind test with SPSS 17.0. The results showed that 5 marked proteins significantly differentially expressed (P < 0.01), m/z of highly expressed proteins were 2234.30, 3476.36, and 7526.29, m/z of low expressed proteins were 4990.02 and 5152.39, respectively. The sensitivity and specificity of diagnostic model were 80.6% and 77.3% respectively. The area under the ROC curve consisting of the output value of artificial neura1 network was 0.837. Efficacy of the model was validated by means of blinded test. It is concluded that the ANN model is useful for clinical diagnosis of ITP on the basis of platelet protein fingerprint spectrum.
Adult ; Case-Control Studies ; Humans ; Neural Networks (Computer) ; Peptide Mapping ; Proteome ; analysis ; Proteomics ; Purpura, Thrombocytopenic, Idiopathic ; diagnosis ; genetics ; metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

Objective To establish a method for the simultaneous determination of ursolic acid(UA)and oleanolic acid(OA) in Ziziphora clinopodioides Lam.,and the quantitative determination the UA and OA contents in the different Z. clinopodioides plant samples collected with various parts of the plant at different times,from different regions of Xinjiang,China. Methods Dual wave?length scanning method was used for the quantification of UA and OA spots on a silica gel G plate in the TLC analysis. The samples loaded on the silica gel G plate were in situ treated with the 1%iodine solution in dichloromethane,and then the plate was developed using cyclohexane,cyclohexane-chloroform-ethyl acetate-formic acid(20:5:8:0.1)as the developing solvent. In the dual wavelength scanning,the measurement wavelength was 530 nm and the reference wavelength was 700 nm. Results The UA and OA spots in samples were well separated on the TLC plate and could be simultaneously quantified by the present method. The average contents of UA and OA in Z. clinopodioides plant samples from 18 different areas were(1.84 ± 0.41)and(2.82 ± 0.89)mg/g,respectively. The contents of UA and OA in the plant increased from late spring to early summer and then decreased thereafter. As to the different parts of the plant,the contents of UA and OA were highest in leaves and lowest in stems. Conclusion The method is simple,fast and accu?rate. The present results provided basic data for further evaluation of the quality of Z. clinopodioides resources.

OBJECTIVEThe distribution of upper airway obstruction sites can be identified quantitatively and dynamically with continuous airway pressure measurements in obstructive sleep apnea hypopnea syndrome ( OSAHS) patients. Its value as a clinical predictor for the outcome of revised uvulopalatopharyngoplasty was evaluated.

METHODSThe upper airway obstruction sites (transpalatal level, tongue base or hypolarynx level) were determined preoperatively with overnight upper airway pressure monitoring and concurrent polysomnography (PSG). Of one's total amount of obstructive events, the proportion of apnea/hypopnea events located at Transpalatal level was quantified as contribution of transpalatal obstruction. Of all OSAHS patients, 26 males and 1 female underwent revised uvulopalatopharyngoplasty (2 had same stage transpalatal advancement pharyngoplasty) and had follow-up PSG 6. 33 +/- 0.84 months after surgery. The relationship of transpalatal obstruction proportion, age, tonsil size and body mass index (BMI) and the reduction in apnoea hypopnea index (AHI) was analyzed.

RESULTSThe AHI (times/hr) of 27 subjects decreased from 63.9 +/- 20.7 to 28.4 +/- 25.4. The response rate was 51.9% (defined as AHI reduction over 50%). Correlation between the transpalatal obstruction proportion and the AHI reduction percentage was significant (r = 0.609), so was the degree of the tonsil size (r = 0.511). The proportion of tongue base level obstruction showed a negative correlation for the AHI reduction. Patients with oropharynx obstruction percentage > or = 70% had a success rate of 90% and all patients with oropharynx obstruction percentage <60% responded poorly to the operation. The regression model showed distribution of obstructive sites, along with tonsil size and other PSG parameters could predict 66.7% of the postoperative AHI of transpalatal level surgery (F = 6.701, P = 0.001) .

CONCLUSIONSContribution of transpalatal level obstruction has significant predictive value to the outcome of transpalatal level surgery in OSAHS patients.

Adult ; Airway Resistance ; Cleft Palate ; surgery ; Esophagus ; physiopathology ; Female ; Humans ; Male ; Middle Aged ; Monitoring, Physiologic ; Palate ; surgery ; Pharynx ; surgery ; Predictive Value of Tests ; Pressure ; Sleep Apnea, Obstructive ; physiopathology ; surgery ; Treatment Outcome ; Uvula ; surgery ; Young Adult

OBJECTIVETo prepare a cisplatin-impregnated coral-derived hydroxyapatite (CCHA) drug delivery system (DDS), and evaluate its inhibitory effect on human osteosarcoma cells U-2 OS, human breast cancer and prostatic carcinoma cells PC-3 in vitro.

METHODSThe coral-derived hydroxyapatite (CHA) was manufactured by hydrothermal exchange and impregnated with cisplatin by vacuum freeze-drying techniques. The leaching solutions of this DDS was collected at different intervals in a course of 8 weeks and their inhibitory effect on the cells was tested in vitro by MTT assay.

RESULTSElectron microscope showed that cisplatin was distributed homogeneously in the pores of CHA. The inhibition rates of the leaching solution on all the tumor cells exceeded 50% except for PC-3 cells, whose inhibition rate was 29.92% when treated with the solution collected at the eighth week.

CONCLUSIONCCHA allows sustained drug release and maintains excellent inhibitory effect on human bone tumor cells within 8 weeks in vitro.

Animals ; Anthozoa ; chemistry ; Antineoplastic Agents ; administration & dosage ; chemistry ; pharmacology ; Cell Line, Tumor ; Cell Survival ; drug effects ; Cisplatin ; administration & dosage ; chemistry ; pharmacology ; Drug Compounding ; methods ; Drug Delivery Systems ; Durapatite ; chemistry ; Humans ; Microscopy, Electron ; Time Factors

OBJECTIVETo understand how sleep stage and position influence the mechanisms for pharyngeal collapse in different levels of upper airway (UA), overnight state-related changes and postural variation in obstructive sites in obstructive sleep apnea hypopnea syndrome (OSAHS) patients were studied.

METHODSFifty four OSAHS patients underwent overnight upper airway pressure monitoring during polysomnography. The lower limits of the UA obstruction were determined and their relationship with sleep stage, position, age, body mass index and apnea hypopnea index (AHI) were investigated.

RESULTSAll 54 patients had oropharynx (14 837 of the 23 172 analyzed events) and tongue base obstruction (5605/23,172), 2532 events were located at the oropharynx with extension to tongue base. Twenty nine patients has hypopharynx obstruction (105/23 172). Of the total amount of apnea hypopnea, the portions of obstruction located at tongue base level increased (t = 8.790, P = 0.000) in rapid eye movement (REM) sleep while those located at oropharynx decreased (t = -6.846, P = 0.000). Indexes of the apnea hypopnea caused by tongue base obstruction raised (t = 6.189, P = 0.000). Although the overall AHI in supine position was higher than in lateral position (t = 4.000, P = 0.000), increases in indexes of both the apnea hypopnea caused by tongue base (supine, 17.1 +/- 13.8 vs. lateral, 13.9 +/- 14.6) and oropharynx obstruction (44.3 +/- 20.3 vs. 37.2 +/- 25.9) were without significance (P > 0.05). Distribution of obstructive site varied little with different position (P > 0.05).

CONCLUSIONSUpper airway obstruction involves more than one specific site of the upper airway and the oropharynx is the most common collapse site. Obstructive sites are likely to extend to lower levels during REM sleep. Sleep position has little effect on the distribution of obstructive site.

Adult ; Female ; Humans ; Male ; Middle Aged ; Pharynx ; pathology ; physiopathology ; Polysomnography ; Posture ; Sleep Apnea, Obstructive ; pathology ; physiopathology ; Sleep Stages ; Young Adult

BACKGROUNDSo far, in China, there has been no effective or easy procedure to define the control of asthma. This study assesses the validity of Asthma Control Test in Chinese patients.

METHODSThree questionnaires (Asthma Control Test, Asthma Control Questionnaire and the 30 second asthma test) were administered to 305 asthma patients from 10 teaching hospitals across China. Spirometry was also used. Asthma specialists rated the control of asthma according to patients' symptoms, medications and forced expiratory volume in first second. The patients were divided into noncontrolled group and controlled group according to the specialists' rating. Reliability, empirical validity and screening accuracy were conducted for Asthma Control Test scores. Screening accuracy was compared among 3 questionnaires. The patients' self rating and the specialists' rating were also compared.

RESULTSThe internal consistency reliability of the 5-item Asthma Control Test was 0.854. The correlation coefficient between Asthma Control Test and the specialists' rating was 0.729, which was higher than other instruments. Asthma Control Test scores discriminated between groups of patients differing in the percent predicted forced expiratory volume in first second (F = 26.06, P < 0.0001), the specialists' rating of asthma control (F = 88.24, P < 0.0001) and the Asthma Control Questionnaire scores (F = 250.57, P < 0.0001). Asthma Control Test showed no significant difference with Asthma Control Questionnaire in the percent correctly classified, while the percent correctly classified by Asthma Control Test was much higher than 30 second asthma test. The patients' self rating was the same as assessment of the specialists (t = 0.65, P = 0.516).

CONCLUSIONThe Asthma Control Test is an effective and practicable method for assessing asthma control in China.

Adult ; Aged ; Asthma ; diagnosis ; prevention & control ; therapy ; Forced Expiratory Volume ; Humans ; Middle Aged ; Spirometry ; Surveys and Questionnaires