BACKGROUND:In various clinical treatments of soft tissue defects, vacuum sealing drainage technology can effectively control wound infection, shorten the treatment time, and accelerate wound healing. OBJECTIVE:To further understand the features and characteristics of hydrated ethanol polyethylene foam vacuum sealing drainage treatment, and compare the treatment effects against soft tissue defects with the conventional dressing change treatment. METHODS:A total of 140 cases of large soft tissue defects were divided randomly into two groups. Observation group received hydrated ethanol polyethylene foam vacuum sealing drainage treatment, while control group was treated by conventional dressing change. The degree of pain relief during treatment, the family satisfaction, the survival rate of first skin-grafting, the time needed before skin-grafting, the hospitalization time, the number of operations, the readmission rates, the complication rate and other clinical effectiveness indicators were compared between the two groups. RESULTS AND CONCLUSION: In the observation group, visual analog scale scores decreased 5.76±0.68, in the control group visual analog scale score decreased 2.85±0.53, with significant differences between the two groups (P < 0.05), and family satisfaction degree of the observation group was higher (P < 0.05). The survival rate of first skin-grafting of the observation group was higher than the control group (P < 0.05), the time needed before skin-grafting, the hospitalization time, the number of operations, the readmission rates, and the complication rate were lower than the control group (P < 0.05). The results showed that, compared with conventional dressing change treatment, hydrated ethanol polyethylene foam vacuum sealing drainage has advantages of light pain, short treatment time, low rate of complications, and high efficacy.

Objective To find out the correlation between MMP-13 and clinicopathological parameters of breast cancer and identify clinical significance of MMP-13 overexpression on overall survival of breast cancer.Methods Immunohistochemistry was performed on paraffin-embedded tissue microarray containing 159 tissue dots from breast cancer patients.The intensity and the extent of IHC were scored by pathologists blind to clinicopathological parameters of the specimens.Different expression profiles of MMP-13 in breat cancer tissues and paraneoplastic tissues,and correlation between MMP-13 and breast cancer clinicopathological parameters were analyzed for statistical significance respectively.The impact of MMP-13 overexpression on overall survival of breast cancer.Results MMP13 expression were significantly higher in breast cancer tissues(54.4％) than in their corresponding paraneoplastic tissues(27.5％)(P =0.003).Expression of MMP-13 in breast cancer positively correlated with lymphma node metastasis(r =0.257,P =0.006),clinical TNM classification (r =0.310,P =0.001),HER2 expression (r =0.192,P =0.041).However,no significant correlation were oberserved between MMP-13 expression and tumor size,MMP-13 expression and tumor grade,MMP-13 expression and ER expression,MMP-13 expression and PR expression respectively.Conclusions Overexpress of MMP-13 is more common in breast cancer tissues than in their corresponding paraneoplastic tissues,and is an independent prognosis indicator of breast cancer.

Humans ; Neurofibromatosis 2

Ear, Inner ; surgery ; Humans ; Neuroma, Acoustic ; surgery ; Otologic Surgical Procedures ; methods

Humans ; Neuroma, Acoustic ; surgery

Inservice Training ; Otolaryngology ; education ; Physicians

BACKGROUND: Although cultured technique and purified method of Schwann cells (SCs) are changed successively,purity is still limited. Whether double negative selections can culture high-purified SCs still needs a further study.OBJECTIVE: To investigate the effects of double negative selections on growth and purity of SCs.DESIGN: Controlled animal study.SETTING: Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University,and the Beijing Institution of Otolaryngology.MATERIALS: The experiment was carried out in the Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University and the Beijing Institution of Otolaryngology between May 2006 and July 2006. Twenty new-born Wistar rats of 3 days (without gender limitation) were provided by the Experimental Animal Center of the Chinese Medical Academy.METHODS: The sciatic nerves of rats were digested by 0.2% collagenase. ① Grouping: All the rats were divided into 4 groups with 5 each. The experimental group was depurated by differential adhesion and Ara-c for 30 minutes. SCs in the control group 1 and 2 were managed by differential adhesion and Ara-c for 30 minutes, respectively. Cells in the control group 3 were not purified. ② Evaluation: The cell growth was observed under invert microscope; growth curves were measured with MTT method in the experimental group and control group 3 to observe cell proliferation. In addition, the SCs were identified by immunocytochemistry; the culture purity was calculated (ratio of S100 positive cells in each sight).MAIN OUTCOME MEASURES: ① Growth of SCs; ② immunocytochemistry stain of SCs; ③ growth curve of SCs; ④ purity of SCs.RESULTS: ① Growth of SCs: At 5 days after incubation, the SCs proliferated well. SCs were spindle, bipolar and sometimes tripolar, with obvious nuclei and a little cytoplasm. There was more fibroblast cell in the group 3 than that in experimental group. ② Growth curve: The SCs in experimental group entered into the logarithm multiplication period at the third day. ③ Immunocytochemistry: The positive-stained SCs were brown in its body and process, and arranged like swirl. The FB with spread and anomaly shape was slight blue. ④ The ratio of anti-S100 positive cells was higher in experimental group than that in group 3 (P ＜ 0.01).CONCLUSION: With the double negative selection, high-purified SCs are obtained effectively.

Objective To explore the efficacy of endoscopic electric cutters in the treatment of adenoidal hypertrophy .Methods 36 patients who underwent adenoidectomy treatment of adenoid hypertrophy were divided into the observation group and control group according to the random number table .The observation group was taken endo-scopic electric cutters treatment , the control group was taken traditional adenoid removal treatment .The therapeutic effect of the two groups was compared .Results In the observation group,the total time required for removal of the adenoids was (10.65 ±2.38)min,blood loss was (28.82 ±0.98)mL,compared with the control group,there were significant differences(t=25.93,31.79,χ2 =3.92,all P<0.05).In the observation group,there was 1 case had severe pain,but in the control group,there were 9 cases,the difference was significant (χ2 =8.86,P<0.05).In the observation group,the total effective rate was 94.45%,which was significantly higher than 55.55% in the control group(χ2 =7.26,P<0.05).Conclusion Compared to the traditional treatment of adenoid surgery ,electric cutters endoscopic treatment can significantly reduce adenoidal hypertrophy blood loss and the incidence rate of complication , improve patients'pain and clinical outcomes ,it is worth clinically further promotion .

Purpose To investigate the clinicopathological changes, immuniohistochemistry and molecular genetics phenotypic charac-teristics of the alveolar soft part sarcoma ( ASPS) . Methods 16 cases of ASPS were studied with clinicopathological, cytochemistry technique and immunohistochemical staining, two cases of ASPS were studied by FISH. Results There were 6 males and 10 females with the age 8~58 years (median age 31. 7 years). The tumors were located at limbs, shoulder and back, tongue, vocal cords, lung, cervix, and ureter. The clinical manifestations of the patients was a slowly growing mass. Histopathologically the tumor showed typical organ-like or acinar-like structure with sinus-like blood vessels and the fibrous septa formation. Sometime the clear or abundant eosino-philic granular cytoplasm of the tumors were obvious. The tumor cells had a crystalline substance formation by PAS staining. The tumor cells were positive for TFE3 and Cathepsin K by immunohistochemical staining. The ASPL-TFE3 gene fusion detection of tumor cells were present. Conclusion ASPS often located on the limbs of young patients. It may misdiagnosed as malignant epithelial tumors, primary or metastatic adenocarcinoma and paraganglioma when the tumor locate on a rare anatomical parts or an organs, such as tongue, vocal cords, cervix, ureter, etc. It is valueable that the typical alveolar-like structure of the tumor and the expression for TFE3 and Cathepsin K for the pathological diagnosis of ASPS. It is an important indicator that the ASPL-TFE3 gene fusion detection by FISH for the tumor.

The suppressive effect of anti-KDR antibody against VEGF on proliferation of hemangioma-derived vascular endothelial cells (HVECs) was investigated. HVECs from one case of hemangioma in proliferative phase were cultured. Both primary culture and sub-culture were conducted in M199 medium. The HVECs of passage 3 were divided into 4 groups based on the concentrations of anti-KDR antibody. Cell count was performed and inhibitory rate of HVECs was measured before and 9 days after interference. The results showed that the number of HVECs in the anti-KDR antibody-treated groups was significantly decreased and the inhibitory rate of HVECs by anti-KDR antibody (50, 10 and 2 microg/mL) was 84%, 63% and 39% respectively at 9th day after interference, with the difference being significant. In the control group, the number of HVECs was increased significantly. In was concluded that the anti-KDR antibody could suppress the activity of VEGF through blocking the KDR, indicating the potential clinical applications of anti-KDR antibody in the treatment of hemangioma.
Antibodies/*pharmacology ; Cell Proliferation/drug effects ; Endothelium, Vascular/*pathology ; Hemangioma/*pathology ; Tumor Cells, Cultured ; Vascular Endothelial Growth Factor Receptor-2/*immunology