3.Study on the regulation of HPAT axis molecular network in patients with aging-physiologic shen-deficiency syndrome.
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):841-843
Aging
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genetics
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physiology
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Diagnosis, Differential
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Humans
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Hypothalamo-Hypophyseal System
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physiology
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Kidney
;
physiology
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Medicine, Chinese Traditional
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Neuroimmunomodulation
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physiology
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Pituitary-Adrenal System
;
physiology
;
Thymus Gland
;
physiology
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Yang Deficiency
;
physiopathology
4.The application of optogenetics in the treatment of retinal degeneration disease
Yumeng SHEN ; Yiqiao XING ; Yin SHEN
Chinese Journal of Ocular Fundus Diseases 2016;32(3):338-342
Optogenetics is a novel technique which combines optics with genetics.Using genetic means,a selected opsin protein is ectopically expressed in target neurons,which are then stimulated by light to moderate the neuronal circuit,as a consequence to regulate the animal's behaviors.Retinal degeneration like retinitis pigmentosa and aged macular degeneration causes visual impairment and eventual blindness.Optogenetics techniques have opened the door to creating artificial photoreceptors in the remaining retinal circuits of retinal degeneration retinas via gene therapy.However,there are still limitations in optogenetics technique,for example,potential risk in virus infection,the choice of target cells and the low visual resolution of the experiment animal.It has been reported that vision was successfully restored to a certain extent in animal model using optogenetics technique.With higher photosensitivity of opsin protein,longer activation kinetics and higher transfection efficiency of virus vector,optogenetics techniques' application in ophthalmology will be improved.
6.Simultaneous determination of danshensu,protocatechuic aldehyde,caffeic acid,rosmarinci acid,salvianolic acid B,and salvianolic acid A in Xiangdan Injection by HPLC
Jicheng YUAN ; Yongqin YIN ; Zhibin SHEN
Chinese Traditional and Herbal Drugs 2009;0(S1):-
Objective To establish an HPLC method to determine simultaneously the content of six water-soluble chemical constituents (danshensu,protocatechuic aldehyde,caffeic acid,rosmarinci acid,salvianolic acid B,and salvianolic acid A) in Xiangdan Injection,and to compare their content discrepancy of Xiangdan Injection from 20 manufacturers.Methods The HPLC method was established.The column was Alltima C18 (250 mm?4.6 mm,5 ?m) at the gradient elution mode with the flow rate of 1.0 mL/min,column temperature was kept at 25 ℃ and the detector was set at 288 nm.Results Six components were separated clearly.The relationship between the concentration and the peak areas of the six compounds was all linear.The precision,stability,repetition,and average recovery were complied with the limit.Significant difference was found in contents of six chemical constituents in Xiangdan Injections from different manufacturers that the variation of salvianolic acid A content was the most distinct and danshensu was the most quietly.Conclusion The method has been successfully used to quanlify the six compounds in Xiangdan Injection,and then,can offer the reference for the quality control of Xiangdan Injections roundly.
7.Abnormal expression of costimulatory molecules CD_(28)and CD_(137)on PBMC from patients with chronic nephritis
Huaying SHEN ; Jixiang DONG ; Yin WANG
Chinese Journal of Practical Internal Medicine 2003;0(01):-
Objectives To investigate the expression features and roles of the costimulatory molecules and T lymphocyte subsets from patients with chronic nephritis.Methods The expression of the costimulatory molecules CD 28 and CD 137 on PBMC (peripheral blood mononuclear cells) and T lymphocyte subsets from 52 patients with chronic nephritis were studied by immunophenotyping and flow cytometry analysis.Results The T lymphocyte subsets from patients with chronic nephritis showed an obvious imbalance with reversing CD 4/CD 8 ratio,decreasing CD + 4T cells and increasing CD + 8T cells.The expression of the costimulatory molecule CD 28 on T cells was significantly lower compared with normal controls (P
8.Study of low dose and dynamic multi-slice CT about obstructive sleep apnea syndrome in sleeping
Jie SHEN ; Ji QI ; Jianzhong YIN
Chinese Journal of Radiology 2001;0(07):-
Objective To perform Low dose dynamic MSCT( multi-slice CT) in sleeping obstructive sleep apnea syndrome ( OSAS) patients correcting the imprecise measure values in waking state, and to exactly analyse the location and extension of the dynamic changes about the condition. Methods Sixteen OSAS patients were scanned both in waking and naturally sleeping period ( end phase of inspiration and expiration). Measured at the narrowest part of the retropalatal ( RP) and retroglossal ( RG) and 5 mm under the tip of epiglottis at the epiglottal ( EPG) at the end period of inspiration in sleeping, respectively, and compared the accurate pos(?)on of the narrowest or occlusive level in 3 phases. All patients were also scanned using cine mode at the narrowest level at the end period of inspiration in sleeping to show the pharyngeal cavity changes during sleep. Results The smallest XSA of RP region (Mw = 47. 50 mm2 ,Me =73. 00 mm2 , Mi =2. 00 mm2 ;Zwe =2. 897,Pwe =0. 003 ;Zwi =4. 192,Pwi
9.Clinicopathological analysis of malignant triton tumor of the posterior mediastinum
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(04):-
Objective To investigate the pathologic appearances,immunohistochemical features,and genetic changes of malignant triton tumor(MTT). Methods One case of MTT was studied pathologically and immunohistochemically,and the related literatures were reviewed. Results A huge mass,demonstrated in the thorax by X ray and CT scan was seen in the posterior mediastinum in the surgery.Histologically,the tumor was composed of spindle cells with significant atypia.Some of the tumor cells had dense eosinophilic cytoplasm.Immunohistochemical staining revealed positive for myoglobin,desmin and S-100 in most of the tumor cells.The pathological diagnosis was MTT of the posterior mediastinum. Conclusion Cases of MTT in the mediastinum are very rare,with less specific clinical and imaging manifestations.The diagnosis is mainly made on the basis of pathological examination and immunohistochemical staining.
10.Study on the characteristics of α-glucosidase inhibitor from Schizandra Chinensis
Linfeng XU ; Zhongming SHEN ; Jianwei YIN
Chinese Journal of Biochemical Pharmaceutics 2001;22(3):127-129
Purpose The aim is to study α-glucosidase inhibitor from Schisandra Chinensis(Turcz.)Baill.Methods The inhibitor was purified by soaking,ultrafiltration,ion-exchange chromatography and Lead Acetate deposition.Results A α-glucosidase inhibitor was obtained.Conclusion The inhibitor was a kind of non-competition inhibitor with large molecular weight of over 5×105D.