3.Study on the regulation of HPAT axis molecular network in patients with aging-physiologic shen-deficiency syndrome.
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(9):841-843
Aging
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genetics
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physiology
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Diagnosis, Differential
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Humans
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Hypothalamo-Hypophyseal System
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physiology
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Kidney
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physiology
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Medicine, Chinese Traditional
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Neuroimmunomodulation
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physiology
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Pituitary-Adrenal System
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physiology
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Thymus Gland
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physiology
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Yang Deficiency
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physiopathology
4.The application of optogenetics in the treatment of retinal degeneration disease
Yumeng SHEN ; Yiqiao XING ; Yin SHEN
Chinese Journal of Ocular Fundus Diseases 2016;32(3):338-342
Optogenetics is a novel technique which combines optics with genetics.Using genetic means,a selected opsin protein is ectopically expressed in target neurons,which are then stimulated by light to moderate the neuronal circuit,as a consequence to regulate the animal's behaviors.Retinal degeneration like retinitis pigmentosa and aged macular degeneration causes visual impairment and eventual blindness.Optogenetics techniques have opened the door to creating artificial photoreceptors in the remaining retinal circuits of retinal degeneration retinas via gene therapy.However,there are still limitations in optogenetics technique,for example,potential risk in virus infection,the choice of target cells and the low visual resolution of the experiment animal.It has been reported that vision was successfully restored to a certain extent in animal model using optogenetics technique.With higher photosensitivity of opsin protein,longer activation kinetics and higher transfection efficiency of virus vector,optogenetics techniques' application in ophthalmology will be improved.
6.Clinical effect of sufentanil and levobupivacaine on postoperative analgesia in pediatric caudal block anesthesia
International Journal of Pediatrics 2014;41(2):181-184
Objective To invetigate the effect and safety of sufentanil mixed levobupivacaine on postoperative analgesia in pediatric caudal block anesthesia.Method Sixty pediatric patients (2 ~ 6 years old) who were undergoing elective abdominal surgery,such as repair hernia of high ligation,were randomly divided into three groups with 20 cases each.after intravenous induction,0.25% levobupivacaine was injected in sacrum tube in group Ⅰ,0.5 μg/ml sufentanil mixed 0.25% levobupivacaine and 1.0 μg/ml sufentanil mixed 0.25% levobupivacaine were injected in sacrum tube in group Ⅱ and group Ⅲ respectively.The analgesia effect,the analgesia time,recover time and adverse reaction were observed and recorded 2,4,8,12,16,24 hours after the surgery.Results The analgesia effect in group Ⅱ、Ⅲ were significantly better than the group Ⅰ when 4、8、12 hours after the operation(P <0.05),and the analgesia effect in groupⅢ were significantly better than the group Ⅱ when 8 hours after the operation (P < 0.05).There were no significant differences in three groups when 2、16、24 hours after the operation(P >0.05),the analgesia time in group Ⅱ、Ⅲ were significantly longer than the group Ⅰ (P < 0.05),and the analgesia time in group Ⅲ were significantly longer than the group Ⅱ (P < 0.05).There were no differences in the recovery time of three groups (P > 0.05).There were no adverse reactions in three groups.Conclusions 0.5 μg/ml and 1.0 μg/ml sufentanil mixed 0.25% levobupivacaine may be used on postoperative analgesia in pediatric caudal block anesthesia safely and analgesia effect and time were more better and longer than 0.25% levobupivacaine singly.The analgesia effect in group with 1.0μg/ml sufentanil mixed 0.25% levobupivacaine was the best in three groups with the fewest side effects.
7.Study on the characteristics of α-glucosidase inhibitor from Schizandra Chinensis
Linfeng XU ; Zhongming SHEN ; Jianwei YIN
Chinese Journal of Biochemical Pharmaceutics 2001;22(3):127-129
Purpose The aim is to study α-glucosidase inhibitor from Schisandra Chinensis(Turcz.)Baill.Methods The inhibitor was purified by soaking,ultrafiltration,ion-exchange chromatography and Lead Acetate deposition.Results A α-glucosidase inhibitor was obtained.Conclusion The inhibitor was a kind of non-competition inhibitor with large molecular weight of over 5×105D.
8.Longevity and cancer.
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(4):302-303
Animals
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Apoptosis
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genetics
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Gene Expression Regulation
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Humans
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Longevity
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genetics
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Medicine, Chinese Traditional
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Neoplasms
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genetics
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Rats
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T-Lymphocytes
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cytology
9.Cytological diagnosis of auto immune thyroid disease in adolescent
Zhiqiang YIN ; Youqin SHEN ; Yaolin KUANG
Chinese Journal of General Surgery 2000;0(11):-
ObjectiveTo assess fine needle aspiration (FNAC) in the early diagnosis for auto immune thyroid disease (AITD) in adolescent.Methods Seventy-one patients, age ranging from 9 to 23 years with thyroid enlargement underwent FNAC. Their smears were classified into 4 types according to the degree of degeneration of adenoidal cell and lymphocyte invasion by HE combining with rapid staining.The results were compared with B ultrasound and serum thyroid auto antibody measurement.ResultsB ultrasound showed hyperthyroidism in 12 cases, inflammation in 53, nodule change in 5, normal in 5. Thyroid function examination demonstrated hyperthyroidism in 22 cases, hypothyroidism in 9 cases, and euthyroidism in 40 cases. Auto thyroid antibody (TGAb,TMAb) were all beyond normal range except in 1 case, and TPOAb elevated in 37 cases. FNAC identified Grave′s disease in 14, Hashimoto′s diseases typeⅠ( HT-Ⅰ) in 35 cases,HT(Ⅱ) in 13,HT(Ⅲ) in 3, and HT(Ⅳ) in 6 cases. ConclusionsFNAC contributes to early diagnosis of AITD in adolescent for its safety, simplicity, rapidity and accuracy.
10.Clinical study of the insulin resistance and pancreaticβ-cell function in patients with gestational diabetes
Yin YANG ; Jie SHEN ; Gugen XU
International Journal of Laboratory Medicine 2014;(21):2891-2892
Objective To investigate the insulin resistance and the changes of pancreaticβ-cell function in order to improve the clinical diagnosis and therapy .Methods 99 cases of pregnant women in the late ministers were randomly selected for the study and divided into three groups(normal glucose tolerance group ,impaired glucose tolerance group ,and gestational diabetes group) .The in-sulin resistance and isletβ-cell function of the three groups were evaluated by HOMA model and its related indicators .Results The blood pressure and blood lipid level was not significant different among the three groups(P>0 .05) .Pairwise comparisons of glu-cose infusion rate ,the first phase insulin secretion ,the second phase insulin secretion ,fasting glucose ,fasting insulin ,HOM A-IR , and HOMA-βwere made among the three groups ,and there were difference with statistical significance(P<0 .05) .Conclusion The situation of insulin resistance and the dysfunction of pancreaticβ-cell in trimester pregnant women would become bad associated with the abnormal glucose tolerance .