1.Situation analysis for drug clinical trial institutions.
Yin-Ying CHEN ; Ping WU ; Jie WANG
China Journal of Chinese Materia Medica 2014;39(16):3208-3210
Drug clinical trial is an important link in the chain of new drug research and development. The results of drug discovery and development directly depend on the extent of standardization of clinical trials. Therefore, improving the quality of drug clinical trials is of great importance, and drug clinical trial institutions play a crucial role in the quality management of drug clinical trials. After years of development, the overall level of drug clinical trials has advanced rapidly in China, and a large number of clinical trials of traditional Chinese medicine have also been carried out. However, there is still a big gap between our country and developed countries. Therefore, for the construction and management of Chinese drug clinical trial institutions, there is still a long way to go. This study aims to analyze the current development of drug clinical trial institutions in China and explore the existing problems from three aspects, including current situations of institutional organization and management, regional and professional distributions, and quality control. And some suggestions are put forward finally, including support of traditional Chinese medicine, introduction of drug-risk management system, and construction of information management.
China
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Clinical Trials as Topic
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standards
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Drug Evaluation
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Drug Therapy
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standards
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trends
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Drugs, Chinese Herbal
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standards
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therapeutic use
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Humans
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Quality Control
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Research
2.Clinical Characters Analysis of Patients with Drug-induced Liver Injury Combined with Hepatitis B
Ying ZHU ; Ping WANG ; Tao YIN
China Pharmacist 2014;(10):1702-1704
Objective:To analyze the clinical features of the patients with drug-induced liver injury ( DILI) combined with hepati-tis B to improve the awareness and attention to the disease by medical workers. Methods:Using retrospective analysis, the patients di-agnosed with DILI combined with hepatitis B from a tertiary-hospital were analyzed from January 2013 to January 2014. Results:Total-ly 30 cases of DILI combined with hepatitis B were collected, the number of male patients was higher than that of female patients, and most of them were elderly patients. The symptoms included weak, anorexia, yellow skin and sclera, tired of the oil, nausea, abdominal distension and so on. The patients with severe liver damage accounted for 80. 0%, 16. 7% was with moderate liver damage, and 3. 3%was with mild liver damage. Thirteen cases were mixed type, ten cases were hepatocellular type, and seven cases were cholestatic type. Conclusion:Middle-aged men may be prone to the disease, and the clinical manifestations are complex and diverse. Severe liver damage is majority, and often shows as mixed liver injury.
3.Establishment of a multidrug resistance cell line from human laryngeal cancer cells and its biologic features
Wanzhong YIN ; Ping WANG ; Wei ZHU
Chinese Journal of Immunology 2010;26(2):129-131,135
Objective:To establish a multidrug resistance cell line from human laryngeal cancer cells by VCR.Methods:Human laryngeal cancer cells(Hep-2) were exposed in stepwise escalating concentration of VCR until the resistant cell(Hep-2/v) line was developed. The IC50 and the resistance folds of multidrug resistance were detected with MTT assay. The differences of cell cycle distribution and Rhodamine accumulation between Hep-2 and Hep-2/v cells were studied through flow cytometry. The MDR1 gene were detected through real-time quantitative RT-PCR, and the corresponding protein was detected through western blot.Results:Hep-2/v cells was established, which had stable resistance to VCR and a resistance index of 45;Hep-2/v cells exhibited cross resistance to many other chemotherapeutic agents and its doubling time was prolonged;The number of cells in G_0/G_1 phase was increased while in S phase decreased(P<0.05);Rhodamine accumulation in Hep-2 cells was much more than Hep-2/v cells(P<0.01);The expression of MDR1 were increased than that of Hep-2 cells(P<0.01).Conclusion:Hep-2/v cell line shows the typical multidrug resistance phenotype.It can be served as a model for the study of MDR.
4.DELETION ANALYSIS OF DNA FRAGMENT RM07 FROM HALOBACTERIUM HALOBIUM
Yin WANG ; Yu-Ping HUANG ; Zhen-Hong DUAN ; Ping SHEN ;
Microbiology 1992;0(01):-
The DNA fragment RM07 was isolated from halophilic archaea Halobacterium halobium, which can function as promoter not only in halophilic archaea, but also in Escherichia coli as eubacterial promoter. Sequencing analysis indicated that it possessed the typical consensus sequences (-35 and -10) of bacterial gene promoter, which was confirmed by further deletion analysis: With its -35 sequence deleted and -10 sequence left,DNA fragment RM07a nearly cannot initiate transcription;With its both -35 and -10 sequences,RM07b DNA fragment could be active as promoter at a level even higher than RM07. Our research also showed that the promoter function of RM07 fragment in Escherichia coli was under the control of environmental factors,especially its positive correlation with the increasing concentration of sodium chloride. Therefore, RM07 DNA fragment may be potential1 novel promoter source for constructing double-function vectors. It also has special significance in elucidating the issues of the fusing characteristics of archaea and lateral gene transfer between archaea and bacteria.
5.Visual Completion Negativity to Human Face Perception
Xiang-li CHU ; Yin-hua WANG ; Yu-ping WANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(3):185-187
ObjectiveTo explore the neural basis of visual completion for the occluded faces.Methods2 types of visual stimuli were presented randomly: pictures of complete faces,pictures of incomplete faces(the occluded part was the left-top quadrant).Subjects were instructed to perform a gender discrimination task while event-related potentials(ERPs) were recorded.ResultsCompared with complete faces,incomplete faces enhanced the amplitude of N1 on the occipito-temporal scalp bilaterally,with the right hemisphere advantage.The peak latency of N2 elicited by incomplete faces was delayed compared with that of complete faces.ConclusionThe enhancement of N1 is related to visual completion processing that requires additional time.
6.Mismatch Positivity: Mismatch Component in Visual Modality
Xiang-li CHU ; Yin-hua WANG ; Yu-ping WANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(4):277-279
ObjectiveTo investigate if there were potential components similar to auditory mismatch negativity (MMN) in the visual modality.MethodsEvent-related potentials (ERP) of 15 healthy adults were tested. Subjects were demanded to complete shape-matching task and double-matching task under 4 kinds visual stimuli including no-difference (Nd), contour-difference (Cd), shape-difference (Sd) and double-difference (Dd).ResultsWithin P1-N1 latent period (100~140 ms), three kinds of deviant stimuli (Cd, Sd, Dd) elicited a positivity, mismatch positivity (MMP). MMP amplitude elicited by Dd was a sum elicited by Cd and Sd (single-difference).ConclusionIn the visual modality, MMP elicited by warp stimulus may be an element similar to auditory MMN and reflect auto-processing of brain to visual stimulus.
7.Is it inflammatory linear verrucous epidermal nevus or linear psoriasis?
Bin YIN ; Yu-ping RAN ; Peng WANG ; Jebina LAMA
Chinese Medical Journal 2013;126(9):1794-1795
8.Pleomorphic adenoma of nasal cavity in fancy.
Yin-ping WANG ; Jun-ge ZHANG ; Yan-xia YU
Chinese Journal of Pathology 2005;34(4):251-251
Actins
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metabolism
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Adenoma, Pleomorphic
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Glial Fibrillary Acidic Protein
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metabolism
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Humans
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Infant
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Keratins
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metabolism
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Male
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Nasal Cavity
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pathology
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Neoplasm Recurrence, Local
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Nose Neoplasms
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metabolism
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pathology
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surgery
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Reoperation
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Vimentin
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metabolism
9.Case of Sjogren syndrome.
Yin-Ping WANG ; Qing ling WEI ; Zong-Quan ZHANG
Chinese Acupuncture & Moxibustion 2013;33(10):918-918
10.Guidining Role of Reproductive Medical Ethics Committee in Artificial Insemination by Donor Semen and Case Analysis
Baoli YIN ; Yuhui ZHANG ; Ping ZHANG ; Xue WANG
Chinese Medical Ethics 2015;(4):605-607
Through the analysis of the selected case discussion, put forward the current often encountered in clinical practice for pure artificial insemination about some ethical problems in the implementation process of tech-nology, current situation and aims at the discussion the reproductive committee is carried out in the guidelines for the essence of artificial insemination technology functions:to provide correlation ethics consultation to demanders, 2 it is to assisted reproductive technology, and the correlation research to carry out the supervision and guidance.