Objective To find a method to inherit the experiences of famous senior TCM physicians,through verification and inheritance research of Professor Tian Cong-huo's clinical experience on degenerative gonarthrosis treatment.Methods By means of database of famous senior TCM physicians and various data explore methods,we performed multi-group controlled verification on Professor Tian Cong-huo's acupuncture prescription on degenerative gonarthrosis,in order to investigate the experience thoroughly.Results The three groups treated by Professor Tian Cong-huo's acupuncture prescription got similar therapeutic effects,and patients' symptoms have been relieved remarkably.Compared with the baseline,the scores of the knee joint ache in the group of Professor Tian,Successor,Senior and Control were decreased 1.20 (t=9.00,P＜0.05),1.13 (t=15.76,P＜0.01),1.1 (t=15.99,P＜0.05) and 1 (t=10.42,P＜0.05),respectively.Significant improvements of pain relieves had been achieved in all groups.No significant difference was observed among the previous three groups(F=2.64,P＞0.05),which had obvious improvement effect in comparing with the control group(t=3.81,P＜0.01).Conclusion demonstrated that the group of Professor Tian,the successor,and the senior each did better in knee pain-relief than the control group.Conclusion Confirmed by our research,this acupuncture prescription proves to be repeatable,safe,reliable and stable.Perfoming inheritance and verification research of famous and senior TCM physicians could help juniors learn and investigate their predecessors academic thoughts and connotation,proceed to the next step,inherit the precious experience through verification and combination of theory and practice.

[Objective]To evaluated the effect of reconstruction of circular ligament with fascia lata for the treatment of old capitulum of humerus dislocation.[Method] Twenty-four cases were included in this study with ranged age from 5 to 14 years old.Surgical operation time was 6 months to 5 years after injury.All cases received recontruction of circular ligament with fascia lata,without ulna recection.Internal fixation was used to fix the humeroradial joint.[Result]Eleven cases were followed up for and 5.9 years(ranged,5-7 years).Six cases showed rotation restriction,with average of 69?.Four cases showed flexion-extension restriction with an average of 21?.Sublocaiton was found in 1 case.Unlar nerve injury was found in 2 cases.Kirschner wire broken was found in 4 cases.Simotaneous radial nerve injury was found in 1 case.[Conclusion] It should be taken very carefully if patient's age is over 13 years old.Fascia lata is satisfactory replacement for circular ligament.

Adolescent ; Adult ; Aged ; Cerebellar Neoplasms ; genetics ; Child ; Child, Preschool ; Chromosomes, Human, Pair 16 ; Female ; Humans ; Loss of Heterozygosity ; Male ; Medulloblastoma ; genetics ; Middle Aged

Objective:To study the effects of IL 6、IL 1? and TNF? on the expression of c fos and c jun,which may provide the foundation for a further study in clinic.Methods:Used the a model of primary culture of human fetal cerebral neurons in serum free medium,by DNA RNA dot blot hybrdization.Results:The expression of c fos and c jun was increased between 15 min to 30 min after stimulation,reached a maximum at 1 h and declined over the subsequent 1 h.Conclusion:IL 6、IL 1? and TNF ? have induce the expression of c fos and c jun during development of human fetal cerebral neurons in vitro at the level of transcription.

ObjectiveTo explore working memory in male patients with alcohol dependence.Methods50 male patients with alcohol dependence(AD group) and 50 healthy controls(HC group) were assessed by digit span test,trail making test and verbal fluency test.The visual-object,visual-verbal,visual-space working memory tasks were used to measure the difference of working memory between the two groups.ResultsCompared with HC group,alcohol dependence group showed significant impairments in performance of error number of visual-object,visual-verbal,visual-space working memory tasks (AD group:(3.76±2.20),3(2,5),(3.74±2.40));(HC group:(2.94±1.60),2(1,3),2(1,4))(t=2.133,P=0.035;Z=-2.400,P=0.016;Z=-2.654,P=0.008).Moreover,scores of Michigan alcoholism screening test were positively correlated with the error number of verbal working memory task (r=0.371,P=0.008).ConclusionThe male patients with alcohol dependence exhibit impairments in all aspects of working memory.

Objective:To observe the effect of CD40 siRNA on the changes in kidney,urinary protein and complement C3 of MRL/Lpr mice and explore its therapy on lupus nephritis.Methods: 16 female MRL/Lpr mice were randomly divided into control group,empty vector group,CD40-siRNA1 group and CD40-siRNA2 group.The vectors expressing siRNA against CD40 were injected by tail veil into MRL/Lpr mice,while MRL/Lpr mice in control group and empty vector group were injected with the same dose of PBS and pGFP-V-RS vector respectively.There were six times injection and every one day.The 24 hours urine output was gathered 24 hours before mice were killed.Fourteen days following administration,these mice were killed,tissue sections of kidney were observed if the signal of siRNA were expressed in kidney.The expression levels of CD40 mRNA and protein in kidney tissue of MRL/Lpr mice were detected by RT-PCR and immunohistochemistry methods respectively.At the same time,the pathological changes of the kidney were observed by haematoxylin-eosin ( HE) staining method.The 24 h urinary protein content was detected using the method of coomassie brilliant blue and the expression levels of complement C3 in serum were detected by Immunoturbidimetric assays.Results:The vector of CD40-siRNA was expressed in kidney of MRL/Lpr.The expression levels of CD40 mRNA and protein in kidney were lower in CD40-siRNA1 group and CD40-siRNA2 group than control group and empty vector group on the 14th day after last injection ( P<0.05).The inflammatory cells infiltration of kidney and some glomerular volume were significantly reduced in CD40-siRNA1 group and CD40-siRNA2 group than control group and empty vector group.The renal tubular swelling was alleviated in CD40-siRNA1 group and CD40-siRNA2 group than control group and empty vector group.The levels of 24 hours urinary protein were lower and the levels of complement C3 were higher in CD40-siRNA1 group and CD40-siRNA2 group than control group and empty vector group ( P<0.05). Conclusion:CD-40 siRNA can suppress the expression levels of CD40 mRNA and protein and decrease inflammatory cells infiltration in kidney of MRL/Lpr.Meanwhile after suppressing expression of CD40 mRNA and protein, it can reduce the content of 24 hours urinary protein and elevate the level of complement C3 in serum,which CD-40 siRNA can delay progress of the disease and protect kidney,so that it has therapy effect on lupus nephritis.

As a heritable regulation , epigenetics can regulate gene expression by other ways without changing the DNA sequence ,and change cell or individual phenotypes .DNA methylation is an issue in the field of epigenetics research.Recently,many studies have been demonstrated that the methylation of repetitive DNA ,spe-cific gene and CpG island and loss of imprinting play an important role in tumor occurrence .As the development of technological approaches to DNA methylation ,we have a more comprehensive understanding on methylation pat-terns.As specific markers,abnormal methylation sites in the genome can be used in the diagnosis ,treatment and prognosis predictor of disease .For tumor development caused by DNA methylation ,the application of demethylat-ing drugs have achieved good effect in clinical treatment .

Objective To study mutagenic effects of spaceflight on physiological and biochemical parameters of Rhodobacter sphaeroides and select high-yield mutants in co-enzyme Q10(CoQ10) production for providing the experimental and theoretical basis for industrial production through mutagenic effects of spaceflight.Methods Variations in stress resistance and CoQ10 production of isolated strains were studied,the strain Rhodobacter sphaeroides was taken back by a recoverable satellite after 15 d flight in space.Results Compared to the control,the strain was characterized by highter NaCl tolerance and higher stress resistance,as well as with broader scope in growth temperature and pH value after spaceflight.The mutant colonies appeared white or pink which was different from their original red.The CoQ10 production of mutant 10 strain was increased by 73.13% much higher than that of control.Conclusion Spaceflight mutagenic effects on Rhodobacter sphaeroides shows to be multi-factor compared to the traditional single-factor mutagenesis methods.It can enhance stress resistance and increase CoQ10 production of isolated strains,and can be utilized in industrial microbial mutagenesis and breeding in the future.

Objective To investigate correlation between seizures and uric acid level in the hippocampus in a mouse model with acute limbic seizures by pharmacological and genetic method. Methods Normal male C57BL/6 and mutant mice were used in this study,including urate oxidase overexpression (UOx-OE) and urate oxidase knock-out (UOx-KO) ;These mice were divided into the following groups,including the control,KA, All ,OE ,KO group respectively;during the experiment ,behaviors ,latency and duration time were recorded;dialysates were collected by microdialysis technique and uric acid level was detected by FPLC;Uric acid in the hippocampus,seizures status,latency and duration were compared. Results Twenty-four mice in total were enrolled and only 1 death occurred until the end of the study. Seizures state appeared after KA treatment. Compared to the KA group,uric acid and generalized seizures declined by the treatment of KA and All (P< 0.05) . Compared to the KA group,uric acid,latency and generalized seizures elevated in the KO group (P < 0.05). Compared to the KA group,there was no difference of latency,duration,partial and generalized seizures occurring in the OE group (P> 0.05). Conclusions Uric acid level in the hippocampus of mice may have effects on seizures ,in which it suggests that uric acid and its relevant signal pathway could be a potential therapy target in seizures clinically.

Objective: To observe the impact of mind-regulating acupuncture plus donepezil on the cognitive ability, mean cerebral blood flow velocity, event-related potential P300, and activities of daily living (ADL) in the aged patients with Alzheimer disease (AD).Methods: Sixty senile AD patients were divided into a treatment group and a control group following the envelope method for random allocation, with 30 cases in each group. Based on the conventional treatment of the internal medicine, the control group received oral donepezil, and the treatment group received oral donepezil plus mind-regulating acupuncture. After 4-week treatment, the two groups were evaluated by the mini-mental state examination (MMSE), Alzheimer disease assessment scale-cognitive part (ADAS-Cog), and ADL; changes in P300 and the mean cerebral blood flow velocity were also observed.Results: Before treatment, there were no significant differences in the scores of MMSE, ADAS-Cog, or ADL between the two groups (P>0.05). The MMSE score increased after treatment in both groups and was notably higher in the treatment group than in the control group, showing intra-group and inter-group statistical significance (P<0.05). After treatment, the ADAS-Cog and ADL scores dropped in both groups and were markedly lower in the treatment group than in the control group, also showing intra-group and inter-group statistical significance (P<0.05). Compared with the same group before treatment, the latency of P300 was shortened and the amplitude was extended in both groups, all with statistical significance (P<0.05); the latency was shorter and the amplitude was larger in the treatment group than in the control group after treatment, presenting significant between-group differences (P<0.05). The mean blood flow velocity accelerated after the intervention in both groups, and the differences were statistically significant (P<0.05); the improvement in the treatment group was more notable than that in the control group (P<0.05).Conclusion: Mind-regulating acupuncture plus donepezil can regulate the latency and amplitude of P300, increase cerebral blood flow, and improve the learning and memory abilities of AD patients.