Objective To study the effect of gamma globulin on serum immune index in neonates with neonatal pneumonia. Methods One hundred neonates with neonatal pneumonia were selected, and they were divided into control group and observation group by random digits table method with 50 cases each. The control group was treated with conventional treatment, and the observation group was treated with conventional treatment combined with gamma globulin. The treatment efficacy, various signs disappearing times, length of stay in hospital and immune indexes were compared between 2 groups.Results The total effective rate in observation group was significantly higher than that in control group:96.00% (48/50) vs. 72.00% (36/50), and there was statistical difference (χ2 = 10.71, P<0.01). The disappearing times of cough, crackles, perioral cyanosis and fever and the length of stay in hospital in observation group were significantly shorter than those in control group:(4.39 ± 1.46) d vs. (6.32 ± 1.61) d, (4.21 ± 0.93) d vs. (5.36 ± 1.08) d, (0.83 ± 0.15) d vs. (1.16 ± 0.22) d, (2.53 ± 1.04) d vs. (4.47 ± 1.13) d and (7.68 ± 0.87) d vs. (10.03 ± 1.18) d, and there were statistical differences (P<0.01). The IgG, IgA, IgM, CD4+and CD4+/CD8+levels after treatment in observation group were significantly higher than those in control group: (10.32 ± 2.86) g/L vs. (6.88 ± 0.83) g/L, (1.14 ± 0.13) g/L vs. (0.91 ± 0.25) g/L, (0.94 ± 0.21) g/L vs. (0.85 ± 0.27) g/L, 0.516 ± 0.068 vs. 0.385 ± 0.063 and 1.89 ± 0.35 vs. 1.57 ± 0.33, but the CD8+was significantly lower than that in control group:0.303 ± 0.046 vs. 0.349 ± 0.048, and there were statistical differences (P<0.05). Conclusions The gamma globulin can not only significantly improve the curative effect in neonates with neonatal pneumonia and promote the recovery of the clinical symptoms, but also improve the efficiency of cell immunity and humoral immunity ability. It is a kind of effective treatment.

Thyroid cancer is the most common malignant endocrine tumor.The xenograft nude mice model of thyroid cancer have played an important role in studying thyroid cancer.Different mouse model has distinctive characters and limitations,so it is important to choose a suitable mice model due to the experiment purpose.In this paper,the authors will address the research progress of human thyroid cancer xenograft nude models.

Objective To study the imaging features of cervical lymphnode enlargement so as to improve the diagnosis of cervical lymph node disease.Methods CT and MRI appearances of 66 patients with pathologically-proved cervical lymph nodes disease were comparatively analyzed.Results There were significant difference in distribution of the multiple metastatic lymph node and lymph node tuberculosis(P=0.006).The ring-shaped enhancement were found in 20 cases with metastatic lymph nodes,19 of which were shown as thin-wall enhancement,its rate was 95%, and 5 ring-shaped enhancement of lymph node tuberculosis were shown as thick-wall enhancement.The former of which was significant difference than that of the latter(P=0.000).Of all central necrosis,it was significant difference between the metastatic lymph nodes(78%) and lymphoma(36%)(P=0.018).Conclusion According to the imaging features of cervical lymph nodes enlargement,their location and distribution,all of them may be diagnosed correctly.

Objective Observing the process of cell aggregation and differentiating into myocardium after the P19 cells treated with different methods and dimethyl sulfoxide(DMSO) inducer.Methods P19 cells were cultivated with DMSO in suspension for 7 days in Petri dishes or dishes containing a thin layer of soft agar to form cell aggregation.Then the aggregates were plated on gelatin-coated culture dishes,and cultured without DMSO to 19 days.The beating of cells was observed.?-sarcomeric actin and cardiac Troponin T(cTnT) immunocytochemical stains were used to identify cell differentiation.Results Cell aggregations were cohering-cultured with growth culture medium after DMSO induced for 7days,spontaneous and rhythmically beating cells were present within the aggregation outgrowths at day 15,which were ?-sarcomeric actin-positive and cTnT-positive.By day 19,positive rates were about 26% and 15% respectively.Conclusion By using DMSO exposure combination prior aggregation can induce P19 cells differentiate into cardiac myocytes which can spontaneously and rhythmically beat.

AIM: To explore mitochondrial mechanisms of apoptosis induced by artesunate in human leukemia HL60 cells. METHODS: Human leukemia HL60 cell lines were treated by artesunate. The inhibitory effect on cell proliferation was assessed by MTT assay. Electron microscopy and DNA gel electrophoresis was applied to demonstrate the presence of apoptosis. The level of MMP, cell cycle were determined by flow cytometry (FCM). The activity of caspase 3 was observed by colorimetry. RESULTS: Art showed a dose dependent and time dependent manner on the growth of HL60 cells (P

Adolescent ; Humans ; Male ; Tooth Ankylosis ; etiology ; surgery ; Tooth Extraction ; methods ; Tooth Root ; Wounds and Injuries ; complications

Acupuncture Therapy ; instrumentation ; Adult ; Female ; Humans ; Male ; Middle Aged ; Muscle Strength ; Piriformis Muscle Syndrome ; physiopathology ; therapy

An indexing system is constructed,based on the implementation of prevention and control technology for chronic non-communicable diseases in community health service institutions,using both literature review and Delphi method,and using the Analytic Hierarchy Process (AHP) to calculate the weight of the indexing system.The system comprises three level-1 indexes,8 level-2 indexes and 33 level-3 indexes.The research proposed a transition from emphasis of therapeutic means to prevention,and emphasis on prevention and control technologies to the high-risk population in the prevention and control technology of chronic non-communicable diseases,along with development of techniques and measures to encourage behavioral changes of the population with chronic diseases.

To study the effect of high altitude on the expression of transforming growth factor beta 1 (TGF ?1) during the healing process of radius fracture, a rat radius fracture model was established at a high altitude. The expression of TGF ?1 in callus was detected using of immunohistochemistry method. It was found that the expression of TGF ?1 in the rats of high altitude group were decreased significantly compared with the low altitude group, especially high altitude might affect the on day 3 and week 3 ( P

Objective: To analyze the clinical characteristics of pulmonary embolism(PE) after hepatectomy in patients with hepatic tumors, and to summarize our experience on the diagnosis, treatment and prevention of the condition. Methods: From Jan. 2005 to Dec. 2009, a total of 13,054 person-times with hepatic tumor underwent hepatectomy in our hospital, and 4 cases developed PE after hepatectomy. The clinical characteristics, pathogenic mechanism, diagnosis and treatment of the 4 patients were retrospectively analyzed. Results: The incidence of PE was 0.03% after hepatectomy in the present cohort. PE developed from day 2 to day 6 after hepatectomy; the symptoms included shortness of breath, cyanosis, chest distress, cardiopalmus, sudden death, and decrease of oxygen partial pressure(PaO2) and saturation of blood oxygen(SaO 2). One (25%) of the 4 patients died. Conclusion: PE is a serious complication after hepatectomy for hepatic tumor. Emergent chest X-ray and D-Dimer examination can make early and clear diagnosis; the condition should be managed once it is confirmed.