Objective To understand brucellosis infective status of Shapotou District, Zhongwei City of Ningxia. Methods The methods of stratified random sampling of epidemiological survey were used among high risk groups in 2008, who underwent erythromycin red plate cohesion test(RBDT) and test-tube agglutination test (SAT), in accordance with the diagnostic criteria for confirming cases of brucellosis(GB 15988-1995). Results Totally an occupational group of 2480 brucellosis in 10 townships were investigated, and 604 sera were tested. Tweenty eight cases were infected, the infective rate was 4.64%(28/604), 15 cases were diagnosed as brucellosis patients, prevalence rate was 2.48% (15/604); cattle attendants had the highest infection rate of 6.45% (26/403). Conclusions Brucellosis epidemic in Shapotou District, Zhongwei City of Ningxia, and it is place related to vocation.

Objective To investigate the insulin resistance and the changes of pancreaticβ-cell function in order to improve the clinical diagnosis and therapy .Methods 99 cases of pregnant women in the late ministers were randomly selected for the study and divided into three groups(normal glucose tolerance group ,impaired glucose tolerance group ,and gestational diabetes group) .The in-sulin resistance and isletβ-cell function of the three groups were evaluated by HOMA model and its related indicators .Results The blood pressure and blood lipid level was not significant different among the three groups(P>0 .05) .Pairwise comparisons of glu-cose infusion rate ,the first phase insulin secretion ,the second phase insulin secretion ,fasting glucose ,fasting insulin ,HOM A-IR , and HOMA-βwere made among the three groups ,and there were difference with statistical significance(P<0 .05) .Conclusion The situation of insulin resistance and the dysfunction of pancreaticβ-cell in trimester pregnant women would become bad associated with the abnormal glucose tolerance .

To review the widespread outcomes of the treatment and prevention of amblyopia,especially for the recent large randomized controlled study.By evaluating the factors affecting the results of the trial,we expect this paper may contribute to the treatment and basic research of amblyopia.

Objective To study the influence of early intensive blood pressure (BP) lowering treatment on peri-he?matomal secondary neuronal injury and prognosis after acute intracerebral hemorrhage. Methods A randomised con?trolled trial consisting of 36 consecutive patients within 6 h of intracerebral hemorrhage onset were randomly assigned to intensive BP lowering group and guideline BP lowering group. All patients underwent MR spectroscopy at 72 h and were followed up 90 days. N Acetyl Aspartate/Creatine (NAA/Cr) and lactic acid wave around peri-hematomal at 72 h, neuro?logic function at early stage, and death and/or disability at 90d were compared between these two group. Results Spec?trum lines were obtained from thirty-one patients:14 from intensive BP lowing group and 17 from guideline BP lowering group. There was no significant difference in the percentage declines of NAA/Cr at 72 h between the two groups (13.3%± 4.2% vs. 11.9%± 2.8%, P = 0.308). There was no statistical differences either in neurologic function at 7 d or in death and/or disability at 90 d (P>0.05). Conclusions Early intensive BP-lowering treatment had no role on peri-hematomal secondary neuronal injury and prognosis after acute intracerebral hemorrhage. Further high-quality and large-scale ran?domised controlled trial are necessary to verify this result.

Objective To determine the effect of intravenous administration of vascular endothelial growth factor (VEGF) on myocyte apoptosis and the expression of P 53 , Fas, Bax and Bcl 2 in an experimental model rat with acute myocardial infarction Methods Thirty three male Sprague Dawley rats were subjected to left coronary artery ligation The rats were randomized to receive VEGF 165 heparin(treated group) or heparin saline(control group), respectively, 24 hours after surgery VEGF 165 (2 ?g/1 ml)with heparin(50 U)or heparin saline(50 U/1 ml)was administered daily via tail vein for 7 days(treated group, n=8;control group, n =10) and 14 days(treated group, n=7;control group,n =8)separately Apoptotic index and the expression of the P 53 , Fas, Bax and Bcl 2 in myocardium were measured at 9 or 16 days after coronary artery ligation Results The number of apoptotic myocytes in VEGF treated group was less than that in control group\[(10?2)% vs (28?2)%, P

Objective To investigate the effect of mental processing speeds of judging traffic lights varying in location and color meaning. Methods 20 healthy adult male (age: 18 ～30 years) were enrolled in this study. By simulation of traffic lights as picture stimuli, all subjects performed a Go/NoGo task separately under normal condition, location-reversed condition, color meaning-reversed condition and both location and color meaning-reversed condition randomly. The accuracy rate and reaction time of the behavior task were quantified. Results The average accuracy rate of the four conditions was 99. 13% , no differences were found in different traffic light colors and different conditions. In the normal condition,the subjects' reaction time(ms) of traffic lights were as fallowings: red light 491.59 ±71.74; green light 487.55 ±58. 11, no difference was found between the two traffic light colors (P=0.522). In the location-reversed condition, subjects' average reaction time(ms) was respectively for red light 606.29 ± 107.59, and green light 593.13 ± 102.45. In the color meaning-reversed condition, it was respectively for red light 572.08 ±78.75, and green light 592.43 ±87.58. Compared with the normal condition, subjects' reaction time was prolonged in the location-reversed condition (F(1,19)=59.77, P<0.01), and also in the color meaning-reversed condition (F(1,19) =52.55, P<0.01). When in the both location and color meaning-reversed condition, the subjects' reaction time (ms) of traffic lights was respectively 502.92 ± 65.27 for red light, and 508.97 ±63.40 for green light,the result came close to statistical significance(P= 0.065)compared with the normal condition. Conclusion A likely "conceptual constancy" is demonstrated on the basis of prolonging the brains' information processing when location and color information changes.

OBJECTIVETo observe the immediate antihypertensive effect of guasha combined with bleeding therapy for mild (grade I) hypertension.

METHODSThirty patients with mild (grade I) hypertension and 30 cases with normal blood pressure were compared. Areas and acupoints in governor vessel, meridian of foot-taiyang, meridian of hand-yangming and meridian of foot-yangming were scraped for 3 times, which was followed by bleeding therapy. The blood pressures after each guasha and bleeding therapy were recorded as well as the skin temperature in Dazhui (GV 14) after each guasha. The treatment was given once a week and totally 4 treatments were given.

RESULTSThere were significant antihypertensive effects after the first guasha, the second guasha and the third guasha and bleeding therapy (all P<0.01), in which guasha combined with bleeding therapy had the most significant antihypertensive effect (P<0.01). The skin temperature in Dazhui (GV 14) was obviously increased after three times of guasha (all P<0.01).

CONCLUSIONGuasha or combined with bleeding therapy has better antihypertensive effect for mild hypertension, which is likely to be related with warming stimulation on meridians and acupoints.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Blood Pressure ; Case-Control Studies ; Combined Modality Therapy ; Female ; Hemorrhage ; Humans ; Hypertension ; physiopathology ; therapy ; Male ; Meridians ; Middle Aged

Real-time free breathing cardiac cine imaging is a reproducible method with shorter acquisition time and without breath-hold for cardiac magnetic resonance imaging. However, the detection of end-diastole and end-systole frames of real-time free breathing cardiac cine imaging for left ventricle function analysis is commonly completed by visual identification, which is time-consuming and laborious. In order to save processing time, we propose a method for semi-automatic identification of end-diastole and end-systole frames. The method fits respiratory motion signal and acquires the expiration phase, end-diastole and end-systole frames by cross correlation coefficient. The procedure successfully worked on ten healthy volunteers and validated by the analysis of left ventricle function compared to the standard breath-hold steady-state free precession cardiac cine imaging without any significant statistical differences. The results demonstrated that the present method could correctly detect end-diastole and end-systole frames. In the future, this technique may be used for rapid left ventricle function analysis in clinic.
Exhalation ; Heart Ventricles ; pathology ; Humans ; Magnetic Resonance Imaging ; Respiration ; Ventricular Function, Left

Connexin 43 ; genetics ; Connexins ; genetics ; Heart Defects, Congenital ; genetics ; Homeobox Protein Nkx-2.5 ; Homeodomain Proteins ; genetics ; Humans ; Infant ; Infant, Newborn ; Mutation ; genetics ; Myocardium ; metabolism ; Transcription Factors ; genetics

OBJECTIVE: To summarize clinical experience of application of argon knife under electronic bronchoscope in the management of pediatric glottic and subglottic diseases. METHOD: Reports in the literature were reviewed. Outcome and prognosis of 4 cases with pediatric glottic and subglottic diseases managed with argon beam coagulator technique in Shanghai children's Medical Center since 2011 were retrospectively analyzed. RESULT: Granulation tissue hyperplasia in 3 cases with subglottic lesions did not recur after surgery. Scarring or stenosis were not found in the airway. Intubation of the trachea and tube of tracheotomy was pulled out successfully in all cases, restoring normal breathing. No lesion recurrence was found in 1 case with squamous cell moderate atypical hyperplasia one month after the surgery. CONCLUSION Electronic bronchoscope has advantages of clear image, fine and soft lens body, optional bending, angle conversion, accurate positioning. And the argon knife with small head diameter can pass the pediatric supraglottic. It can be manipulated precisely with short operation time and no damage to surrounding normal tissues. Its worth in popularization and application of management for with glottic and subglottie disease in pediatric patients.
Adolescent ; Argon ; therapeutic use ; Bronchoscopes ; Child ; China ; Cicatrix ; Humans ; Hyperplasia ; Intubation, Intratracheal ; methods ; Laryngeal Diseases ; surgery ; Lasers, Gas ; therapeutic use ; Neoplasm Recurrence, Local ; Prognosis ; Retrospective Studies ; Tracheotomy ; instrumentation