Angiography technique is one of the most important technique in the diagnosis of vascular diseases,espcially in the diagnosis and treatment of intracranial aneurysms,coronary arterial and extremity arterial diseases.Preliminary application of angiography has been carried out on the diagnosis and pre-operative evaluation of some kinds of tumors such as musculoskeletal tumors.In this review,current clinical application and progress,advantages and disadvantages of digital subtraction angiography,computed tomography angiography and magnetic resonance angiography on the diagnosis of musculoskeletal soft tissue tumors are described and analysed.

Objective To evaluate arthroscopic reconstruction of a torn anterior cruciate ligament with either a central-third bone-patellar tendon-bone autograft or a two-strand semitendinosus-gracilis autograft and compare the results of clinical testing, satisfaction and functional status. Methods A retrospective study was done on 49 cases undergoing reconstruction with an ipsilateral central-third bone-patellar tendon-bone autograft and 54 treated with an ipsilateral two-strand semitendinosus-gracilis autograft. Patients were followed up for an average of 31.6 months (24-48 months). At the time of final follow-up, patients in each group were evaluated by Lysholm knee score, International Knee Documentation Committee (IKDC) rating scale and KT-1000 arthrometer for ACL ligament stability. Results No statistical difference was found between the groups in regard of the Lysholm knee score, overall IKDC score or KT-1000 arthrometrical measurements that stand for patient satisfaction, knee function and objective stability, respectively. The patellar tendon procedure group had a significantly higher knee pain rate than the hamstring group (37% versus 11%). Conclusions The bone-patellar tendon-bone autograft and the two-strand semitendinosus-gracilis autograft can attain comparable results for anterior cruciate ligament reconstruction in regard to patient satisfaction, knee stability and knee function in the medium-term follow-up. However, reconstruction of anatomic attachment sites, firm initial fixation and a structured postoperative rehabilitation program may have more influence on clinical outcomes and knee stability.

Objective:To investigate the relationship between meaning in life and procrastination of college students, and whether time value plays an mediating role between them.Methods:A total of 605 college students in Guizhou completed general procrastination scale, time value scale and life meaning scale.SPSS 22.0 software and PROCESS maro program were used for descriptive statistics, correlation analysis and mediating effect exploration.Results:(1) The procrastination score of college students was (34.40±7.74), the score of time value was (33.17±4.41), and the score of meaning in life was(46.03±8.81). (2) Procrastination was negatively correlated with meaning in life and time value ( r=-0.38, -0.16 respectively, P<0.01). While meaning in life was positively correlated with time value( r=0.31, P<0.01). (3) The mediating effect of time value perception between meaning seeking and procrastination was 22.6%.The direct effect of meaning in life on procrastination was 77.4%. Conclusion:College students with high meaning seeking is less procrastination, and time value mediates the effect between procrastination and meaning in life.Improving college students' meaning in life is conducive to indirectly reducing procrastination through time value.

ObjectiveTo explore pathogenesis of headache after subarachnoid hemorrhage (SAH) whether related with immune inflammatory reaction in subarachnoid and observe the effect of immunosuppressive action of dexamethasone on headache.Methods80 patients who was consciousness and complained headache after SAH were randomly divided into four groups, treated only with mannitol, mannitol plus cerebrospinal fluid (CSF) replacement, intrathecal and vein injection with dexamethasone. Effects of four groups were observed.ResultsEfficiencies of four groups were respectively the mannitol group 27.27%, the permutation group 66.67%, the intrathecal group 92.36% and the vein group 30.00%. There was a significantly difference between the intrathecal group and other three groups, and the time of headache remission for intrathecal group was also longer than that of other three groups (P<0.01).ConclusionThe wide immune inflammatory responses in subarachnoid induced by degenerative and hemic CSF is likely main cause of headache after SAH and intrathecal injection with dexamethasone has an obviously effect.

For engineering an efficient butanol-producing Escherichia coli strain, many efforts have been paid on the known genes or pathways based on current knowledge. However, many genes in the genome could also contribute to butanol production in an unexpected way. In this work, we used Tn5 transposon to construct a mutant library including 1 196 strains in a previously engineered butanol-producing E. coli strain. To screen the strains with improved titer of butanol production, we developed a high-throughput method for pyruvate detection based on dinitrophenylhydrazine reaction using 96-well microplate reader, because pyruvate is the precursor of butanol and its concentration is inversely correlated with butanol in the fermentation broth. Using this method, we successfully screened three mutants with increased butanol titer. The insertion sites of Tn5 transposon was in the ORFs of pykA, tdk, and cadC by inverse PCR and sequencing. These found genes would be efficient targets for further strain improvement. And the genome scanning strategy described here will be helpful for other microbial cell factory construction.
Butanols ; chemistry ; DNA Transposable Elements ; Escherichia coli ; metabolism ; Fermentation ; Gene Library ; Hydrazines ; Industrial Microbiology ; Mutagenesis ; Open Reading Frames ; Organisms, Genetically Modified ; Polymerase Chain Reaction ; Pyruvic Acid ; chemistry

[Objective]To improve the diagnosis and treatment of acute fatal pulmonary thromboembolism(PTE)in orthopedic perioperation.[Method]Clinical manifestations,radiological features,laboratory examination data,treatment and prognosis of 12 patients diagnosed as acute fatal PTE in orthopedic perioperation from February,2002 to July,2005 were retrospectively analyzed.[Result]Nine patients received emergent thrombolytic and anticoagulant therapy,7 were alleviated and 2 died;3 patients with sudden cardiac arrest died after failed cardiopulmonary resuscitation.[Conclusion]Acute fatal PTE is a severe disease of orthopaedics.The realization and alert of the existence of acute fatal PTE must be increased.Early diagnosis and efficient treatment is the key to preventing from death.

Objective:To comprehend morphology changes of temporomandibular joint in adults with mandible deviation,and the correlation in these changes.Methods:The mesofault radiographs of temporomandibular joint were taken in 21 adult patients with mandible deviation.The data described morphology of temporomandibular joint were analysed.Results:In adult patients with mandible deviation,the condyle process of opposite side was anterior and inferior when compared with deflected side.The height of condyle process,the upper height of condyle process,the gradient of prosobevel of condyle process and the gradient of back bevel of glenoid fossa were augmented when compared to deflected side.The gradient of prosobevel of condyle process showed positive correlation to the prosoblank of joint and the deep of glenoid fossa,and the height of condyle process showed positive correlation to the upper height of condyle process in both sides.The gradient of back bevel of condyle process showed positive correlation to the gradient of back bevel of glenoid fossa in deflected side.The gradient of back bevel of condyle process showed negative correlation to the supper blank of joint and the height of articular tubercle in opposite side.Conclusion:There are some differences in morphology of both temporomandibular joint in adults with mandible deviation,and there is some correlation between these changes.

Primary hepatic malignant fibrous histiocytoma(PHMFH)remains extremely rare with less than 60 cases reported in literature.From October 1999 to May 2008,5 patients with PHMFH had been admitted to Yantai Cancer Hospital,Yantai Yuhuangding Hospital and the First Affiliated Hospital of Wenzhou Medical College,and the results of spiral CT were analyzed.Six tumors were detected,and the maximum diameter of the tumors was more than 8.5 cm.CT plain scanning revealed that all tumors were hypodense,4 patients with tumor necrosis and cystic degeneration,and the tumor in 1 patient wag with homogen density.Enhanced CT scanning showed marked enhancement of the solid component of tumor in 4 patients,slight enhancement in 1 patient,and a"fast in and fast out"sign in 5 patients.Hepatic bile ducts and portal vein were not involved.One patient was with portal lymph node metastasis and 1 with ioferior vena cava involvement.The characteristics of spiral CT imaging of PHMFH include tumor necrosis,cystis and invasion,as well as"fast in and fast out"sign,which could help to diagnose,although the ultimate diagnosis depends on histopathological examination.

Objective To study material basis of Zhou's prescription, components of effective parts Ⅰ in Zhou's prescription was gualitative and quantitative analyzed. Methods The components of effective part Ⅰ was identified by test-tube reaction and qualitative TLC method, the content of saponins and flavones was quantitative analyzed. Results Total content of the saponins and flavone are 1.07% and 0.85% in Zhou's Prescription. The purity of saponins and flavones in effective parts Ⅰ are 37.4% and 29.7%. Conclusion The main components of effective part Ⅰ in Zhou's Prescription were flavones and saponins.

0 05). Conclusions Microendoscopy discectomy via laminoctomy has advantages of less trauma and quicker recovery.It is safe and effective in the treatment of lumbar disc heniation with limited spinal stenosis.