Objective To evaluate the changes and characteristics of respiratory function and pulmonary function in patients with asbestosis by multi-slice spiral CT imaging.Methods 61 asbestosis patents and 30 healthy subjects were enrolled and divided into normal re spiratory function group,mild injury group and moderate/severe injury group according to forced vital capacity and forced expiratory volume in one second.The lungs were scanned at the end of inspiratory and expiratory phase,and the lung volume,lung density and small airway index were measured.Results There were significant differences in lung volume (inspiratory and expiratory volume,volume difference,and volume ratio) among the normal group,the mild injury group and the moderate/severe injury group (all P＜0.05).There were statistically significant differences in the mean lung density (lung density at the end of inspiratory and expiratory phase,lung density difference,lung density ratio) among the three groups (all P＜0.05).There were significant differences in the small airway indexes (tracheal wall thickness ratio at the end of inspiratory and expiratory phase,bronchial wall area ratio) among the three groups (all P＜0.05).Conclusions CT lung function imaging techniques can be used to assess the degree of respiratory dysfunction in asbestosis patients.With the development of pulmonary respiratory dysfunction,the total lung volume decreased,the amount of lungs gradually increased,emphysema gradually increased,and the thickness of small airway wall and alveolar fibrous hypertrophy gradually increased.

Objective To explore the potential therapeutic targets of radiotherapy in colorectal cancer via bioinformatics analysis of gene expression microarray.Methods The gene expression dataset GSE15781 was downloaded from the GEO database.The differentially expressed genes in radiotherapy treated colorectal cancer samples compared with those without radiotherapy,as well as differentially expressed genes in colorectal cancer samples without radiotherapy compared with normal colorectal tissues without radiotherapy,were obtained by R programming.The DAVID database was used for the functional enrichment analysis of the differentially expressed genes.Besides,protein-protein interaction (PPI) network of the overlapped genes between the two groups of differentially expressed genes were constructed based on the HPRD database.Results A total of 702 differentially expressed genes were obtained for the radiotherapy treated colorectal cancer samples compared with the ones without radiotherapy.Besides,126 differentially expressed genes in colorectal cancer samples without radiotherapy compared with normal colorectal tissues without radiotherapy were identified.Biological processes that related to cell adhesion,cell proliferation,as well as inflammatory response were found to be enriched in those differentially expressed genes.Moreover,16 overlaps were found between the two groups of differentially expressed genes,and in the PPI network of the 16 overlapped genes,PTGS2 and SDCBP2,etc,were closely correlated with other genes,which might be the important therapeutic targets of radiotherapy.Conclusions Some potential therapeutic targets of radiotherapy in colorectal cancer can be obtained by analysis of gene expression profiles,which may be very important for the study and treatment.

What the medical moral qualities of medical students in adult’s higher education are has an important relation to that of the medical personnel,for most of them come from basic medical units.Whether to develop their good medical moral qualities or to improve their abilities of cognition,conviction and practice,the final purpose is to arouse their enthusiasm of participating in educational reform.In order to enhance their medical moral characters,the courses of medical ethics need to reform entirely both in content and form.

Cognition ; Language ; Medicine, Chinese Traditional ; Meridians ; Metaphor ; Translations

AlM: To comprehensively evaluate of visual quality after epipolis laser in situ keratomileusis ( EPl-LASlK) in mild to moderate myopia.METHODS: Sixty cases ( 120 eyes ) undergone EPl-LASlK with equivalent diopter ( SE ) were divided into two groups:mild myopia group (60 eyes) and moderate myopia group ( 60 eyes ) . Objective visual acuity, aberration, contrast sensitivity and glare sensitivity were measured before and 1wk; 1, 6mo after operation, and made comparative analysis. RESULTS: Postoperative uncorrected visual acuity ( UCVA ) of two groups were better than that of preoperative(P<0. 05). Postoperative 6mo, there were significant differences on total high RMS between two groups ( P < 0. 05 ). Before operation and 6mo after operation, higher - order aberrations had statistically significant difference between two groups (P<0. 05). After 1wk and 1mo, there were statistical significances at the five frequencies between two groups (P<0. 05). After 6mo, the mild myopia group returned to preoperative levels, however, moderate myopia group in the high frequency region ( 18. 0c/d ) had significant difference with before surgery (P<0. 05). 1wk after surgery, there were statistically significant differences on the 3. 0, 6. 0, 12.0, 18. 0c/d frequency in both groups (all P<0. 05), 1mo after surgery, there were significant difference at 12.0, 18.0c/d frequencies (all P<0.05), after 6mo, both groups returned to preoperative levels, showed no significant difference.CONCLUSlON:Visual quality is poorer in mild myopia patients than that in moderate myopia at early stage after EPl-LASlK. Better visual quality is observed at mid-late stage.

An indexing system is constructed,based on the implementation of prevention and control technology for chronic non-communicable diseases in community health service institutions,using both literature review and Delphi method,and using the Analytic Hierarchy Process (AHP) to calculate the weight of the indexing system.The system comprises three level-1 indexes,8 level-2 indexes and 33 level-3 indexes.The research proposed a transition from emphasis of therapeutic means to prevention,and emphasis on prevention and control technologies to the high-risk population in the prevention and control technology of chronic non-communicable diseases,along with development of techniques and measures to encourage behavioral changes of the population with chronic diseases.

Objective To analyze the changes of energy metabolism-related proteins(enzymes) and transformation of energy metabolism in transplanted heart.Methods The hearts transplanted from Lewis to Wister rats as allografts and from Lewis to Lewis rats as isografts were used to estab- lish Ono model.The changes in proteins were observed by using proteomics technique and compared between allografts and isografts in the left ventricular myocardial tissues 2 and 8 weeks after heart transplantation.Peptide mass fingerprint map was obtained by using matrix-assisted laser desorption/ ionization time of flight mass spectrometry(MALDI-TOF-MS)and the matched proteins achieved by using Matrix Science software.Results Eighty proteins(enzymes)of total 65 differential proteins that regulated energy metabolism were identified in allografted hearts,which correlated to the Krebs cycle and electron transport system involving glycometabolism,fatty acid and branched-chain amino acids oxidation.Conclusion The energy metabolism in allografts heart was transformed:mitochondria function was decreased in Krebs cycle but enhanced in electron transport system and outer mitoehon- drial membrane permeability to ATP,accelerated in glycolysis and fatty acid oxidation,however, branched-chain amino acids oxidation were suppressed.

Objective To investigate the effect of α-lipoic acid on cerebral injury induced by cardiopulmonary bypass (CPB) in dogs. Methods Twelve adult healthy mengrel dogs of both sexes weighing 13.5-17.5 kg were randomly divided into control group (group C) and α-lopoic acid group (group L) (n = 6each). In group L α-lipoic acid 50 mg/kg was injected iv immediately before CPB. The animals were anesthetized with intraperitoneal 2.5% pentobarbital 25 mg/kg, intubated and mechanically ventilated. PaCO2 was maintained at 35-40 mm Hg. Femoral artery and vein were cannulated and Swan-Ganz catheter was inserted into pulmonary artery for MAP, CVP and CO monitoring and blood sampling. Blood samples were obtained immediately before aortic cross-clamping (T0, baseline), at 30 and 60 min after aortic cross-clamping (T1,2) and 30 and 60 min after aortic unclamping (T3,4) for measurement of plasma concentrations of TNF-α, S100β protein and neuron-specific enolase (NSE). Results In group C CPB significantly increased plasma TNF-α, S100β protein and NSE concentrations as compared with the baseline values at T0. Pretreatment with α-lipoic acid significantly attenuated CPB-induced increase in plasma TNF-α, S100β protein and NSE concentrations in group L. Plasma S100β protein and NSE levels were positively correlated with plasma TNF-α level. Conclusion Pretreatment with α-lipoic acid is effective in attenuating CPB-induced inflammatory response and cerebral injury.

ObjectiveTo analyze the association between the outcome of exophthalmos and the curative effect of hyperthyroidism in Graves disease after 131I therapy.Methods Five hundred and thirty-eight cases of Graves disease accompanied with exophthalmos and hyperthyroidism who received 131I therapy from January 2001 to January 2010 were reviewed retrospectively,and the association between the outcome of exophthalmos and the curative effect of hyperthyroidism was analyzed.ResultsThe effective rate of hyperthyroidism after 131I therapy was 94.8％ (510/538).The effective rate of exophthalmos after 131I therapy was 73.2％(394/538).And the effective rate of hyperthyroidism with exophthalmos was 71.6％ (385/538).The incidence of improvement,inefficiency,exacerbation was 75.5％ (385/510),21.0％ (107/510),3.5％( 18/510) in hyperthyroidism effective patients ( 510 cases ) and 32.1％ ( 9/28 ),50.0％ ( 14/28 ),17.9％ ( 5/28 )in hyperthyroidism ineffective and recrudescence patients(28 cases),there was significant difference between the two patients (P ＜ 0.05 ).The incidences of improvement,inefficiency,exacerbation were 78.1％(281/360),18.9％(68/360),3.1％(11/360) in hyperthyroidism cured patients(360 cases),78.2％(36/46),17.4％ (8/46),4.3％ (2/46) in hypothyroidism patients(46 cases),there was no significant difference between the two (P ＞ 0.05 ).The incidence of improvement,inefficiency,exacerbation was 86.4％ ( 229/265 ),9.8％(26/265 ),3.8％( 10/265 ) in mild exophthalmos after 131I therapy(265 cases),59.9％(106/177),35.6％(63/177),4.5％(8/177) in moderate and severe inactive exophthalmos after 131I therapy( 177 cases),there were significant differences in the inciclence of improvement and inefficiency between the two patients (P＜0.05).For patients with moderate and severe active exophthalmos,glucocorticoids therapy could obtain satisfactory effect.Conclusion131I therapy is an effective method for Graves disease with exophthalmos,and there is a significant association between the outcome of exophthalmos and the curative effect of hyperthyroidism.

Objective To observe the effects of serum containing the medicine of Tujian Mixture on phospho-Akt and phospho-PTEN of glomerular mesangial cell (MC) cultured in high concentrations of glucose. Method The glomerular mesangial cells from SD rat were divided into six groups:the normal control group, the group of MC cultured in high concentrations of glucose, and four other groups cultured in high concentrations of glucose + different concentrations of rat’s serum containing Tujian Mixture. After 72 hours, the content level of phospho-Akt (Thr308) and phospho-PTEN (Ser380) in MC was detected by using sandwich ELISA. Results The level of phospho-Akt (Thr308) in the group of MC cultured in high concentrations of glucose was significant higher than that in the normal control group (P