The effect of solubilization of phospholipid component of Chinese medicines on other active components in decoction is reported in this paper. The experi ment result showed that the dissolution rate of ephedrine and chlorogenic acid increased obviously as the content of phospholipid component in decoction rose.

With the tracer colloidal lanthanum,light micro- scope and transmission electron microscope in animal experiments,we studied the retinal pigment epithe- lium(RPE)barrier of 48 moderately severe cryoge- nic lesions on the retina of 21 rabbit eyes at dif- ferent intervals after cryopexy.Our findings showed that:1.the repair of disrupted RPE barrier by cryopexy was poor or incomplete,the regenerated RPE cells in restored RPE barrier of some cryogenic lesions showed degeneration and decompensation dur- ing the recovery period,and the tracer in choroidal capillaries leaked into the degenerated RPE cells and the subretinal space;2.the biologic effect of cryo- pexy on the retina was closely related to the acting time and the size of cryoapplication probe;and 3. the damaged grade of subretinal space caused by moderately severe cryopexy was comparable to Lincoff and Kreissig's criteria,but it was not absolute.

The IR determination of phospholipid content in Semen Zizyphi Spinosae and Fructus Psoraleae, both of which are tonics,was reported for the first time.In this method,using PC as standard sample, chloroform as solvent and the absorption band of P-O-C bond as measurement wavelength,the ab- sorptive degree was linear for concentration in ranging from 0.6mg/ml to 4.0mg/ml.The standard deviation was 0.0682.CV was 1。69%。Compared with the result obtained by colorimetric assay, this method was simpler,its result was more stable.

Objective To explore the clinical efficacy of methylprednisolone injection and magnesium sulfate injection in the treatment of severe bronchial asthma and the impact on inflammatory cytokines.Methods 87 cases of severe bronchial asthma were selected for the study,and they were divided into two groups according to the order of admission,the control group (43 cases ) was given methylprednisolone treatment,while the observation group (44 cases)was given methylprednisolone injection and magnesium sulfate treatment.The clinical treatment and inflammatory cytokine levels before and after treatment were compared between the two groups.Results The effective rate of the observation group was 93.18%,which was higher than 74.42% of the control group,the difference was significant(χ2 =10.33,P <0.05 ).After treatment,pulmonary functions of the observation group [FVC (3.72 ± 0.42)L,FEV1 (2.72 ±0.32)L and PEF(3.48 ±0.49)L/s]were increased significantly than before treatment(t =7.87,7.81,8.41,all P <0.05),and had significant differences compared with the control group(t =6.93,7.44, 7.20,all P <0.05).After treatment,inflammatory cytokines indicators of the observation group[IL -6 (67.44 ± 0.43)μg/L,TNF -α(80.32 ±7.34)ng/L,hs -CRP(3.32 ±0.89)mg/L]were significantly increased than before treatment(t =6.85,7.41,7.33,all P <0.05),compared with the control group,the differences were significant(t =6.55,7.04,6.45,all P <0.05).Conclusion The methylprednisolone combination with magnesium sulfate in treat-ment of severe bronchial asthma had significant effect,and it can effectively improve the airway inflammation,promote lung function in patients'recovery,and it has great clinical value.

Objective To discuss application value of intestinal electromyography in patients with hepatic cirrhosis and to observe intestinal myoeletric activity.Methods There were 65 cases of hepatic cirrhosis in expermental group and 30 cases in control group. All the cases were recorded body surface intestinal electromyography using gastrointestinal myoelectric analyzer(WCDF-4B, Hefei , P.R.China). The parameters included average peak range-Ap, main frequency-Fp, average over zero frequency-Fz and special wave form were surveyed.Results Sharp higher Fz abnormal rate and emergence rate of special wave form in hepatic cirrhosis group were higher than in control group,P0 05.Conclusions In hepatic cirrhosis group,Fz was much higher than that in control group. And the intestinal myoelectric activity increased in the patients with hepatic cirrhosis,which represented by appearance of characteristic wave form that was frequent micromigration wave as well as load wave. Those changes accounted for heighten and irregularity of excitability intestinal myoelectric activity. Moreover, it will increase for the incidence of abnormality of intestinal myoelectric activity along with damage degree of hepatic function. Intestinal electromyography is a reliable method to judge intestinal myoelectric activity, the method has characteristics of harmless,free pain, convenient, safty and steady.

A method for the infrared spectrophotometric determination of total ph-ospholipids in seed of Juglans regia and acinus of Citrullus vulgaris was reported. The average recovery of total phospholipid in this test was 97.2%, the CV was 1.66%. The contents of total phospholipid in seed of Juglans regia and acinus of Citrullus vulgaris were 438.46-528.85mg% and 241.78 -247.47mg% respectively. The rapid quantitative analysis of the distribution of various phospholipid components in these two specimens was carried out by using thin layer chromatographic scanning and the corrective method of absorbance proportional coefficient. The results showed that the main phospholipid components in both specimens were PC and PE. The fatty acid composition of the total phospholipids of both specimens were analysed by GC-MS-DS (gas chromatography-mass spectrometry-data system).

To change the old stereotype of using literal and data information for storage and ex- change in medico—scientific records and provide a new record version rich in word and picture,a gener- al model of multi—media control system of medical records and an information storage pattern have been developed and the feasibility of development methods explored.

Objective To explore the effects of anti-tumor immunity induced by dendritic cells (DCs) vaccine pulsed with PEP3-KLH (keyhole limpet hemocyanin) on induction of specific immunity against prostate cancers.Methods DCs were propagated from bone marrow of C57BL/6 mice in vitro with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4).On day 5 of culture, DCs were harvested and incubated with PEP3-KLH or KLH.Then the DC vaccine was inoculated into C57BL/6 mice by subcutaneous injection for three times with an interval of two weeks.One week after the last vaccination, the levels of IL-2, IL-12, and interferon (IFN)-γwere measured with enzyme-linked immunosorbent assay (ELISA) kit.The aforementioned immunized mice with DC vaccines were challenged with transgenic adenocarcinoma of mouse prostate (TRAMP)-C2 tumor cells, the tumor growth curve was drawn, and survival rate was checked and compared.The cytotoxic T lymphocyte (CTL) activity induced by DCs was tested with lactate dehydrogenase (LDH) release method.The percentages of CD3 + , CD4+ ,or CD8 + T cells in tumor-infiltrating lymphocytes (TILs) were determined with flow cytometry.Results PEP3-KLH-DC group stimulated the body and induced higher levels of secreted IL-2, IL-12, and IFN-γ compared to DCs control and KLH-DC groups (P ＜ 0.01).The tumor of mice vaccinated with PEP3-KLHDC grew significantly slower than that injected with DCs or KLH-DC (P ＜0.01).Compared to the others, the survival rate in PEP3-KLH-DC group raised remarkably (P ＜ 0.01).PEP3-KLH-DC group induced more outstanding specific CTL activities of killing tumor cells than DCs control group and KLH-DC group (P ＜ 0.01), and the cytotoxicities of TILs in PEP3-KLH-DC group was significantly enhanced (P ＜0.01).The percentages of CD3 + , CD4 + , or CD8 + T cells in TILs (40.9％, 34.1％) in PEP3-KLH-DC group were significantly higher than those in DC-KLH (27.3％, 5.2％) or DCs (26.2％, 5.1％) group.Conclusions PEP3-KLH-DC vaccine can inhibit effectively tumor growth, enhance long-term survival in mice,intensify the local immunologic function of tumor, and elicit and promote profound specific anti-prostate cancer cellular immune responses.

This paper deals with the analysis of the phospholipids and fatty acids in Hippocomnpus kelloggi Jordan et Synder, H. Histrix Kaup, II. trimaculalus Leach, II. kuda Bleeder, II. japonicus Kaup, Solenognathus hardwickii(Gray), Syngnathoides biacufcatus(Bloch) and Tracliyrliamphus serralus (Temminck &. Schlegel).The total phospholipids were extracted with Folch solvent and determined by molybdate blue spec-trophotometric method. The result showed that the contents of total phospholipids in these spccimins were 2. 56-7. 82mg/g.The nine kinds of phospholipid standards of absorbance proportional coefficient were detected in this paper. The phospholipid components were separated and determined by using thin - layer chro-matographic method and Vaskovsky solvent as a specific spray reagent. The quantitative analysis of the distribution of various phospholipids components in these specimen ts were carried out, by using TLC scanning and the corrective method of absorbance proportional coefficient. The resut showed that the Hippocampus contains about five phospholipids: lysophosphatidylcholine, sphingornyelin, phophatidylcholine, phosphatidylinositol, and phosphatidylethanolamine. The phosphatidylserine , phosphatidylglycerol, diphosphatidylglycerol and phosphatidic acid were more in Syugnathus than in Hippocampus, But in all these specimens, the main phospholipid components were lysophos- phatidylcholine, sphingornyelin and phosphatidylcholine.Fourteen fatty acid compositions in these specimens were analysed by GC -MS -DS. The result showed that the main fatty acid were hexadecanoic acid, 9 - octadecenoic acid, 8,11- octadeca-dienoic acid and 1,7,10,13,16,19 - docosahexaenoic acid.

Purpose:To study the mutual relationships between apoptosis,cell proliferation correlative gene PCNA and tumor suppressor gene P27 Kip1 as well as to investigate if they have something to do with the occurrence,development,and prognosis in esophagus squamous cell carcinoma.Methods:47 cases of primary esophagus squamous cell carcinoma were put into investigative group whose tumor samples from operation resection were studied. 20 cases with normal esophagus cell mucous membrane tissue were put into the control group. Expression level of PCNA,P27 Kip1 was detected by immunohistochemistry method (S-ABC) in both groups. Apoptotic index was detected by TdT mediated deoxyuridylic acid(dUMP)nicking end labeling (TUNEL method) in both groups.Results:①In the group of 47 cases of esophagus squamous cell carcinoma,there were 17 cases of positive expression in P27 Kip1 ,and the positive rate was 36.17% , average proliferation index(PI) was 45.86?19.11%,average apoptotic index is 1.05%?0.59%,ratio of PI/AI is 57.37? 45.17 ,all of which were statistically significant for difference between normal tissue and carcinoma in esophagus squamous cell (P