BACKGROUND:Calcaneal fractures are often accompanied with a height reduction in the calcaneus. Compared to the intra-articular fracture with much specific operation indications, there is no standard to the reduction of calcaneal height that needs a surgery in extra-articular fractures. Therefore, it is necessary to find a more quantitative operation indication. OBJECTIVE:To explore the changes in calcaneus stress distribution along with the varying calcaneal height through finite element analysis. METHODS:A three-dimensional finite element model of the normal ankle was developed. Four finite element models with the calcaneus height decrease in 5, 10, 15 and 20 mm were developed based on the normal ankle model. And the VonMises distribution of the calcaneus, the maximal stress and its location were respectively analyzed. RESULTS AND CONCLUSION:In the normal ankle model, the stress concentration was found in the posterior inner part of the posterior articular surface, the anterior inner part of the posterior articular facet and the calcaneocuboid articular surface. And the maximal stress was 16.608 MPa, which appeared in the posterior inner part of the posterior articular surface. The stress concentration in the calcaneocuboid articular surface disappeared when the calcaneal height decreased, and the maximal stress of the model with the calcaneal height decrease of 5, 10, 15 and 20 mm was 18.325, 19.674, 22.491 and 25.694 MPa, respectively. The results suggest that the VonMises of calcaneus concentrates and increases obviously when the calcaneal height changes. It seems that the calcaneal height should be restored to the normal level. When the reduction of the fracture is difficult, the decrease of the calcaneal height should be limited within 10 mm.

The IR determination of phospholipid content in Semen Zizyphi Spinosae and Fructus Psoraleae, both of which are tonics,was reported for the first time.In this method,using PC as standard sample, chloroform as solvent and the absorption band of P-O-C bond as measurement wavelength,the ab- sorptive degree was linear for concentration in ranging from 0.6mg/ml to 4.0mg/ml.The standard deviation was 0.0682.CV was 1。69%。Compared with the result obtained by colorimetric assay, this method was simpler,its result was more stable.

HP450 has thecharacteristic of cytochrome P450 and sensitivity to antihistmine drugs.With distinct raiiable regularity in the diverse disease modes of the rat,and some relativity to the development of liver disease,the expression and transcription of the HP450s gene have all changed in the course of the liver cancer. HP450 is possibly regarded as a new index of diagnosing liver cancer and a new target site of preventing and treating liver cancer

In this paper 100 cases of renal space-occupying lesions which had been examined before operation by sonography and verified by surgery and pathology were analysed retrospectively. Results showed 99 cases of renal space-occupying lesions and 1 case of retroperitoneal tumor. There were 2 eas-es of false negative and 1 case of false positive for the localization by ultrasound.Nephromas were found to be 92.92%(92/99)totally.in which rnalignant and benign tumors were 86.96%(80/92)and 13.04%(12/92)respectively.5 cases of misdiagnosis and 2 cases of pitfall in qualitative analysis were iden-dried by pathology.The rate of accuracy was 92%(92/100).

A method for the infrared spectrophotometric determination of total ph-ospholipids in seed of Juglans regia and acinus of Citrullus vulgaris was reported. The average recovery of total phospholipid in this test was 97.2%, the CV was 1.66%. The contents of total phospholipid in seed of Juglans regia and acinus of Citrullus vulgaris were 438.46-528.85mg% and 241.78 -247.47mg% respectively. The rapid quantitative analysis of the distribution of various phospholipid components in these two specimens was carried out by using thin layer chromatographic scanning and the corrective method of absorbance proportional coefficient. The results showed that the main phospholipid components in both specimens were PC and PE. The fatty acid composition of the total phospholipids of both specimens were analysed by GC-MS-DS (gas chromatography-mass spectrometry-data system).

AIM: To investigate the antidysmsenorrhea pharmacologic effects of Compound Yimucao Paste (Herba Leonuri, Rhizoma chuanxiong, Radix Angelicae Sinensis, Flos Carthami) METHODS: The methods of microcirculation and isolated uterus smooth muscle of rat were used. RESULTS: Compound Yimucao Paste could improve the microcirculation disturbance on the aspects of the diameter, the velocity of mice auricle microarterioles and microveins, inhibit the isolated uterus smooth muscle contraction and mouse twister induced by oxytocin. CONCLUSION:Compound Yimucao Paste has an obvious action of blood quickening and stasis transforming and inhibit the isolate and uterus smooth muscle contraction induced by oxytocin, which are contributed to its antidysmenorrhea effect.

Objective:To study the soft tissue inflammation of percutaneous implants using bacteria investigation method, so as to provide basic data for further clinical study of percutaneous implants. Methods: Implants were surgically placed into tibias of the goats. The inflammatory secretion from the peri-implant soft tissue was taken to perform bacteria test on 4, 6 and 9 weeks post-operation. Meanwhile, the soft tissue inflammatory reaction was also observed weekly. Results: 5 of the 16 implants were infected. The soft tissue showed tumefaction and ulceration 2 months post-surgery. As to bacteria investigation, the number of G.Streptococci increased when the inflammation was getting more severe. Conclusion: The extent of the soft tissue inflammation of percutaneous implants probably has positive correlation with the infection of G.Streptococci.

Objective To observe the effect of aminoguanidine by intraperitoneal injection on apoptosis of chondrocyte with osteoarthritis.Methods Totally 32 rabbits were divided into 3 groups at random.Both the experiment and the model groups were fixed by plaster cast in the right hind straighten limb,while the control group received no treatment.The experiment groups were injected with aminoguanidine in abdominal cavity,100mg/(kg?d),and the control groups were injected the same voluminal normal saline in abdominal cavity.At the 4th week,we killed 4 rabbits from the normal group,and 6 from the control group and the experiment group,respectively.At the 8th week,we put the rest to death.We drew the materials from each sample for pathological observation on the whole and under the light microscope by HE and immunohistochemical staining,and counted apoptosis cells by TUNEL for statistical analysis.Results Compared with the control group,the experiment group was more similar to the normal group in general observation and optical microscope observation by HE staining.Both iNOS positive expression cell by immunohistochemical staining and apoptosis cell calculation by TUNEL of the experiment group were fewer than those of the control group(P

[Objective]To evaluate the cytocompatibility of chitosan-alginate scaffolds through in vitro cytoxicity experiment,cell-material co-culture experiment and in vivo implantation test.[Method]Chitosan-alginate scaffolds with longitudinal,paralleled multi-channels were produced via freeze-drying. Bone marrow derived stromal cells (BMSCs) were cultivated with the leach liquor. The cytotoxicity of scaffold was analyzed using MTT assay after 1,3 and 5 days of culture.Scanning electron microscopy was conducted after 3,5 and 7 days of BMSCs culture on the chitosan-alginate scaffold in vitro. Immunofluorescence detection was performed after implanting BMSCs and chitosan-alginate scaffolds in vitro in acute hemi-transaction spinal cord injury.[Result]The cytotoxicity of chitosan-alginate scaffold was in grade 0-1. Scanning electron microscopic observation indicated that the cells adhered to and grew on the surface of scaffold,arranging in a directional manner after 3 days of co-culture. NF200 and NSE fluorescence detection proved that a great many BMSCs were survival in the scaffold after 6 weeks,and that some transplanted cells differentiated into neuron-like cells.[Conclusion]Chitosan-alginate scaffold has a satisfactory cytocompatibility and may be an ideal tissue engineering scaffold material.

Purpose:To study the mutual relationships between apoptosis,cell proliferation correlative gene PCNA and tumor suppressor gene P27 Kip1 as well as to investigate if they have something to do with the occurrence,development,and prognosis in esophagus squamous cell carcinoma.Methods:47 cases of primary esophagus squamous cell carcinoma were put into investigative group whose tumor samples from operation resection were studied. 20 cases with normal esophagus cell mucous membrane tissue were put into the control group. Expression level of PCNA,P27 Kip1 was detected by immunohistochemistry method (S-ABC) in both groups. Apoptotic index was detected by TdT mediated deoxyuridylic acid(dUMP)nicking end labeling (TUNEL method) in both groups.Results:①In the group of 47 cases of esophagus squamous cell carcinoma,there were 17 cases of positive expression in P27 Kip1 ,and the positive rate was 36.17% , average proliferation index(PI) was 45.86?19.11%,average apoptotic index is 1.05%?0.59%,ratio of PI/AI is 57.37? 45.17 ,all of which were statistically significant for difference between normal tissue and carcinoma in esophagus squamous cell (P