Objective To observe the efficacy of combination of pranoprofen and artificial tears in treatment of dry eye syndrome after recovery from acute conjunctivitis. Methods This study involved 206 eyes of 157 consecutive patients who had recovered from acute conjunctivitis after routine treatment between July 2008 and September 2010. All cases were randomly divided into artificial tears group (79cases,104eyes) : artificial tears eye drops alone and combination group(78cases,102eyes) : pranoprofen combined with application of artificial tears eye drops. All patients'symptoms, signs such as tear film break 2 up time (BUT ) ,Schirmer I test (SIT) , corneal fluoresce staining (FL) score at 3,7,14days after treatment Were observed. Results All patients finished the trial at the end of the follow-up duration. There were significant differences in symptoms, BUT and corneal fluoresce staining between the two groups after treatment,combination group was superior to artificial tears group in BUT and FL. Conclusion Pranoprofen combined with artificial tears eye drops is superior to artificial tears eye drops alone

Objective? ?To?analyze?the?risk?factors?of?delirium?in?patients?in?cardiac?surgery?intensive?care?unit?(CSICU).? Methods? A?prospective?observational?study?was?performed.?Patients?admitted?to?CSICU?of?Fujian?Medical?University?Union?Hospital?from?March?to?August?in?2017?were?enrolled.?The?combination?of?the?Richmond?agitation?sedation?scale?(RASS)?and?the?ICU-confusion?assessment?method?(CAM-ICU)?were?used?to?evaluate?delirium.?The?patient?was?assessed?on?the?second?day?after?CSICU?admission,?twice?a?day,?the?evaluation?was?stopped,?and?the?follow-up??observation?was?terminated?after?the?patient?was?discharged?from?CSICU.?The?patients?were?divided?into?two?groups?according?to?whether?delirium?occurred?in?CSICU.?The?general?and?clinical?treatment?data?(including?condition,?operation,?anesthesia?and?CSICU?treatment)?of?the?two?groups?were?compared.?The?related?factors?of?delirium?were?identified?by?univariate?analysis?and?multifactor?Logistic?regression?analysis.? Results? A?total?of?318?cases?were?included?in?this?study.?Among?them,?93?cases?had?delirium?and?the?incidence?of?delirium?was?29.2%.?It?was?shown?by?univariate?analysis?that?age,?history?of?hypertension,?type?of?surgery,?surgical?procedure,?American?Society?of?Anesthesiologists?(ASA)?anesthesia?classification,?usage?of?propofol,?plasma?transfusion,?red?blood?cells,?platelet?transfusion,?blood?loss,?operative?time,?cardiopulmonary?bypass?(CPB)?time,?myocardial?block?time,?acute?physiology?and?chronic?health?evaluation?Ⅱ?(APACHEⅡ),?duration?of?mechanical?ventilation,?the?length?of?intensive?care?unit?(ICU)?stay,?postoperative?usage?of?diazepam,?midazolam,?fentanyl,?morphine,?chlorpromazine,?etc.?which?were?related?to?delirium,?and?occupation?? (on-the-job?or?self-employed),?medical?insurance?(city?or?provincial?medical?insurance),?education?(primary?to?junior?high?school,?high?school?or?above)?could?reduce?the?risk?of?delirium.?Colinearity?diagnosis?was?performed?on?variables?with?statistically?significant?differences,?and?variables?with?variance?expansion?factor?(VIF)?

Objective:To investigate the effect of salvianolic acid C (SalC) on fibroblast-like synoviocytes and through the role of nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway.Methods:Rheumatoid arthritis-fibroblast-like synoviocytes (RA-FLSs) were exposed to different concentrations of SalC (0.1 μmol/L, 1 μmol/L, 5 μmol/L, 10 μmol/L, 20 μmol/L) for 24-72 h and measured for viability, proliferation, migration and invasion by Cell counting kit 8 (CCK-8) assay, wound-healing and transwell assay. The levels of Tumor Necrosis Factor-α (TNF-α), Interleukin-1 beta (IL-1β) and IL-6 were measured by enzyme linked immunosorbent assay (ELISA). Western blot was used to detect the expression of matrix metallopeptidase (MMP)-9, MMP-13, apoptosis-related proteins and Nrf2 mediated gene. Then we used ML385 to inhibit Nrf2 signaling pathway. RA-FLSs were measured for migration and invasion, and the expression of proteins related to apoptosis, inflammation and Nrf2 pathway.Results:Compared with the control group, SalC inhibited the cell migration significantly (0.1 μmol/L, 0.75±0.05, t=7.65, P<0.001; 5 μmol/L, 0.50±0.05, t=14.25, P<0.001; 10 μmol/L, 0.26±0.05, t=20.67, P<0.001) and invasion (0.1 μmol/L, 0.75±0.11, t=4.93, P<0.001; 5 μmol/L, 0.49±0.06, t=10.32, P<0.001; 10 μmol/L, 0.26±0.07 , t=14.96, P<0.001) of RA-FLs, reduced the levels of MMP-9 (0.1 μmol/L, 0.72±0.10, t=5.60, P<0.001; 5 μmol/L, 0.48±0.08, t=11.03, P<0.001; 10 μmol/L, 0.27±0.06, t=15.94, P<0.001) and MMP-13 (0.1 μmol/L, 0.77±0.06, t=8.66, P<0.001; 5 μmol/L, 0.58±0.06, t=11.03, P<0.001; 10 μmol/L, 0.32±0.13, t=14.22, P<0.001), and promoted apoptosis. SalC reduced the level of pro-inflammatory cytokines significantly ( P<0.001) and activated the expression of Nrf2 signaling pathway proteins Nrf2, CAT, NQO1, SOD1 and GSS ( P<0.001). After ML385 was used to interfere Nrf2, the levels of SalC on Nrf2 pathway proteins, such as Nrf2 (0.68±0.06, t=5.08, P<0.001), CAT (1.44±0.12, t=4.77, P<0.001), NQO1 (0.65±0.12, t=5.04, P<0.001), SOD1 (1.43±0.10, t=6.36, P<0.001) and GSS (1.42±0.10, t=7.60, P<0.001), as well as the levels of TNF-α [(260±22) pg/ml, t=13.75, P<0.001], IL-1β [(701±30) pg/ml, t=12.98, P<0.001], IL-6 [(180±10) pg/ml, t=16.38, P<0.001) were significantly reduced. In addition, ML385 inhibited the inhibition of SalC on cell migration and invasion (0.70±0.09, t=11.24, P<0.001; 0.64±0.04, t=8.03, P<0.001) and induction of apoptosis (24.4±1.8, t=23.02, P<0.001). Conclusion:SalC may inhibit cell activity and inflammatory response, promote apoptosis via the upregulation of Nrf2 signaling pathway. SalC may have therapeutic potential in RA. However, further investigation are needed in animal models and human.

Objective:To propose a method of cervical cytology screening based on deep convolutional neural network and compare it with the diagnosis of cytologists.Method:The deep segmentation network was used to extract 618 333 regions of interest (ROI) from 5, 516 cytological pathological images. Combined with the experience of physicians, the deep classification network with the ability to analyze ROI was trained. The classification results were used to construct features, and the decision model was used to complete the classification of cytopathological images.Results:The sensitivity and specificity were 89.72%, 58.48%, 33.95% and 95.94% respectively. Among the smears derived from four different preparation methods, this algorithm had the best effect on natural fallout with a sensitivity of 91.10%, specificity of 69.32%, positive predictive rate of 41.41%, and negative predictive rate of 97.03%.Conclusion:Deep convolutional neural network image recognition technology can be applied to cervical cytology screening.

Objective To explore the predictive value of combined detection of immunoglobulin binding protein 1(IGBP 1)in serum and urine in lupus nephritis(LN).Methods A total of 60 LN patients were selected as LN group,and 60 SLE erythematosus(SLE)patients in the same period were selected as SLE group,the serum IGBP 1 and urinary IGBP 1 levels between the two groups were compared,and the value of serum IGBP 1,urinary IGBP 1 and their combination in predicting LN was analyzed.Results Serum IGBP 1 and urinary IGBP 1 in the LN group were significantly higher than those in the SLE group(P＜0.05).Binary Logistic regression analysis showed that serum IGBP 1 and urinary IGBP 1 were risk factors for LN(P＜0.05).Receiver Operating Characteristic(ROC)curve revealed that the area under the curve(AUC)for serum IGBP1,urinary IGBP1,and their combined detection for LN was 0.856,0.834,and 0.902,respectively.The Youden index was 0.533,0.533,and 0.666,respectively.The sensitivity and specificity of serum IGBP1 were 85.0％and 68.3％,re-spectively.The sensitivity and specificity of urinary IGBP1 were 53.3％and 100.0％,respectively.The sensitivity and specificity of the combined detection were 78.3％and 88.3％,respectively.Con-clusion Serum IGBP 1 and urinary IGBP 1 are highly expressed in LN patients,and their combina-tion has a high predictive value for LN.

Objective To explore the predictive value of combined detection of immunoglobulin binding protein 1(IGBP 1)in serum and urine in lupus nephritis(LN).Methods A total of 60 LN patients were selected as LN group,and 60 SLE erythematosus(SLE)patients in the same period were selected as SLE group,the serum IGBP 1 and urinary IGBP 1 levels between the two groups were compared,and the value of serum IGBP 1,urinary IGBP 1 and their combination in predicting LN was analyzed.Results Serum IGBP 1 and urinary IGBP 1 in the LN group were significantly higher than those in the SLE group(P＜0.05).Binary Logistic regression analysis showed that serum IGBP 1 and urinary IGBP 1 were risk factors for LN(P＜0.05).Receiver Operating Characteristic(ROC)curve revealed that the area under the curve(AUC)for serum IGBP1,urinary IGBP1,and their combined detection for LN was 0.856,0.834,and 0.902,respectively.The Youden index was 0.533,0.533,and 0.666,respectively.The sensitivity and specificity of serum IGBP1 were 85.0％and 68.3％,re-spectively.The sensitivity and specificity of urinary IGBP1 were 53.3％and 100.0％,respectively.The sensitivity and specificity of the combined detection were 78.3％and 88.3％,respectively.Con-clusion Serum IGBP 1 and urinary IGBP 1 are highly expressed in LN patients,and their combina-tion has a high predictive value for LN.

Objective To analyze the effect of microRNA-22-3p(miR-22-3p)of extracellular vesi-cles derived from bone marrow mesenchymal stem cells on premature ovarian failure.Methods Follicular fluids were provided by premature ovarian failure patients with in vitro fertilization or the second-gen-eration in vitro fertilization treatment and normal volunteers with the same treatment for infertility due to male factors;the exosomes derived from bone marrow mesenchymal stem cells were isolated by dif-ferential centrifugation;the morphology,particle size and marker proteins of isolated exosomes were analyzed by transmission electron microscopy,NanoSight LM10 analyzer and Western blotting.Granulosa cells were treated with cyclophosphamide(CTX),exosomes,miR-22-3 mimics and corresponding controls,and were divided into CTX group,control group,exosome incubation group,PBS treat-ment group,CTX+miR-22-3p group,CTX+miR-NC group,CTX+Exosomes group,and CTX+PBS group;gene expression was detected by Western blotting and quantitative real-time polymerase chain reaction(qRT-PCR).The 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)reagent and flow cytometry were used to detect cell viability and apoptosis.Results The extracted exosomes had typical goblet vesicles,the exosome marker proteins C cluster of differentia-tion 63(CD63)and C cluster of differentiation 9(CD9)were expressed in the extracted exosomes,and exosome particle size was 80 to 150 nm;miR-22-3p was significantly lowly expressed in patients with premature ovarian failure and ovarian granulosa cells induced by CTX(P＜0.05),but was significantly highly expressed in ovarian granulosa cells incubated with exosomes derived from bone marrow mesenchymal stem cells(P＜0.05);the activity of granulosa cells in the CTX group was significantly lower than that in the control group,but was significantly higher in the CTX+miR-22-3p group or CTX+Exosomes group than that in the control group(P＜0.05);the apoptosis rate of granulosa cells in the CTX group was significantly higher than that in the control group,but was sig-nificantly lower in the CTX+miR-22-3p or CTX+Exosomes group than that in the control group(P＜0.05).Conclusion The miR-22-3p of extracellular vesicles derived from bone marrow mes-enchymal stem cells can inhibit ovarian granulosa cell injury induced by CTX.

Objective To analyze the effect of microRNA-22-3p(miR-22-3p)of extracellular vesi-cles derived from bone marrow mesenchymal stem cells on premature ovarian failure.Methods Follicular fluids were provided by premature ovarian failure patients with in vitro fertilization or the second-gen-eration in vitro fertilization treatment and normal volunteers with the same treatment for infertility due to male factors;the exosomes derived from bone marrow mesenchymal stem cells were isolated by dif-ferential centrifugation;the morphology,particle size and marker proteins of isolated exosomes were analyzed by transmission electron microscopy,NanoSight LM10 analyzer and Western blotting.Granulosa cells were treated with cyclophosphamide(CTX),exosomes,miR-22-3 mimics and corresponding controls,and were divided into CTX group,control group,exosome incubation group,PBS treat-ment group,CTX+miR-22-3p group,CTX+miR-NC group,CTX+Exosomes group,and CTX+PBS group;gene expression was detected by Western blotting and quantitative real-time polymerase chain reaction(qRT-PCR).The 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)reagent and flow cytometry were used to detect cell viability and apoptosis.Results The extracted exosomes had typical goblet vesicles,the exosome marker proteins C cluster of differentia-tion 63(CD63)and C cluster of differentiation 9(CD9)were expressed in the extracted exosomes,and exosome particle size was 80 to 150 nm;miR-22-3p was significantly lowly expressed in patients with premature ovarian failure and ovarian granulosa cells induced by CTX(P＜0.05),but was significantly highly expressed in ovarian granulosa cells incubated with exosomes derived from bone marrow mesenchymal stem cells(P＜0.05);the activity of granulosa cells in the CTX group was significantly lower than that in the control group,but was significantly higher in the CTX+miR-22-3p group or CTX+Exosomes group than that in the control group(P＜0.05);the apoptosis rate of granulosa cells in the CTX group was significantly higher than that in the control group,but was sig-nificantly lower in the CTX+miR-22-3p or CTX+Exosomes group than that in the control group(P＜0.05).Conclusion The miR-22-3p of extracellular vesicles derived from bone marrow mes-enchymal stem cells can inhibit ovarian granulosa cell injury induced by CTX.