Aim To investigate the protective effect of flavonoids from Radix tetrastigmae (RTFs)on lipopo-lysaccharide (LPS)induced acute lung injury (ALI) of aged mice and the mechanism.Methods Aged C57BL/6J mice were bronchially instillated LPS to in-duce ALI.RTFs were orally administered to treat ALI. After 3 days,bronchoalveolar lavage fluid (BALF) was collected to enumerate leukocytes with Wright-Gi-emsa staining,and to detect inflammatory cytokines with ELISA.ELISA and Western blot methods were al-so used to detect the expression of MAPKs and NF-κB in lung tissues.The activity of NF-κB in nucleic pro-tein extract was detected with TransAMkit.Results
ALI models were successfully induced through LPS in-stillation.RTFs significantly reduced leukocyte,espe-cially neutrophil infiltration in BALF,inhibited IL-1 β, IL-6,IL-1 2p40,TNF-αand sTNF-R1 secretion,and improved pathohistological change of lung tissues.Be-sides,RTFs significantly attenuated the phosphoryla-tion of p38MAPK,NF-κB and the activity of NF-κB. Conclusion RTFs inhibits LPS-induced ALI through p38MAPK and NF-κB pathway and exhibits significant anti-inflammation effect on aged mice.

ObjectiveTo to evaluate the effects of nimodipine on cerebrovascular function in subarachnoid hemorrhage(SAH) using cerebrovascular hemodynamic indexes (CVAI),and to study the clinical value of CVAI in the dosing of nimodipine after SAH.Methods58 patients with SAH were given nimodipine 0.25 μg/kg·min by intravenous drip(i.v) after admitted to hospital. CVAI and NIHSS were examind before and 1 d, 7 d, 14 d after intravenous. ResultsNimodipine improved cerebrovascular function significantly, including increasing cerebral blood velocity and flow, decreasing cerebrovascular resistance(R) and critical pressure(CP). For those patients with NIHSS worse and R value increased, increased dosing could decrease cerebrovascular resistance(P<0.05) and improve neurological function significantly(P<0.05). ConclusionIndividualization is necessary in dosing of nimodipine treatment of SAH. R value is a important index in adjusting the dose of nimodipine.

ObjectiveTo investigate the relationship between complement-3(C3) and progression of early cerebral infarction.Methods168 consecutive patients with acute cerebral infarction within the first 24 hours from onset were recruited.Fasting venous blood sample was drawn on the morning of the second or the third day after admission.C3 and other clinical chemistry variables were measured.The relationship of C3 and progressing stroke(PS) was analyzed by Logistic regression.Results68 cases(40.48%) were considered as PS.Level of serum C3 in PS patients was 1.28±0.20 g/L higher than in those with non-PS(1.16±0.25 g/L,P=(0.000)).A higher C3 accompanied a higher tendency of prevalence of PS(P=0.001).Logistic regression analysis showed that C3 was significantly correlated with PS(OR for each 1.0 g/L=7.27;95% CI=1.52～36.39; P=0.013).ConclusionElevated serum C3 may play an important role in the prevalence of PS.

Objective:To compare the clinical efficacy of laparoscopic pelvic floor three-level internal repair and stapled transanal rectum resection (STARR) in the treatment of male patients with intrarectal prolapse. Mlethds A total of 101 male patients with rectal intrarectal prolapse from Feb 2013 to Oct 2017 were enrolled into this study. Fifty-two patient in group A received laparoscopic pelvie floor three-level internal repair, and 49 patients in group B received STARR. The Wexner incontinence scale (WIS), Wexner constipation scale (WCS) score, gastrointestinal quality of life index (GIQLI) and degree of internal rectal prolapse (DIRP) were systematically evaluated before surgery and 3 months, 1 year and 3 years after surgery. Results:There were no significant differences in age, BMI, number of bowel movements(BM), WIS, WCS, GIQLI and DIRP between the two groups before surgery(all P>0.05). The WIS, WCS, GIQLI and DIRP in 3 months, 1year and 3 years after surgery in both two groups were significantly better than those before surgery ( t=20.169, 25.229, 27.278, 23.818, 23.489, 21.152, -3.550, -23.042, -22.901, 82.852, 40.915, 30.010, 11.323, 13.237, 11.452, 19.473, 18.647, 17.108, -8.791, -5.254, -5.846, 37.439, 30.598, 22.852, all P<0.001). The GIQLI in Group A was significantly better than that of group B at 1 year and 3 years after surgery ( P<0.001) but close to that in Group B at 3 months after surgery ( t=1.428, P=0.156). The WIS, WCS and DIRP in group A were significantly better than those in group B at 3 months, 1 year and 3 years after surgery, with statistical significance ( t=-8.243, -15.688, -20.193, -4.268, -4.768, -4.851, 11.329, 13.543, -5.399, -4.745, -4.598, all P<0.001). There was no signifcant difference in grade Ⅰ-Ⅲ complications between the two groups (χ 2=0.046, P=1.00). Conclusion:Laparoscopic pelvic floor three-level internal repair is more effective than transanal STARR in the treatment of male internal rectal prolapse.

Objective:To propose a method of cervical cytology screening based on deep convolutional neural network and compare it with the diagnosis of cytologists.Method:The deep segmentation network was used to extract 618 333 regions of interest (ROI) from 5, 516 cytological pathological images. Combined with the experience of physicians, the deep classification network with the ability to analyze ROI was trained. The classification results were used to construct features, and the decision model was used to complete the classification of cytopathological images.Results:The sensitivity and specificity were 89.72%, 58.48%, 33.95% and 95.94% respectively. Among the smears derived from four different preparation methods, this algorithm had the best effect on natural fallout with a sensitivity of 91.10%, specificity of 69.32%, positive predictive rate of 41.41%, and negative predictive rate of 97.03%.Conclusion:Deep convolutional neural network image recognition technology can be applied to cervical cytology screening.